69 results about "Fat transplantation" patented technology

The present invention provides a closed loop fat transplantation system newly designed to aspirate a fat, to handle it without receptacle transfer and to transplant it into the human body, which is composed of a fat transplantation receptacle(hereinafter referred as FT receptacle) and an exterior mechanical pressurization / decompression means. The said FT receptacle includes a vertical hem portion where joining selectively individual syringe needle or cannelae, a cylinder-shaped receptacle to aspirate and store fat, a piston head to parcel the said cylinder-shaped receptacle into front fat storing space and rear pressurization / decompression space. The said pressurization / decompression means can give positive / negative pressure to the fat storing space through the piston head of the said FT receptacle and it has a structure separable from the said FT receptacle. Accordingly fat transplantation can be performed without receptacle transfer using the same FT receptacle extracting fat from patient's body giving negative pressure through the piston head to the fat storing space.

Methods for lipomodeling by peripherally administering a modulator of a Y receptor are provided. Methods may comprise reduction of a fat depot by administering a Y receptor antagonist proximally and / or directly to the site of the fat depot. Other methods comprise increasing or stabilizing a fat depot or fat graft by administering a Y receptor agonist proximally and / or directly to the site of the fat depot or fat graft. Also provided are methods for stimulating wound healing by administering a Y receptor agonist proximally to a wound site.

A portable apparatus useful for collection and processing of human biological material containing adipose, such as extracted during a lipoplasty procedure to prepare a concentrated product (e.g., stromal vascular fraction) or a fat graft composition. The apparatus has a container with a containment volume with a tissue retention volume and a filtrate volume separated by a filter and with a tapered portion to a collection volume for collecting concentrate product. Inlet and suction ports provide access to the tissue retention volume and filtrate volume, respectively, and an extraction port provides versatile access for removal of target processed concentrate material or fat graft material, which access may be via a lumen through a rotatable mixer shaft. Access ports may be configured for access only from above the container. The apparatus may include a tissue collector disposed in the disuse retention volume to engage and collect collagen or other stringy tissue. A method of processing adipose tissue to concentrate leuko stromal vascular cells includes multi-step processing using a portable container.

The invention discloses an adipose transplantation material containing highly concentrated adipose-derived stem cells and an extracellular matrix (ECM), and a pure physical preparation method and an application thereof; with application of a fluid vortex principle, most of mature adipocytes in an adipose tissue are broken; at the same time, with application of a flocculation sedimentation technology, oil droplets generated from breaking the adipocytes are gathered and removed. A series of mechanical processing processes are adopted, the pure physical means is adopted, and no any exogenous substances are added. The multiple physical methods are used for removing the mature adipocytes and grease affecting the tissue retention rate after transplantation. In a prepared SVF-gel, the adipose-derived stem cells and endothelial progenitor cells are highly concentrated; the concentrated stromal vascular fraction cells (SVFs) still adhere on the ECM, the number of stem cells fix-planted in local after transplantation is far greater than the ordinary adipose transplantation number; as a stem cell therapy method, the adipose transplantation material has better long-term curative effect.

A portable apparatus useful for collection and processing of human biological material containing adipose, such as extracted during a lipoplasty procedure to prepare a concentrated product (e.g., stromal vascular fraction) or a fat graft composition. The apparatus has a container with a containment volume with a tissue retention volume and a filtrate volume separated by a filter and with a tapered portion to a collection volume for collecting concentrate product. Inlet and suction ports provide access to the tissue retention volume and filtrate volume, respectively, and an extraction port provides versatile access for removal of target processed concentrate material or fat graft material, which access may be via a lumen through a rotatable mixer shaft. Access ports may be configured for access only from above the container. The apparatus may include a tissue collector disposed in the disuse retention volume to engage and collect collagen or other stringy tissue. A method of processing adipose tissue to concentrate leuko stromal vascular cells includes multi-step processing using a portable container.

A syringe piston used in fat transplantation is structured such that free oil is easily separated from suctioned fat by a syringe piston having a filter and naturally discharged through the rear side thereof. The syringe piston without a shaft, used in fat transplantation, disposed in a syringe-shaped cylindrical vessel, includes a piston body without the shaft, a packing coupled with an outer surface of the piston body to seal between the piston body and the syringe-shaped cylindrical vessel, a free oil discharging hole communicated with the front side and the rear side of the piston body, an opening and closing device for opening and closing the free oil discharging hole, and a filtering device disposed in a passage through which free oil is discharged to filter fat and pass the free oil.

The invention belongs to the technical fields of tissue engineering and biological materials, in particular to a transplanting material of fat granule tissues compounded with SVFs (Stromal Vascular Fractions) and PRF (Platelet-Rich Fibrins) as well as a preparation method and application thereof. The transplanting material comprises fat granules, SVFs and PRF membrane granules. The preparation method comprises the following steps of: extracting fat granules from a patient by adopting liposuction, additionally extracting SVFs obtained by fat granule digestion from the same patient and extracting PRF membrane granules obtained by blood centrifuging from the same patient; mixing the SVFs with the PRF membrane granules; incubating at the temperature 37 DEG C for 10 minutes; and mixing the fatgranules, the SVFs and the PRF membrane granules to obtain the transplanting material of fat granule tissues compounded with the SVFs and the PRFs. The material can be applied to soft tissue molding and soft tissue defect repairing; by adopting the material, the absorption of transplanted fat tissues is effectively reduced, and the fat transplanting effect is improved.

The invention discloses a closed full-automatic fat transplantation device and a fat transplantation method thereof. The fat transplantation device comprises a liposuction needle tube, a lipochondrionseparation and purification cylinder and a fat injection device. The lipochondrion separation and purification cylinder is connected with the liposuction needle tube and the fat injection device through hoses separately; the liposuction needle tube comprises an outer tube, an inner tube, a liposuction handle, a water injection tube and a first motor, the lipochondrion separation and purificationcylinder comprises a cover body and a cylinder body, the cover body covers the upper portion of the cylinder body to form a full-closed fat storage barrel, the cover body is provided with a negative pressure interface and a fat suction interface, the fat injection device comprises an injector, a control box and a transmission device, the injector is clamped to the transmission device, and the control box is located on a shell of the transmission device. The problems that in the fat transplanting process, lipochondrion damage is large, infection is likely to be caused in the purification process, the operation intensity is large, and the blood vessel blockage risk exists are solved.

The invention belongs to the field of biomedicine, and particularly discloses a preparation method of an adipose tissue breast augmentation material enriched with autologous stem cells. In the present invention, only 5ml of tissue is extracted from the patient, and a large number of adipose stem cells are obtained through separation, purification, and in vitro culture and expansion, and finally added to the newly prepared SVF components, mixed with adipose tissue for breast augmentation transplantation. The invention can reduce the amount of adipose tissue used for preparing SVF during fat transplantation, so as to make up for the disadvantage that a large number of women cannot obtain a large amount of adipose tissue to prepare SVF due to low body mass index and cannot perform autologous fat breast augmentation.

The invention discloses an autologous fat filler and a preparation method thereof, wherein the autologous fat filler is constructed by simulating a tissue engineering method. The autologous fat filler comprises the following ingredients of a cytoskeleton, a cellular constituent, a growth factor and a scar resistant factor at the volume ratio of (109-115):(15-30):4:0.5. The effective ingredients are combined, the cytoskeleton part provides a skeleton made of a plant cell ingredient, an injectable gel is favorable for the adhesion of a cell, a washing fat occupies most of a filling volume, a stem cell and an SVF (Stromal Vascular Fraction) component are differentiated into an adipose cell and a vascular endothelial cell, and various excreted factors are good for maintaining a transplanting microenvironment and generating a blood vessel. According to the provided autologous fat filler and the preparation method thereof, the stability of the transplanting fat is effectively improved, the absorption of the transplanted fat is reduced, the untoward effect of fibrosis of a transplant is reduced, and the development of an autologous fat face filling plasty is promoted.

The invention discloses a preparation method and an application of a biological scaffold material and SVF assistant adipose tissue. Under an aseptic condition, the biological scaffold material, a stromal vascular fraction (SVF) prepared by digesting purified adipose granules with a medical-graded collagenase, and the purified adipose particles are evenly mixed according to a certain proportion to prepare an adipose graft for filling. The SVF is a mixture of adipose mesenchymal stem cells and various cells, is transplanted after being mixed with autogenous adipose particles, and can be continued to be developed into a mature adipose tissue in vivo; the biological scaffold material can provide a support for transplanted adipose, allows the transplanted tissue to be prone to revascularization, and improves the survival rate of the transplanted tissue; the transplanted tissue is suitable for being injected with a 16 G or 18 G needle, facilitates clinical application, and can be used for repairing of concave deformity (such as tempora and cheek concave), beautifying packing of faces and chests, soft tissue function reconstruction and the like; and especially in the beautifying packing, the prepared transplanted adipose tissue can promote shape recovery and prevent liquefactive necrosis.

The invention discloses a self-purified fat quantitative transplanting device and relates to the field of autologous fat transplantation plastic surgery instruments, which is in particular suitable for a little or quantitative fat transplantation during autologous fat transplantation plastic surgery and acts on separation of fat and liquid outside the fat and implantation of purified fat. According to the self-purified fat quantitative transplanting device disclosed by the invention, injector cylinders of different volumes are respectively and uniquely matched, and an injector cylinder piston head replaces an existing injector cylinder piston push rod so that the original changeless push rod is changed to be a channel with a pipe, the channel is provided with a filter screen with the certain aperture, a piston, a negative-pressure regulating hole and a negative-pressure pump connection pipe joint; a product is directly inserted into the injector cylinder, a negative-pressure pump provides negative pressure, a fat extracting needle is directly inserted into a fat-extracted part of a human body, fat enters the injector cylinder after leaving from the human body, the fat is left in the injector cylinder after a fat and water mixture in the injector cylinder passes through the filter screen under negative pressure, other liquids are separated from the fat after passing through a negative-pressure pipe connected with the negative-pressure pump connection pipe joint, and the purified fat is used for being directly transplanted to a required position; and therefore, purposes of saving time and labor and realizing safety and effectiveness are achieved.

The invention discloses a handheld automatic device used for sucking and injecting fat and a use method of the device. The handheld automatic device comprises a syringe, a piston, a shell and a driving assembly, wherein the piston is connected with the interior of the syringe in a sliding mode, and the driving assembly is arranged in the shell and drives the piston; the driving assembly comprisesa power device, a flexible long shaft, a round gear rotating disc and a motor, and the flexible long shaft is connected to the end, close to the tail portion of the syringe, of the piston; the long shaft is wound on and connected with the round gear rotating disc, and the motor drives the gear rotating disc; a handheld handle is arranged at the end, away from the syringe, of the shell; the handheld handle is provided with a forward switch, a backward switch and a propulsion switch, and the advancing switch, the retreating switch and the propulsion switch are connected to the motor. The syringeis integrally connected with the shell, a driving device drives the piston so that the piston can move in the syringe in the radial direction, automatic operation can be achieved when operators suckand inject the fat, therefore fat transplantation is more precise and safer, and medical accidents in a controllable range are reduced.

The invention relates to the application field of adipose-derived stem cells, and in particular to a method for improving fat filling effect of face and other parts by using adipose-derived stem cells(ADSCs). The method mainly comprises extraction of fat and extraction and concentration of ADSCs and adipose cells, wherein the extraction of fat can be performed by selecting upper arms, abdomen, inner sides and outer sides of thighs as supply areas and marking according to subjective requirements and self conditions of experimenters. By controlling in-vitro treatment time of the ADSCs and combining with ASCs assisted fat transplantation technology, the method improves survival rate of transplanted fat cells on face, effectively improves user satisfaction after facial filling, and reduces morbidity of fat transplantation complications and sequelae.

A syringe piston used in fat transplantation is structured such that free oil is easily separated from suctioned fat by a syringe piston having a filter and naturally discharged through the rear side thereof. The syringe piston without a shaft, used in fat transplantation, disposed in a syringe-shaped cylindrical vessel, includes a piston body without the shaft, a packing coupled with an outer surface of the piston body to seal between the piston body and the syringe-shaped cylindrical vessel, a free oil discharging hole communicated with the front side and the rear side of the piston body, an opening and closing device for opening and closing the free oil discharging hole, and a filtering device disposed in a passage through which free oil is discharged to filter fat and pass the free oil.

The invention belongs to the technical field of medical fat transplantation, and particularly relates to a face filling agent containing adipose-derived stem cells and a preparation method thereof, and the face filling agent comprises the following components in parts by weight: 20-180 parts of cross-linked glucan, 5-120 parts of adipose-derived stem cells, 1-50 parts of polymethyl methacrylate and 10-150 parts of normal saline for injection. The facial filling agent has the beneficial effects that the facial filling agent uses cross-linked glucan as a main filling material, so that the survival rate of adipose-derived stem cells can be ensured, adipogenic differentiation of the adipose-derived stem cells can be induced, the tissue compatibility is better, the maintenance time is longer, the effect is more natural, the postoperative reaction is slight, and the lasting filling effect can be achieved.

An external fine pellet fat preparation all-in-one machine comprises a fat collection tank 6 and a support 9, and further comprises a fat cutter, a negative pressure generator 94 and a negative pressure tank group 92 which are installed on the support, wherein the fat cutter is installed on the support 9 through a fixing frame 7, the negative pressure generator 94 is connected with the negative pressure tank group 92 through a pipeline, and the negative pressure tank group 92 is connected with a negative pressure connector 69 of the fat collection tank 6 through a pipeline. The external fine pellet fat preparation all-in-one machine solves the problems that in the fat transplantation process, fat suction wastes time and labor, content of ADSCs (adipose-derived stem cells) is low, fat is lack of extracellular matrix, pollution is easily caused in the purification process and the like.

The invention belongs to the technical filed of fat transplantation, and particularly relates to a fat graft rich in high activity fatty granule cells and adipose-derived stem cells and a preparationmethod and application of the fat graft. The method comprises the following steps of performing manual negative-pressure liposuction with an injector, performing centrifugation on obtained fat to obtain a fat layer, enabling the lower layer of the fat layer to be digested to prepare cell suspension, pushing the upper layer to obtain an intermediate layer for transplantation, and finally, uniformlymixing the cell suspension with the intermediate layer to obtain the fat graft rich in high activity fatty granule cells and adipose-derived stem cells. The fat graft has the advantages that comparedwith conventional SVF-gel, the fat graft prepared by the method is higher in yield, and contains massive high-activity granule adipocyte, SVF-cells and adipose-derived stem cells, the concentration of the cells is 5-10 times of that of normal fat tissues, the survival rate of the transplantation fat can achieve 70%, the transplantation fat is soft and uniform in texture, and the probability of adverse reactions of oily tumor masses, tumor tubercles and the like is notably reduced.

The invention relates to the technical field of fat transplantation, and particularly discloses an efficient and traceless localizer for autologous fat injection for breast enlargement. The localizercomprises a base; a base sliding groove of which the opening is arranged vertically upwards is arranged in the base; the base is provided with a slideable arc-shaped plate; the arc-shaped plate is provided with an arc-shaped plate sliding groove arranged in the length direction of the arc-shaped plate and of which the opening is located on the upper end face and the lower end face of the arc-shaped plate; a sliding block in clearance fit with the arc-shaped plate sliding groove is arranged in the arc-shaped plate sliding groove; the arc-shaped plate is provided with a fixing mechanism for fixing the sliding block; and the sliding block is provided with an injection through hole for a syringe needle to pass through. The localizer avoids that the end part of the needle shakes due to the factthat both arms of a medical worker feel limp and numb after long-time surgery to cause augmentation of an injection wound, and thereby, traumas and discomfort brought to a surgery recipient by fat transplantation breast augmentation surgery are reduced, the labor intensity of the medical worker during the fat transplantation breast augmentation surgery is also reduced, and the efficiency of the fat transplantation breast augmentation surgery is increased.

The invention discloses a low-temperature granular fat collecting device for fat transplantation. The device comprises two parts, namely, a cylindrical outer housing and a cylindrical inner housing, wherein a cold water chamber for storing cooling water is formed between the outer housing and the inner housing; a water feeding port and a water draining port are correspondingly formed in the upper part and the bottom part of the outer housing; the inner housing consists of an upper cover and a lower barrel body; the upper end surface of the upper cover is provided with an absorbing port which communicates with a negative pressure system, a fat inlet which is connected to a liposuction tube, and a fat extracting port which is connected to a fat extracting needle and is provided with a blocking plug. With the adoption of the device, cooling water can be separated from an operation performing table environment, and the cooling water can be stored in the cold water chamber formed between the outer housing and the inner housing; the granular fat obtained by a small-diameter liposuction tube in a sterile and low-negative-pressure condition and other conditions is stored in the sterile low-temperature environment, so that the granular fat can remain good activity, and as a result, the convenience of transplantation operation can be ensured, and the survival rate of fat tissue can be increased after the operation. The device has the characteristics of being easily clinically operated, easily cleaned and disinfected, and capable of being repeatedly used.

The invention belongs to the technical field of regenerative medicine and discloses a method for preparing an adipose-derived ECM (extracellular matrix) through built-in ultrasonic waves, which comprises steps of centrifugation, ultrasonic crushing, centrifugal collection and the like. According to the preparation method, most mature adipose cells in adipose tissue are crushed through ultrasonic waves, manure adipose cells and SVF cells are removed, the ECM is prepared, and too low ECM content and cell factor loss caused by complex operation are avoided effectively. According to the method, the ECM rich in cell factors is prepared without addition of exogenous chemical substances, the prepared ECM contains no exogenous substances, and adipose autotransplantation and effectiveness of the ECM as a biological filling material can be improved. The adipose-derived ECM material prepared with the method can be applied to autotransplantation stem cell therapy, reduces complications caused by adipose transplantation, increases the survival rate of transplanted adipose and has broad application prospects.

The invention relates to a composite cryopreservation solution and a preparation method and an application thereof. The composite cryopreservation solution is mainly composed of the following components: polyvinylpyrrolidone, trehalose, glycerin, a Rock inhibitor and a solvent. The composite cryopreservation solution is composed of polyvinylpyrrolidone, trehalose, glycerin, the Rock inhibitor andthe like as the main components without addition of dimethylsulfoxide, so the risk of dimethylsulfoxide cytotoxicity is eliminated, the composite cryopreservation solution can be used for long-term oreven permanent preservation of adipose stem cell gel in ultra-low temperature, so as to provide an active, safe and effective self filler for future fat transplantation. After 6 months of cryopreservation, the adipose stem cell gel cryopreserved with the compound cryopreservation solution is transplanted into an animal body, and after 3 months, the adipose stem cell gel still shows good retentionrate and tissue morphology, and no complication or adverse reaction appears.

The invention relates to the technical field of fat transplantation devices, and discloses a point-to-point directional fat transplantation device which comprises a storage box and a sealing cover, a partition plate is fixedly connected to the inner wall of the storage box, a supporting plate is movably mounted at the top of the partition plate, a placement hole is formed in the outer surface of a placement plate, and the sealing cover is fixedly connected to the inner wall of the storage box. A temperature control box is fixedly connected to one side face of the storage box, a display screen is arranged on the outer surface of the temperature control box, and a temperature sensor is arranged in the temperature control box. The storage box has the following advantages and effects that the display screen can see the temperature in the storage box in real time through the temperature sensor, when the temperature is too low, the temperature sensor recognizes the temperature and then enables the electric heating wire to start heating, after the heating box is heated, the temperature in the storage box can rise through the temperature guide plate, and after the temperature is reached, the temperature sensor controls the storage box to stop heating; the internal temperature of the storage box is always in a constant-temperature state, so that the activity of the fat is not influenced, and the fat is convenient to store.

The pharmaceutical composition comprises a component A and a component B. The component A is an adipose-derived stem cell population, the component B is granular fat, and the cell density in the adipose-derived stem cell population is 1 * 10 < 5 >-1 * 10 < 8 > / mL. The volume ratio of the component A to the component B is 1: (10-30). The invention further provides a therapeutic agent for treating scleroderma and application of the therapeutic agent. The pharmaceutical composition provided by the invention is safe, effective, low in cost and good in rejection reaction effect, and a therapeutic agent containing the pharmaceutical composition is effective and safe in the aspect of improving graft retention of facial atrophy of LoS patients and is superior to simple fat transplantation or SVF-assisted fat transplantation. In addition, the therapeutic agent provided by the invention can more effectively improve the fat survival rate under the condition that blood perfusion is not obviously changed.

The invention discloses a fat purification method beneficial to fat transplantation. The fat purification method comprises the following steps: S1, adding a proper amount of extracted fat tissue fluidinto an inner tank from a feeding hole; S2, turning on a motor to enable the inner tank to rotate, and carrying out centrifugal treatment on the fat tissue fluid; S3, discharging bottom layer water through water outlet; and S4, sucking middle layer purified fat through a suction tube to obtain high-purity fat. Fiber tissue blocks and large blood clots are collected and removed through a comb plate arranged at the bottom of the inner tank, concentration removing is realized, the structure is simple, operation is convenient, fat purification time is shortened, and purification efficiency is improved.

The invention belongs to the technical field of regenerative medicine and discloses a preparation method for efficiently enriching a SVF (stromal vascular fraction) cell material through built-in ultrasonic waves, which comprises steps of centrifugation, ultrasonic crushing, centrifugal collection and the like. According to the preparation method, most mature adipose cells in adipose tissue are crushed through ultrasonic waves, and meanwhile, oil drops produced by crushed mature adipose cells are collected with a floccus centrifugal precipitation technology, so that the effect of enriching SVFs is realized. After ultrasonic treatment, the cell viability is substantially improved and the cell apoptosis rate is substantially decreased, so that the transplanting effectiveness is greatly enhanced. The cell material prepared with the method can be applied to autotransplantation stem cell therapy, reduces complications caused by adipose transplantation, increases the survival rate of transplanted adipose and has broad application prospects.

The invention relates to the technical field of fat transplantation, and in particular to a high-precision pressure propeller for autologous fat. The high-precision pressure propeller comprises a needle tube and a mounting seat; a screw rod is arranged on the mounting seat; a driving mechanism for driving the screw rod to vertically move downwards is arranged in the mounting seat; the driving mechanism comprises a gear; a first rack meshed with the gear is arranged in the mounting seat; a first rotating shaft is arranged in the mounting seat; a first bevel gear is arranged on the first rotating shaft; a screw rod is also arranged in the mounting seat; a second bevel gear matched with the first bevel gear is arranged on the screw rod; a slidable sliding block is also arranged on the screw rod; a rack bump is arranged at the lower end part of the first rack; and a support rod matched with the rack bump is connected with the sliding block. According to the invention, a poor fat transplantation effect caused by insufficient fat injection amount is avoided, and fat death caused by overlarge fat injection amount is avoided, so that the fat injection effect is improved.

The present invention provides a closed loop fat transplantation system newly designed to aspirate a fat, to handle it without receptacle transfer and to transplant it into the human body, which is composed of a fat transplantation receptacle (hereinafter referred as FT receptacle) and an exterior mechanical pressurization and decompression means. The said FT receptacle includes a vertical hem portion where joining selectively individual syringe needle or cannelae, a cylinder-shaped receptacle into front fat storing space and rear pressurization and decompression space. The said pressurization and decompression means can give positive / negative pressure to the fat storing space through the piston head of the said FT receptacle and it has a structure separable from the said FT receptacle. Accordingly, fat transplantation can be performed without receptacle transfer using the same FT receptacle extracting fat from patient's body giving negative pressure through the piston head to the fat storing space.