Coaxial electrospinning process of fibroin-polycaprolactone bi-component ultrafine fiber

A technology of coaxial electrospinning and polycaprolactone, applied in the direction of cellulose/protein conjugated artificial filament, fiber treatment, filament/thread forming, etc., can solve the problem of increasing the rejection probability of human organs and affecting the composite nanomaterials Fiber stability and other issues, to achieve the effect of improving coverage, simple method, and increasing stability

Inactive Publication Date: 2015-10-07
李松群
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The silk fibroin-polycaprolactone two-component ultrafine fiber under the existing technology is quantitatively detected by the above-mentioned electron spectrum, and the coating rate is generally 25-30%, which increases the rejection probability of human organs and greatly affects the composite nanofiber. Stability of fibers used in tissue engineering

Method used

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  • Coaxial electrospinning process of fibroin-polycaprolactone bi-component ultrafine fiber

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Embodiment 1

[0019] The invention discloses a coaxial electrospinning process of silk fibroin-polycaprolactone bicomponent ultrafine fibers, which comprises the following steps:

[0020] S1: Degumming silkworm silk in 0.06% sodium carbonate solution at 98-100°C for 3 times, 30 minutes each time, then washing with deionized water for 3 times, stretching and drying in an oven at 55°C for 3 hours Obtain thick silk fibroin after drying;

[0021] According to the lithium bromide solution that every 15g silk fibroin is equipped with 100ml 9.3mol / L, above-mentioned crude silk fibroin protein is dissolved in lithium bromide solution, forms the mixed solution of silk fibroin and lithium bromide;

[0022] Dialyze the above mixed solution of silk fibroin and lithium bromide with deionized water to obtain a high-purity silkworm fibroin protein solution, then place it in a polyethylene tray and dry it at 20°C for 2 hours to make a high-purity silk fibroin film;

[0023] Dissolving the above-mentioned ...

Embodiment 2

[0029] The invention discloses a coaxial electrospinning process of silk fibroin-polycaprolactone bicomponent ultrafine fibers, which comprises the following steps:

[0030] S1: Degumming silkworm silk in 0.06% sodium carbonate solution at 98-100°C for 2 times, 30 minutes each time, then washing with deionized water for 5 times, stretching and drying in an oven at 45°C for 0.5 hours Obtain thick silk fibroin after drying;

[0031]According to the lithium bromide solution that every 15g silk fibroin is equipped with 100ml 9.3mol / L, above-mentioned crude silk fibroin protein is dissolved in lithium bromide solution, forms the mixed solution of silk fibroin and lithium bromide;

[0032] The above mixed solution of silk fibroin and lithium bromide was dialyzed with deionized water to obtain a pure silkworm fibroin protein solution, and then placed in a polyethylene tray and dried at 10°C for 4 hours to form a high-purity silk fibroin film;

[0033] Dissolving the above-mentioned ...

Embodiment 3

[0038] The invention discloses a coaxial electrospinning process of silk fibroin-polycaprolactone bicomponent ultrafine fibers, which comprises the following steps;

[0039] S1: Degumming silkworm silk in 0.06% sodium carbonate solution at 98-100°C for 5 times, 30 minutes each time, then washing with deionized water for 2 times, stretching and drying in an oven at 60°C for 4 hours Obtain thick silk fibroin after drying;

[0040] According to the lithium bromide solution that every 15g silk fibroin is equipped with 100ml 9.3mol / L, above-mentioned crude silk fibroin protein is dissolved in lithium bromide solution, forms the mixed solution of silk fibroin and lithium bromide;

[0041] The above mixed solution of silk fibroin and lithium bromide was dialyzed with deionized water to obtain a pure silkworm fibroin protein solution, and then placed in a polyethylene tray and dried at 35°C for 0.5h to form a high-purity silk fibroin film;

[0042] Dissolving the above-mentioned high...

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Abstract

The invention discloses a coaxial electrospinning process of a fibroin-polycaprolactone bi-component ultrafine fiber. By optimizing the inner layer polycaprolactone solution concentration and the solvent component proportion, an inner layer polycaprolactone spinning solution and an outer layer fibroin spinning solution almost volatilize synchronously in a synchronous stretching process, and the incidence rate of coating rate decline caused by outer layer breakage due to earlier stretch stop than the inner layer can be reduced. The process provided by the invention employs the laboratory optimized concentration parameters corresponding to the inner layer solution and the outer layer solution, makes preliminary adjustment for almost synchronous volatilization of the inner layer and the outer layer, then adjusts the dosage of the dichloromethane solvent in the mixed solvent of the inner layer solution to regulate the solvent volatilization speed of the inner layer solution, and makes second fine adjustment for almost synchronous volatilization of the inner layer and the outer layer. The process provided by the invention has the advantages of simple operation method and easy realization, greatly improves the coating rate of the fibroin-polycaprolactone bi-component ultrafine fiber, and increases the stability of the fiber during application in bionic engineering.

Description

technical field [0001] The invention relates to an electrostatic spinning process, in particular to a coaxial electrostatic spinning process. Background technique [0002] The use of tissue engineering to construct biologically active implants in vitro and implant them in vivo to replace and repair human diseased and defective tissues and organs is one of the common cutting-edge research hotspots in materials science, engineering and life sciences. Among them, tissue engineering scaffold is the basic structure of tissue engineering tissue, which should have the structure and biological functions of natural extracellular matrix to support cell adhesion, growth and proliferation, and the degradation rate should match the tissue regeneration. From a biological point of view, almost all tissues and organs of the human body have the form and structure of nanofibers. Therefore, biomimetic tissue engineering scaffolds should be constructed and designed with nanofibers, so as to im...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01F8/02D01F8/14D01D5/00
Inventor 李松群
Owner 李松群
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