50 results about "Biogenic substance" patented technology

A bio-substance analysis method using a sensing substrate having a fluid channel is disclosed. The method includes mixing retroreflective particles with a detection solution containing a target bio-substance, wherein a first bio-recognition substance selectively reacting with the target bio-substance is modified on the retroreflective particles; placing the sensing substrate so that a bottom is located under a cover in a direction of gravity; injecting the detection solution containing therein the retroreflective particles into a fluid channel and maintaining the solution in the channel for a first time duration; turning the sensing substrate upside down so that the bottom is located above the cover in the direction of gravity and maintaining the sensing substrate in the turned state for a second time duration; irradiating light into the fluid channel through the bottom; and generating and analyzing an image based on light retroreflected from the retroreflective particles.

The present disclosure relates to a method of manipulating magnetic beads in a liquid inside a conduit, wherein the method comprises generating a variable magnetic field using at least one magnet or electromagnet to mix the magnetic beads in the liquid; and wherein a biological or chemical species is attached to the surface of the magnetic beads.

A biological measurement device (10) comprises a stimulation device (12) comprising at least one stimulation electrode (102a, 102b) and a stimulation circuit. The stimulation circuit is configured to provide an electrical stimulation signal to the at least one stimulation electrode (102a, 102b) such that the at least one stimulation electrode (102a, 102b) generates a predetermined electrodynamic force field applied to the biological substance. The sensing device comprises at least one sensing electrode (202a, 202b) and a sensing circuit, the at least one sensing electrode (202a, 202b) being configured to sense the responsive electrodynamic force field and to provide a corresponding electrical response signal to the sensing circuit. The electrical stimulation signal includes a plurality of different pseudo-random binary sequences, each pseudo-random binary sequence having a length and a data rate that provide a predetermined electrodynamic force field having a frequency range that is different from those provided by the other pseudo-random binary sequences.

A system comprises a source of enzymatic detergent and a cleaning device comprising an elongate member configured for advancement through a lumen within a medical device and at least one cleaning member coupled to a portion of the elongate member. The cleaning member comprises distal and proximal end portions and a central portion between the distal and proximal end portions. The central portion is shaped to create a pressure gradient along the central portion from the distal end portion to the proximal end portion. The combination of the enzymatic detergent and the unique nature of the cleaning device is sufficient to remove pre-existing biomatter from the surfaces of the medical device, such as biomatter that has already formed into a biofilm. The detergent loosens the protective layer of polysaccharides and proteins of the biofilm and the cleaning device then removes both the protective layer and the underlying biomatter.

The present invention relates to methods and conjugates to improve the penetrability of biological substances into the tumour microenvironment (TME) for therapeutic purposes, suitably the agent comprises at least: i a therapeutic domain; ii. a cleavable domain; and iii. a stabilisation domain.

The invention discloses a method for analyzing a polymeric lignin structural unit in watermelon peel, and belongs to the technical field of biological substance composition determination methods, the method comprises the following steps: S1, protective pretreatment: mixing a watermelon peel sample with a protective solution containing triphenylmethyl mercaptan; s2, thioacidolysis: adding a reaction solution containing boron trifluoride and ethanethiol, and heating for depolymerization; s3, in-situ neutralization and derivatization: adding a buffer solution, extracting and drying to obtain an organic phase, and then adding a silanization reagent for derivatization reaction; s4, qualitative and quantitative analysis; through the multi-level design of structure protection, efficient derivation and system integration, the established analysis method shows remarkable advantages in the aspects of inhibiting condensation reaction, improving operation safety, shortening analysis period and realizing quantification, effectively overcomes the technical bottlenecks of low recovery rate, high toxicity, long time consumption, incomplete detection and the like in the prior art, and has wide application prospects. And an excellent technical scheme is provided for composition analysis of the plant polymerized lignin.

The utility model discloses a novel biological experiment chromatography cabinet which comprises a chromatography cabinet main body, an upper air curtain unit, a lower air curtain unit, an internal and external circulation cooling and dehumidifying system and an embedded control panel. Wherein the upper and lower air curtain unit can automatically start an upper air curtain / upper and lower air curtains according to the condition of closing / opening a chromatography cabinet door, and the air volume of the air curtains can also be adjusted; the internal and external circulation cooling and dehumidification system can keep the humidity in the chromatography cabinet not to exceed a set value all the time. Therefore, the service life of the chromatography cabinet and the service life of chromatography equipment in the chromatography cabinet can be effectively prolonged, the safety of experiment operators is guaranteed, and the effects of saving energy and reducing cost are achieved. Meanwhile, due to the design that the control panel is embedded in the glass cabinet door of the chromatography cabinet, the whole chromatography cabinet looks more attractive and elegant and is rich in modern atmosphere. The low-temperature refrigerator not only can provide low-temperature conditions for various chromatographic devices to prepare protein / peptide / carbohydrate substances, but also can be used as a constant-temperature refrigerator for storing various biological substances at low temperature.

The present technique pertains to a method of preparation of a succinylated collagen-fibrinogen hydrogel and a succinylated collagen-fibrinogen hydrogel prepared thereby. The method of preparation of a succinylated collagen-fibrinogen hydrogel according to the present technique can produce a collagen-based bio-substance in a simple process, wherein collagen can be prevented from being entangled and an improvement can be brought about in hydrophilicity, cell growth rate, cell compatibility, and cell diffusion capability, whereby the collagen-based bio-substance exhibits excellent biocompatibility in vivo.

A method for recovering a biological substance and a device for recovering a biological substance capable of improving a recovery rate of a target biological substance with a simple configuration are provided. The method for recovering a biological substance uses an electrophoresis device having a gel 13 and recovery holes 11 and 111. The method for recovering a biological substance includes a step of starting application of a voltage for moving a target biological substance toward the recovery hole, after that, a step of removing buffer solution in the recovery hole before the target biological substance reaches the recovery hole, after that, a step of injecting a solvent into the recovery hole, and, after that, a step of recovering the target biological substance that reaches the recovery hole.

The invention relates to the technical field of biological substance preparation. The invention provides a catechin-phospholipid complex and an emulsion prepared from the catechin-phospholipid complex and capable of improving DHA (docosahexaenoic acid) and improving memory activity. The invention also provides an application of the catechin-phospholipid complex or the emulsion for improving the memory activity of DHA in preparation of health-care products. The emulsion prepared by adopting the catechin-phospholipid compound as an emulsifier can efficiently and synergistically entrap, stably protect and deliver the beta-carotene and the DHA in a targeted manner, so that the bioavailability and the multi-component synergistic effect of the beta-carotene and the DHA are enhanced, and the effect of improving the memory function is realized; meanwhile, good physical and chemical stability and digestion tolerance are also shown.

The present disclosure provides systems and devices for preserving unfrozen biological matter below its equilibrium melting temperature by applying pressure generated by isochoric freezing. The systems and devices include an isochoric chamber segmented into a first volume containing an aqueous solution with a first nucleation temperature and a second volume containing an aqueous solution with a second nucleation temperature, where the second volume increases when cooled below the second nucleation temperature, and also include a boundary separating the two volumes that transmits heat and pressure but not mass. Methods of using the systems and devices for preserving a biological matter without freezing at temperatures below its equilibrium melting point are also provided.

The utility model provides a kind of cell culture reaction kettle, it is related to bioreactor technical field, the cell culture reaction kettle, including servo motor, motor shaft, integrated shaft and tank body assembly, the motor shaft is connected to servo motor by pin, and is fixed by motor flange and base, motor shaft is connected with tank body assembly by self-locking device, and drives tank body assembly rotation, integrated shaft is arranged in the inside of tank body assembly, and not with tank body assembly rotation.The utility model is driven to realize stirring by servo motor, avoids the leakage risk brought by traditional magnetic stirring and mechanical stirring, when stirring kettle rotates, through the wave shape protrusion of stirring kettle bottom to make reactant mix, avoids the shear force of ordinary stirring to biological substance.

An electrophoresis apparatus includes a separation medium for electrophoretically separating a biological substance, a buffer solution tank containing a buffer solution, a recovery chamber for recovering a target biological substance, a separation wall separating the buffer solution tank and the recovery chamber, the separation wall controlling a flow of a buffer solution between the buffer solution tank and the recovery chamber according to a water level of the buffer solution, a position detector that detects a position of the target biological substance that is electrophoresed in the separation medium, and a water level control unit that controls the water level of the buffer solution in the buffer solution tank according to the position of the target biological substance.

This invention provides the following compounds and labeled biological substances having the compounds. 1 ~R 6 R 11 ~R 13 R 22 ~R 29 This represents a specific group, where n is an integer from 1 to 3. (Selected from R...) 1 R 2 R 5 R 22 ~R 25 One of them and selected from R 3 R 4 R 6 R 26 ~R 29 One of them is bonded via a linking group LL. The linking group LL represents a linking group with 1 to 100 atoms that does not possess any of the following: an aromatic hydrocarbon ring, a sulfonyl group, or a phosphonoyl group. R 1 ~R 6 R 22 ~R 29 At least one of them contains -(CH2-CH2-O) m - indicates a structure where m = 1 to 50. The following compounds are neutral compounds containing at least one carboxyl group or a substituent capable of bonding with biological material.

This invention provides a compound of formula (1) below, and a labeled biological substance having the compound. (Z-ring) 1 and ring Z 2 This indicates a 6-membered ring formed by cyclic atoms selected from carbon and nitrogen atoms. R 1 ~R 4 R 11 ~R 13 L 1 L 2 These represent specific functional groups, and n, α1, and α2 represent specific numbers. R 11 ~R 13 At least one of the groups contains a carboxyl group or a substituent capable of bonding with biological material. Compounds represented by formula (1) have at least one substituent consisting of -(CH2-CH2-O). m -R 21 The structure represented. R 21 The radical represents a specific group, and m represents a specific number. The compound represented by formula (1) is a neutral compound.

A method for reducing probability of ice nucleation during preservation of biological matter in isochoric systems by placing biological matter in a flexible, impermeable inner container, adding an inner solution with a melting point that is higher than a desired storage temperature, removing bulk gas from and sealing the inner container, placing the inner container in a rigid, non-thermally insulating outer container, filling the space between the inner and outer containers with an outer solution, removing bulk gas from and sealing the outer container, cooling the system to the desired storage temperature, maintaining the desired storage temperature for a desired storage period, warming the system to a temperature that is higher than the desired storage temperature, unsealing the outer and inner containers, and removing the biological matter. The outer solution has a melting point that is lower than the equilibrium melting point of the biological matter and the inner solution.

Devices, systems, and methods herein relate to controlling a temperature (e.g., thawing) of a low volume biological substance. Devices may include a container configured to receive a biological substance and to be used with a temperature control device to control a temperature of the biological substance. In some variations, the container includes a top portion, a body portion, and a bottom portion. The body portion may include a fluid reservoir to receive the biological substance. The bottom portion may include a temperature sensor configured to measure the temperature of the biological substance received in the fluid reservoir. In some variations, the top portion and the bottom portion may couple to the body portion via connectors. Systems may include the container and the temperature control device configured to receive the container and control the temperature of the biological substance.

This invention relates to a method for evaluating the spectra of biological material of animal, plant, or mixture thereof, the method comprising the steps of: a) detecting spectrometers in a network formed by at least one spectrometer and an input / output device; b) requesting the respective status of each spectrometer in the network of step a), and displaying the detected spectrometers and their status on the input / output device, wherein the status reflects whether the spectrometer is available for recording spectra; c) receiving a selection from spectrometers available for recording spectra on the input / output device; d) recording the spectrum of the sample material of animal, plant, or mixture thereof on the spectrometer selected in step c); e) predicting at least one parameter from the spectrum of step d) using at least one calibration function and / or calibration plot suitable for predicting the value of the parameter. The values ​​of the parameters, wherein the at least one parameter is selected from the content of at least one amino acid, crude protein content, ammonia content, content of all amino acids containing ammonia, content of all amino acids not containing ammonia, crude fat content, dry matter content, crude ash content, energy content, content of at least one biogenic amine, content of at least one anti-nutritional factor, content of at least one sugar, starch content, crude fiber content, neutral detergent fiber content, acid detergent fiber content, total phosphorus content, phytic acid phosphorus content, reactive lysine content, total lysine content, ratio of reactive lysine content to total lysine content, protein dispersibility index, protein solubility, trypsin inhibitor activity, urease activity, and processing condition index (PCI); and f) displaying the predicted results of step e) on the input / output device.

The invention relates to the technical field of biomass granularity recognition, in particular to a biomass granularity recognition method and device.The method comprises the steps that geometrical characteristic parameters of biomass are obtained according to a substance image of the biomass so as to determine the sphericity degree and the length-width ratio of the biomass; determining the category of the biomass according to the sphericity and the length-width ratio, obtaining the corresponding burn-out time according to the category, and determining the range of the burn-out time; and in response to the situation that the range is the first range, determining a diameter correction value of the biomass according to the length-width ratio and the sphericity degree, and identifying the granularity distribution of the biomass according to the diameter correction value to obtain a biomass particle evaluation result. Therefore, the problems that in the related technology, due to the fact that different equivalent diameters are adopted for defining the biomass particles, the measurement result lacks comparability and universality, and then popularization and application of an image analysis method in industrial scenes are restricted are solved.

The present invention relates to methods and conjugates to improve the penetrability of biological substances into the tumour microenvironment (TME) for therapeutic purposes, suitably the antibody-drug-conjugate comprises an engineered agent conjugated to one or more payloads, wherein the agent comprises: i a therapeutic domain; ii. a cleavable domain; and iii. a stabilisation domain. The cleavable domain is positioned between the therapeutic domain and the stabilising domain.

The subject of the invention is a microscope for high-resolution and specific analysis of biological substances.The microscope according to the invention is characterized by a power supply unit capable of generating constant current attached to the microscope housing; a set of mirrors and illuminating optics; a collimator placed between a monochromatic light source emitting light in a very narrow range of wavelengths and the collector lens; a dark-field condenser and objective including an in-between space to accommodate the stained biological specimen; and a surveillance camera or eyepiece, and a removable color filter placed between the objective and the surveillance camera or the eyepiece.The invention also covers the method to analyze biological specimens with fluorescent staining, wherein the biological contents of the specimen are analyzed in a growing medium in liquid state after the addition of antibodies marked with fluorescent stain, using the microscope according to the invention.The microscope according to the invention enables up to one order of magnitude higher resolution than conventional light microscopes and 10-30% higher resolution compared to currently used dark-field microscopes and provides a remarkably sharper camera image.

The present disclosure relates to apparatus for extracting a chemical or biological species from a sample, comprising: a first liquid in a first region; a second liquid in a second region, wherein the

The invention discloses an in-situ photocatalytic oxidation effect evaluation method and system for VOCs discharged by plants, and relates to the technical field of biological substance analys.The method comprises the steps that living branches are placed into a first dynamic flux box and a second dynamic flux box respectively, and gaps of penetrating-out parts are sealed; environment air is introduced, the air inlet flow is set, continuous air exchange is maintained, and air in the box is evenly mixed; a to-be-detected photocatalyst is applied to leaf surfaces of the first flux box, and the catalyst is not applied to the second flux box as a contrast; sampling is switched under illumination, and the target VOCs concentration is obtained; firstly, baseline flux is measured, a natural difference coefficient is obtained through calculation, and after contrast flux is corrected, the VOCs removal efficiency is calculated. According to the technical scheme, the background concentration and the outlet concentration are synchronously obtained under the same sampling gas path, fluctuation and individual difference interference are reduced, in-situ quantitative evaluation is achieved, and result accuracy, comparability and repeatability are improved.

A shape memory polymer that provides a color-based visual cue for shape change. The shape memory polymer can be used for sensing, where a material is designed to change shape in the presence of a specific reagent, whether chemical (such as a particular acid or reactive oxygen species) or biological (such as a particular bacterial or mammalian enzyme). The shape change coupled with the color change will enhance surveillance of the presence of these reagents and would be particularly useful when used in a wound dressing to detect the presence of a bacterial infection. Materials formed from the shape memory polymer may also be used to sense small-scale deformations of surfaces by cells or mechanical forces.

The present invention relates to methods and conjugates to improve the penetrability of biological substances into the tumour microenvironment (TME) for therapeutic purposes, suitably the antibody-drug-conjugate comprises an engineered agent conjugated to one or more payloads, wherein the agent comprises: i a therapeutic domain; ii. a cleavable domain; and iii. a stabilisation domain. The cleavable domain is positioned between the therapeutic domain and the stabilising domain.

This invention discloses an analytical method for polymeric lignin structural units in watermelon rind, belonging to the technical field of biomaterial composition determination methods. The method includes the following steps: S1, protective pretreatment: mixing the watermelon rind sample with a protective solution containing triphenylmethanethiol; S2, thioacid hydrolysis: adding a reaction solution containing boron trifluoride and ethanethiol, followed by heating to depolymerize; S3, in-situ neutralization and derivatization: adding a buffer solution, extracting and drying to obtain the organic phase, then adding a silanizing reagent for derivatization; S4, qualitative and quantitative analysis. This invention, through a multi-level design of structure protection, efficient derivatization, and system integration, establishes an analytical method that exhibits significant advantages in inhibiting condensation reactions, improving operational safety, shortening the analysis cycle, and achieving quantification. It effectively overcomes the technical bottlenecks of existing technologies, such as low recovery rate, high toxicity, long processing time, and incomplete detection, providing an excellent technical solution for the analysis of the composition of plant polymeric lignin.

A novel method for easily determining the behavior of a nematode includes: dropping a liquid containing a biological substance of a subject into a container in which a nematode is movably stored; measuring, as a reference amount, an amount related to light in a measurement range in the container irradiated with light before or immediately after the dropping of the liquid; measuring, as a determination amount, an amount related to light in the measurement range in the container irradiated with the light after an observation time provided for movement of the nematode elapses from the dropping of the liquid; and determining whether or not the determination amount has decreased by comparison with the reference amount.

The invention describes a method for freeze-drying biological matter (100), the method comprising the steps of introducing a fluid biological matter (100) into a flexible receptacle (200), closing the flexible receptacle (200), freezing the fluid biological matter (100), thereby obtaining a frozen biological matter (100), placing the flexible receptacle (200) in a freeze dryer, lowering the pressure in the freeze dryer to a pressure lower than the vapor pressure of water, wherein the flexible receptacle (200) remains sealed at least until it inflates, freeze drying the frozen biological matter (100) by providing heat to the frozen biological matter (100) such that a vapor pressure at the frozen biological matter (100) is larger than the vapor pressure at one or more cooling element(s) (260, 600) in gas communication with the frozen biological matter (100), thereby obtaining a freeze- dried biological matter (100) in the first portion (210) and frozen water at the one or more cooling element(s) (260, 600), and sealing the first portion (210) off from a second portion (220) of the flexible receptacle (200) not containing the freeze-dried biological matter (100).

The present disclosure relates to apparatus for extracting a chemical or biological species from a sample, the apparatus comprising: a first liquid in a first region; a second liquid in a second region, wherein the second liquid is immiscible with the first liquid; and beads for transporting the chemical or biological species; wherein the apparatus is configurable between: a first configuration in which there is a path for transport of the beads from the first liquid in first region to the second liquid in the second region, and a second configuration in which a barrier is provided between the first region and the second region.