57 results about "Neutral Detergent Fiber" patented technology

The invention belongs to the technical field of feed processing, and particularly relates to a wheat straw fermented feed and a preparation method thereof. In order to solve the problems that in the prior art, wheat straw is utilized for biological fermentation to prepare fermented feed, the process is complex, and the fermentation effect needs to be improved, the invention provides the preparation method of the wheat straw fermented feed, the method comprises the following steps: crushing wheat straw, mixing the crushed wheat straw with corn flour, adding a compound bacteria solution, and carrying out anaerobic fermentation in a fermentation container for 10-30 days to prepare the wheat straw fermented feed. According to the method disclosed by the invention, the palatability of the wheat straw is improved by adopting a mode of mixing the wheat straw and the corn flour and combining with the special fermentation compound bacteria liquid, the nutritional value is increased, the content of crude protein is increased by 40% or above compared with that of raw materials, the content of neutral detergent fibers and acid detergent fibers is reduced by 16-19% respectively, and the pH value is reduced by 30%. The raw materials are easy to obtain, the fermentation method is simple and convenient, the fermentation effect is good, and good practical value is achieved.

The invention discloses a processing method and application of edible fungus chaff. The processing method comprises the following steps: crushing the edible fungus chaff to obtain fungus chaff particles; carrying out extrusion puffing treatment on the mushroom bran particles by adopting a twin-screw extruder to obtain a puffed material; drying and crushing the puffed material to obtain puffed mushroom bran powder; mixing puffed mushroom bran powder, bean dregs and cellulase to serve as a fermentation substrate, and adding the mixed bacterial liquid into the fermentation substrate to form fermentation mixed liquid; the mixed bacterial liquid is formed by mixing a candida utilis bacterial liquid, a lactobacillus plantarum bacterial liquid and a bacillus subtilis bacterial liquid; and fermenting the fermentation mixed solution under a ventilation condition to obtain a final edible mushroom bran product. Compared with a traditional processing method, the content of crude protein, the content of acid-soluble protein and the protein dispersion index of the edible fungus chaff processed through the processing method are all remarkably improved, and the content of crude fiber, neutral detergent fiber and acid detergent fiber is all remarkably reduced.

The invention discloses an alfalfa growth period and quality related protein MsCOG1 as well as a coding gene and application thereof. The Dof transcription factor is separated from medicago sativa, the amino acid sequence of the Dof transcription factor is as shown in SEQ ID No.2, and the nucleotide sequence of the coding gene of the Dof transcription factor is as shown in SEQ ID No.1. According to the invention, the coding gene of the Dof transcription factor in medicago sativa is over-expressed in medicago sativa, so that compared with a wild type, the obtained transgenic medicago sativa material has the advantages that the flowering time is obviously delayed, the contents of nutritional ingredients such as crude protein and trace elements are obviously increased, and the contents of acid detergent fibers, neutral detergent fibers and lignin are obviously reduced; the relative feeding value and the relative feeding quality are obviously improved. The invention provides a gene resource for molecular breeding and genetic engineering improvement of forage grass, and has application prospects in the aspects of cultivating high-quality and high-yield forage grass varieties or controlling the flowering period of the forage grass, improving the quality of the forage grass and the like.

The invention discloses a prediction model for predicting the utilization rate of standardized mineral elements of wheat feed raw materials on white feather broiler chickens and construction and application of the prediction model, conventional nutritional ingredients and antinutritional factor contents are obtained as input variables, measured values are taken as output variables, and the standard phosphorus utilization rate = 67.54-2.60 * neutral detergent fibers is obtained; the standardized copper utilization rate is equal to 13.00 + 2.03 * crude protein-3.89 * crude fiber + 13.85 * crude ash-2.51 * phytic acid, the standardized iron utilization rate is equal to 10.05 + 3.21 * crude protein-12.16 * crude fiber + 16.68 * crude ash, and the standardized zinc utilization rate is equal to 14.12 + 29.51 * crude protein-5.42 * crude fiber. The method solves the problems of large workload and long time consumption in the existing determination of the standardized mineral element utilization rate of the wheat feed raw material, and can rapidly predict the standardized mineral element utilization rate of the wheat feed raw material on broiler chickens.

The invention provides a preparation method of a compound microbial agent and a pasture fermented feed, and belongs to the technical field of feed fermentation. The complex microbial inoculant disclosed by the invention comprises lactobacillus acidophilus, bacillus siamensis, bacillus amyloliquefaciens and pichia kluyveri. The lactobacillus acidophilus can produce acid and resist acid, and has better antibacterial performance; the bacillus siamensis can degrade lignin and reduce the content of neutral detergent fibers and acid detergent fibers in the fermented feed; the bacillus amyloliquefaciens produces cellulase and amylase with higher activity; the pichia kluyveri can increase the content of crude protein in the fermented feed and improve the nutritional performance of the feed. The four strains have a synergistic effect and jointly ferment forage feed, infectious microbes can be effectively inhibited, the fermented forage feed has excellent color, smell and texture, the palatability of the feed is improved, the appetite of animals is stimulated, and a good growth promoting effect is achieved; the fermented forage feed disclosed by the invention is high in nutritional quality and relatively high in feed utilization rate.

The invention relates to the technical field of plant genetic engineering, in particular to application of an algae NySAT2 gene in regulation and control of alfalfa crude protein and detergent fiber. According to the invention, a sequence after codon optimization of an NySAT2 gene coding region is obtained by using gene synthesis and codon optimization technologies, an algae NySAT2 gene overexpression vector is constructed, a target gene is introduced into an alfalfa genome by using an agrobacterium rhizogenes mediated genetic transformation method, and a transgenic plant is obtained by using a genetic transformation system. Molecular identification and phenotypic analysis show that on the premise of keeping normal growth vigor (plant height and biomass have no significant difference from those of a wild type), the crude protein content of a medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased compared with that of the wild type medicago sativa plant, and meanwhile, the contents of acid detergent fibers and neutral detergent fibers are remarkably reduced, so that the content of the crude protein in the medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased. The forage quality and the feeding value of the medicago sativa are effectively improved.

The application provides a pediococcus acidilactici M4 for low-moisture fermented feed and an application thereof, and belongs to the field of microbial application and feed technology. The application uses the pediococcus acidilactici M4 to prepare fermented total mixed ration. The bacteria have the characteristics of high osmosis and fast acid production, and can reduce the pH value of the fermentation system in a short time; have strong broad-spectrum bacteriostatic effect on escherichia coli, chicken white dysentery salmonella and staphylococcus aureus; and perfectly adapt to the fermentation of low-moisture total mixed ration. The strain is used as the fermentation strain of low-moisture total mixed ration, can significantly reduce the content of acid detergent fiber and neutral detergent fiber, improve the crude protein level, and then improve the digestibility of the daily ration, increase the content of probiotics such as lactic acid bacteria and yeast in the daily ration, reduce the content of pathogenic bacteria such as escherichia coli in the daily ration, and also can improve the content of volatile fatty acid in the daily ration, improve the quality of the daily ration, and improve the feeding value of the daily ration.

The application discloses a preparation method of bio-modified wheat bran containing xylo-oligosaccharide, and comprises the following steps: fermenting wheat bran by using mixed strains and composite enzymes; wherein the mixed strains comprise lactic acid bacteria, Saccharomyces cerevisiae and Bacillus licheniformis; and the composite enzymes comprise cellulase and xylanase. The preparation method provided by the application effectively reduces the contents of crude fiber and neutral detergent fiber in the wheat bran, increases the contents of crude protein and xylo-oligosaccharide in the fermented wheat bran, which are beneficial to the growth and development of animals, and improves the nutritional value of the wheat bran, so that the proportion of the wheat bran in feed application can be increased, and the addition amount of corn and soybean meal can be reduced.

The invention provides application of bacillus altitudinis H18 and a glucoside hydrolase gene thereof in improving rumen degradation of rice straws, and belongs to the technical field of straw reutilization. The bacillus altitudinis H18 is separated from goat rumen fluid, the bacillus altitudinis H18 can promote degradation of rice straw cellulose, and reference is provided for feed application of rice straw. Sequencing analysis finds that a whole genome of the strain H18 contains a large number of genes related to glucoside hydrolase GH, carbohydrate hydrolase CE and the like, and the content of neutral detergent fibers and lignin in the rice straw can be remarkably reduced by performing fermentation treatment on the rice straw by using the strain H18 through an in-vitro fermentation method. The strain can be used as a bacterial strain for feed application of the rice straws so as to improve the conversion rate and the utilization rate of the rice straws.

The invention provides bacillus licheniformis capable of producing amylase, protease and cellulase and application of the bacillus licheniformis. The bacillus licheniformis is bacillus licheniformis FJAT-27214 and is preserved in the China General Microbiological Culture Collection Center on August 6, 2025, the preservation address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 35536. The bacillus licheniformis disclosed by the invention can be used for simultaneously producing amylase, protease and cellulase at high yield; the bacillus subtilis has antibacterial activity to escherichia coli and has certain tolerance to artificial gastric juice, artificial intestinal juice and cholate, the survival rate in the artificial gastric juice is 58.11%, the survival rate in the artificial intestinal juice is 82.58%, and the survival rate in the cholate is 28.03%. When the strain fermentation liquor is used for pomace micro-storage, the content of crude protein and total sugar of pomace can be remarkably increased, meanwhile, the content of neutral detergent fiber and ammoniacal nitrogen is remarkably reduced, and an effective strain resource is provided for pomace micro-storage fermented feed.

The invention provides a feed additive for increasing the content of n-3 polyunsaturated fatty acids in pork and a compound feed. The feed additive consists of spartina alterniflora powder and a bacterial enzyme additive, the bacterial enzyme additive is prepared from lactobacillus plantarum, aspergillus niger, yarrowia lipolytica, bacillus subtilis, cellulase, beta-mannase and protease; the compound feed comprises a basic ration and the feed additive. Through development and utilization of spartina alterniflora powder and practice, waste is turned into wealth, a new source of the feed additive is expanded, and the feed additive is added into daily ration, so that the digestibility of neutral detergent fibers of the daily ration is improved, the antioxidant capacity of serum is enhanced, and the contents of alpha-linolenic acid, arachidonic acid and eicosapentaenoic acid in pork are increased.

1. Name of the design product: Cellulose Analyzer. 2. Use of the design product: This design product is used for the determination of crude fiber components, dietary fiber, acid / neutral detergent fiber (Van Soest), lignin, cellulose, and hemicellulose. 3. Design key points of the design product: lies in the shape. 4. Picture or photo that best shows the design key points: Perspective view.

The invention relates to the technical field of oat breeding, and discloses a molecular marker related to the content of neutral detergent fibers in oat, a primer combination and application, the molecular marker is an insertion deletion (PAV) marker, the PAV molecular marker is located at a 5D chromosome of an oat reference genome, the physical position is 5D: 424169687, and 161bp deletion is achieved; the national genome database login number of the oat reference genome is GWHGDJH00000000.1, and the national genome database login number of the oat reference genome is According to the molecular marker related to the content of the neutral detergent fibers in the oats, the primer combination and the application, detection can be carried out in the seedling stage of the oats by adopting the molecular marker and the detection method, the breeding period is remarkably shortened, the breeding efficiency is improved, and the breeding cost is reduced; meanwhile, a PCR primer combination designed on the basis of the molecular marker is high in specificity and accuracy, can be used for predicting and screening the neutral detergent fiber content of the feeding oat and is used for genetic breeding of the oat.

The invention provides application of a mixed microbial agent in fermentation of cyperus esculentus cake meal and a method for fermenting the cyperus esculentus cake meal, and belongs to the technical field of feed. The invention provides application of a mixed bacterial agent in fermentation of cyperus esculentus cakes. The mixed bacterial agent comprises bacillus subtilis, lactobacillus plantarum and saccharomyces cerevisiae. The bacillus subtilis, the lactobacillus plantarum and the saccharomyces cerevisiae are combined to be used as the mixed microbial agent for fermenting the cyperus esculentus cake meal, the content of reducing sugar, acetic acid and lactic acid in a fermentation product can be increased, the pH value can be reduced, and the content of crude fat, neutral detergent fibers and acid detergent fibers in the cyperus esculentus cake meal can be reduced. Meanwhile, the dry matter degradation rate, the crude protein degradation rate, the NDF degradation rate, the ADF degradation rate and the like of the cyperus esculentus cake meal fermentation product in the rumen can be increased.

The invention relates to the technical field of animal husbandry, and discloses application of hemicellulose and mannan in regulation of growth performance of sheep. The content of hemicellulose is 10.3% DM or 17% DM, and the addition amount of mannan is 80-160 mg / kg DM. According to the application of the hemicellulose and the mannan in regulating the growth performance of the sheep, the mannan is added into daily ration, so that the digestibility of animals to dry matters, crude proteins, neutral detergent fibers and acid detergent fibers can be increased, and the content of total acid, acetic acid, propionic acid, butyric acid and other volatile fatty acids in rumen can be increased; according to the present invention, with the feed, the richness and the diversity of the flora are increased, the richness of the firmicophyta, the spirochete and the like is improved, the intestinal flora of the sheep is adjusted, the blood sugar is reduced, and the daily ration with different hemicellulose levels has different influences on the growth performance, the rumen microorganisms, the metabolite and the lipid metabolism of the sheep; and a theoretical basis is provided for research and development of the yeast-source functional feed additive.

The invention belongs to the technical field of molecular biology, and particularly discloses an SNP (Single Nucleotide Polymorphism) molecular marker combination related to the content of neutral detergent fibers in oat, a chip and application of the SNP molecular marker combination. The SNP molecular marker combination related to the content of the neutral detergent fibers in the oats comprises 109 SNP molecular markers remarkably related to the content of the high-medium detergent fibers in the oats, the physical positions of the 109 SNP molecular markers are determined by sequence alignment based on an oat reference genome GWHGDJH00000000.1, and site information is specifically shown in a table 1 in the specification. The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker combination related to the content of neutral detergent fibers in oat, a chip and application of the SNP molecular marker combination and the chip, which are used for realizing accurate prediction of the content of neutral detergent fibers in a seedling stage of the oat, remarkably shortening the breeding period of the oat, improving the screening efficiency, reducing the breeding and screening cost and providing technical support for directional breeding of the oat.

The invention discloses a bacillus amyloliquefaciens J1 for high yield of cellulase, which belongs to the field of microbial fermentation engineering and is preserved in China Center for Type Culture Collection, the preservation address is No. 299 in eight road, Wuchang District, Wuhan City, Hubei Province, the preservation date is January 6, 2025, the preservation number is CCTCC NO: M 2025045, and the bacillus amyloliquefaciens J1 is classified and named as bacillus amyloliquefaciens J1. The strain solves the problem that a wild strain lacks high enzyme activity, has the advantages of high growth speed, short metabolic cycle, low requirements on culture conditions and enzyme production conditions, high thermal stability of produced cellulase and the like, and has good probiotic and stress-resistant characteristics. When the method is applied to the distiller's grains, the content of neutral detergent fibers in the distiller's grains can be remarkably reduced, the content of reducing sugar is increased, and the utilization efficiency of the distiller's grains is greatly improved.

The invention discloses a method for rapidly predicting the net utilization rate of phosphorus in cottonseed meal by broiler chickens based on chemical component indexes and application, and belongs to the technical field of feed. The method for rapidly predicting the net utilization rate of the broiler chicken to the phosphorus in the cottonseed meal based on the chemical component indexes comprises the following steps: measuring a crude fiber content CF value, a neutral detergent fiber content NDF value and a free gossypol content FG value in the cottonseed meal to be measured; the calculated values are substituted into a mathematical model: SMA (%) = 60.99-2.697 CF (%) + 1.124 NDF (%)-0.0211 FG (mg / kg); and calculating the phosphorus net utilization rate SMA. The invention further discloses application of the method in preparation of broiler feed. According to the method, the efficient prediction model is constructed by utilizing the chemical component data, the net phosphorus utilization rate of the broiler chicken to the cottonseed meal is accurately estimated, the problems of high professional condition requirements, complicated operation, large workload, relatively long time consumption and the like in a traditional determination method are solved, and the realization of accurate preparation of broiler chicken feed is promoted.

The invention discloses a method for fermenting corn bran through a single strain and application, and belongs to the technical field of fermentation engineering. According to the invention, lactic acid bacteria are used as a fermentation strain, alkaline protease is used as enzymolysis protease, cellulase is added for synergistic fermentation, and the fermented corn bran which can be used as a feed is prepared through a solid-state fermentation process; according to the fermented corn bran prepared by the method disclosed by the invention, the contents of small peptides and organic acids in the corn bran can be well increased, the contents of neutral detergent fibers and acid detergent fibers in the feed are reduced, and the flavor and palatability of the feed are improved.

The invention relates to the technical field of microbial fermentation, in particular to a preparation method and application of a compound microbial agent for fermenting sugarcane yellow leaves. The compound microbial agent is prepared from lactobacillus plantarum R4-30, lactobacillus acidophilus G32, lactobacillus fermentum R6-11, lactococcus lactis G3-1, leuconostoc citreum G7-13, candida boidinii G90-9 and pichia kudriavzevii G90-14, and the compound microbial agent is prepared from lactobacillus plantarum R4-30, lactobacillus acidophilus G32, lactobacillus fermentum R6-11, lactococcus lactis G3-1, leuconostoc citreum G7-13 The preparation method of the composite microbial agent comprises the following steps: (1) activation and rejuvenation of strains; and (2) preparation of the composite microbial agent: uniformly mixing single strain culture solutions according to a mass ratio to obtain the composite microbial agent. After the compound microbial agent provided by the invention is used for fermenting the sugarcane yellow leaves for 8 days, the fermented feed is rich in nutrition and fragrant in smell, the content of crude protein in the fermented feed is increased, the number of moulds is reduced, and the content of butyric acid, neutral detergent fibers and hemicellulose is reduced.

The application discloses a compound microbial agent for improving fiber utilization of broilers and application thereof, and belongs to the technical field of microorganisms. The compound microbial agent is compounded by Lactobacillus casei R7-6 and Lactobacillus rhamnosus R5-8 at a volume ratio of 1:0.5-2. The two known strains are compounded for the first time, and it is found that the compounded strains have a synergistic effect within the range of 1:0.5-2, and the effect is significantly reduced beyond the range. The compound microbial agent has selective fermentation characteristics for different fiber source substrates, and the fermentation effect of the compound microbial agent on purple elephant grass and Guimin elephant grass is significantly better than that on alfalfa and Gegen. After fermentation, the content of crude protein is increased, and the content of neutral detergent fiber is reduced. When the compound microbial agent is used for feeding the purple elephant grass or the Guimin elephant grass, the daily average weight gain of broilers is increased, the feed conversion ratio is reduced, and the abdominal fat rate is reduced. The application provides an efficient microbial solution for high-value utilization of agricultural by-products such as purple elephant grass, Guimin elephant grass and other fibers.

The invention discloses application of jasmine flower residues in preparation of a feed additive for improving the reproductive performance of pigs, and belongs to the technical field of efficient breeding of livestock and poultry. Experiments prove that the jasmine flower residues can significantly increase the total litter size, live litter size, healthy litter size and newborn litter weight of sows, shorten the delivery process and reduce the stillbirth size of the sows; the apparent digestibility of acid detergent fibers, neutral detergent fibers and crude fat is improved; the content of IgA, IgM, IgG and INS in piglet serum is increased, and the content of GH tends to be increased; the activity of T-AOC, SOD and GSH-Px in piglet serum is improved, and the content of oxidative damage indexes MDA, H2O2 and TNF-alpha is remarkably reduced. Therefore, by adding the jasmine flower residues into the feed for the pregnant sows, the reproductive performance and the nutrient apparent digestibility of the sows can be improved, and the immunity, the serum hormone content and the oxidation resistance of offspring piglets can be improved. A new thought is provided for feeding the sows and improving the production performance and the reproductive performance of the sows.

The invention belongs to the technical field of molecular biology, and relates to a molecular marker related to the content of neutral detergent fibers in medicago sativa and application of the molecular marker. The technical problem to be solved by the invention is that the period of a traditional breeding mode is long. The technical scheme of the invention is a molecular marker related to the content of neutral detergent fibers in medicago sativa. The specific information is as follows: chromosome: Chr7; the position is 52012545; sNP (Single Nucleotide Polymorphism) typing: T / G. By adopting the molecular marker and the KASP typing primer disclosed by the invention, genotype screening can be completed in a seedling stage, and selection can be performed without waiting for plant maturity, so that the breeding period is greatly shortened.

The invention provides clostridium butyricum DNCBCR-212 and application thereof, and belongs to the technical field of microorganisms. The clostridium butyricum DNCBCR-212 provided by the invention is preserved in the China General Microbiological Culture Collection Center on March 24, 2023, and the preservation number of the clostridium butyricum DNCBCR-212 is CGMCC (China General Microbiological Culture Collection Center) No.26897. The clostridium butyricum DNCBCR-212 provided by the invention has the advantages that the clostridium butyricum DNCBCR-212 The clostridium butyricum DNCBCR-212 not only can increase the body weight, daily gain and average dry matter feed intake of the calves before weaning and improve the apparent digestibility of neutral detergent fibers, crude proteins and crude fat, but also can improve the oxidation resistance and immune function of the calves before weaning.

The invention discloses a fermented feed mainly prepared from rice bran and a preparation method of the fermented feed. The fermented feed mainly containing rice bran comprises a fermentation raw material, a microbial compound preparation and an enzyme preparation, the fermentation raw material is composed of rice bran, soybean meal and wheat bran, and the microbial compound preparation is composed of a lactic acid bacteria preparation, a bacillus preparation and a saccharomycetes preparation. The lactic acid bacteria preparation, the bacillus preparation and the saccharomycetes preparation are respectively obtained by seed propagation of lactobacillus plantarum, bacillus subtilis and saccharomyces cerevisiae; the enzyme preparation comprises xylanase and pectinase. According to the fermented feed disclosed by the invention, neutral detergent fibers are reduced from 49.68% to 39.73%, the degradation rate is 20.03%, acid detergent fibers are reduced from 34.51% to 22.23%, and the degradation rate is 35.58%; compared with an untreated fermentation raw material with rice bran as a main material, neutral detergent fibers and acid detergent fibers of the fermented feed with rice bran as a main material are degraded by 20.03% and 35.58% respectively, and the content of crude protein and the content of acid soluble protein are increased by 17.48% and 176.27% respectively.

This invention relates to a method for evaluating the spectra of biological material of animal, plant, or mixture thereof, the method comprising the steps of: a) detecting spectrometers in a network formed by at least one spectrometer and an input / output device; b) requesting the respective status of each spectrometer in the network of step a), and displaying the detected spectrometers and their status on the input / output device, wherein the status reflects whether the spectrometer is available for recording spectra; c) receiving a selection from spectrometers available for recording spectra on the input / output device; d) recording the spectrum of the sample material of animal, plant, or mixture thereof on the spectrometer selected in step c); e) predicting at least one parameter from the spectrum of step d) using at least one calibration function and / or calibration plot suitable for predicting the value of the parameter. The values ​​of the parameters, wherein the at least one parameter is selected from the content of at least one amino acid, crude protein content, ammonia content, content of all amino acids containing ammonia, content of all amino acids not containing ammonia, crude fat content, dry matter content, crude ash content, energy content, content of at least one biogenic amine, content of at least one anti-nutritional factor, content of at least one sugar, starch content, crude fiber content, neutral detergent fiber content, acid detergent fiber content, total phosphorus content, phytic acid phosphorus content, reactive lysine content, total lysine content, ratio of reactive lysine content to total lysine content, protein dispersibility index, protein solubility, trypsin inhibitor activity, urease activity, and processing condition index (PCI); and f) displaying the predicted results of step e) on the input / output device.

The invention discloses a compound bacterium enzyme preparation for efficiently degrading shrub lignocellulose and application of the compound bacterium enzyme preparation, and relates to the technical field of agricultural microorganisms. The preparation consists of efficient lignocellulose degrading bacteria obtained by screening and a compound enzyme system (cellulase, xylanase and laccase in a ratio of 5: 3: 2) in a specific ratio, and is prepared by an optimized fermentation process. The viable count of the product is greater than or equal to 1 * 10 < 8 > CFU / g, neutral detergent fibers and acid detergent fibers of shrubs can be respectively reduced by 6.4% and 8.27%, the in-vitro dry matter digestibility is improved by 13%, and the product can be stably preserved for more than 6 months at room temperature. The preparation method comprises fermentation liquor preparation and shrub raw material treatment, is simple and convenient to use, can significantly improve the nutritional value of the shrub feed, reduces the breeding cost, and provides an economic and effective solution for the feed utilization of shrub resources in desertification regions.

The invention discloses application of lactobacillus paracasei in preparation of high-quality broussonetia papyrifera silage, the classification name of the lactobacillus paracasei is Lactobacillus paracasei, the lactobacillus paracasei is preserved in China Center for Type Culture Collection on October 8, 2021, and the preservation number is CCTCC M 20211244. The invention further discloses a preparation method of the high-quality broussonetia papyrifera leaf silage. The silage exogenous additive strain is large in thallus quantity and high in lactic acid production capacity in preparation of high-quality broussonetia papyrifera silage, the pH value can be effectively reduced, generation of ammoniacal nitrogen, acid detergent fibers and neutral detergent fibers can be inhibited, loss of nutrient substances in the silage process can be effectively reduced, and the yield of the broussonetia papyrifera silage is increased. The strain secretes more cellulase / lignin degrading enzymes, can be used for degrading lignocellulose, especially lignocellulose in paper mulberry, so that the fermentation quality is improved, and the strain has a good application prospect.

The invention belongs to the technical field of microorganisms, and discloses a Leuconostoc mesenteroides strain FGR-24 and a silage leavening agent thereof, the Leuconostoc mesenteroides FGR-24 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.33336, and the preservation date is January 8, 2025. The invention further discloses a preparation method of the Leuconostoc mesenteroides strain FGR-24 and a silage leavening agent of the Leuconostoc mesenteroides strain FGR-24. When the silage leavening agent containing the strain is applied to rice silage, neutral detergent fibers and acid detergent fibers of silage are remarkably reduced, the content of lactic acid and acetic acid is greatly increased, the silage is excellent in sense, a high-quality microbial agent is provided for efficient feed utilization of rice straw, the breeding cost is reduced, and the application prospect is wide.

The invention discloses an acid-resistant and high-temperature-resistant cellulose degrading bacterium and application thereof in silage, and belongs to the technical field of functional microorganism screening and application. The strain is bacillus CYC6, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36210. The strain has excellent high temperature resistance and acid resistance, and can still keep good growth and cellulase activity under the high temperature of 40-50 DEG C and the acid environment with the pH value of 3.0-4.0; meanwhile, efficient cellulose degradation capacity is achieved, and the activity of carboxymethyl cellulase reaches 17.209 + / -0.472 U / mL. The invention further provides a method for applying the CYC6 strain to silage, and the inoculum size is 1 * 10 < 8 > CFU per gram of silage raw materials. The CYC6 strain can remarkably reduce the content of neutral detergent fibers and acid detergent fibers in silage, meanwhile, the content of crude protein is increased, the fermentation quality is improved, the pH value is reduced to 3.98, and the content of lactic acid is increased to 12.03%. The technical problem that existing cellulose degrading bacteria are prone to inactivation in the high-temperature acid environment in the ensiling process is solved, and an effective solution is provided for improving the quality of silage.