303 results about "Cellular secretion" patented technology

Methods, systems, and devices for enumerating the number of cells present in each event for cell-secretion based sorting applications are described herein. The cell secretion applications use carrier to encapsulate cells and collect the biomolecules they secrete. The carriers are then sorted using flow based particle sorting. The total number of cells present in a carrier is very important for several applications. A convolutional neural network is used to count the number of cells present from a brightfield image, and outputs the information to be used as part of the sort logic.

The invention relates to a method for separating and purifying polygonatum sibiricum oligosaccharide. According to the method, the polygonatum sibiricum polysaccharide and the filler or / and the filter aid are fully mixed and then separated and purified; wherein the filler is prepared from Sephadex G-15 (crosslinked glucose gel), DEAE-Sepharose CL-4B (agar gel) and Bio-Gel P-2 (polyacrylamide gel), the filter aid is prepared from kieselguhr, perlite, cellulose, activated carbon and red soil, and the method has the advantages that the Mwlt can be obviously improved; the yield of the 2KDa polygonatum sibiricum oligosaccharide is increased, and the effect of combined use of the filler and the filter aid is optimal. The addition of the filter aid can avoid the blockage of filler pores in the middle and later stages of elution, improve the elution performance of the filler on polysaccharide, and accelerate the elution and purification speed of the polygonatum kingianum oligosaccharide. Experiments show that the oligosaccharide can significantly enhance the proliferative activity and phagocytosis of macrophages, promote RAW264.7 cells to secrete TNF-alpha, IL-6, NO and other cell factors, and improve the cellular immunocompetence. The method has the characteristics of convenience, high efficiency and low cost, and has relatively high application value.

The present invention discloses a preparation method of Inonotus hispidus polysaccharide IHP-1aa: It is obtained by removing fat with ethanol, extracting with water and precipitating with ethanol, then removing proteins by the Sevage method, separating neutral polysaccharide components through a DEAE-52 cellulose column and a DEAE agarose gel column, and finally purifying with Sephadex G-100. The Inonotus hispidus polysaccharide has uniform purity and clear composition, and is a heteropolysaccharide composed of glucose, galactose, mannose, fucose and methylgalactose, with a molecular weight of 26.9 kDa. In vitro experiments show that it has no adverse effects on normal cells, can significantly stimulate macrophages RAW264.7 to secrete NO, IL-6 and TNF-α, has good immunomodulatory activity, and can be used for the development of immunomodulatory products.

The invention provides a protein degradation targeting chimera based on G-quadruplex RNA (Ribonucleic Acid) as well as a preparation method and application of the protein degradation targeting chimera. The structural general formula of the protein degradation targeting chimera is G-L-E, wherein G is G quadruplex RNA specifically combined with mammal fragile X hypophrenia protein (FMRP), E is a ligand of micromolecule E3 ubiquitination ligase, and L is a connecting arm between the G quadruplex RNA and the ligand of the micromolecule E3 ubiquitination ligase. The protein degradation targeting chimera provided by the invention has the advantages that the stability in an in-vivo environment and the capability of entering cells are greatly improved, and the protein degradation targeting chimera also has the capabilities of efficiently degrading FMRP and changing a secretion mode of cancer cells, so that a lot of convenience is provided for the development of nucleic acid PROTAC drugs.

The invention belongs to the technical field of micro-fluidic chips, and discloses a hepatic lobule chip which comprises a single cell capture array chip, a micropore array chip and an antibody slide, a capture array on the single cell capture array chip and a micropore array on the micropore array chip are in a polygon shape, perfusion culture solution channels are arranged in the diagonal directions of all vertexes of the polygon, and the capture array and the micropore array are in one-to-one correspondence after being assembled. The liver lobule chip overcomes the dependence of research on spatial heterogeneity of liver cells on precise liver anatomical slices, avoids the defects that the liver slices are difficult to obtain and store, can directly reveal spatial distribution characteristics of secretion activity of the liver cells in the liver lobule, and becomes an ideal tool for spatial heterogeneity secretion of the liver cells. The invention further discloses application of the hepatic lobule chip in hepatic lobule spatial heterogeneity single cell secretion analysis, operation is easy, large experimental instruments are not needed, cost is low, and the hepatic lobule chip is easy to master.

The invention provides brain cell specific targeting delivery based on exosome, and the exosome secreted by 293T cells is used for engineering transformation, so that the capabilities of penetrating BBB and specifically targeting neurons are achieved. According to the present invention, the L1CAM is loaded on the exosome, the exosome is obtained by transfecting a 293T cell of a Lamp2b-L1CAM PCDH-HygroHA plasmid through a 293T-Lamp2b-L1CAM cell line, and the BBB cell-penetrating peptide 4F-T7 and the neuronal targeting protein L1CAM are loaded on the surface of the exosome so as to treat the neurodegenerative diseases such as the Alzheimer's disease and the Parkinson's disease.

The utility model provides a microfluidic electrical stimulation chip device and a system for promoting cells to secrete exosomes by using the device, the microfluidic electrical stimulation chip device comprises an upper substrate, a microfluidic electrical stimulation chip body and a lower substrate, a liquid inlet and a liquid outlet are etched or tapped on the upper substrate in a hollowed-out manner, an upper substrate electrode is embedded in the lower surface of the upper substrate, and a lower substrate electrode is embedded in the lower surface of the lower substrate. A micro-channel liquid inlet section, a micro-channel reaction section and a micro-channel liquid outlet section are engraved on the micro-fluidic electrical stimulation chip body, and a lower substrate electrode is embedded in the upper surface of the lower substrate; the system for promoting the cells to secrete the exosomes by using the device comprises a cell supply bioreactor, a power device, a micro-fluidic electrical stimulation chip device, a power supply device and a cell recovery bioreactor, and can realize on-line electrical stimulation treatment of the cells in a normal suspension culture process of the cells; cell suspensions flowing through a plurality of micro-channels in the micro-fluidic electrical stimulation chip are stimulated through electrical signals, cells are promoted to secrete exosomes, and the yield of the exosomes is increased.

A secretion analysis and sorting system where cell(s) are deposited into a carrier that will capture any secretions from the cell, and is small enough to be sorted using flow cytometry based cell sorting, is described herein. The use of an image classification workflow that identifies the event types present in a sample of carriers allows the user to select which event types they would like to purify, and then train a supervised classification system that will be used to make real time sort decisions to purify the carriers which contain cells that secrete the desired product. A flow cytometer is then able to be used to screen the individual carriers to see if each one has the desired secreted product.

The invention relates to the technical field of medical cosmetology, in particular to a porous hollow glass bead capable of slowly releasing collagen and application of the porous hollow glass bead. The porous hollow glass bead capable of slowly releasing the collagen comprises a porous hollow glass bead body, the outer wall of the porous hollow glass bead body is provided with a pore channel structure penetrating through the exterior and an inner cavity of the hollow glass bead body, and the pore channel and the inner cavity of the porous hollow glass bead body are filled with collagen gel. The compressive strength of the hollow glass beads is utilized to improve the mechanical supporting effect, the collagen gel is subjected to catalytic decomposition under the action of collagenase in a body so as to achieve the slow-release effect, the outer walls of the hollow glass beads slow down the reaction time through physical isolation, and then the slow-release time is prolonged; meanwhile, ions such as Si and Ca released by the hollow glass beads can promote cells to secrete collagen, the biological activity is improved, and the three beneficial effects of physical support, collagen slow release and biological safety are achieved.

The invention relates to the technical field of vaccines, and discloses a preparation method and application of an anti-tumor vaccine based on an autoantigen and a synthetic antigen. The anti-tumor vaccine comprises a tumor autoantigen and a synthetic antigen, wherein the tumor autoantigen is extracted from a tumor cell membrane, a tumor cell lysate, a tumor cell secretion, an exosome antigen or an antigen released by a tumor microenvironment; the synthetic antigen is selected from at least one of a tumor associated antigen, a tumor specific antigen, a personalized neoantigen, a pathogen antigen, a biotoxin, a biomolecule antigen and coding nucleic acid of a peptide type antigen in the antigens; the coding nucleic acid is DNA or mRNA. The vaccine molecule composed of the tumor autoantigen and the synthetic antigen is easy to prepare, has a strong anti-tumor immune effect and good biological safety, solves the problems that the traditional tumor autoantigen has no specific precise target spot and the synthetic antigen is single in immunogen, and has a wide application prospect.

The invention relates to application of a palmitoylation inhibitor in preparation of a medicine for treating spontaneous abortion. The palmitoylation inhibitor is 2-BP (2-Butyl Phosphate). The spontaneous abortion is recurrent abortion caused by unknown reasons. The 2-BP is used for promoting the dCD4 < + > T cells to secrete Th2 and Treg type cell factors. The 2-BP reduces embryo loss and promotes the tolerance phenotype of dCD4 + T cells, facilitating maternal-fetal immune tolerance. The invention provides an application of 2-BP in a medicine for treating spontaneous abortion. In particular, in-vitro experiments and animal experiments prove that 2-BP promotes Th2-type bias and Treg amplification of dCD4 + T cells, reduces embryo absorption and improves bad pregnancy outcome.

The invention relates to the technical field of health-care products, in particular to a health-care product rich in momordica charantia polypeptide and capable of regulating blood sugar metabolism and promoting insulin secretion, momordica charantia polypeptide can stimulate pancreatic beta cells to secrete insulin, and insulin is the only hormone capable of reducing blood sugar in a human body. The balsam pear polypeptide component in the balsam pear extract is specially used for reducing the blood sugar level and improving related health problems, the application of the balsam pear polypeptide in the health care product can effectively regulate the blood sugar metabolism of a human body and promote the secretion of insulin, and the balsam pear polypeptide stimulates pancreas islet beta cells to promote the secretion of insulin. In addition, the balsam pear polypeptide can improve the insulin resistance, increase the number of insulin receptors or enhance the activity of the insulin receptors, so that the body cells are more sensitive to the insulin, the uptake and utilization of glucose are promoted, and the abnormal increase of the blood glucose level is avoided.

The present invention relates to the new use of an exosome secreted by hepatocyte lineage cells differentiated via the directed induction of embryonic stem cells, and in particular relates to the use of the exosome in the preparation of a drug for treating liver diseases. The exosome is secreted by hepatocyte lineage cells differentiated via the directed induction of embryonic stem cells, and the hepatocyte lineage cells are hepatic progenitor cells and / or mature hepatocyte-like cells.

The invention belongs to the technical field of biotechnology and tissue engineering, and particularly relates to application of pilose antler bud cell secretions in preparation of anti-osteoporosis drugs. The pilose antler bud cell secreta is obtained by adopting gradient centrifugation, and cell experiments show that the pilose antler bud cell secreta provided by the invention has the effect of promoting osteogenic differentiation of cells; animal experiment results show that the cornu cervi pantotrichum bud cell secreta provided by the invention can significantly improve the bone mineral density, bone volume fraction, bone trabecula number, bone trabecula thickness, bone trabecula separation degree and cortical bone mineral density of mice, increase the bone trabecula area of primary osteogenesis and secondary osteogenesis centers of the mice, improve osteoporosis symptoms of the mice, and improve osteoporosis of the mice. The effect is obviously better than that of bone marrow mesenchymal stem cell secretions, part of indexes are equivalent to the treatment effect of diphosphates, and the antler bud cell secretions provided by the invention can be used for preparing medicines for preventing, relieving or treating osteoporosis.

The invention relates to the technical field of cell biology, in particular to application of a composition containing a mesenchymal stem cell secretor in pulmonary fibrosis. A systematic pharmacodynamic experiment verifies that the composition can remarkably reduce the content of a key fibrosis promoting factor TGF-beta1 in the blood of a pulmonary fibrosis model rat and effectively improve the blood-gas exchange function at the same time, and the blood oxygen saturation (SaO2) is remarkably increased, and the partial pressure of carbon dioxide (pCO2) is remarkably reduced. The improvement of the key indexes proves that the composition has a definite anti-fibrosis effect and a lung function protection effect, and a brand new treatment strategy is provided for pulmonary fibrosis diseases which lack special-effect treatment clinically at present. The technology not only has a remarkable clinical transformation value, but also develops an innovative research thought for the lung disease treatment field based on the action mechanism of the stem cell secretor, and has important scientific significance and market development prospect.

Systems, methods, compositions, and kits for measuring secreted factors from cells are disclosed herein, including those capable of determining single cell secretion activity and protein expression and / or gene expression simultaneously. Disclosed herein include solid supports comprising a plurality of capture probes capable of specifically binding to at least one of the plurality of secreted factors secreted by a single cell. Also disclosed herein include secreted factor-binding reagents capable of specifically binding to a secreted factor bound by a capture probe. A secreted factor-binding reagent can comprise a secreted factor-binding reagent specific oligonucleotide comprising a unique factor identifier sequence for the secreted factor-binding reagent.

The invention relates to a micro-fluidic chip for chemiluminescence imaging detection of single-cell secretions and a use method of the micro-fluidic chip. The micro-fluidic chip is composed of a single cell analysis layer and a glass substrate layer. The single cell analysis layer is prepared from polydimethylsiloxane and is provided with a capture channel, a sample introduction channel and a lantern-shaped microstructure array, the lantern-shaped microstructure comprises a small-size cell capture trap and a large-size chemiluminescence detection chamber, the small-size cell capture trap is connected with the capture channel, and the large-size chemiluminescence detection chamber is connected with the sample introduction channel. After a mixed solution of cells and a detection reagent is introduced into the capture channel, single cell capture is realized on the capture trap due to flow resistance difference; then, introducing air into the capture channel and the sample introduction channel to form a gas-in-liquid microcavity, and carrying out single cell isolation and incubation reaction; finally, the signal switch molecules and the substrate liquid are sequentially injected into the chemiluminescence detection chamber through the sample injection channel, and quantitative detection is conducted on the single-cell secretions according to chemiluminescence imaging signals.

The invention discloses a single traditional Chinese medicine powder spray for radically treating allergic rhinitis and preparation and application thereof.The single traditional Chinese medicine powder spray only contains gleditsia sinensis powder, is simple in composition and can temporarily stimulate epithelial cells to secrete normal nasal discharge to block and flush allergens, and nasal cavity flushing is achieved; the traditional Chinese medicine composition can change the local microenvironment of nasal mucosa and cut off the biological process of occurrence and development of allergic rhinitis, and enters the nasal mucosa to serve as a descaling agent, so that an IgE receptor Fc epsilon R1 on a sensitized mast cell membrane is separated from IgE and cannot be combined with allergens, and the mast cell cannot activate and release an allergic medium, so that the allergic rhinitis is radically treated; the preparation process of the single traditional Chinese medicine powder spray is simple and easy to operate, batch production can be realized, and after the saponin powder is processed by the alum, the astringency effect is remarkable, so that the clear water snot symptom of the allergic rhinitis can be improved; the nasal spray is used for treating allergic rhinitis, stimulates secretion of nasal discharge after being sprayed, and is beneficial to cleaning nasal cavities and improving nasal ventilation; the traditional Chinese medicine composition also has a sterilization function and can effectively inhibit bacterial and fungal infection induced by allergic rhinitis.

The invention belongs to the field of functional polypeptide preparation, and particularly relates to euphausia superba peptide and application thereof in preparation of immunomodulatory drugs. The euphausia superba peptide is prepared from the following peptide fragments: DIFDPL (Diaphragmitic Induced Factor Dinucleotide Lipoprotein Lipoprotein), KPWALT (Keylanine White Amplified Transcription), LPFQRL (Lipoprotein Lipoprotein Quantitative Reducing Lipoprotein), FYFF (Factor Yeast Factor The Antarctic krill mixed peptide sample prepared by the invention has no cytotoxicity, and can promote cell proliferation, enhance cell NO secretion, improve cell phagocytic ability and effectively enhance immunity. The euphausia superba peptide prepared in the invention is easier to absorb, and has significant reduction and oxidation resistance. The polypeptide provided by the invention is a functional influence factor which has huge potential in the aspect of enhancing immunity, different bioactive peptides have different action mechanisms due to amino acid composition and structural characteristics, and theoretical support is provided for further developing food for enhancing the immunity function by utilizing the euphausia superba peptide. The development value of the euphausia superba is improved.

Embodiments disclose apparatus, methods and software for performing biological screening and analysis implemented using an instrument platform capable of detecting a wide variety of cell-based secretions, expressed proteins, and other cellular components. The platform may be configured for simultaneous multiplexed detection of a plurality biological components such that a large number of discrete samples may be individually sequestered and evaluated to detect or identify constituents from the samples in a highly parallelized and scalable manner.

The present invention discloses an anti-inflammatory peptide derived from quinoa and its use, belonging to the field of polypeptides. The technical problem to be solved by the present invention is how to inhibit the secretion of inflammatory mediators by immune cells. The polypeptide provided by the present invention (polypeptide SCAWLLAWSAPK) has an amino acid sequence as shown in SEQ ID NO: 1. The present invention also protects pharmaceutically acceptable salts of the polypeptide. The present invention also protects the use of the polypeptide or pharmaceutical salt in the preparation of products (such as drugs, reagents, or preparations) that inhibit inflammation. The inhibition of inflammation is manifested by inhibiting the secretion of inflammatory mediators by immune cells.

To provide a three-dimensional tissue structure that suppresses the inhibition of both functional structural formation and cell proliferation, while sustaining its thickness over an extended period.SOLUTION: A three-dimensional tissue structure contains cells and a matrix containing biocompatible molecules. The matrix is resistant to decomposition due to a substance secreted by the cells.SELECTED DRAWING: None

The application belongs to the technical field of medicine, and discloses a class of alkaloid compounds and the use of pharmaceutical compositions thereof in treating inflammation. In vitro cell experiments prove that the components have a significant inhibitory effect on the secretion of important inflammatory factors TNF-alpha and NO of lipopolysaccharide-induced RAW264.7 cells, and have a significant down-regulation effect on the phosphorylation level of key proteins of the NF-kappa B pathway in the inflammatory state. In vivo animal experiments prove that the components have a significant inhibitory effect on the tincture of croton oil-induced mouse ear swelling, and can be used for preventing and / or treating inflammation-related diseases.

The invention belongs to the technical field of exosomes, and discloses an exosome preparation method and application, the exosome preparation method comprises the following steps: S1, culturing target cells in an induction medium, and collecting the cells and a culture solution; s2, separating the culture solution to obtain supernate A and cell precipitate; s3, performing chromatographic purification and concentration on the supernate A to obtain the exosome; in the step S1, colchicine is also added in the process of culturing the target cells to synchronize the cell cycle; a polycaprolactone-gelatin composite microcarrier is suspended in the induction culture medium, and nicotinamide, an epidermal growth factor (EGF) and pyruvic acid are contained in the induction culture medium; the method further comprises a step S30 before the step S3, resuspending the cell precipitate obtained in the step S2 with a vesicle protection solution containing trehalose, sucrose and EDTA to obtain a supernatant B, and then combining the supernatant A with the supernatant B. According to the preparation method provided by the invention, the total secretion amount of target cells is large, the intracellular vesicle-exosome precursor can be collected, and the obtained exosome is high in concentration and low in impurity content.

A targeting vehicles comprises an extracellular vesicle with a dopamine transporter antibody on a transmembrane protein of the extracellular vesicle, the extracellular vesicle is secreted by a cell transfected with a vector gene, and at least a portion of the vector gene comprises SEQ ID No: 1. The targeting vehicles provided in the present invention can be loaded with drugs and cross the blood-brain barrier to achieve specific binding to dopamine neuron, and regulate the secretion of Parkinson's disease marker proteins and delay the course of Parkinson's disease.

The invention provides a tumor killing T extracellular vesicle release promoting method based on radio stimulation control, and belongs to the technical field of biological medicines.The yield of EVT secreted by T cells is remarkably increased by applying electrical stimulation to the T cells, an electrical stimulation system capable of being wirelessly controlled is established, efficient and controllable release of the T extracellular vesicles is achieved, and the tumor killing effect of the T extracellular vesicles is improved. The calcium ion dependency mechanism of the EVT is defined, and the function retention of the released EVT in tumor killing activity and tissue penetrability is verified.

This invention relates to a pharmaceutical composition for treating autoimmune diseases. The pharmaceutical composition for treating autoimmune diseases comprises an antibody and an inhibitor, wherein the antibody includes at least one of an OX40 antibody and an OX40L antibody, and the inhibitor includes at least one of a Janus kinase inhibitor and a RORγt inhibitor. In the above-mentioned pharmaceutical composition for treating autoimmune diseases, the Janus kinase inhibitor and / or the RORγt inhibitor can inhibit the secretion of IL17A by Treg cells induced by OX40 antibody and / or OX40L antibody, thereby reducing IL17A-promoted pathological inflammation and improving the efficacy of OX40 antibody and / or OX40L antibody in treating autoimmune diseases.

The present application relates to a composition capable of increasing the level of microRNA-26a in extracellular vesicles and the use of the composition, which can increase the level of microRNA-26a (miRNA-26a) in exosomes secreted from cells, and the exosomes can not only reduce the expression of biomarkers involved in muscle reduction, i.e. muscle ring finger protein 1 (MURF 1), human atrogin-1 (atrogin-1) and myostatin (myostatin), but also increase the expression of myogenic regulatory factor (myoD) involved in muscle generation. Therefore, the composition capable of increasing the level of microRNA-26a in extracellular vesicles can be used as an inhibitor of muscle loss and a promoter of muscle generation.

The present disclosure provides methods for mass tag labeling of cell secretomes and their soluble components, as well as mass-tagged soluble components of cell secretomes. In certain embodiments, the present disclosure provides methods for mass tagging of extracellular vesicles (EVs) and mass-tagged EVs. Methods of using mass-tagged soluble components of cell secretomes, such as mass-tagged EVs, mass-tagged viruses, or mass-tagged soluble proteins and peptides, are also provided. These can be combined with other labeling strategies, such as cell barcoding, that facilitate multiplex analysis and / or multidimensional analysis of the distribution, uptake, and effects of secretome components (such as EVs).

To provide a technique for selecting a target cell producing a target substance that specifically binds to a desired cell membrane protein more rapidly and efficiently. A substrate 1 having a plurality of microwells 2 is provided. A first cell 3 expressing a target cell membrane protein on its surface is allowed to adhere to each of the microwells 2. One or two second cells 5 as a candidate of a target cell are introduced into each microwell 2, and are allowed to coexist with the first cell 3 in the microwell 2, and target substance 6 secreted by the second cell 5 is brought into contact with the first cell 3. A microwell 2 including the first cell 3 to which the target substance 6 binds is identified. The second cell 5 as the target cell is recovered from the identified microwell 2. One example of the target substance 6 is an antibody. Visualization may be performed by adding a label substance 7.