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Cell-assisted autologous fat transplant for injection and preparation method thereof

A technique for grafts and injections, applied in the field of cell-assisted autologous fat grafts for injections and its preparation, can solve problems such as the ineffectiveness of CAL transplantation, the destruction of natural adipose tissue structure, and difficulty in participating in blood supply reconstruction, etc., to achieve Ease of vascularization, no immune rejection, easy access to results

Inactive Publication Date: 2018-10-26
广东芙金干细胞再生医学有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a large sample comparison found that the transplantation effect of CAL was not significantly better than the traditional autologous fat grafting method
Reasons for analysis: During the construction of fat grafts, the natural fat tissue structure is damaged and lacks support, so that the added cells are wrapped in fat particles, making it difficult to participate in blood supply reconstruction, which eventually leads to graft center necrosis and graft failure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] An embodiment of the present invention provides a cell-assisted autologous fat graft for injection, which includes autologous fat particles, mesenchymal stem cell aggregates and autologous platelet-rich plasma PRP.

[0036] In an optional embodiment, the mesenchymal stem cell aggregate has a certain mechanical strength.

[0037] Further, the mesenchymal stem cell aggregates at least include collagen.

[0038] In another optional embodiment, the autologous platelet-rich plasma PRP includes at least transforming growth factor β-1 (TGFβ-1), platelet-derived growth factors (PDGFs) and insulin-like growth factors (IGFs) .

[0039] Further, the autologous platelet-rich plasma PRP has the function of fibrin scaffolding and sustained release.

[0040] The method is to add cell aggregates and platelet-rich plasma to autologous fat particles, and the cell aggregates play an internal support role in the fat graft and provide space for the nutrient exchange of fat tissue. Variou...

Embodiment 2

[0042] The embodiment of the present invention also provides a preparation method of cell-assisted autologous fat graft for injection, the preparation method of cell-assisted autologous fat graft for injection at least includes the following steps:

[0043] Step 1: Purification of autologous fat particles

[0044] Process the adipose tissue obtained by liposuction to obtain solid fat particles; then remove the connective tissue from the obtained fat particles, cut up the large pieces of tissue with tissue scissors, and use a 1 ml syringe to remove the needle and extract it normally;

[0045] Step 2: Preparation of mesenchymal stem cell aggregates

[0046] The mesenchymal stem cells were seeded in disposable culture dishes at 37°C, 5% CO 2The cell membrane was cultured under certain conditions; after 7-10 days of culture in DMEM high-glucose medium, the cell membrane was formed; the cell membrane was lifted from the edge, and divided into mesenchymal stem cell aggregates with ...

Embodiment 3

[0060] The autologous fat graft of the present invention comprises autologous fat particles, aggregates of mesenchymal stem cells and autologous platelet-rich plasma. The build method is as follows:

[0061] Step 1: Purification of autologous fat particles

[0062] Centrifuge the adipose tissue obtained by liposuction at 1000-2000 r / min at 21°C for 2-10 minutes to remove the liquid part to obtain solid fat particles; then remove the connective tissue from the obtained fat particles and cut the large pieces of tissue into pieces with tissue scissors , it is advisable to use a 1 ml syringe to remove the needle and extract normally; the purpose of centrifuging the adipose tissue is to remove the swelling fluid injected during anesthesia, so that the purity of the adipose tissue is higher; the removal of connective tissue is to make the subsequent transplantation materials mix uniformly and easily injection.

[0063] Step 2: Preparation of mesenchymal stem cell aggregates

[00...

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Abstract

The embodiment of the invention provides a cell-assisted autologous fat transplant for injection and a preparation method thereof, and relates to the field of medical plastic and aesthetic surgery. The cell-assisted autologous fat transplant for the injection can increase the survival rate of autologous fat transplantation, and obviously improve the effect after fat transplantation. The cell-assisted autologous fat transplant for the injection comprises autologous fat particles, mesenchymal stem cell aggregations and autologous plasma PRP rich in blood platelets.

Description

technical field [0001] The invention relates to the field of medical plastic surgery and cosmetology, in particular to a cell-assisted autologous fat graft for injection and a preparation method thereof. Background technique [0002] Soft tissue grafts use the mass effect to support the tissue structure, repair soft tissue loss, remove depressions, and can also increase the volume of the breast or buttocks to achieve the purpose of reshaping the shape. A good soft tissue graft needs to have the following characteristics: (1) good tissue compatibility without causing inflammation; (2) able to integrate with the filling site; (3) durable and reliable filling effect. [0003] Autologous fat was first used as a soft tissue graft in 1893, when Neuber used the patient's autologous fat to fill sunken facial defects. Compared with collagen, hyaluronic acid, PMMA, polyacrylamide and other gel soft tissue fillers, autologous fat has obvious advantages: it can be obtained by liposucti...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/38A61L27/50
CPCA61L27/3604A61L27/3616A61L27/3834A61L27/3895A61L27/50A61L2400/06
Inventor 朱君邓志宏赵宇
Owner 广东芙金干细胞再生医学有限公司
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