70results about How to "Not transgender" patented technology

The invention discloses an electronic cigarette which comprises a cigarette-like shell with a hollow cavity, an electrical source, a cigarette pellet and cigarette rod fittings. The electrical source, a pneumatic switch, a magnetostrictive vibrator and an electromagnetic induction heater are arranged in sequence inside the shell from the front end to the back end; wherein, a high frequency generator is respectively connected with the magnetostrictive vibrator, the electromagnetic induction heater and the pneumatic switch through leads. A light-emitting diode is arranged at the front end of the shell and is respectively connected with the pneumatic switch and the electric source through leads. The back end of the shell is connected with the cigarette pellet in which a tobacco solution chamber is arranged.

The invention discloses a method for extracting rice protein by using rice, which comprises the following steps: soaking and grinding, rice milk adjustment, primary liquefying, centrifugal separation, homogenizing, washing, centrifuging, enzymolysis, enzyme inactivation, screen separation, washing, centrifuging, drying, crushing and packing so as to obtain the rice protein. The method overcomes the defects that the high-concentration alkali liquor has damage effect on rice protein amino acid in an alkali extraction process, the quality of the extracted rice protein is poor, and the purity and sanitary index of the extracted protein cannot reach the edible standard; and in other methods for extracting the rice protein by using enzyme preparations, the rice milk is liquefied at a temperature of over 95 DEG C and then is dried at the high temperature, so the rice protein is denatured, the color is darkened, and the function of the rice protein is damaged. The extracting method of the invention keeps good physical functionality of the rice protein, implements industrialization of the extraction and production of the rice protein, and meanwhile improves the production conversion rate and quality of rice starch sugar.

The invention provides a formula of a low glycemic index (GI) food and a preparation method thereof. The preparation method of the low GI food formula comprises the following steps: uniformly mixing the following components in percentage by weight: 5-20% of wheat gluten powder, 10-50% of oat powder, 5-50% of buckwheat powder, 10-50% of chickpea flour, 10-50% of hyacinth bean powder and 0-15% of soybean powder; and performing dough kneading, and forming a network structure by virtue of gluten protein in the wheat gluten powder in low-temperature extrusion molding conditions to produce the low GI food. By adopting the characteristics that the gluten network structure is prepared from the gluten protein of the wheat gluten powder in the low-temperature extrusion process, the low GI food is prepared from the wheat gluten powder, various coarse cereal and mixed beans with low GI, and auxiliary materials as main raw materials and performing the extrusion molding at low temperature; and the low GI food prepared by using the preparation method provided by the invention is good in color and taste and smooth in surface, does not have cracks, and is good in texture and density.

The invention discloses a method for extracting total flavonoids in grape seeds in a double-aqueous phase system, belonging to the field of separation and extraction methods of natural product active ingredients. The method comprises the following steps of: firstly preparing grape seed rough extract from grape seeds with ethanol; then adding absolute ethyl alcohol, ammonia sulfate, inorganic salt and water in the grape seed rough extract to form the double-aqueous phase system, mixing uniformly, standing for 5-30 minutes at room temperature for delamination to obtain an upper phase and a lower phase, concentrating the upper phase through pressure reduction and drying so as to obtain total flavonoids of grape seed. In the double-aqueous phase system, the mass percents of grape seed rough extract, absolute ethyl alcohol, ammonia sulfate and inorganic salt are respectively 5% to 30%, 10% to 30%, 16% to 30%, and 0 to 5%, the balance is supplemented to be 100% with water, and the pH of the double-aqueous phase system is 3.0 to 11.0. Flavonoids are extracted by utilizing double aqueous phases in the method; the method is simple, the operation is convenient, the production cost is low and the treating capacity is large, small test data can be directly amplified, and the yield of flavonoids in the grape seeds reaches up to 98.2%; and the method can be operated continuously, and is suitable for industrialized production of flavonoids in the grape seeds.

The invention relates to a drying process of a specific plant. The process comprises the following steps of: 1. pretreatment: sorting prepared fresh pseudo-ginsengs; after separating and removing pruning openings, fibrous roots and flocking roots attached to the fresh pseudo-ginseng roots, obtaining main pseudo-ginseng roots; cleaning and removing surface attachments and leaching surface water; 2. pre-freezing: directly placing the whole main pseudo-ginseng roots into a pre-freezing chamber at minus 15-minus 20 DEG C and pre-freezing for 6-7 hours; 3. sublimation drying: spreading the pre-frozen main pseudo-ginseng roots on a table disk made of heat conducting materials, controlling the thickness of a paving material to be below 5cm and shifting to a vacuum drying chamber; after sealing, vacuumizing, maintaining internal vacuum pressure at 30-70Pa, setting the temperature of the table disk to be 40 DEG C, continuously supplying heat to the table disk and carrying out sublimation drying in vacuum for 10-15 hours till the table disk is heated to 40 DEG C; 4. analysis: maintaining the temperature of the table disk to be 40 DEG C and continuously supplying heat to the table disk and vacuumizing for 10-12 hours till the material is heated to 40 DEG C and material water is controlled to be below 3 percent; and 5. discharging and packaging. The invention provides a method for preparing active pseudo-ginseng.

The present invention relates to a preparation technology for set yogurt with high stability, and belongs to the field of dairy processing. The preparation technology for set yogurt with high stability comprises the following steps: adding 0.01-0.05 wt.% of casein protein powder into standardized and detected raw milk; homogenizing; pasteurizing; inoculating: adding 0.01-0.05 wt.% of beta-lactoglobulin into the pasteurized milk, inoculating lactic acid bacteria, and uniformly mixing the inoculated milk; and conducting fermentation. According to the preparation technology for set yogurt, the prepared set yogurt has good stability, does not contain any stabilizers, has thick and delicate mouth feel and pleasant aroma.

The invention relates to water-based ABS (acrylonitrile butadiene styrene) glue for transfer printing and a preparation method thereof; the water-based ABS (acrylonitrile butadiene styrene) glue comprises the following components by weight: 20-60 parts of self emulsifying polyurethane water dispersion, 0-40 parts of acrylic emulsion, 40-80 parts of deionized water, 0-10 parts of a wetting dispersing agent, 0-5 parts of a leveling agent and 0-3 parts of a defoaming agent; the raw materials are prepared according to the formula, then are stirred and mixed evenly then under normal temperature and pressure for coating a transfer printing substrate. Compared with the prior art, the production process of the water-based ABS (acrylonitrile butadiene styrene) glue completely does not use an organic solvent, and has advantages of simple operation and low cost and the like.

The invention discloses a coaxial electrospinning process of a fibroin-polycaprolactone bi-component ultrafine fiber. By optimizing the inner layer polycaprolactone solution concentration and the solvent component proportion, an inner layer polycaprolactone spinning solution and an outer layer fibroin spinning solution almost volatilize synchronously in a synchronous stretching process, and the incidence rate of coating rate decline caused by outer layer breakage due to earlier stretch stop than the inner layer can be reduced. The process provided by the invention employs the laboratory optimized concentration parameters corresponding to the inner layer solution and the outer layer solution, makes preliminary adjustment for almost synchronous volatilization of the inner layer and the outer layer, then adjusts the dosage of the dichloromethane solvent in the mixed solvent of the inner layer solution to regulate the solvent volatilization speed of the inner layer solution, and makes second fine adjustment for almost synchronous volatilization of the inner layer and the outer layer. The process provided by the invention has the advantages of simple operation method and easy realization, greatly improves the coating rate of the fibroin-polycaprolactone bi-component ultrafine fiber, and increases the stability of the fiber during application in bionic engineering.

The invention discloses a silk weight gaining method adopting a sol-gel method to fix sericin, which includes the following steps of: 1) impurity removal and clearing treatment of silk; 2) preparation of finishing liquid of sol; and 3) fixation and finishing of sericin: silk made by the step 2 is fixed and finished by finishing liquid of sol according to a secondary leaching and rolling method so that liquid carrying rate of silk can reach sixty percent to one hundred percent; then finished silk is aged for zero to twenty four hours at normal temperature; finally the silk is baked for ten to sixty minutes at a temperature between 120 and 180DEG C. The silk weight gaining method has the advantages of safety, environmental protection and effective fixation of silk.

The invention provides a DNA (desoxyribonucleic acid) eluent. The eluent comprises an acid-base buffer solution and an ionic liquid, wherein the ionic liquid is a compound containing imidazolyl cations; the pH value of the eluent is 5 to 13. The eluent provided by the invention comprises the compound containing imidazolyl cations, wherein the compound interacts with negatively charged DNA; the DNA is eluted off from the surface of a solid phase adsorbing material; based on the pH value of the eluent, the electrostatic attraction between the solid phase adsorbing material and DNA fragments is lowered and the elution capability of the eluent is further improved. The invention further provides a DNA elution method adopting the eluent for adsorbing the solid phase adsorbing material.

The invention relates to a drying process of a specific plant, which comprises the following steps of: 1. pretreatment: sorting prepared fresh pseudo-ginsengs; after separating and removing pruning openings, fibrous roots and flocking roots attached to the fresh pseudo-ginseng roots, and then obtaining main pseudo-ginseng roots; cleaning and removing surface attachments, leaching surface water and mincing by a mincer to obtain granules of 10-20 meshes; 2. pre-freezing: directly placing the granules into a pre-freezing chamber at minus 15-minus 20 DEG C and pre-freezing for 4-5 hours; 3. sublimation drying: spreading the pre-frozen granules on a table disk made of heat conducting materials, controlling the thickness of the spread material to be below 5cm and shifting to a vacuum drying chamber; after sealing, vacuumizing, maintaining internal vacuum pressure at 30-70Pa, setting the temperature of the table disk to be 40 DEG C, continuously supplying heat to the table disk and carrying out sublimation drying in vacuum for 5-7 hours till the table disk is heated to 40 DEG C; 4. analysis: maintaining the temperature of the table disk to be 40 DEG C and continuously supplying heat to the table disk and vacuumizing for 4-6 hours till the material is heated to 40 DEG C and material water is controlled to be below 3 percent; and 5. discharging and packaging.

The invention discloses a preparation method of an abalone's viscus protein peptide antioxidant and belongs to the technical field of deep processing of products and comprehensive utilization of by-products. According to the method, abalone's viscera are used as raw materials, after degreasing and deproteinization treatment, abalone's viscus antioxidant titanium powder is obtained through hot-water extraction, high-speed centrifugation and dialysis and then fully mixed with and dissolved in sodium alginate, the mixture is dropwise added to calcium chloride, gel beads are formed and cover protein peptide, and finally, the abalone's viscus protein peptide antioxidant is obtained through vacuum drying. Examples prove that the prepared antioxidant is safe, free of toxic and side effects, better in stability and high in antioxidant activity, cannot change properties under high temperature and humid conditions, has significant effects when being applied to preservation of meat foods and can prolong more than 80% of the shelf life of meat.

An electrochemical immunosensor, a preparation method and application thereof belong to the technical field of electrochemical immunosensors. In order to solve the problems of poor specificity, low sensitivity, long detection cycle and impossibility of in-situ online detection and monitoring of aflatoxin B1, the invention provides an electrochemical immunosensor, a preparation method and application thereof. The preparation method of the electrochemical immunosensor comprises the following steps of (1) preparing carbon fibers and gold nanoparticles modified gold electrodes; (2) immobilizing aflatoxin antibodies; and (3) blocking non-specific binding sites by BSA to obtain a standard curve, wherein y=0.1296+0.03774x, the linear correlation coefficient R2=0.98858, and the linear detection range of the curve is 0.01-1000ng/mL. The sensor provided by the invention can measure the content of aflatoxin B1 at a small range of voltage of about 0.11V or at an exact voltage.

A cultivation method of mesenchymal stem cell secretin comprises the following steps: S1, taking a mesenchymal stem cell and adding into a bioreactor for cultivation, obtaining a target amount, and washing mesenchymal stem cells in the bioreactor with a double-antibody-containing PBS buffer solution;S2, adding a complete medium I and the double-antibody-containing PBS buffer solution into the bioreactor to continue cultivation; S3, collecting supernate in the bioreactor, adding a PCH flocculating agent into the supernate, and precipitating protein in the supernate; S4,putting a protein precipitate obtained in S3 into a semi-permeable membrane, and using deionized water to repeatedly wash the protein precipitate in the semi-permeable membrane; S5, utilizing the deionized water to load a mesenchymal container with protein, and obtaining a protein liquid as the mesenchymal stem cell secretin. The cultivation method not only can effectively improve the space utilization rate and reduce the usage amount of a nutrient medium, but also improves the protein purity and reduces the loss rate.

The invention discloses a horizontal baking oven for a peanut oil press. The horizontal baking oven comprises a thermal-insulation shell and a baking rotating cylinder, wherein a ceramic layer is arranged on the inner wall of the baking rotating cylinder; two ends of the baking rotating cylinder are rotatably connected with two ends of the thermal-insulation shell; the baking rotating cylinder isdriven to rotate by a transmission motor; a combustion chamber is reserved between the thermal-insulation shell and the baking rotating cylinder; refractory bricks provided with combustors I are arranged at the bottom of the combustion chamber; a feeding pipe is arranged at the front end of the baking rotating cylinder, and a discharging pipe is arranged at the rear end of the baking rotating cylinder; an air outlet pipe communicated with the baking rotating cylinder is arranged on the baking rotating cylinder; and ceramic scraping plates are uniformly arranged on the ceramic layer on the inner wall of the baking rotating cylinder. The baking rotating cylinder is used for rotatably baking, and the whole peanut kernel is uniformly turned over by virtue of the ceramic scraping plates, so that the moisture is effectively removed, and the baking standard is uniform. By utilizing the ceramic layer and the ceramic scraping plates on the inner wall of the baking rotating cylinder, high-temperature clinkering can be effectively prevented, and the baking effect is qualitatively improved. The ceramic has good high-temperature stability, so that the baked peanut kernel does not have peculiarsmell and is environment-friendly, sanitary and easy to clean.

The invention discloses a sliding type piezoelectric valve used for discharging particle-contained liquid. The sliding type piezoelectric valve comprises a valve system and a power system, wherein a liquid feeding pipeline is arranged in the valve body of the valve system; the lower end of the liquid feeding pipeline is provided with a syringe needle; the inner cavity liquid outlet of the liquid feeding pipeline is arranged by corresponding to the liquid inlet of a syringe needle infusion tube; a sliding block is arranged between the inner cavity liquid outlet of the liquid feeding pipeline and the liquid inlet of the syringe needle infusion tube; the sliding block is provided with a pipeline; the pipeline on the sliding block can correspond to the inner cavity liquid outlet of the liquid feeding pipeline and the liquid inlet of the syringe needle infusion tube; the right end of the sliding block is provided with a first push rod; the left end of the sliding block is provided with a second push rod; the sliding block is driven by the first push rod and the second push rod to slide in the left and right directions; the power system comprises a driving power supply, a piezoelectric stacker and a compression rod. The sliding type piezoelectric valve has the beneficial effects of high colloid adaptation, small noise, small abrasion, long service life, convenience in regulation and few regulation parameters, and the sliding type piezoelectric valve is a discharging device which does not damage tiny granular substances.

The invention discloses an extracellular matrix freeze-drying powder. The extracellular matrix freeze-drying powder is prepared by a method with the following steps of (1) selecting animal tissues, degreasing, removing cells, and cleaning, so as to obtain a crude extract of the extracellular matrix; (2) extracting the crude extract of the extracellular matrix to obtain a hydrolysate, centrifugingor filtering the hydrolysate, and treating a supernatant or filtrate by a dialyzing method, an organic settling method or a salt settling method, so as to obtain the extract of the extracellular matrix; (3) filtering or centrifuging the extract of the extracellular matrix, mixing the filtrate or supernatant and monosaccharide or disaccharide, and freeze-drying, so as to obtain the extracellular matrix freeze-drying powder. The extracellular matrix freeze-drying powder has the advantages that the multiple active matters of polytype collagen, proteoglycan, growth factors and the like in the animal tissues are reserved; the multiple biological activities are realized, the biocompatibility is good, and the tissue regeneration is promoted; the anti-oxidizing, repair and wrinkle-resisting effects are realized, and the proliferation, transfer and differentiation of the cells are induced; the extracellular matrix freeze-drying powder can be used as the structure support, and the filling function is realized.

The invention discloses a recombinant protein photosensitizer for targeting an epidermal growth factor receptor and a preparation method of the recombinant protein photosensitizer, relating to the field of a genetic engineering technology and an antitumor biological medicine. A recombinant protein disclosed by the invention sequentially comprises an epidermal growth factor (EGF) interference sequence (20 amino acids of 1-20), a linker protein sequence (15 amino acid sequences of 21-35), a phycocyanin alpha sub-unit sequence (159 amino acid sequences of 36-194), and a 6*His-containing tag sequence (6 amino acid sequences of 195-200) from an end N to an end C. The recombinant protein is capable of directly targeting the tumor cell surface highly expressing the EGFR, and expressing a stronger effect of photoinduced-killing tumor cells through 630-nm laser irradiation for 20 minutes, and the inhibition rate reaches 95 percent; the recombinant protein is high in expression quantity, easy to purify, stable in performance, free from containing an organic solvent, and good in targeting, and is a brand-new fusion protein photosensitizer.

The invention provides a targeting recombinant protein photosensitizer and a preparation method thereof. The recombinant protein comprises, from N-terminal to C-terminal, epidermal growth factor (EGF) interference sequence (20 amino acids in total from 1 to 20)-linker protein sequence (15 amino acids in total from 21 to 35)-allophycocyanin alpha-subunit sequence (158 amino acids in total from 36 to 193)-6*His tag sequence (6 amino acids in total from 194 to 199) in sequence. The protein can be directly targeted to surface of tumor cells with high-EGFR expression level, and the protein shows strong photoinduced tumor cell killing effect after laser radiation at 650 nm for 20 min, with inhibitory rate of up to 100%. The protein has high expression level, stable performance and good targeting property, is easy for purification, does not contain organic solvent, and is a brand new fusion protein photosensitizer.

The invention discloses a production method of instant pot-stewed pig's trotters. The production method comprises the following steps of firstly cleaning raw pig's trotters, removing hair with rosin glycerin ester, then performing freeze-drying, then immersing the dried pig's trotters into enzymolysis juice for enzymolysis, then cooking the pig's trotters after enzymolysis in brine so that the cooked pig's trotters are tasteful, and finally performing antiseptic treatment and packaging. The instant pot-stewed pig's trotters produced by the method have the advantages of being safe to eat, easy to digest, unique in taste, not easy to deteriorate and the like.

An object of the present invention is to provide a first aid carrier for a cold area with temperature regulation. The invention provides a first aid carrier for a cold area with temperature regulation, which has the following characteristics: a first-aid bag body made of oxford cloth or canvas; and a cover having one side fixedly connected with the first-aid bag body, and the other three sides detachably connected with the body of the first aid kit through the opening and closing magic sticker, wherein a temperature regulating module area is used for regulating and heating the medicines placedin the temperature regulating module area, the thermostat module area has an insulating layer attached to the inner wall of the thermostat module area, a temperature regulating unit, and a power supply supplying power to the temperature regulating unit, the temperature regulating unit is composed of a temperature sensing part, a heating part and a temperature regulating button, wherein the temperature regulating button is used for medical personnel to select the required temperature range, the temperature sensing part induces the temperature in the temperature regulating module area, and thetemperature controlling part controls the heating part to heat according to the sensed temperature.