68 results about "Effective strain" patented technology

Methods of predicting earth stresses in response to pore pressure changes in a hydrocarbon-bearing reservoir within a geomechanical system, include establishing physical boundaries for the geomechanical system and acquiring reservoir characteristics. Geomechanical simulations simulate the effects of changes in reservoir characteristics on stress in rock formations within the physical boundaries to determine the rock formation strength at selected nodes in the reservoir. The strength of the rock formations at the nodes is represented by an effective strain (εeff), which includes a compaction strain (εc) and out-of-plane shear strains (γ1-3, Y2-3) at a nodal point. The methods further include determining an effective strain criteria (εeffcr) from a history of well failures in the physical boundaries. The effective strain (εeffcr) at a selected nodal point is compared with the effective strain criteria (εeffcr) to determine if the effective strain (εeff) exceeds the effective strain criteria (εeffcr).

A method to obtain thin (less than 300 nm) strain-relaxed Si1-xGex buffer layers on Si or silicon-on-insulator (SOI) substrates. These buffer layers have a homogeneous distribution of misfit dislocations that relieve the strain, remarkably smooth surfaces, and a low threading dislocation (TD) density, i.e. less than 106 cm2. The approach begins with the growth of a pseudomorphic or nearly pseudomorphic Si1-xGex layer, i.e., a layer that is free of misfit dislocations, which is then implanted with He or other light elements and subsequently annealed to achieve the substantial strain relaxation. The very effective strain relaxation mechanism operating with this method is dislocation nucleation at He-induced platelets (not bubbles) that lie below the Si / Si1-xGex interface, parallel to the Si(001) surface.

The invention discloses a method for restoring micro-scale polluted reservoir water by using situ bio-contact oxidation. The method comprises the following steps: adopting fiber and sponge combined ball-type packings which are independently developed as carriers to perform screening, placing high effective strain which is obtained through screening in culture solution 'HS enrichment medium' to perform enrichment culture, then inoculating the strain which is through the enrichment culture in 'HSK culture medium' to perform expending cultivation and prepare biological microbial inoculum, placing packings in the microbial inoculum to perform biofilm formation and obtain biological immobilized packings; connecting the prepared biological immobilized packings with a string to fix the packings around the laboratory patented device water-lifting aerator of the invention and adopting water-lifting aeration-situ bio-contact oxidation to perform bioremediation to the micro-scale polluted reservoir water. The method of the invention can be used to effectively reduce the concentrations of COD, N, P and other pollutants in reservoir water and especially effectively lower the total nitrogen content of the micro-scale polluted reservoir water so as to fundamentally resolve the pollution problem of reservoir water, inhibit the growth of algae and control water eutrophication.

An equal channel angular extruding, twisting and deforming mold for large plastic forming process is disclosed. The mold is provided with a male mold (7) and a female mold, an inlet channel (8) and an outlet channel (9) communicated with a corner of the inlet channel (8) are arranged in the female mold; the inlet channel (8) and the outlet channel (9) have the same cross section; the male mold (7) and the inlet channel (8) are in clearance fit, wherein a section of spiral channel (10) composed of spiral slots and having the same cross section with the outlet channel (9) is arranged in the outlet channel (9). In the technical process of the invention, the corner formed by the inlet channel and the outlet channel provides strong shearing function for deformation, the spiral channel provides a large shearing deformation amount while increasing the inner hydrostatic pressure during the deformation of a workpiece, so that the effective strain is accumulated, the deformation evenness, and the room temperature strength and comprehensive mechanical property of deformed metal material are improved, the high-performance metal material is obtained, the processing cost of metal materials is reduced, and the yield is improved.

The invention discloses a bioactive multilayer covering remediation method for contaminated sediments. Bioactive multilayer covering with a synergistic effect of river sand and natural zeolite for fixing high-efficiency nitrifying bacteria and denitrifying bacteria is adopted for reducing the amount of contaminants released towards a water body in the sediments and controlling the eutrophication of the water body. The method comprises the steps of: using the river sand and the biozeolite as covering materials, inoculating highly-effective strain bacteria solution obtained through screening into liquid culture medium, adding enriched nitrifying bacteria and denitrifying bacteria liquid into sterilized eutrophic raw water, pouring turbid raw water into a container containing a certain amount of zeolite after the raw water becomes turbid, forming a biofilm, controlling the concentration of dissolved oxygen (DO) to be 2-3mg / L through an aeration device to accelerate biofilm formation, and after three days of continuous aeration for biofilm formation, using continuous water to wash away bacteria which are adsorbed on the surfaces of fillers. The prepared biozeolite and the clean river sand evenly cover the sediments in a multilayer covering way of laying the biozeolite at a lower layer and the river sand at an upper layer. Therefore, a bioactive multilayer covering technique is used for realizing the in-situ remediation of the contaminated sediments.

The invention discloses pediococcus acidilactici P3-4 which is preserved in a common microorganism center of the Chinese microorganism culture preservation management council. The preservation serial number of the pediococcus acidilactici P3-4 is CGMCC No.10574. A novel method for screening lactic acid bacteria is established based on the combination of the microorganism method and the chemical method, a culture medium is selected through antibiotics for primary screening and secondary screening, and a lactic acid bacterium which can degrade erythromycin and roxithromycin is screened out from soil. A reasonable and effective method for screening a strain for degrading erythromycin and roxithromycin is established, the proper required lactic acid bacterium strain is obtained, primary screening and secondary screening of the lactic acid bacterium for degrading erythromycin and roxithromycin are achieved through separation, purification and screening of lactic acid bacteria, and the safe and effective strain for degrading erythromycin and roxithromycin is obtained through screening. The pediococcus acidilactici P3-4 is used for treatment of pollution of erythromycin and roxithromycin and has the advantages that secondary pollution is avoided, treatment efficiency is high, the application range is wide, and cost is low. More options are provided for treating antibiotic pollution in complicated environments through microorganisms.

The invention discloses a tobacco water retention biological organic fertilizer and a preparation method thereof. The preparation method comprises the following steps: fermenting wastes by using bacillus amyloliquefaciens (IAE), compounding the fermentation product with humic acid, and then secondarily fermenting vinegar residue compost to from a biological organic fertilizer which is suitable for tobaccos and has a water retention effect. In the biological organic fertilizer, the number of effective strains is more than or equal to 0.5*10<8>cfu / g, the content of an effective water retention material is more than or equal to 21g / kg in dry weight, the water content is less than or equal to 30%, and the organic content is more than or equal to 45%. A test shows that after the tobacco is transplanted for 30 days, the water content of 0-10cm of the tobacco field soil in which the biological organic fertilizer is applied is 32.7% higher than that of the soil in which the biological organic fertilizer is not applied and the tobacco plant biomass is increased by 55.6%. The water retention biological organic fertilizer disclosed by the invention can significantly increase the water content of the soil and improve the biomass of tobacco plants at the seedling stage.

The invention relates to a DNA sequence of a fungal strain and a method for identifying variety by means of the sequence. An ITS zone sequence of tremella aurantiabla is shown in SEQ ID NO.1, and an ITS zone sequence of host fungus stereum hirsutum is shown in SEQ ID NO.2. During identification, an ITS zone sequence of a to-be-identified sample is extracted at first and then is respectively compared with the SEQ ID NO.1 sequence and the SEQ ID NO.2 sequence to obtain a result. The method can accurately identify the authenticity of a to-be-identified fungus, and can also carry out accurate identification to artificially cultured yeast-like spore strain of tremella aurantiabla and host fungus stereum hirsutum strain; moreover, the method can accurately distinguish and identify the authenticity and purity of tremella aurantiabla variety or strain, thereby providing a reliable technique for variety identification, protection, strain quality control and effective strain breeding.

The invention provides a method for rapidly screening phosphorus-accumulating bacteria in active sludge, belonging to the technical field of sewage treatment and phosphorus removal. The invention relates to a method for rapidly screening high-efficiency phosphorus-accumulating bacteria in a reinforced biological phosphorus removal system. According to the invention, a reinforced biological phosphorus removal technology is an important phosphorus removal technology in sewage treatment, the phosphorus-accumulating bacteria takes a main removal effect in the technology; and the current strain isolation method has the defects that effect is poor and effective strain number is less. In the invention, a photocopy method coordinated with different culture media is utilized, so that bacterial colony passage is performed in the culture conditions alternating between anaerobism and aerobism; and in the passage process, dyeing with Sudan black and toluidine blue is carried out for observation, white-blue plaque selection is carried out for selecting blue plaque strains to be used as high-efficiency phosphorus removal strains. By using the method, the problems of fussy process, slow screeningand poor pertinence caused by a culture medium and culture conditions are solved, and the high-efficiency phosphorus-accumulating bacteria can be rapidly screened out from the active sludge.

The invention provides a biological seedling raising substrate containing composite microbial flora and a preparation method and application of the biological seedling raising substrate. The biological seedling raising substrate containing the composite microbial flora is prepared by mixing single effective strains of trichoderma guizhouense NJAU 4742, trichoderma asperellum 38 and bacillus megaterium L-69 according to the volume ratio of 1:1-3:1-3. Firstly strains of the trichoderma guizhouense NJAU 4742 and trichoderma asperellum 38 are inoculated into a PDA PDA liquid culture medium for cultivation respectively; strains of the bacillus megaterium L-69 are inoculated into an LB culture medium for cultivation; a bacterial liquid is added into a common seedling raising substrate accordingto the inoculation amount (volume / dry weight) of 5%, the water content is adjusted to 45%, thorough mixing is carried out, and placement is performed for 1 day, so that the biological seedling raisingsubstrate containing a single strain is prepared, then uniform mixing is carried out according to the volume ratio of 1:1:1, and placement is performed for 1 day, so that the biological seedling raising substrate containing the composite microbial flora is prepared. The biological seedling raising substrate containing the composite microbial flora can significantly promote the seedling raising effect of tomatoes and cucumbers.

The invention discloses a method for quickly screening taxol-producing endogenetic fungi. The method quickly screens the taxol-producing endogenetic fungi by adoption of key enzyme genes synthesized by taxol as molecular markers and on the basis of the PCR amplification technology. The method designs primers according to conserved regions of 10-Deacetyl Baccatin III-10-O- acetylase genes and C-13phenylalanine side chain CoA acetylase genes of reported yew, performs amplification screening on fungi which is obtained by separation of yew barks in turn, performs fermentation culture on the fungi which can generate the two genes by means of amplification, extracts the taxol, analyzes the taxol of the fungi by HPLC and MS, and finally determines the taxol-producing endogenetic fungi. The invention provides an effective strain separating and screening method and a batch of effective original strains in order to realize replacement of extraction and conversion of the taxol from yew plant tissue by the microorganism fermentation method.

The invention provides a disc-type strain multiplier for amplifying the surface strain of a mechanical part in real time under a multi-axis load and a design method, and belongs to the technical fieldof fatigue life prediction of the mechanical part. The multi-axis strain condition of the surface of the mechanical part is obtained through a disc type strain multiplier mounting foot mounted on thesurface of the mechanical part, and the disc center rubber body of the disc type strain multiplier is used for mechanically amplifying the average strain of the surface of a mechanical part at the covering position of the disc surface by a certain multiple, so that the fatigue life meter mounted on the multiplier can work within an effective strain amplitude range, and the safe and reliable operation of mechanical equipment is ensured.

The invention discloses a method for inducing endophytic fungi of orchid. The method includes: utilizing a seeding cup to replace a seed bag to perform in-situ symbiosis germination of orchid seed and induce effective endophytic fungi. The method solves the problems about seed clot, low germination rate, proneness to being influenced by weather conditions and the like caused during conventional in-situ symbiosis germination of the orchid. The method is simple to operate, convenient to get materials, low in cost and high in seed emergence rate, seedlings in different growing stages can be selected to perform effective strain separation according to requirements, and a new operable way for protecting endangered orchid and culturing seeding is developed.

The invention provides a biological organic fertilizer. The biological organic fertilizer comprises the following raw materials in parts by mass: 20-25 parts of a nitrogen fertilizer, 5-7 parts of a potash fertilizer, 15-22 parts of a phosphate fertilizer, 1-3 parts of humic acid, 1-6 parts of straws, 2-4 parts of soybean meal, 6-8 parts of rice husk powder, 3-10 parts of clay, 0.5-3 parts of maifan stone, 4-8 parts of ground phosphate rock, 2-3 parts of peat soil, 1-2 parts of zeolite powder, 5-7 parts of earthworm feces, 0.03-0.1 part of strains, and 0.2-0.4 part of a seaweed extraction liquid. The biological organic fertilizer provided by the invention has the effective technical effects that proportioning of the above organic and inorganic components is performed according to a specialratio, the effective strains are added, so that industrial waste can be utilized in an environmentally-friendly manner; and in particular, the seaweed extraction liquid can make the strains stored for a long time, and the strains are uniformly dispersed into each inorganic component through mixing, so that the strains can better transfer beneficial components in the inorganic components, and thebiological organic fertilizer has high stability, high fertilizer efficiency, a long fertilizer effect, and lower costs.

This invention describes discovery and development of a biological system of plant growth promotion and environmental improvement by carbon sequestration and / or nitrogen utilization by application of a microbial agent, e.g., a highly effective strain of T. viride, particularly strains NRRL B-50520 and / or K5. This strain outperforms the best current strains of Trichoderma used for this purpose available commercially. The highly active products also are expected to increase plant productivity and improve quality of fruits, vegetables, flowers or other plant products. The invention also describes and demonstrates that strain combinations including Bacillus amyloliquifaciens AS2 or AS3, as well as disclosed metabolites, promote plant growth. This is true across monocots and dicots, seed treatments and foliar sprays. In the field plant height, shoot weight, root weight, and photosynthesis (Spad meter readings) were all increased in comparison to the untreated control as well as the leading biological seed treatments. The invention also describes metabolite utilization for enhancing plant growth.

The invention discloses a method for separating and screening endophytic fungi of schisandra, and belongs to the technical field of microbes. The method comprises the steps of preparation of schisandra tissues, surface disinfection and sterile detection, separation and culture of the endophytic fungi of the schisandra tissues, preparation of practical fermentation medium, culture of the endophytic fungi, screening of strains and the like. The invention provides the effective strain separating and screening method for extracting anti-tumor active substances from the schisandra plant tissues by substituting a microbial fermentation method.

The invention relates to a preparation method of xylooligosaccharide. The xylooligosaccharide is prepared by hydrolysis fermentation of lignocellulosic biomass materials from one or multiple strains of self-produced cellulase and / or hemicellulase. The high effective strains are adopted, enzyme is hydrolyzed by the aid of the self-secreted cellulase and hemicellulase of the strains, the lignocellulosic materials are degraded by controllable liquid deep hydrolysis fermentation, and reparation of the xylooligosaccharide is achieved; compared with the prior art, the preparation method has the advantages of wide material application range, high yield, simple production process and high efficiency.

The invention discloses a quick start method of a denitrification bioreactor. The quick start method comprises the following steps of: 1) separating out a group of sulfur autotrophic denitrifying bacteria from an experimental denitrification bioreactor; 2) putting the sulfur autotrophic denitrifying bacteria obtained in the step 1) in a culture solution for enlarge cultivation; 3) immobilizing the sulfur autotrophic denitrifying bacteria mixture obtained after enlarge cultivation on a carrier by taking activated carbon or ceramsite as the adsorption carrier and inoculating the bacteria into the denitrification bioreactor, thereby realizing quick start of the denitrification bioreactor. The quick start method of the denitrification bioreactor provided by the invention has the following advantages that the mixture of a plurality of sulfur autotrophic denitrifying effective strains is obtained through culture on the basis of experimental study, the optimal condition for growth of the microbial population is found out, and an appropriate carrier is utilized so that the microbial population grows quickly under appropriate growth conditions, and therefore, lots of functional microorganisms can be obtained in short time for inoculation of the sulfur autotrophic denitrification bioreactor; as a result, the time of starting the reactor is greatly shortened.

The invention discloses a method for rapidly screening produced polyunsaturated fatty acid. In the method, by taking a key enzyme gene as a molecular marker, microbes producing the polyunsaturated fatty acid can be rapidly and directionally screened on the basis of a PCR amplification technology. The method comprises the following steps of: designing a primer according to conserved regions of reported delta 6 fatty acid desaturase (D6) and delta 5 fatty acid desaturase gene (D5), amplifying and screening microbes obtained by separating soil or a water body in sequence to produce GLA by amplifying the gene of D6 and to produce AA and EPA by amplifying the genes of D6 and D5 simultaneously; then fermenting and culturing the microbes capable of producing the gene of D6 and simultaneously producing the genes of D6 and D5 by amplifying and extracting fatty acid; analyzing the fatty acid synthesized by the microbes by using GC and MS; and finally determining the microbe producing the polyunsaturated fatty acid. The invention provides an effective strain or algae seed separating and screening method and a batch of effective starting strains or algae seeds for producing the polyunsaturated fatty acid by using micro to ferment.

The invention relates to a measurement and calibration integrated marine fiber bragg grating strain sensor system. The system is composed of a fiber bragg grating strain sensor and a sensor calibration device. Two ends of a fiber bragg grating are fixedly connected with a sensor fixed end and a sensor movable end. The sensor fixed end is fixedly connected with a positioning plate through utilization of a fixed end welding base. The sensor movable end is connected with the positioning plate through utilization of a movable end welding base. The movable end welding base can move front and back along the positioning plate, so a distance between two fixed points of the fiber bragg grating is an effective strain length L. A high-precision differential screw is fixedly connected with the positioning plate through utilization of a screw mounting base. The screw mounting base and the movable end welding base are connected through utilization of a return spring. Through matching of the high-precision differential screw and the return spring, the sensor movable end can be controlled to slide front and back and sliding displacement deltaL of the sensor movable end can be precisely controlled,so a standard dependent variable epsilon=deltaL / L is applied to the fiber bragg grating strain sensor, and the fiber bragg grating strain sensor is mounted or calibrated.

The invention discloses a high-impact piezoelectric accelerometer. The high-impact piezoelectric accelerometer comprises a hollow installing seat, a core is arranged in the hollow installing seat through a compressing ring in a press fit mode and is connected with a cable through a plug, one shock insulation cushion and one insulating cushion are arranged between one end of the core and the compressing ring, and another shock insulation cushion and another insulating cushion are arranged between the other end of the core and the installing seat. Because the shock insulation cushions large in damping are installed at the two ends of the core of the piezoelectric accelerometer, high-frequency large-amplitude acceleration generated by strong-impact waves on a structure can be filtered out, and transverse acceleration transmitted to an acceleration sensor can be reduced so as to achieve the purpose of reducing zero drift. Due to the adoption of a base strain isolation type structure, strain of sensitive elements can be effectively reduced; because the insulating cushions are arranged at the two ends of the core and serve as isolators, the accelerometer can be isolated from an installing face mechanically so that effective strain transmitted to the accelerometer can be reduced and the purpose of reducing zero drift can be achieved.

A production method for an Fe—Ni based heat-resistant superalloy inhibits abnormal grain growth and yields a fine crystal grain structure having an ASTM crystal grain size number of 9 or greater. The production method comprises at least a hot working step in which a material having a prescribed composition is subjected to hot working, wherein the hot working step includes at least a step in which the above material of 930 to 1010° C. is subjected to hot working so that the relation of (effective strain)≧0.139×(effective strain rate( / sec))−0.30 is satisfied in the entirety of the above material.

The invention discloses a special-shaped blank continuous casting secondary-cooling roll gap adjustment method which includes the steps: (1), dividing four heat-transfer areas between two pairs of pinch rolls in a continuous casting drawing direction of a special-shaped blank, respectively calculating length and heat flux of each heat-transfer area and calculating water density distribution along the circumference of the casting blank section within a water spraying area and area value of the water spraying area; (2), constructing a two-dimensional heat-transfer model and calculating to obtain distribution of temperature of a casting blank at different solidification stages; (3), according to parameters of mechanical behavior under high temperature and phase transformation behavior of steel grades, building a special-shaped blank continuous casting strain model under high-temperature stress and calculating effective strains of the casting blank at different solidification stages; (4), according to the effective strains, calculating instantaneous strain rate of the casting blank so as to calculate and make a secondary-cooling roll gap shrinkage curve of feature positions of the continuous casting blank of the special-shaped blank.The roll gap shrinkage curve provides a theoretical direction to arrangement and adjustment of roll lines during actual production of special-shaped blank continuous casting.The method has the advantages of improving adjustment precision of the secondary-cooling roll gaps and effectively controlling and improving continuous casting quality of the special-shaped blank.

The invention discloses a special-shaped blank continuous casting secondary-cooling roll gap adjustment method which includes the steps: (1), dividing four heat-transfer areas between two pairs of pinch rolls in a continuous casting drawing direction of a special-shaped blank, respectively calculating length and heat flux of each heat-transfer area and calculating water density distribution along the circumference of the casting blank section within a water spraying area and area value of the water spraying area; (2), constructing a two-dimensional heat-transfer model and calculating to obtain distribution of temperature of a casting blank at different solidification stages; (3), according to parameters of mechanical behavior under high temperature and phase transformation behavior of steel grades, building a special-shaped blank continuous casting strain model under high-temperature stress and calculating effective strains of the casting blank at different solidification stages; (4), according to the effective strains, calculating instantaneous strain rate of the casting blank so as to calculate and make a secondary-cooling roll gap shrinkage curve of feature positions of the continuous casting blank of the special-shaped blank.The roll gap shrinkage curve provides a theoretical direction to arrangement and adjustment of roll lines during actual production of special-shaped blank continuous casting.The method has the advantages of improving adjustment precision of the secondary-cooling roll gaps and effectively controlling and improving continuous casting quality of the special-shaped blank.

The invention relates to a molecular identification method of a ribosome large-subunit gene D1 / D2 region sequence of a golden tremella and a host fungi crude Stereum hirsutum. The ribosome large-subunit gene sequence of the golden tremella is shown in SEQ ID NO.1, and the ribosome large-subunit gene D1 / D2 region sequence of the host fungi crude Stereum hirsutum is shown in SEQ ID NO.2. The molecular identification method has the steps as follows: the ribosome large-subunit gene D1 / D2 region sequence of the strains to be identified is obtained after extraction, and is judged through being compared with the sequence in the SEQ ID NO.1 and the SEQ ID NO.2, so as to judge whether the strains to be identified is the golden tremella strains or host fungi crude Stereum hirsutum strains. By utilizing the molecular identification method, the golden tremella strains and the host fungi crude Stereum hirsutum strains which are cultured artificially can be identified accurately; the reliable technology is provided for strains identification, breed conservation, strain quality control, effective strain propagation, and artificial cultivation of the golden tremella.

The invention discloses a fermented fresh flower and various strain compound microbial fermented sweet white spirit and a preparation method thereof. Conventional distiller's yeast is complicated in infectious microbe, relatively heavy in pollution and difficult to control fermentation, so that fermentation always fails to lead to a lot of waste. The raw materials used by the sweet white spirit disclosed by the invention comprise the following components in parts by weight: 100 parts of sticky rice and 0.4-0.7 part of compound fermenting microorganisms, wherein the proportion of ferment edible fresh flowers to a sticky rice fermented semiproduct is 1: 9. The sticky rice and the fresh flowers which are treated are separately fermented, mixed, bottled and sterilized and the like to prepare the fermented fresh flower and various strain compound microbial fermented sweet white spirit. The preparation method comprises the following steps: treating raw materials; steaming the raw materials in a cage; performing fermentation; and mixing the raw materials. By performing fermentation, separation and purification for many times by using conventional distiller's yeast, effective strains in the distiller's yeast are bred and cultured. Single strains are independently cultured, and are proportioned in a certain proportion for fermenting the sweet white spirit, so that fragrance and health-care function of the sweet white spirit and special fragrance and health-care function of fermented fresh flowers are organically combined to obtain novel health-care sweet white spirit.

The invention discloses a design method for reducing the discharging quantity of spider cold forging blank. The method mainly comprises a step (1) of calculating difference value delta H between the theoretic discharging quantity H1 and the actual discharge rate H2 according to a detail drawing; a step (2) of building a three-dimensional solid model by utilizing SOLIDWORKS; a step (3) of performing finite element modeling on the spider forming process through DEFORM-3D finite element modeling software to enable the delta H to reduce gradually until the H2 closes to the H1; and a step (4) of performing equivalent stress, effective strain and load displacement curve analysis on an optimized finite element model with the blank quantity of HX and changing technological parameters of a die corner R and the draft angle V to enable molded parts to meet the production requirements. According to the design method, the flow of metal and reasonable forming technology parameters in the spider cold forging modeling can be analyzed accurately, the discharging quantity of the blank is reduced, the microhardness and the strength of the parts after undergoing subzero treatment are improved remarkably, and the parts have good plasticity.

A method which uses a molecular marker technology for identifying Pleurotus citrinopileatus comprises the cultivation and collection of mycelium, the extraction of genome DNA, the establishment of the detection of a SCAR-PCR molecular marker, and the detection of a SCAR-PCR product. The method has the advantages of high sensitivity, less usage of needed DNA, and short detection time, high accuracy, easy judgment, good reproducibility, and the like, compared with conventional morphology detection, antagonistic test, and fruiting test. The method provides an effective strain identification method for the use of Pleurotus citrinopileatus germplasm resources and the registration work of new varieties.