A grafting method for maesa japonica

Abstract

The application discloses a grafting method for south-chuan-microcos paniculata, belongs to the grafting technical field of south-chuan-microcos paniculata, and grafts the south-chuan-microcos paniculata through the steps of rootstock selection and treatment, scion selection and treatment, grafting, binding, post-grafting management and the like, and coats the prepared healing accelerator at the grafting joint, so that the components in the healing accelerator jointly act, the influence of various adverse factors on the grafting survival rate is reduced, the grafting survival rate of the south-chuan-microcos paniculata is improved, and the problem of low grafting survival rate of the south-chuan-microcos paniculata is solved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of grafting of Artocarpus nanchuanensis, and particularly relates to a grafting method of Artocarpus nanchuanensis. BACKGROUND

[0002] Artocarpus nanchuanensis S.S.Chang, S.H.Tan et Z.Y.Liu is a evergreen tree of Moraceae Artocarpus, is a species of Artocarpus plants naturally distributed in the northernmost end of China, is one of the rarest endemic plants in China, and is determined as a "critically endangered" tree species in the China Species Red List in 2004, is one level higher than the endangered species such as national first-class protected plant silver fir and metasequoia. The tree species is an evergreen tree, has a straight and large trunk, has fine and hard texture, has a beautiful tree shape, has deep green leaves, and has obvious greening effect. The leaves, bark and fruits can be used as medicine, have the effects of clearing heat and detoxifying. The bark can be used for papermaking and artificial cotton. The fruits are rich in fruit acid and vitamin, are delicious and sweet when eaten raw, have a bread taste when cooked, and are preferred by birds and animals. Since the species is extremely rare and endangered, has high medicinal, edible, ornamental and timber values, has potential economic and social values, such as garden avenue greening, cultivation of new fruit industry, development of medicinal value, and has broad development prospects, and has great protection, scientific research, popularization and development and utilization values.

[0003] The wild resources of Artocarpus nanchuanensis are rare and have narrow distribution. Since the natural regeneration and propagation ability is extremely weak, the population quantity can be expanded only through artificial measures. Artocarpus nanchuanensis is mostly propagated by sowing, is sowed with picking in autumn, and is sowed in spring and subjected to sand storage. The seedling is covered with soil of 1-2 cm in open field sowing, but the sowing propagation seed is prone to water loss, so that the germination rate is greatly reduced. Artocarpus nanchuanensis can also be propagated by cutting, but the survival rate is low. The grafting propagation can keep the genetic characteristics of the scion, so that the seedling can keep the excellent quality of the original fruit, and the time of early fruiting is shortened. However, the survival rate of the grafting propagation seedling of Artocarpus nanchuanensis in the current propagation technology is mostly only 10-15%, and the grafting survival rate is low. The factors affecting the grafting survival rate of Artocarpus nanchuanensis are various, such as the influence of endogenous substances, the transmission of nutrients, the invasion of pathogenic bacteria and the like. For example, the endogenous substances such as caffeic acid ethyl ester and coumaric acid in Artocarpus nanchuanensis are easily oxidized to generate harmful substances in the grafting interface during the healing process, are accumulated in the grafting interface, affect the differentiation of callus, and reduce the grafting survival rate. Therefore, it is necessary to find a grafting method of Artocarpus nanchuanensis, reduce the influence of the adverse factors, and solve the problem of low grafting survival rate of Artocarpus nanchuanensis. SUMMARY

[0004] In view of this, the purpose of the present application is to provide a grafting method of Artocarpus nanchuanensis, and solve the problem of low grafting survival rate of Artocarpus nanchuanensis.

[0005] The application solves the above technical problems through the following technical means:

[0006] A grafting method of Meliosma nanchuanensis, the grafting method is as follows:

[0007] (1) Selection and treatment of rootstock: select Meliosma nanchuanensis saplings growing healthily, free of diseases and pests, and fully lignified as rootstocks, and perform stump treatment on the rootstocks;

[0008] (2) Selection and treatment of scion: select fruit-bearing mother trees of more than 8 years old, collect fruit-bearing branches of the current year growing in the upper part of the crown, free of damage and diseases and pests, as scions, immediately package the scions in preservative film and put them into a preservative box after collection, then tear the preservative film, put the scions into river sand with the cut end downward, and store in the shade;

[0009] (3) Grafting: grafting is performed from the middle of January to the beginning of February, the grafting method is selected as cutting grafting, and the grafting agent is coated on the grafting joint after grafting;

[0010] (4) Binding: the grafting joint coated with the grafting agent is tightly bound with grafting film, and the grafting is completed, and a grafted seedling is obtained;

[0011] (5) Post-grafting management: cover the grafted seedling with film, remove the sprouts growing on the rootstock after the scion survives, and perform normal water and fertilizer management.

[0012] Further, the diameter of the rootstock in step (1) is 0.5-1.2 cm, and the seedling age is 1-2 years.

[0013] Further, the stump treatment in step (1) is performed on the rootstock within 2 cm from the ground.

[0014] Further, the thickness of the scion is 0.5-1.2 cm, and the length is 15-20 cm.

[0015] Further, the humidity of the river sand in step (2) is 60-65%.

[0016] Further, when the scion is put into the river sand, the top part needs to be exposed from the sand surface by 5-10 cm.

[0017] Further, in step (2), the sand storage time of the scion is ≤10 days.

[0018] Further, in step (5), the grafted seedling is covered with film and kept warm, when the air temperature is higher than 25℃, the covering film is removed, a sunshade net is covered, and the grafted seedling is watered to prevent high temperature from burning the grafted seedling.

[0019] Further, the grafting agent comprises the following raw materials by weight:

[0020] 20-40 parts of phenethylamine, 20-40 parts of magnesium hydroxide, 20-30 parts of copper oxychloride, 1-2 parts of diallyl sulfide, 20-30 parts of 2-aminoethanesulfonic acid, 80-100 parts of charcoal powder, 300-500 parts of guar gum, 1-2 parts of sodium tetraborate.

[0021] Further, the preparation method of the healing-promoting agent is as follows:

[0022] A: add water to phenethylamine to prepare a 20wt% phenethylamine solution;

[0023] B: add water to diallyl sulfide, mix well, and then perform water bath in hot water at 40-60 DEG C, after heating and stirring for 5-7 min, add 2-aminoethanesulfonic acid and mix well, then add magnesium hydroxide and stir well to obtain a mixed solution;

[0024] C: add guar gum and sodium tetraborate to water, mix well, then heat to 75-85 DEG C, constant temperature stirring for 3-5 min, cool to room temperature, then add charcoal powder, phenethylamine solution, mixed solution and copper oxychloride, place in a homogenizer, homogenize at a speed of 500-700 r / min for 3-5 min to obtain the healing-promoting agent.

[0025] The healing-promoting agent is coated on the grafting interface, the phenethylamine, magnesium hydroxide and caffeic acid ethyl ester and coumaric acid in the healing-promoting agent are combined into salt substances, the formation of harmful substances is inhibited, and the differentiation of callus is promoted, so that the survival rate of Nan Chuan Mappia fordii grafting is improved.

[0026] After the generation amount of harmful substances of the grafting interface is effectively controlled, the rootstock and the scion can be better combined, the grafting interface can be better healed, but the nutrient substances of the rootstock need to be transported to the scion. Therefore, the addition of copper oxychloride in the healing agent can improve the activity of the phenylalanine ammonia lyase in the Broussonetia papyrifera of Nanchuan, thereby promoting the synthesis of lignin and the formation of tubular molecules of the Broussonetia papyrifera of Nanchuan, so that the water and nutrient substances between the rootstock and the scion can be smoothly transported, thereby improving the survival rate of the grafting of the Broussonetia papyrifera of Nanchuan. In addition, the infection of pathogenic bacteria can cause the grafting interface to rot and reduce the survival rate, therefore, the addition of diallyl sulfide in the healing agent can kill the bacteria and prevent the bacteria from infecting the grafting interface to cause the grafting interface to rot and reduce the survival rate. The charcoal powder in the healing agent can prevent the accumulation of bleeding liquid of the grafting interface and accelerate the healing of the grafting interface. The components in the healing agent disclosed in the application synergistically act to reduce the influence of adverse factors on the survival rate of grafting, but the healing of the grafting interface needs a certain time, and the direct spraying of the healing agent on the interface is easy to flow away and reduce the efficiency, therefore, the addition of guar gum in the healing agent to prepare a gel can make the components in the healing agent well combined at the grafting interface, continuously act until the grafting interface is completely healed, effectively improve the survival rate of the grafting of the Broussonetia papyrifera of Nanchuan, and solve the problem of low survival rate of the grafting of the Broussonetia papyrifera of Nanchuan.

[0027] Advantages:

[0028] 1. The grafting method of the Broussonetia papyrifera of Nanchuan disclosed in the application coats the healing agent on the grafting interface, the components in the healing agent synergistically act to reduce the influence of adverse factors on the survival rate of grafting, and the survival rate of grafting of the Broussonetia papyrifera of Nanchuan is effectively improved, and the survival rate of grafting reaches more than 60%.

[0029] 2. The healing agent disclosed in the application is in a gel form, which can make the healing agent better adhere to the grafting interface, prolong the action time, better promote the healing of the grafting interface, and further improve the survival rate of grafting. BRIEF DESCRIPTION OF DRAWINGS

[0030] Figure 1 : It is a picture of the grafted seedling of the experimental group 1 of the application. DETAILED DESCRIPTION

[0031] The application will be described in detail below in combination with specific embodiments and drawings:

[0032] The application discloses a grafting method of Broussonetia papyrifera of Nanchuan, and a healing agent is prepared before grafting, which is as follows:

[0033] Example 1: Preparation of healing agent

[0034] Take 30g phenethylamine, 30g magnesium hydroxide, 25g copper oxychloride, 1.5g diallyl thiosulfoxylate, 25g 2-aminoethanesulfonic acid, 90g charcoal powder, 400g guar gum, 1.5g sodium tetraborate, wherein the particle size of the charcoal powder is 10μm.

[0035] Preparation method:

[0036] A. Add water to phenethylamine to prepare a 20wt% phenethylamine solution;

[0037] B. Add 75g water to diallyl thiosulfoxylate, mix, and then heat in hot water at 50°C, heat and stir for 6min, then add 2-aminoethanesulfonic acid and mix well, then add magnesium hydroxide and stir well to obtain a mixed solution;

[0038] C. Add guar gum and sodium tetraborate to 2000g water, mix well, then heat to 80°C, constant temperature stirring for 4min, cool to room temperature, then add charcoal powder, phenethylamine solution, mixed solution, and copper oxychloride, place in a homogenizer, homogenize at a speed of 600r / min for 4min to obtain the healing accelerator.

[0039] Example 2: Preparation of healing accelerator two

[0040] Take 20g phenethylamine, 20g magnesium hydroxide, 20g copper oxychloride, 1g diallyl thiosulfoxylate, 20g 2-aminoethanesulfonic acid, 80g charcoal powder, 300g guar gum, 1g sodium tetraborate, wherein the particle size of the charcoal powder is 10μm.

[0041] Preparation method:

[0042] A: Add water to phenethylamine to prepare a 20wt% phenethylamine solution;

[0043] B: Add 50g water to diallyl thiosulfoxylate, mix, and then heat in hot water at 40°C, heat and stir for 5min, then add 2-aminoethanesulfonic acid and mix well, then add magnesium hydroxide and stir well to obtain a mixed solution;

[0044] C: Add guar gum and sodium tetraborate to 1500g water, mix well, then heat to 75°C, constant temperature stirring for 3min, cool to room temperature, then add charcoal powder, phenethylamine solution, mixed solution, and copper oxychloride, place in a homogenizer, homogenize at a speed of 500r / min for 3min to obtain the healing accelerator.

[0045] Example 3: Preparation of healing accelerator three

[0046] Take 40g phenethylamine, 40g magnesium hydroxide, 30g copper oxychloride, 2g diallyl thiosulfoxylate, 30g 2-aminoethanesulfonic acid, 100g charcoal powder, 500g guar gum, 2g sodium tetraborate, wherein the particle size of the charcoal powder is 10μm.

[0047] Preparation method:

[0048] A: add water to phenethylamine to prepare a 20wt% phenethylamine solution;

[0049] B: add 100g water to diallyl thiosulfoxylate, mix well, and then perform water bath heating in hot water at 60°C. After heating and stirring for 7min, add 2-aminoethanesulfonic acid and mix well, and then add magnesium hydroxide and stir well to obtain a mixed solution;

[0050] C: add guar gum and sodium tetraborate to 2500g water and mix well, then heat to 85°C, and stir at constant temperature for 5min. After cooling to room temperature, add charcoal powder, phenethylamine solution, mixed solution, and copper oxychloride, and place in a homogenizer. Homogenize at a speed of 700r / min for 5min to obtain the healing accelerator.

[0051] Comparative Example 1: Preparation of healing accelerator

[0052] In comparison with Example 1, the only difference is that no phenethylamine solution is added during the preparation of the healing accelerator in Comparative Example 1.

[0053] Take 30g magnesium hydroxide, 25g copper oxychloride, 1.5g diallyl thiosulfoxylate, 25g 2-aminoethanesulfonic acid, 90g charcoal powder, 400g guar gum, and 1.5g sodium tetraborate, wherein the particle size of the charcoal powder is 10μm.

[0054] B. Add 75g water to diallyl thiosulfoxylate, mix, and then perform water bath heating in hot water at 50°C. After heating and stirring for 6min, add 2-aminoethanesulfonic acid and mix well, and then add magnesium hydroxide and stir well to obtain a mixed solution;

[0055] C. Add guar gum and sodium tetraborate to 2000g water and mix well, then heat to 80°C, and stir at constant temperature for 4min. After cooling to room temperature, add charcoal powder, mixed solution, and copper oxychloride, and place in a homogenizer. Homogenize at a speed of 600r / min for 4min to obtain the healing accelerator.

[0056] Comparative Example 2: Preparation of healing accelerator

[0057] In comparison with Example 1, the only difference is that no magnesium hydroxide is added during the preparation of the healing accelerator in Comparative Example 2.

[0058] Take 30g phenethylamine, 25g copper oxychloride, 1.5g diallyl thiosulfinic acid, 25g 2-aminoethanesulfonic acid, 90g charcoal powder, 400g guar gum, 1.5g sodium tetraborate, wherein the particle size of the charcoal powder is 10μm.

[0059] Preparation method:

[0060] A. Add water to phenethylamine to prepare a 20wt% phenethylamine solution;

[0061] B. Add 75g water to diallyl thiosulfinic acid, mix, then heat in a water bath at 50°C, heat and stir for 6min, then add 2-aminoethanesulfonic acid and mix well to obtain a mixed solution;

[0062] C. Add guar gum and sodium tetraborate to 2000g water, mix well, then heat to 80°C, constant temperature stirring for 4min, cool to room temperature, then add charcoal powder, phenethylamine solution, mixed solution, copper oxychloride, place in a homogenizer, homogenize at a speed of 600r / min for 4min to obtain the healing accelerator.

[0063] Comparative Example 3: Preparation of healing accelerator

[0064] In contrast to Example 1, the only difference is that no copper oxychloride is added when preparing the healing accelerator in Comparative Example 3.

[0065] Take 30g phenethylamine, 30g magnesium hydroxide, 1.5g diallyl thiosulfinic acid, 25g 2-aminoethanesulfonic acid, 90g charcoal powder, 400g guar gum, 1.5g sodium tetraborate, wherein the particle size of the charcoal powder is 10μm.

[0066] Preparation method:

[0067] A. Add water to phenethylamine to prepare a 20wt% phenethylamine solution;

[0068] B. Add 75g water to diallyl thiosulfinic acid, mix, then heat in a water bath at 50°C, heat and stir for 6min, then add 2-aminoethanesulfonic acid and mix well to obtain a mixed solution;

[0069] C. Add guar gum and sodium tetraborate to 2000g water, mix well, then heat to 80°C, constant temperature stirring for 4min, cool to room temperature, then add charcoal powder, phenethylamine solution, mixed solution, place in a homogenizer, homogenize at a speed of 600r / min for 4min to obtain the healing accelerator.

[0070] Comparative Example 4: Preparation of healing accelerator

[0071] In contrast to Example 1, the only difference is that no diallyl thiosulfonate is added during the preparation of the healing accelerator in Comparative Example 4.

[0072] 30 g of phenethylamine, 30 g of magnesium hydroxide, 25 g of copper oxychloride, 25 g of 2-aminoethanesulfonic acid, 90 g of charcoal powder, 400 g of guar gum, and 1.5 g of sodium tetraborate were weighed, wherein the particle size of the charcoal powder was 10 μm.

[0073] Preparation method:

[0074] A. Water was added to phenethylamine to prepare a 20 wt% phenethylamine solution;

[0075] B. 2-aminoethanesulfonic acid was added to 75 g of water and mixed uniformly, and then magnesium hydroxide was added and stirred uniformly to obtain a mixed solution;

[0076] C. Guar gum and sodium tetraborate were added to 2000 g of water and mixed uniformly, and then heated to 80°C, and stirred for 4 min at constant temperature, and then cooled to room temperature, and then charcoal powder, phenethylamine solution, mixed solution, and copper oxychloride were added, and placed in a homogenizer, and homogenized at a speed of 600 r / min for 4 min to obtain the healing accelerator.

[0077] Comparative Example 5: Preparation of healing accelerator

[0078] In contrast to Example 1, the only difference is that no 2-aminoethanesulfonic acid is added during the preparation of the healing accelerator in Comparative Example 5.

[0079] 30 g of phenethylamine, 30 g of magnesium hydroxide, 25 g of copper oxychloride, 1.5 g of diallyl thiosulfonate, 90 g of charcoal powder, 400 g of guar gum, and 1.5 g of sodium tetraborate were weighed, wherein the particle size of the charcoal powder was 10 μm.

[0080] Preparation method:

[0081] A. Water was added to phenethylamine to prepare a 20 wt% phenethylamine solution;

[0082] B. 75 g of water was added to diallyl thiosulfonate, and then heated in a water bath at 50°C, and then heated and stirred for 6 min, and then magnesium hydroxide was added and stirred uniformly to obtain a mixed solution;

[0083] C. Guar gum and sodium tetraborate were added to 2000 g of water and mixed uniformly, and then heated to 80°C, and stirred for 4 min at constant temperature, and then cooled to room temperature, and then charcoal powder, phenethylamine solution, mixed solution, and copper oxychloride were added, and placed in a homogenizer, and homogenized at a speed of 600 r / min for 4 min to obtain the healing accelerator.

[0084] Comparative Example 6: Preparation of healing accelerator

[0085] In contrast to Example 1, the only difference is that no charcoal powder is added in the preparation of the healing agent in Comparative Example 6.

[0086] 30 g of phenethylamine, 30 g of magnesium hydroxide, 25 g of copper oxychloride, 1.5 g of diallyl thiosulfinate, 25 g of 2-aminoethanesulfonic acid, 400 g of guar gum, and 1.5 g of sodium tetraborate were weighed out, wherein the particle size of the charcoal powder was 10 μm.

[0087] Preparation method:

[0088] A. Water was added to phenethylamine to prepare a 20 wt% phenethylamine solution;

[0089] B. 75 g of water was added to diallyl thiosulfinate, which was mixed and heated in a water bath at 50°C. After 6 min of heating and stirring, 2-aminoethanesulfonic acid was added and mixed evenly, and then magnesium hydroxide was added and stirred evenly to obtain a mixed solution;

[0090] C. Guar gum and sodium tetraborate were added to 2000 g of water and mixed evenly, and then heated to 80°C. After constant temperature stirring for 4 min, the solution was cooled to room temperature, and then the phenethylamine solution, the mixed solution, and copper oxychloride were added. The mixture was placed in a homogenizer and homogenized at a speed of 600 r / min for 4 min to obtain the healing agent.

[0091] Comparative Example 7: Preparation of healing agent

[0092] In contrast to Example 1, the only difference is that no guar gum and sodium tetraborate are added in the preparation of the healing agent in Comparative Example 7.

[0093] 30 g of phenethylamine, 30 g of magnesium hydroxide, 25 g of copper oxychloride, 1.5 g of diallyl thiosulfinate, 25 g of 2-aminoethanesulfonic acid, 90 g of charcoal powder, 400 g of guar gum, and 1.5 g of sodium tetraborate were weighed out, wherein the particle size of the charcoal powder was 10 μm.

[0094] Preparation method:

[0095] A. Water was added to phenethylamine to prepare a 20 wt% phenethylamine solution;

[0096] B. 75 g of water was added to diallyl thiosulfinate, which was mixed and heated in a water bath at 50°C. After 6 min of heating and stirring, 2-aminoethanesulfonic acid was added and mixed evenly, and then magnesium hydroxide was added and stirred evenly to obtain a mixed solution;

[0097] C. The charcoal powder, the phenethylamine solution, the mixed solution, and copper oxychloride were mixed and placed in a homogenizer, and homogenized at a speed of 600 r / min for 4 min to obtain the healing agent.

[0098] Example 4: Grafting method of Alstonia roxburghiana

[0099] (1) Stock selection and treatment: select the healthy, disease-free and fully lignified 2-year-old Nanchuan Paulownia seedlings with a diameter of about 1 cm as the stock, and cut the stock at about 1.5 cm from the ground;

[0100] (2) Selection and treatment of scion: select fruit-bearing mother trees of more than 8 years old, collect the current year's fruit-bearing branches growing in the upper part of the crown without damage and disease and insect pests as scions, the thickness of the scion is about 1 cm, and the length is about 17 cm. The scion is immediately packaged with preservative film after collection and transported to the grafting point in a preservative box, then the preservative film is torn open and the scion is placed in river sand with a water content of 60% with the cut end down, the top is exposed 7 cm above the sand surface, and it is stored in the shade;

[0101] (3) Grafting: grafting is carried out in early February using the conventional cutting method, and the grafting interface is coated with the healing accelerator prepared in Example 1, so that the healing accelerator uniformly and completely covers the grafting interface, and the coating thickness of the healing accelerator is about 0.5 cm;

[0102] (4) Binding: the grafting interface coated with the healing accelerator is tightly bound with the conventional grafting film after grafting to complete the grafting, and the grafted seedlings are obtained;

[0103] (5) Post-grafting management: after grafting, the grafted seedlings are covered with a transparent plastic film for heat preservation; the young buds growing on the stock are removed after the scion survives to prevent competition for nutrients with the scion; when the air temperature is higher than 25°C, the covering film is removed and a shading net is installed, and the grafted seedlings are watered to prevent high temperature from burning the grafted seedlings. Do not water the grafting interface when watering the grafted seedlings, and carry out normal water and fertilizer management.

[0104] Experiment: Nanchuan Paulownia grafting experiment

[0105] 1. In order to better compare, the healing accelerators prepared in Example 1 and Comparative Examples 1-7 are subjected to Nanchuan Paulownia grafting experiment, the experiment is carried out in the nursery of the national forest tree breeding base in Baomu, Longsheng Town, Qijiang District, Chongqing City, the experiment is divided into 9 groups: experimental group 1, control group 1-7, blank control group, and each group has 50 grafted seedlings, three times repeated.

[0106] 2. Selection of healing accelerator

[0107] Experimental group 1: the healing accelerator prepared in Example 1 is used; control group 1-7: the healing accelerators prepared in Comparative Examples 1-7 are used respectively; blank control group: water is used as the control.

[0108] 3. Nanchuan Paulownia grafting method

[0109] The experimental group 1, the control group 1-7 and the blank control group are all grafted with Nanchuan Artocarpus by the method of Example 4. Since the healing accelerator in the control group 7 does not contain guar gum and sodium tetraborate, it is in liquid form and is directly sprayed on the grafting interface, with the spraying amount being 1 / 2 of the weight of the healing accelerator in the experimental group 1.

[0110] 4. Healing condition detection of the grafting interface: the healing conditions of the grafting interface of the grafting seedlings in each group were observed at the 10th day and the 15th day after grafting, and the average data are shown in Table 1.

[0111] 5. Survival rate detection: the survival rate was observed 30 days after grafting, and the average data are shown in Table 2:

[0112] Table 1

[0113] 10-day incidence (%) 10-day healing rate (%) 15-day incidence (%) 15-day healing rate (%) Experiment group 1 6.67％ 50.67％ 8％ 60.67％ Control group 1 10％ 42％ 12％ 51.33％ Control group 2 9.33％ 42.67％ 10.67％ 52％ Control group 3 10％ 40.67％ 11.33％ 48.67％ Control group 4 20％ 42％ 22％ 50％ Control group 5 8.67％ 42.67％ 10％ 52％ Control group 6 10.67％ 43.33％ 12.67％ 52.67％ Control group 7 16％ 39.33％ 19.33％ 44.67％ Blank control 29.3％ 27.33％ 33.33％ 33.33％

[0114] Table 2

[0115]

[0116] According to the data analysis of Table 1, it can be known that:

[0117] (1) The morbidity of the control group 1-7 and the blank control group at the 10th day and the 15th day is higher than that of the experimental group 1. Among the control groups, the morbidity of the control group 4 at the 10th day and the 15th day is the highest, followed by the control group 7. This is because the healing accelerator in the control group 4 is not prepared with diallyl thiosulfoxylate, which cannot effectively kill the pathogenic bacteria, resulting in the infection of the grafting interface by the pathogenic bacteria and the increase of the morbidity. The healing accelerator in the control group 7 is not prepared into a gel form, which causes the healing accelerator to lose its effect quickly after use, and the subsequent inhibition of the pathogenic bacteria is reduced, resulting in the increase of the morbidity.

[0118] (2) Compared with the control group 1-7 and the blank control group, the healing rate of the experimental group 1 is increased. Among them, compared with the experimental group 1, the healing rates of the control group 1-2 at the 10th day are reduced by 8.67% and 8%, respectively, and the healing rates at the 15th day are reduced by 9.34% and 8.67%, respectively. This is because the healing accelerator in the control group 1 does not contain phenethylamine solution, and the healing accelerator in the control group 2 does not contain magnesium hydroxide, which causes the accumulation of harmful substances in the grafting interface due to the oxidation of the caffeic acid ethyl ester and the coumaric acid in Nanchuan Artocarpus, affecting the differentiation of the callus and reducing the healing rate.

[0119] (3) Compared with the experimental group 1, the healing rate of the control group 3 at the 10th day is reduced by 10%, and the healing rate at the 15th day is reduced by 12%. This is because the healing accelerator in the control group 3 does not contain cupric oxychloride, which cannot improve the activity of phenylalanine ammonia lyase, cannot promote the synthesis of lignin and the formation of tubular molecules, and inhibits the transportation of water and nutrients between the stock and the scion, resulting in the reduction of the healing rate.

[0120] (4) Compared with the experimental group 1, the healing rate of the control group 5 is reduced by 8% on the 10th day and by 8.67% on the 15th day, which is due to the fact that the healing accelerator in the control group 5 does not add 2-aminoethanesulfonic acid, and fails to reduce the oxidation of polyphenol oxidase on coffee acid ethyl ester, coumaric acid and other substances, resulting in the generation of harmful substances at the grafting interface, thereby reducing the healing rate;

[0121] (6) Compared with the experimental group 1, the healing rate of the control group 6 is reduced by 7.34% on the 10th day and by 8% on the 15th day, which is due to the fact that the healing accelerator in the control group 6 does not add charcoal powder, and fails to inhibit the accumulation of bleeding liquid at the grafting interface, thereby affecting the healing of the grafting interface;

[0122] (7) Compared with the experimental group 1, the healing rate of the control group 7 is reduced by 11.34% on the 10th day and by 16% on the 15th day, which is due to the fact that the healing accelerator in the control group 7 does not add guar gum and sodium tetraborate to prepare a gel, resulting in the quick loss of the healing accelerator and the reduction of the effect of the healing accelerator on the grafting interface, thereby reducing the healing rate. It is shown that the grafting interface coated with the healing accelerator prepared by the application can reduce the incidence rate, improve the healing rate, and reduce the influence of endogenous substances, nutrient transportation, pathogenic bacteria invasion and other adverse factors on the survival rate of grafting.

[0123] According to the data analysis of Table 2, it is shown that the survival rate of grafting of the control groups 1-7 and the blank control group is obviously lower than that of the experimental group 1, which indicates that the method of the application and the coating of the healing accelerator prepared by the application on the grafting interface can reduce the influence of various adverse factors on the survival rate of grafting, effectively improve the survival rate of grafting of Nan Chuan Mappia, and the survival rate of grafting can reach more than 60%.

[0124] The above examples are only used to illustrate the technical solutions of the application but not limit the application. Although the application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the application can be modified or replaced equivalently without departing from the purpose and scope of the application, and all of them should be covered in the scope of the claims of the application. The technical, shape and structure parts not described in detail in the application are well-known technologies.

Claims

1. A method of grafting of Alstonia scholaris, characterized in that, The grafting method is as follows: (1) selection and treatment of rootstock: selecting Nan Chuan Mappia seedlings as rootstock, and performing stump treatment on the rootstock; (2) selection and treatment of scion: selecting fruit-bearing mother trees of more than 8 years, collecting the current year fruit-bearing branches in the upper part of the crown as scions, and placing the scions with the cut end down into river sand for preservation; (3) grafting: grafting is performed from mid-January to early February, and the grafting method is selected as cutting grafting, and the grafting agent is coated on the grafting interface after grafting; (4) binding: the grafting is completed after the grafting interface coated with the grafting agent is tightly bound with the grafting film, and the grafted seedlings are obtained; (5) management after grafting: covering the grafted seedlings with film, removing the sprouts germinated on the rootstock after the scion survives, and performing normal water and fertilizer management; The grafting agent comprises the following raw materials by weight: 20-40 parts of phenethylamine, 20-40 parts of magnesium hydroxide, 20-30 parts of copper oxychloride, 1-2 parts of diallyl thiosulphinate, 20-30 parts of 2-aminoethanesulfonic acid, 80-100 parts of charcoal powder, 300-500 parts of guar gum, and 1-2 parts of sodium tetraborate.

2. The grafting method for *Muboluo* var. *nanchuanensis* according to claim 1, characterized in that, In step (1), the rootstock has a ground diameter of 0.5-1.2 cm and a seedling age of 1-2 years.

3. The grafting method for *Muboluo* var. *nanchuanensis* according to claim 2, characterized in that, In step (1), the rootstock is stumped within 2 cm from the ground.

4. The grafting method for *Muboluo* var. *nanchuanensis* according to claim 3, characterized in that, The scion has a thickness of 0.5-1.2 cm and a length of 15-20 cm.

5. The grafting method for *Muboluo* var. *nanchuanensis* according to claim 4, characterized in that, In step (2), the humidity of the river sand is 60-65%.

6. The grafting method for *Muboluo* var. *nanchuanensis* according to claim 5, characterized in that, In step (2), the top of the scion needs to be exposed to the sand surface by 5-10 cm when placed in the river sand.

7. The grafting method for *Muboluo* var. *nanchuanensis* according to claim 6, characterized in that, In step (2), the sand storage time of the scion is ≤10 days.

8. The grafting method for *Muboluo* var. *nanchuanensis* according to claim 7, characterized in that, The preparation method of the grafting agent is as follows: A: 20wt% phenethylamine solution is prepared by adding water to phenethylamine; B: water is added to diallyl thiosulphinate, mixed uniformly, and then subjected to water bath at 40-60℃, and after heating and stirring for 5-7 min, 2-aminoethanesulfonic acid is added and mixed uniformly, and then magnesium hydroxide is added and stirred uniformly to obtain a mixed solution; C: guar gum and sodium tetraborate are added to water and mixed uniformly, and then heated to 75-85℃, and stirred at constant temperature for 3-5 min, and then cooled to room temperature, and then charcoal powder, phenethylamine solution, mixed solution and copper oxychloride are added, and then placed in a homogenizer, and then homogenized at a speed of 500-700 r / min for 3-5 min to obtain the grafting agent.

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