265results about "Cell encapsulation" patented technology

Compositions for preventing or limiting surface fouling as well as evaporation and methods for their use in air-matrix digital microfluidics (DMF) apparatuses are described. A mobilizing wax material may be used to selectively encapsulate a reaction droplet in the air gap of the apparatus, which permits the at least partially encapsulated reaction droplet to be portable within the DMF apparatus. Additional aqueous droplets may be combined with the encapsulated droplet, by merging with an aqueous droplet having a coating of a secondary material (e.g., an oil or other hydrophobic material) that may allow combining of the droplets. The compositions may be additionally useful in non-DMF applications such as laboratory protocols for hybridization, ligation and amplification.

To provide non-naturally occurring three-dimensional (3D) brown adipose-derived stem cell aggregates, and methods of making and using the same.SOLUTION: The present application provides non-naturally occurring 3D brown adipose-derived stem cell (BADSC) aggregates, methods of making the 3D BADSC aggregates, and methods of using the 3D BADSC aggregates. Also provided herein is an encapsulation system comprising non-naturally occurring 3D brown adipose-derived stem cell aggregates. The 3D brown adipose-derived stem cell aggregate may comprise brown adipose-derived stem cells that express one or more brown adipocyte gene in the absence of differentiation medium.SELECTED DRAWING: None

The present application provides non-naturally occurring 3D brown adipose-derived stem cell (BADSC) aggregates, methods of making the 3D BADSC aggregates, and methods of using the 3D BADSC aggregates.

A composition comprising decellularized extracellular matrices, hydrogels comprising the same, and methods of making the same are disclosed.

Sub-millimeter scale three-dimensional (3D) structures are disclosed with customizable chemical properties and / or functionality. The 3D structures are referred to as drop-carrier particles. The drop-carrier particles allow the selective association of one solution (i.e., a dispersed phased) with an interior portion of each of the drop-carrier particles, while a second non-miscible solution (i.e., a continuous phase) associates with an exterior portion of each of the drop-carrier particles due to the specific chemical and / or physical properties of the interior and exterior regions of the drop-carrier particles. The combined drop-carrier particle with the dispersed phase contained therein is referred to as a particle-drop. The selective association results in compartmentalization of the dispersed phase solution into sub-microliter-sized volumes contained in the drop-carrier particles. The compartmentalized volumes can be used for single-molecule assays as well as single-cell, and other single-entity assays.

Provided herein are methods for coating a microparticle, cell, or therapeutic agent with a biomimetic zona pellucida (BZP). Also provided herein are BZP-coated cells prepared using the enclosed methods. The methods for using the BZP-coated microparticle to treat diabetes are also provided.

Provided herein are devices and methods for improved oxygenation of biological cells transplanted into a biological subject. The devices and methods may be used to promote vascularization to a transplantation site of the biological subject, and / or may be used to improve survival and functionality of cells contained within an encapsulation device.

There is provided a hydrogel, such as a functionalized hydrogel, for use in the expansion and / or differentiation of cells, that are encapsulated within the three-dimensional structure of the hydrogel. In some aspects of the invention, the hydrogel better mimics the thymus microenvironment than prior hydrogels for T cell expansion and differentiation and can be used in the expansion and differentiation of HSPCs, HSCs or PCs or combinations thereof in the production of T cells, T-cell committed precursors or progenitor cells and in methods for producing mature T cells. Methods of making the hydrogels, methods of culturing the cells in the hydrogel and uses of the hydrogels and cells produced are also described.

Sub-millimeter scale three-dimensional (3D) structures are disclosed with customizable chemical properties and / or functionality. The 3D structures are referred to as drop-carrier particles. The drop-carrier particles allow the selective association of one solution (i.e., a dispersed phased) with an interior portion of each of the drop-carrier particles, while a second non-miscible solution (i.e., a continuous phase) associates with an exterior portion of each of the drop-carrier particles due to the specific chemical and / or physical properties of the interior and exterior regions of the drop-carrier particles. The combined drop-carrier particle with the dispersed phase contained therein is referred to as a particle-drop. The selective association results in compartmentalization of the dispersed phase solution into sub-microliter-sized volumes contained in the drop-carrier particles. The compartmentalized volumes can be used for single-molecule assays as well as single-cell, and other single-entity assays.

The present disclosure provides methods of making tissue-derived epithelial organoids. In particular, the present disclosure provides a tissue-derived epithelial organoid embedded in a hydrogel suspended in a culture medium. The present disclosure further provides methods of using such organoids.

Embodiments of the present invention may provide protecting, preserving, and even repairing biological cells during in vitro processing. A uniform environment such as a protective layer with exogeneous lipids may be formed around biological cells which may interact with the plasma membranes of the cells.

A macroencapsulation device and associated manufacturing method are described in which a bonded membrane of the device may be attached to an associated frame in an arrangement that provides stress relief between the frame and a seal perimeter of the bonded membrane, and the seal perimeter may be disposed radially inward from an outer periphery of the membrane such that when the membrane is attached to a corresponding peripheral frame, the seal perimeter is spaced radially inward from the frame and an unbonded portion of one or more membranes is provided between the frame and the seal perimeter.

A therapeutic device includes a tubular body including a lumen extending therethrough, a wall of the tubular body including a first composite layer, a second composite layer; and a reservoir between the first and second composite layers, the reservoir being configured to receive and contain a biological moiety. In another embodiment, a therapeutic device includes a toroidal therapeutic device having a first opening therein and a hollow interior, the toroidal therapeutic device having a wall including a first permeable composite layer, a second permeable composite layer, and a reservoir between the first and second composite layers, the reservoir being configured to receive and contain a biological moiety.

Systems and methods consistent with the present invention generally relate to microorganospheres (MOSs), and methods and apparatuses for forming and using MOSs. More particularly, in some embodiments, systems and methods consistent with the invention relate to the methods and apparatuses for forming and using MOSs generated from hepatocytes. MOSs that are generated from hepatocytes are suitable for testing liver toxicity and drug induced liver injury effects of various agents.

Covalently modified alginate polymers, possessing enhanced biocompatibility and tailored physiochemical properties, as well as methods of making and use thereof, are disclosed herein. The covalently modified alginates are useful as a matrix for coating of any material where reduced fibrosis is desired, such as encapsulated cells for transplantation and medical devices implanted or used in the body.

The present invention relates to specific liver microtissues comprising hepatocytes of at least three different phenotypes derived from induced pluripotent stem cells encapsulated in a single three-dimensional closed microcompartment. The present invention also relates to methods for preparing such liver microtissues and their use in the treatment or prevention of liver failure.

The present disclosure provides thymic cells, thymic organoids, and animal models comprising such thymic cells and thymic organoids. It also provides methods for inducing differentiation of pluripotent stem cells into definitive endoderm (DE), anterior foregut endoderm (AFE), ventral pharyngeal endoderm (VPE), thymic epithelial progenitor (TEP) cells, thymic epithelial cells (TECs) and thymic organoids in vitro and in vivo.

The invention relates to a three-dimensional cellular microcompartment or an assembly of three-dimensional cellular microcompartments of ovoid, cylindrical, spheroidal or spherical shape or substantially ovoid, cylindrical, spheroidal or spherical shape, the smallest dimension of which is between 200 and 400 μm, comprising an external hydrogel layer defining an internal part, said internal part having a granzyme B content of less than 1 μg / ml of medium and comprising between 500 and 5000 lymphocytes forming a three-dimensionally grouped culture. The invention also relates to a method for producing such a microcompartment or microcompartment assembly.

A therapeutic device includes a pouch having opposed first and second ends and first and second composite layers extending between the opposed first and second ends. The first composite layer includes a first cell permeable layer extending between the opposed first and second ends and a first cell retentive layer extending between the opposed first and second ends. The pouch includes a reservoir formed between the first and second composite layers, contacting the first cell retentive layer, and at least one port in fluid communication with the reservoir. The therapeutic device further includes a removal element configured to operably engage the first end of the pouch, to cause the first end to be moveable towards the second end by eversion. In some embodiments, each of the first and second composite layers includes a plurality of wrinkles that are incrementally disengaged from a tissue to remove the therapeutic device atraumatically.

The present disclosure concerns genetic modification of fibroblasts or fibroblast-derived materials (including of the fibroblasts from which the fibroblast-derived materials are derived). In specific aspects, they are modified such that they may express one or more surface markers that allow targeting to a tissue and / or organ of interest. In some aspects, they are modified such that they may lose one or more surface markers that then allow targeting to a tissue and / or organ of interest. In certain aspects, one or more surface markers are modified that then allows targeting to a tissue and / or organ of interest.

Disclosed are methods and microfluidic devices for successfully co-encapsulating two or more cells in a high-throughput, high efficiency manner. Cells are organized into two or more ordered streams flowing through separate microchannels of the microfluidic device. Cells in ordered streams are sufficiently spaced such that at a junction of the microfluidic device, single droplets are generated that include exactly one cell from the first ordered stream of cells and at least one cell from the second ordered stream of cells. Single droplets including two or more cells are useful for performing assays (e.g., high throughput cell-cell interaction assays).

The present invention relates to a method for producing a stable hydrogel-cell composition comprising the steps of:a) providing a reaction mixture comprising at least one polymerizable substrate and at least one enzyme capable of polymerizing said at least one substrate, and cells, andb) incubating the mixture of step a) to form a stable hydrogel-cell composition.

Systems and methods for encapsulating cells in hydrogel tubes for cell growth within hydrogel tubes are described. In aspects, a system includes a plurality of peristaltic pumps and a plurality of pulse dampeners to provide oscillatory-controlled flow rates of each of a cell solution, a hydrogel solution, and a cross-linking solution to an extruder configured to form hollow hydrogel tubes having suspended biological cells in an interior

Methods and systems are provided for analyzing the ability of immune cells to kill target cells based on the effectiveness of mitochondria of the immune cells. The systems and methods further can be used for transplanting healthy mitochondria into immune cells to increase their effectiveness against target cells. The methods described herein can be used in treatment of cancer, mitochondrial diseases, and certain metabolic diseases.

The invention relates to a method to independently identify by fluorescence microscopy different cell types forming multicellular structures in co-culture thanks to a fluorescently-labelled extracellular matrix, and the individual identification of each multicellular structure of the co-culture with a spatial positioning of those structures in microcavities, so as to individually and independently measure multiple biological activities in each multicellular structure of each type of tissue of the co-culture.

A block for preparation of an observation section in accordance with the present disclosure includes: a cell aggregate that has a three-dimensional shape and that is embedded; and a marker that is continuous or intermittent and that extends in a predetermined direction.

New and improved hydrogels and methods associated therewith including their use for cell cultures and various other biomedical applications are disclosed. Because the instant invention does not rely on two dimensional surface area for cell growth but uses a three dimensional model, the hydrogels as disclosed herein have improved properties that allow for improved cell growth, easier and better harvesting and isolation of cells and small molecule products such as exosomes, as well as higher yields that can be achieved less expensively.

The invention discloses a hydrophobic polymer, a preparation method thereof and a single cell encapsulation method based on interaction of a subject and an object. The hydrophobic polymer disclosed by the invention can be embedded into a cell membrane to carry out hydrophobic modification on cells, and anchoring groups are modified on the surfaces of the cells, so that single-cell encapsulation of the cells is facilitated. The single cell encapsulation method based on interaction of the subject and the object comprises the following steps: 1) preparing hydrophobic polymer intercalation modified cells; and 2) carrying out layer-by-layer packaging on the hydrophobic polymer intercalation modified cells by using the cyclodextrin grafted hyaluronic acid and the adamantane grafted hyaluronic acid. The single cell encapsulation method based on host-guest interaction has the advantages of high encapsulation efficiency, high cell survival rate, good biocompatibility, simplicity in operation, mild reaction process, high reaction speed and the like, is suitable for large-scale industrial application, and cells obtained by encapsulation can be used for tissue repair in multiple aspects and are wide in application range.