51 results about "Fat grafting" patented technology

A closed system for harvesting fat through liposuction, concentrating the aspirate so obtained, and then re-injecting the concentrated fat into a patient comprises as its main components a low pressure cannula having between about 7 to 12 side holes of about 1-2 mm by 2.0 to 4.0 mm, a spring loaded syringe holder with a helicoidal spring to apply a substantially constant pressure over the full excursion of the plunger, and a preferably flexible collection bag which is also preferably graduated, cylindrical over most of its body and funnel shaped at its bottom, all of which are connected through flexible tubings to a multi-port valve. The multi-port valve has two flutter/duck bill valves which restrict the fluid flow to a one way direction which effectively allows the syringe to be used to pump fat out of a patient and into a collection bag in a continuous manner. After the bags are centrifuged to concentrate the fat, the excess fluids are separated and the valve is re-connected to permit the syringe pump to reverse fluid flow to graft the concentrated fat back into the patient.

Methods for lipomodeling by peripherally administering a modulator of a Y receptor are provided. Methods may comprise reduction of a fat depot by administering a Y receptor antagonist proximally and/or directly to the site of the fat depot. Other methods comprise increasing or stabilizing a fat depot or fat graft by administering a Y receptor agonist proximally and/or directly to the site of the fat depot or fat graft. Also provided are methods for stimulating wound healing by administering a Y receptor agonist proximally to a wound site.

A portable apparatus for collection and processing of human biological material, such as containing adipose extracted during a lipoplasty procedure, is useful for multi-step processing to prepare a concentrated product (e.g., stromal vascular fraction) or a fat graft composition. The apparatus has a container with a containment volume with a tissue retention volume and a filtrate volume separated by a filter and with a tapered portion to a collection volume for collecting concentrate product. Inlet and suction ports provide access to the tissue retention volume and filtrate volume, respectively, and an extraction port provides versatile access for removal of target processed concentrate material or fat graft material, which access may be via a lumen through a rotatable mixer shaft. Access ports may be configured for access only from above the container. A method of processing adipose tissue to concentrate leuko stromal vascular cells includes multi-step processing using a portable container.

A portable apparatus useful for collection and processing of human biological material containing adipose, such as extracted during a lipoplasty procedure to prepare a concentrated product (e.g., stromal vascular fraction) or a fat graft composition. The apparatus has a container with a containment volume with a tissue retention volume and a filtrate volume separated by a filter and with a tapered portion to a collection volume for collecting concentrate product. Inlet and suction ports provide access to the tissue retention volume and filtrate volume, respectively, and an extraction port provides versatile access for removal of target processed concentrate material or fat graft material, which access may be via a lumen through a rotatable mixer shaft. Access ports may be configured for access only from above the container. The apparatus may include a tissue collector disposed in the disuse retention volume to engage and collect collagen or other stringy tissue. A method of processing adipose tissue to concentrate leuko stromal vascular cells includes multi-step processing using a portable container.

The invention discloses a method for efficiently preparing a fat source biomaterial by using ultrasonic wave. The method comprises the steps of centrifugation, ultrasonic wave breaking (including contact type and non-contact type ultrasonic wave), centrifugal collection and the like. According to the method, the ultrasonic wave is utilized to break most mature fat cells in fat tissues, and the flocculation centrifugal sedimentation technology is applied to collect oil droplets generated by breaking the mature fat cells to achieve the purpose of enriching SVFs; high-speed centrifuging is utilized to remove cellular components to obtain ECM. After ultrasonic treatment, the cell activity is greatly improved, and the apoptosis rate is greatly reduced, so that the effectiveness of the transplantation is greatly improved. At the same time, an ECM biomacromolecule material can be obtained, and the low ECM content and the cytokine loss caused by the cumbersome operation are avoided. The biomaterial obtained by the method can be used for the autologous transplantation biological therapy, reduces the complications of fat transplantation and improves the survival rate of transplanted fat.

The invention discloses a preparation method of adipose gel enriched in an adipose-derived stem cell and an extracellular matrix. A mature adipose cell and the extracellular matrix in an adipose transplanting material are crushed through a physical cutting method; the filtration and the emulsification are carried out by using an adipose emulsifier, so as to remove an uncrushed component in adipose and obtain chylous adipose; by adopting a flocculation precipitation technique, grease in the chylous adipose is collected and removed by using polyethylene glycol, so as to obtain the adipose gel rich in the adipose-derived stem cell and the extracellular matrix. According to the adipose gel obtained by the method, as the mature adipose cell and the grease are removed, the volume of original adipose is concentrated for approximately 10 times; the content of the adipose-derived stem cell and the extracellular matrix is far higher than that of common adipose; a favorable material is provided for the transplantation and filling of the adipose.

The invention discloses equipment and method for increasing the transplanting success rate of autogenous fat, and particularly relates to the field of fat transplanting. The equipment comprises a device barrel. A heat insulating layer is arranged on the inner wall of the device barrel. The device barrel comprises a standing cover barrel, a rubber column is embedded into the top end of the standingcover barrel, a first semiconductor heating piece is arranged in the standing cover barrel, and a first liquidometer and a second liquidometer are arranged in an inner cavity of the standing cover barrel. A standing barrel is arranged at the bottom of the standing cover barrel, an isolating layer is arranged in an inner cavity of the standing barrel, a leak pipe penetrates through the isolating layer, and an electromagnetic valve is arranged in the leak pipe. A sponge filtering barrel is arranged at the bottom of the standing barrel. The effect of separating fat particles from liquid, greaseand other impurities is achieved, the fat particles with fewer impurities and high purity are obtained, cells are not prone to breakage in the purification process, the integrity of the cells is guaranteed, thus, the survival rate of fat transplanting is increased, and the fat absorption rate is reduced.

A method of conditioning a soft tissue region or site of a patient prior to, during or after a fat grafting procedure, or any combination of these, has the steps of: activating an ultrasonic or acoustic shock wave generator or source to emit ultrasonic or acoustic shock waves; subjecting the soft tissue region or site to ultrasonic or acoustic shock waves stimulating the said soft tissue; wherein the emitted waves are focused or unfocused low energy acoustic shock waves or ultrasonic waves and wherein the soft tissue region or site underlies the patient's skin. The soft tissue region or site is one of a buttock, a breast or a scalp. The method of conditioning a soft tissue region or site further has the step of: treating the soft tissue region or site after conditioning and implanting or injecting viable fat cells in a fat grafting procedure.

Methods and devices for treating lipoaspirate for use in fat grafting procedures are provided and generally include a canister for containing lipoaspirate, a separation mechanism structured to separate both oils and other materials from cellular components of lipoaspirate contained in the canister. The separation mechanism includes filters having different filtering capacities, for example, different pore sizes.

The invention discloses a fat particle tissue cryopreservation liquid, a preparation method and a freeze-saving method thereof. The fat particle tissue freeze-saving liquid comprises trehalose, hydroxyethyl starch, L-ascorbic acid, albumin, human insulin, a basic fibroblast growth factor and a basic culture medium. The fat particle tissue freeze-saving liquid does not contain dimethyl sulfoxide and fetal bovine serum, has no toxicity to cells, does not denature proteins, is safe to use, can enable frozen fat particle tissue to have higher vitality, improves the survival rate of fat tissue after recovery, can enable fat grafts to maintain higher biological activity and tissue structure, and can be widely applied in clinic.

The invention discloses a liposuction device for autologous fat transplantation of the human body and a usage method thereof and belongs to the field of medical surgical operation devices. A fat quantification device and a fat storage device are arranged in an incubator, and a temperature sensor I and a temperature sensor II are arranged in a liposuction end head and the incubator respectively. A pressure pump switch for starting a pressure pump or not through controlling, a pressure display screen and a pressure regulating knob are all arranged on the pressure pump. A thin water body impact tube is arranged on a delivery pipeline between the fat quantification device and the fat storage device. A primary filter layer and a secondary filter layer are sequentially arranged in the fat storage device from top to bottom. A more suitable storage environment can be provided for fat cells, and keeping of the activity of the fat cells is promoted. Misoperations produced by excessive and easily mistaken switching and the situation that normal operation cannot be performed in time can be prevented. The keeping of the survival rate of the fat cells is promoted. The accuracy of fat quantification can be ensured, and collection and reutilization of the fat cells and residual materials are promoted.

The invention provides a multifunctional autologous fat transplantation device maintaining fat cell activity for a short period. The device comprises a needle guard, a syringe needle, and an injector body. The injector body comprises a needle cylinder and a plunger. The front end of the needle cylinder is provided with a needle tubing connected with the syringe needle. The needle cylinder is provided with scales. The needle cylinder is internally provided with a filter screen fixed on the inner wall of the needle cylinder. The needle cylinder is provided with a cell culture fluid storage tank. The cell culture fluid storage tank is internally provided with a fat cell culture fluid. The cell culture fluid storage tank and the needle cylinder are connected through a first solenoid valve. The invention provides the multifunctional device which integrates fat cell extraction, analysis, and injection, and the device provides stable environment which maintains fat cell activity for a short period. One-time extraction of fat cells and low-intensity and high-frequency injection can be performed, and steps of repeated extraction are reduced, and effect of low-intensity and high-frequency injection is improved.

The embodiment of the invention provides a cell-assisted autologous fat transplant for injection and a preparation method thereof, and relates to the field of medical plastic and aesthetic surgery. The cell-assisted autologous fat transplant for the injection can increase the survival rate of autologous fat transplantation, and obviously improve the effect after fat transplantation. The cell-assisted autologous fat transplant for the injection comprises autologous fat particles, mesenchymal stem cell aggregations and autologous plasma PRP rich in blood platelets.

The invention belongs to the technical field of regenerative medicine and discloses a method for preparing an adipose-derived ECM (extracellular matrix) through built-in ultrasonic waves, which comprises steps of centrifugation, ultrasonic crushing, centrifugal collection and the like. According to the preparation method, most mature adipose cells in adipose tissue are crushed through ultrasonic waves, manure adipose cells and SVF cells are removed, the ECM is prepared, and too low ECM content and cell factor loss caused by complex operation are avoided effectively. According to the method, the ECM rich in cell factors is prepared without addition of exogenous chemical substances, the prepared ECM contains no exogenous substances, and adipose autotransplantation and effectiveness of the ECM as a biological filling material can be improved. The adipose-derived ECM material prepared with the method can be applied to autotransplantation stem cell therapy, reduces complications caused by adipose transplantation, increases the survival rate of transplanted adipose and has broad application prospects.

The present invention provides for methods of treating diabetes by providing to a subject brown adipose tissue. The tissue may be transplanted from a culture of the subject's own tissue (autograft) or from a donor. These methods can achieve normoglycemia and suppress hypergluconemia, and alleviate conditions associated with such, even in the absence of or at extremely low levels of adjunct insulin therapy, and without any appreciable increase in insulinogenesis.