125 results about "Protein protein" patented technology

The invention discloses a method for preparing feed proteins by utilizing alcohol waste liquor and crop straws, aiming at the current situations that the feed availability of the crop straws is low and the alcohol waste liquor causes severe pollution. The requirements on straw integrated utilization and stock farming feed proteins and the objective needs on innocent treatment and utilization of the alcohol waste liquor are met. The feed protein product which is high in cellulose and xylan degradation degree and high in high-quality protein content is prepared by adopting a process for performing ammoniation, composite enzymatic hydrolysis and multi-strain three-step solid state fermentation, and the product is enriched in probiotics, vitamins, enzymes, amino acids, oligopeptides, high-quality proteins and other nutritional ingredients and has the advantages of promoting growth and development, improving the feed utilization rate, enhancing the immunity, improving the intestinal microecology and preventing diseases. Moreover, feed recycling high-value utilization of the crop straws and the innocent treatment and recycling of the alcohol waste liquor are realized, and the method has wide application prospects.

The present disclosure relates to protein-starch compositions. The disclosure also relates to processes for preparing the protein-starch compositions. Further, the disclosure relates to uses of the protein-starch compositions in the preparation of adhesives or binders. Further, the disclosure relates to adhesive formulations that include protein-starch containing compositions and to paper products resulting from the processes herein.

The invention relates to a low sugar or sugar free concentrated polyphenolic extract comprising at least about 30% polyphenols (w / w) and a protein-polyphenol aggregate matrix comprising at least about 15% polyphenols (w / w). The invention further relates to methods of producing a low sugar or sugar free concentrated polyphenolic extract comprising (a) extracting a low sugar or sugar free plant tissue with an aqueous solvent to produce an extract having an aqueous portion and a solids portion; (b) filtering the extract to separate the aqueous portion from the solids portion; and (c) reducing the volume of the separated aqueous portion, and methods of producing a protein-polyphenol aggregate matrix comprising about 1% to about 40% polyphenols (w / w) using the low sugar or sugar free concentrated polyphenolic extract of the invention.

The invention discloses a method for quickly detecting protein and amino acid in rapeseeds. The method comprises the following steps of: A, drying harvested rapeseeds in a drying oven, controlling the humidity for drying at 103 and 108 DEG C, controlling the time between 3 and 5 hours, and then cooling the rapeseeds to the room temperature; B, putting the cooled rapeseeds into a sample tray of a near infrared instrument to scan a near infrared spectrum, and repeating the sample loading for 2 to 4 times each time; C, averaging the spectrums measured by 2 to 4 times of a sample to calculate the average spectrum; and D, substituting the average spectrum into a model to calculating the content of crude protein and the amino acid of the sample. The method is easy to implement, is simple and convenient to operate, and has simple pretreatment of the sample. For a rapeseed manufacturer, the harvested rapeseeds are quickly measured at a certain temperature during a certain time. The method is quick and causes no damages. The acquisition time of the near infrared spectrum is very short, and the model calculation time can be basically ignored. By using the method, a plurality of components can be measured at the same time, and the content of the crude protein and the content of other amino acid in the rapeseeds can be measured at the same time.

Different from the conventional method for purifying human serum albumin from human plasma, the invention provides a simple method for separating and purifying the human serum albumin from fermentation liquor or a mammal cell culture solution. By an immobilized metal affinity chromatography (IMAC) step, the method is suitable for purifying recombinant proteins at various salt concentrations, fermentation supernate at high salt concentration is allowed to be directly loaded, the total volume of loading buffer is effectively reduced and the cost is reduced. The method is stable and high-efficiency, and is suitable for laboratories, pilot scale tests and industrial production scale-up.

The invention belongs to the technical fields of molecular biology, genomics and biotechnology, and particularly relates to a transposase-antibody binding protein fusion protein and preparation and application thereof. The fusion protein comprises a transposase portion, a linker peptide portion, and a portion capable of binding to an Fc segment of an antibody, and thus, the fusion protein has botha transposition function and an antibody Fc segment binding function. The fusion protein is used to create a new in-vivo protein-chromatin DNA interaction studying method, namely chromatin-transposition-sequencing (ChT-Seq) method. Compared with traditional ChIP-Seq, the ChT-Seq method has the following advantages: fewer steps, high efficiency, saving, good repeatability, less required sample DNA, and greatly-reduced sample and information lost in the process. The method is a powerful tool for studying in-vivo protein-chromatin DNA interaction, and has great significance for research on generegulation and epigenetics and the like.

The invention discloses an orange peel feed production method which comprises the following steps: washing fresh fruits, peeling off the fresh fruits, grinding and crushing, stirring and mixing, fermenting in a fermentation pond, performing degumming treatment, performing filter-press dehydration, mixing and stirring, performing secondary fermentation, performing aging treatment, blending and using, and the like. The technological process is reasonable, the processing equipment is simple, a high-energy-consumption drying process is not needed; as the fresh fruits are washed with pectinase and an acetic acid solution, are degummed, are fermented with yeast for multiple times and subjected to the action of bacillus subtilis, microelements such as phosphorus, calcium, iron and zinc, amino acid, vitamin C, and physiologically active ingredients such as, flavonoid and carotenoid can be preserved, and the content of protein in orange peel residue is up to 5-6%. By adopting the orange peel feed production method, the problem that the orange peel resource is wasted, the environment is polluted and the like are effectively solved, and compared with ordinary feed, the orange peel feed is good in palatability, low in seasonal febrile disease rate, consistent in growth period and good in economic benefit.

The present invention provides compositions and methods for improving nucleic acid or protein recovery from fixed biological samples.

The invention provides a method for predicting potential sensitization in protein. The method includes: firstly, making a training positive set and a training negative set; secondly, encoding various attributes of protein to build characteristic vectors; thirdly, sorting characteristics by maximum correlation and minimum redundancy methods, and selecting optimal characteristics by progressive characteristic selection method; and fourthly, counting and analyzing the selected characteristics, and giving a report of characteristic results evidently related to the sensitization in protein. The potential sensitization of the protein can be predicted effectively, the method is more accurate than the existing computational biology prediction methods, protein characteristics related to the sensitization of the protein can be analyzed effectively, and the method is significant to prediction of allergens, and study on the sensitization of the protein.

The invention discloses a production method of selenium-enriched bamboo shoots. The production method comprises adding organic acid and sucrose into a sodium selenite aqueous solution for reaction to obtain a selenium-enriched organic acid sucrose solution; adding the pure water into the selenium-enriched organic acid sucrose solution for dilution, controlling the selenium concentration into 20 to 40 mg every kg and obtaining a selenium-enriched organic acid sucrose injection; injecting 20 to 40 ml of selenium-enriched organic acid sucrose injection into a bamboo shoot body through a syringe in an inclined mode and sealing an injection hole through the beewax; harvesting when bamboo shoots are 20 to 30 cm higher than the ground after 3 to 7 days of injection to obtain the selenium-enriched bamboo shoots. According to the production method of the selenium-enriched bamboo shoots, the obtained selenium-enriched bamboo shoots are high in nutritional value, slightly sweet and free of bitter, nutrients such as the rich protein and the amino acid, particularly the trace element selenium, are contained in the selenium-enriched bamboo shoots, the good antioxidant effect such as the cosmetic, anti-cancer and anti-aging effect is achieved, and the selenium-enriched bamboo shoots can be utilized as antioxidants or health food development raw materials.

The invention relates to a method for quickly and accurately discovering, identifying and preparing a proteolysis provenance antibacterial peptide, belonging to the field of the preparation and the purification of biologically-active peptides. According to the method, for example, Jatropha curcas seed protein, an ingredient containing the antibacterial peptide is prepared through hydrolysis by using a variety of proteases, and the antibacterial peptide is quickly discovered, identified and prepared and purified by using a technology which combines cell membrane affinity chromatography with a high-performance liquid phase fingerprint atlas and a mass spectrum. According to the method, the separation and the purification are divided into two steps, i.e. affinity extraction and BR-HPLC / MS / MS separation and identification, the traditional steps (usually involving 5-7 steps, such as ultrafiltration, anion and cation exchange, macroporous resin adsorption, desalting, gel chromatography and BR-HPLC) for purifying the antibacterial peptide are greatly shortened, and all of the obtained samples reach chromatographic purity. Visibly, a way with simplicity, convenience and fastness in operation, for the large-scale preparation, the accurate discovery and the fast purification of biological active substances in less content, such as the antibacterial peptide, is provided due to the establishment of the method provided by the invention.

Methods for identifying a cancer patient, such as a breast cancer patient, suitable for treatment with a 4-anilinoquinazoline kinase inhibitor, such as lapatinib, and an AKT inhibitor, comprising detecting modulated expression of HER2 (ERBB2) and SASH1 or protein encoded thereof and detecting PIK3CA mutation status. High levels of expression in HER2 and high levels of SASH1 and / or positive PIK3CA mutation status indicate a patient that is suitable for treatment with a 4-anilinoquinazoline kinase inhibitor, such as lapatinib and an AKT inhibitor.

The invention discloses a wheat protein regenerated cellulose fiber and a preparation method thereof. The cracking strength of the wheat protein regenerated cellulose fiber is 3.1-3.6 cN / dtex, the wetbreaking strength is 1.7-2.0 cN / dtex, the dry breaking elongation is 13-15%, the linear density is 1.67-4.44 dtex, ad the protein content is 1-8%. Protein used in the method is wheat protein micro-powder processed through a special method rather than hydrolyzed protein, so that a massive loss of hydrolyzed protein during spinning forming is avoided. The prepared regenerated cellulose fiber is relatively good in mechanical property and flexibility, and textiles prepared from the fiber have relatively good wear-resisting strength, elasticity, wrinkle resistance and drapability. In addition, when wheat protein micro-powder blending viscose is prepared, wheat protein micro-powder dispersion liquid is added into a modified viscose stock solution through injection before spinning, and a whole spinning system is not contaminated.

A vector of the present invention has DNA encoding a protein or a product having the same effect as the protein, the protein containing an amino acid sequence from amino acid numbers 47 to 802 in SEQ. ID. NO:2. Expression of the DNA gives human chondroitin synthase. By using human chondroitin synthase, it is possible to produce a saccharide chain having a repeating disaccharide unit of chondroitin. The DNA or part thereof may be used as a probe for hybridization for the human chondroitin synthase.

The invention relates to a composite fiber containing wheat protein and a preparation method thereof; the preparation method of the fiber comprises the steps of preparing a wheat protein composite solution, and preparing cationic fibers, cross-linked wheat protein and an adsorption plant extract; no strong acids are used in the method when the protein and plant extract are adsorbed, all effectiveingredients are not damaged, and therefore, the prepared composite fiber has good properties; the fiber prepared through the method disclosed by the invention has the advantages of bright and soft luster, good moisture absorption and release and good softness, has high dry breaking strength, wet breaking strength and dry breaking elongation, and has good antibacterial effect, wherein the dry breaking strength is greater than 2.25 CN / dtex, the wet breaking strength is greater than 1.25 CN / dtex, the dry breaking elongation is greater than 9%, and the antibacterial rate is higher than 95%.

The present invention relates to a technical field of protein engineering for increasing the enzyme activity and thermal stability of human hyaluronidase, which is a hyaluronic acid-hydrolyzing enzyme, and relates to a hyaluronidase PH20 mutant or a fragment thereof, comprising: the substitution of at least one amino acid residue, among the amino acid sequence of the wild-type PH20 having SEQ ID NO: 1, at an alpha helix site and / or a site corresponding to a connection site thereof; and selectively, the additional deletion of an N-terminal amino acid residue and / or a C-terminal amino acid residue. Specifically, the present invention relates to a PH20 mutant and a fragment thereof, the wild-type PH20, having a sequence of SEQ ID NO: 1, comprising: the substitution of at least one residue selected from the group consisting of T341A, T341C, T341G, S343E, M345T, K349E, L353A, L354I, N356E and I361T; additionally, the substitution of an amino acid positioned at alpha helix site 8 and / or theconnection site of alpha helix site 7 and alpha helix site 8; and the deletion of some amino acids of an N-terminal site and a C-terminal site.

Methods for preparation of molecularly imprinted polymers and their use for detection of proteins and / or polypeptides in a sample are disclosed. The methods of preparation are based on selecting from available data bases an amino acid sequence of a protein / polypeptide target molecule; cleaving the sequence in-silico with at least one cleaving agent, producing fragments with known composition; selecting at least one such fragment comprising a unique epitope; preparing a synthetic peptide representing the unique epitope; and preparing a molecularly imprinted polymer comprising specific binding sites for the synthetic peptide. For detection of the target protein in a sample, the same cleaving agent used for the in-silico cleavage is used to cleave the target protein to form the specific peptide fragments to which the MIP is specific.

A system and method that predicts whether a given protein-ligand pair is active or inactive, the ground-truth protein-ligand complex crystalline-structure similarity, and an associated bioactivity value. The system and method further produce 3-D visualizations of previously unknown protein-ligand pairs that show directly the importance assigned to protein-ligand interactions, the positive / negative-ness of the saliencies, and magnitude. Furthermore, the system and method make enhancements in the art by accurately predicting protein-ligand pair bioactivity from decoupled models, removing the need for docking simulations, as well as restricting attention of the machine learning between protein and ligand atoms only.

The present invention relates generally to compositions and methods for reprogramming a primate somatic cell to a higher potency level. Specifically, the invention includes compositions which comprise a recombinant polypeptide that is a potency-determining factor and methods of reprogramming a primate somatic cell to a higher potency level under conditions that allow sufficient amount of the polypeptide delivered into the primate somatic cell.

The invention relates to a deep learning prediction method of target-ligand binding affinity based on a gated attention mechanism, and belongs to the field of computer-aided drug design technology and biology and pharmacoinformatics. The deep learning model starts from an SMILES character string of a ligand and an amino acid sequence of a protein, and then is converted into a ligand matrix and a protein matrix. The ligand matrix is sent to a full connection layer and an attention layer based on gate enhancement for feature extraction, and the protein matrix is sent to a one-dimensional convolution layer and a maximum pooling layer and then sent to the attention layer based on gate enhancement. Finally, the processing characteristics of the ligand matrix are polymerized by adding matrix rows, and the same process is performed on the protein matrix, and then the two are spliced together and sent to a subsequent full connection layer to predict the probability of high / low binding affinity of the protein-ligand complex. According to the method, the time and cost related to experimental analysis are effectively reduced, and the efficiency of drug design and virtual screening is improved.

The invention belongs to the field of calcium tablet preparation and particularly relates to a calcium tablet special for pregnant women and wet nurses and a preparation method thereof. The calcium tablet is prepared from the materials in parts by weight: 340 to 360 parts of calcium carbonate, 1.4 to 1.8 parts of casein phosphopeptides, 670 to 690 parts of glucose, 15 parts of citric acid, 3 parts of purified water, 5.1 parts of edible alcohol, 85 to 95 parts of dextrin, 40 to 50 parts of maltodextrin, 11 to 13 parts of magnesium stearate, 2 to 3 parts of sweet orange essence and 50 to 55 parts of complex mushroom powder; the complex mushroom powder is prepared from hericium, shiitake mushroom, straw mushroom, edible fungus, tremella and needle mushroom according to a certain mass ratio. The complex mushroom powder contains rich protein, carbohydrates, vitamins and mineral substances such as calcium, magnesium, copper and zinc, can promote the quick absorption of the calcium, has blood replenishing and activating functions, can effectively replenish nutrition in pregnancy and after delivery, and provides nutrition needed for quick rehabilitation of the pregnant women and puerperae.

The invention relates to a method for preparing rennet casein by using cow's milk, comprising the following steps of: (1) pre-treating; (2) degreasing; (3) sterilizing; (4) acidifying; (5) curdling; (6) whey discharging; (7) washing with water; (8) dehydrating; and (9) drying and then crushing and screening to obtain a rennet casein product, wherein the water content of the obtained product is 6-12% while a protein content of the product is 85-95%. In the invention, a skimmed milk is obtained through separation; rennin is added to the skimmed milk to prepare a fresh curd after the skimmed milk is sterilized and acidified; and the fresh curd is washed with water, dehydrated, dried and crushed to obtain the rennet casein. The method has the advantages of reliable operation, less pollution, high purity of the finished product and good functional character, is suitable for manufacturing multiple foods and is beneficial for large scale production.

The invention provides Degronimers that have E3 Ubiquitin Ligase targeting moieties (Degrons) that can be linked to a targeting ligand for a protein that has been selected for in vivo degradation, andmethods of use and compositions thereof as well as methods for their preparation. The invention also provides Degrons that can be used to treat disorders mediated by cereblon or an Ikaros family protein, and methods of use and compositions thereof as well as methods for their preparation.

The invention relates to a candida utilis strain UCY-11 and application thereof in preparation of fermented hybrid paper mulberry feed. The candida utilis strain UCY-11 can effectively solve the problems of degrading macromolecular proteins contained in the feed, increasing the content of crude proteins and peptides in the crude proteins, reducing the content of anti-nutritional factors such as cellulose and lignin, improving the nutritional value of the feed and meeting the actual needs of breeding animal husbandry when the fermented hybrid paper mulberry feed is prepared by using the strain.The candida utilis strain UCY-11 is obtained by an ultraviolet mutagenesis technology, and the strain is used for fermenting the hybrid paper mulberry feed, so that the content of the crude proteinsand the peptides in the crude proteins can be effectively increased, the nutritional quality of the hybrid paper mulberry feed is improved, and the utilization efficiency of animals on the hybrid paper mulberry feed is improved; and meanwhile, the intestinal microecological balance of the animals is improved, the development of animal health and breeding industry is promoted, the needs of people on animal proteins in life are met, and the candida utilis strain UCY-11 has remarkable economic and social benefits.

The invention provides methods and systems of determining biopolymer profiles and correlations between structural units (residues) of a biopolymer based on sampling of the conformational space available to the molecule. The correlations between these structural units can further be used to find networks within a biopolymer such as the coupled residue networks in a protein. The invention also provides for designing and engineering biopolymers including polypeptides, nucleic acids and carbohydrates using the information derived from the conformation clustering and subsequent methods described herein.

Methods and compositions for producing variants of a polypeptide are disclosed. Variants are generated using modifying enzymes specifically targeted to the polypeptide through the interaction of a T7 polymerase with a T7 polymerase promoter.

A method of enzyme-based processing of plant-based materials includes steps of providing a bulk amount of a plant-based material having a substantial amount of the cell walls in a physically-intact condition; providing an enzyme broth having an enzyme capable of breaking down the cell walls in the physically-intact condition; combining the bulk amount of the plant-based material with the enzyme broth; allowing the enzyme capable of breaking down the cell walls in the physically-intact condition to break down at least a portion of the cell walls in the physically-intact condition to thereby produce intact oil bodies, hydrolyzed carbohydrates, and intact protein bodies; collecting a first product including the intact oil bodies; collecting a second product including the hydrolyzed carbohydrates; and collecting a third product including the intact protein bodies.

The present invention relates to a composition for preventing or treating a muscle disorder, which includes a chrysanthemum extract or 3,5-dicaffeoylquinic acid as an active ingredient, and specifically, it may be used to reduce mRNA expression of atrogin-1 and MuRF1, which are main biomarkers involved in muscle protein degradation and increase mRNA expression of the mTOR protein, which is a main biomarker involved in muscle protein formation, and myogenin and MyoD, which are biomarkers related to muscle differentiation, thereby reducing muscle loss, and thus the chrysanthemum extract or 3,5-dicaffeoylquinic acid can be used in prevention and treatment of a muscle disorder, or improvement in muscle function. In addition, the chrysanthemum extract or 3,5-dicaffeoylquinic acid increases the activity of SIRT1 and PGC-1α, which are the main biomarkers involved in exercise performance, thereby excellently enhancing exercise performance. In addition, the present invention is a natural substance and may be safely used without a side effect, and therefore may be used as a medication, food or a cosmetic.

Described is a low voltage, pulsed electrical stimulation device for controlling expression of klotho, a useful protein, by tissues. Also described are methods of enhancing expression of klotho in cells.

This invention relates to a truncated dysferlin nucleic acid and protein, vectors (e.g., adeno-associated virus vectors) comprising the nucleic acid and methods of using the same for delivery of dysferlin to a cell or a subject and treating dysferlinopathy.