Chondroitin synthase

Inactive Publication Date: 2005-03-03
OTSUKA CHEM CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

0151] A probe of the present invention is expected to be a useful tool for examining biological functions of chondroitin sulfate. This is because chondroitin sulfate widely

Problems solved by technology

However, cDNA cloning of those enzymes has not been performed yet b

Method used

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example 1

[0153] (1) In Silico Cloning of Novel Human Glycosyltransferase cDNA

[0154] Screening of HUGE protein database at Kazusa DNA Research Institute (in Chiba Prefecture; http: / / www.kazusa.or.jp / huge / ) was conducted by the keywords "one transmembrane domain" and "galactosyltransferase family". As a result of this, one clone (KIAA0990; GenBank.TM. accession number AB023207) was identified. An analysis of a nucleotide sequence of this clone revealed that this clone includes (i) a 5'-untranslated region of 494 bp, (ii) a single open reading frame of 2406 bp coding for a protein of 802 amino acids with three potential N-glycosylation sites (marked with asterisks in FIG. 1), and (iii) a 31-untranslated region of about 1.7 kb with a presumptive polyadenylation signal. The nucleotide sequence and an amino acid sequence deduced from the same are shown in SEQ. ID. NO: 1, whereas only the amino acid sequence is shown in SEQ. ID. NO: 2.

[0155] The clone was acquired from Kazusa DNA Research Institute...

example 2

[0175] A commercially-available human 12-lane multiple tissue Northern blot (Clontech) membrane was used for analysis. To each lane, 1 .mu.g of a polyadenylated RNA was applied. The membrane was probed with a gel-purified and radiolabeled (>1.times.10.sup.9 cpm / .mu.g) 0.84 kb chondroitin-synthase-specific fragment corresponding to nucleotides 631-1469 of the KIAA0990 cDNA (GenBank.TM. accession number AB023207).

[0176] As a result, a single band of up to 5.0 kb was demonstrated for all human tissues, at least in this analysis (FIG. 4). The degree of the expression of the chondroitin synthase gene which is prevalent in human tissues varied with the types of human tissues. Notably, a particularly strong expression of the mRNA was observed in the placenta. The expressions observed in the spleen, lung, and peripheral blood leukocytes were also strong but not as much as that of the placenta. This result corresponds to an observation that chondroitin sulfate proteoglycans are distributed a...

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Abstract

A vector of the present invention has DNA encoding a protein or a product having the same effect as the protein, the protein containing an amino acid sequence from amino acid numbers 47 to 802 in SEQ. ID. NO:2. Expression of the DNA gives human chondroitin synthase. By using human chondroitin synthase, it is possible to produce a saccharide chain having a repeating disaccharide unit of chondroitin. The DNA or part thereof may be used as a probe for hybridization for the human chondroitin synthase.

Description

[0001] The present invention relates to (a) a vector having DNA encoding chondroitin synthase, (b) a method of producing chondroitin synthase, (c) a method of producing a saccharide chain having a repeating disaccharide unit of chondroitin, and (d) a probe for hybridization of chondroitin synthase.[0002] Chondroitin sulfate, which is a kind of glycosaminoglycan (GAG), exists as a proteoglycan on cell surfaces and in an extra cellular matrix. Chondroitin sulfate draws attention because Chondroitin sulfate plays an important role in neural network formation in the developing mammalian brain (Arch. Biochem. Biophys. 374, 24-34 (2000); Trends Glycosci, Glycotechnol. 12, 321-349 (2000)).[0003] Chondroitin sulfate has a straight-chained polymer structure having a repeating disaccharide unit having a glucuronic acid residue (GlcUA) and an N-acetylgalactosamine residue (GalNAc). A serine residue in a core protein is covalent-bonded with chondroitin sulfate via 4-saccharide structure (GlcUA....

Claims

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Application Information

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IPC IPC(8): C12N1/15C12N1/19C12N15/09C12N1/21C12N5/10C12N9/10C12P19/26C12P19/28C12Q1/68
CPCC12P19/26C12N9/1051
Inventor SUGAHARA, KAZUYUKIKITAGAWA, HIROSHI
Owner OTSUKA CHEM CO LTD
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