2 results about "Fat transplantation" patented technology

The application relates to the field of biological medicine, and particularly relates to a cold storage solution and application of a combined traditional Chinese medicine compound biological stent drug delivery system in eardrum repair. The cold storage solution comprises a basic solution and L-alanyl-L-glutamine; the basic solution comprises a STEEN solution. The cold storage solution can promote metabolism and synthesis of glutathione of fat grafts, regulate ROS mitochondrial oxidative stress, reduce cold storage damage of the fat grafts, and improve the cold storage quality of the fat grafts. Meanwhile, the application uses Shengji Yuhong collagen sponge which contains an important classical prescription for promoting repair, forms a functional biological material with supporting and pro-angiogenic effects, forms a linkage mode of "double-stage regulation before and after transplantation", closely combines the actual difficulties of cell damage of fat groups and reduction of repair success rate caused by long eardrum repair time in the clinic, explores a brand-new research mode for improving the eardrum repair scheme, and has far-reaching clinical significance.

This invention discloses a method for preparing high-viability fat grafts, comprising: aseptically transferring adipose tissue in a closed system while maintaining complete isolation from air during the transfer; performing multi-stage liquid washing on the adipose tissue, including an initial washing and a secondary washing, to remove blood components, tissue debris, and drug residues; subjecting the washed adipose tissue to gradient centrifugation, including an initial low-speed centrifugation and a secondary high-speed centrifugation, to achieve uniform separation of fat cells through a sieve; and collecting the centrifuged fat grafts to obtain a final product with high cell viability and a high proportion of concentrated stem cells. By combining multi-stage liquid washing with gradient centrifugation, efficient purification and uniform separation of fat cells are achieved, effectively reducing the risk of inflammation and removing harmful residues such as lidocaine, resulting in a final fat graft with a high proportion of stem cells and excellent biocompatibility, supporting regenerative medicine applications.