185results about "Cell encapsulation" patented technology

To provide non-naturally occurring three-dimensional (3D) brown adipose-derived stem cell aggregates, and methods of making and using the same.SOLUTION: The present application provides non-naturally occurring 3D brown adipose-derived stem cell (BADSC) aggregates, methods of making the 3D BADSC aggregates, and methods of using the 3D BADSC aggregates. Also provided herein is an encapsulation system comprising non-naturally occurring 3D brown adipose-derived stem cell aggregates. The 3D brown adipose-derived stem cell aggregate may comprise brown adipose-derived stem cells that express one or more brown adipocyte gene in the absence of differentiation medium.SELECTED DRAWING: None

A composition comprising decellularized extracellular matrices, hydrogels comprising the same, and methods of making the same are disclosed.

Sub-millimeter scale three-dimensional (3D) structures are disclosed with customizable chemical properties and / or functionality. The 3D structures are referred to as drop-carrier particles. The drop-carrier particles allow the selective association of one solution (i.e., a dispersed phased) with an interior portion of each of the drop-carrier particles, while a second non-miscible solution (i.e., a continuous phase) associates with an exterior portion of each of the drop-carrier particles due to the specific chemical and / or physical properties of the interior and exterior regions of the drop-carrier particles. The combined drop-carrier particle with the dispersed phase contained therein is referred to as a particle-drop. The selective association results in compartmentalization of the dispersed phase solution into sub-microliter-sized volumes contained in the drop-carrier particles. The compartmentalized volumes can be used for single-molecule assays as well as single-cell, and other single-entity assays.

Provided herein are methods for coating a microparticle, cell, or therapeutic agent with a biomimetic zona pellucida (BZP). Also provided herein are BZP-coated cells prepared using the enclosed methods. The methods for using the BZP-coated microparticle to treat diabetes are also provided.

The present disclosure provides methods of making tissue-derived epithelial organoids. In particular, the present disclosure provides a tissue-derived epithelial organoid embedded in a hydrogel suspended in a culture medium. The present disclosure further provides methods of using such organoids.

Embodiments of the present invention may provide protecting, preserving, and even repairing biological cells during in vitro processing. A uniform environment such as a protective layer with exogeneous lipids may be formed around biological cells which may interact with the plasma membranes of the cells.

A macroencapsulation device and associated manufacturing method are described in which a bonded membrane of the device may be attached to an associated frame in an arrangement that provides stress relief between the frame and a seal perimeter of the bonded membrane, and the seal perimeter may be disposed radially inward from an outer periphery of the membrane such that when the membrane is attached to a corresponding peripheral frame, the seal perimeter is spaced radially inward from the frame and an unbonded portion of one or more membranes is provided between the frame and the seal perimeter.

A therapeutic device includes a tubular body including a lumen extending therethrough, a wall of the tubular body including a first composite layer, a second composite layer; and a reservoir between the first and second composite layers, the reservoir being configured to receive and contain a biological moiety. In another embodiment, a therapeutic device includes a toroidal therapeutic device having a first opening therein and a hollow interior, the toroidal therapeutic device having a wall including a first permeable composite layer, a second permeable composite layer, and a reservoir between the first and second composite layers, the reservoir being configured to receive and contain a biological moiety.

Systems and methods consistent with the present invention generally relate to microorganospheres (MOSs), and methods and apparatuses for forming and using MOSs. More particularly, in some embodiments, systems and methods consistent with the invention relate to the methods and apparatuses for forming and using MOSs generated from hepatocytes. MOSs that are generated from hepatocytes are suitable for testing liver toxicity and drug induced liver injury effects of various agents.

The present invention relates to specific liver microtissues comprising hepatocytes of at least three different phenotypes derived from induced pluripotent stem cells encapsulated in a single three-dimensional closed microcompartment. The present invention also relates to methods for preparing such liver microtissues and their use in the treatment or prevention of liver failure.

The present disclosure provides thymic cells, thymic organoids, and animal models comprising such thymic cells and thymic organoids. It also provides methods for inducing differentiation of pluripotent stem cells into definitive endoderm (DE), anterior foregut endoderm (AFE), ventral pharyngeal endoderm (VPE), thymic epithelial progenitor (TEP) cells, thymic epithelial cells (TECs) and thymic organoids in vitro and in vivo.

The invention relates to a three-dimensional cellular microcompartment or an assembly of three-dimensional cellular microcompartments of ovoid, cylindrical, spheroidal or spherical shape or substantially ovoid, cylindrical, spheroidal or spherical shape, the smallest dimension of which is between 200 and 400 μm, comprising an external hydrogel layer defining an internal part, said internal part having a granzyme B content of less than 1 μg / ml of medium and comprising between 500 and 5000 lymphocytes forming a three-dimensionally grouped culture. The invention also relates to a method for producing such a microcompartment or microcompartment assembly.

The present disclosure concerns genetic modification of fibroblasts or fibroblast-derived materials (including of the fibroblasts from which the fibroblast-derived materials are derived). In specific aspects, they are modified such that they may express one or more surface markers that allow targeting to a tissue and / or organ of interest. In some aspects, they are modified such that they may lose one or more surface markers that then allow targeting to a tissue and / or organ of interest. In certain aspects, one or more surface markers are modified that then allows targeting to a tissue and / or organ of interest.

Disclosed are methods and microfluidic devices for successfully co-encapsulating two or more cells in a high-throughput, high efficiency manner. Cells are organized into two or more ordered streams flowing through separate microchannels of the microfluidic device. Cells in ordered streams are sufficiently spaced such that at a junction of the microfluidic device, single droplets are generated that include exactly one cell from the first ordered stream of cells and at least one cell from the second ordered stream of cells. Single droplets including two or more cells are useful for performing assays (e.g., high throughput cell-cell interaction assays).

The present invention relates to a method for producing a stable hydrogel-cell composition comprising the steps of:a) providing a reaction mixture comprising at least one polymerizable substrate and at least one enzyme capable of polymerizing said at least one substrate, and cells, andb) incubating the mixture of step a) to form a stable hydrogel-cell composition.

Systems and methods for encapsulating cells in hydrogel tubes for cell growth within hydrogel tubes are described. In aspects, a system includes a plurality of peristaltic pumps and a plurality of pulse dampeners to provide oscillatory-controlled flow rates of each of a cell solution, a hydrogel solution, and a cross-linking solution to an extruder configured to form hollow hydrogel tubes having suspended biological cells in an interior

Methods and systems are provided for analyzing the ability of immune cells to kill target cells based on the effectiveness of mitochondria of the immune cells. The systems and methods further can be used for transplanting healthy mitochondria into immune cells to increase their effectiveness against target cells. The methods described herein can be used in treatment of cancer, mitochondrial diseases, and certain metabolic diseases.

The invention relates to a method to independently identify by fluorescence microscopy different cell types forming multicellular structures in co-culture thanks to a fluorescently-labelled extracellular matrix, and the individual identification of each multicellular structure of the co-culture with a spatial positioning of those structures in microcavities, so as to individually and independently measure multiple biological activities in each multicellular structure of each type of tissue of the co-culture.

The invention discloses a hydrophobic polymer, a preparation method thereof and a single cell encapsulation method based on interaction of a subject and an object. The hydrophobic polymer disclosed by the invention can be embedded into a cell membrane to carry out hydrophobic modification on cells, and anchoring groups are modified on the surfaces of the cells, so that single-cell encapsulation of the cells is facilitated. The single cell encapsulation method based on interaction of the subject and the object comprises the following steps: 1) preparing hydrophobic polymer intercalation modified cells; and 2) carrying out layer-by-layer packaging on the hydrophobic polymer intercalation modified cells by using the cyclodextrin grafted hyaluronic acid and the adamantane grafted hyaluronic acid. The single cell encapsulation method based on host-guest interaction has the advantages of high encapsulation efficiency, high cell survival rate, good biocompatibility, simplicity in operation, mild reaction process, high reaction speed and the like, is suitable for large-scale industrial application, and cells obtained by encapsulation can be used for tissue repair in multiple aspects and are wide in application range.

Disclosed are devices and methods for transplanting cells, such as pancreatic endoderm cells, into a host. The devices include a non-woven fabric external to a cell-excluding membrane, and the non-woven fabric and / or cell-excluding membrane can be perforated. Treatment of the host with immunosuppressive reagents, required to inhibit allograft rejection due to perforations in the cell delivery device, does not compromise maturation or function of transplanted pancreatic endoderm cells.

Provided herein are modified gelatins, cross-linked modified gelatins and hydrogels, and processes for making the same. Also provided herein is the use of such materials as matrices for cell growth.

The invention relates to a neural tissue unit for use in implantation into the nervous system of a human or non-human mammal, wherein said neural tissue unit contains differentiated post-mitotic neuronal cells in an extracellular matrix, said unit being obtained from a cellular microcompartment comprising a hydrogel capsule surrounding the neural tissue unit, and said hydrogel capsule being at least partially removed before use of the neural tissue unit. The invention also relates to a process for preparing such a neural tissue unit.

The present invention relates to a method for culturing skin-derived stem cells and uses thereof, and to a method for culturing skin-derived stem cells; skin-derived stem cells produced by the method; a pharmaceutical composition for skin regeneration or wound treatment comprising the skin-derived stem cells produced by the method, a culture thereof, or cells differentiated from the skin-derived stem cells as an active ingredient; a quasi-drug composition; and a cosmetic composition. According to the culture method of the present invention, stem cells can be effectively isolated and obtained from skin tissue. The obtained skin-derived stem cells have excellent pluripotency and self-renewal ability, and have excellent skin regeneration and wound treatment effects, and can therefore be usefully used for skin regeneration and wound treatment or improvement applications.

The present invention relates to a method for culturing hair follicle-derived stem cells and uses thereof, and to a method for culturing hair follicle-derived stem cells; hair follicle-derived stem cells produced by the method; a pharmaceutical composition for treating hair loss or promoting hair growth, comprising the hair follicle-derived stem cells produced by the method, a culture thereof, or cells differentiated from the hair follicle-derived stem cells as active ingredients; a quasi-drug composition; and a cosmetic composition. According to the culture method of the present invention, stem cells can be effectively isolated and obtained from hair follicle tissue. The obtained hair follicle-derived stem cells have excellent pluripotency and self-renewal ability, and are effective in treating hair loss and promoting hair growth, and therefore can be usefully used for improving, preventing or treating hair loss.

The present disclosure provides, inter alia, a bilayer semipermeable fibrosis-resistant hydrogel polymer enclosure optionally containing cells such as mammalian cells, the enclosure being suitable for administration to a subject in need thereof, and further, the enclosure being expandable in accordance with a large number of delivered cells. The present disclosure further provides methods of making and using the enclosure, for example, in a treatment method, for example, in the treatment of disorders of the liver or other cells and organs treatable with secreting cells and / or catalytic cells.

The invention relates to a neural tissue unit for use in implantation into the nervous system of a human or non-human mammal, wherein said neural tissue unit contains differentiated post-mitotic neuronal cells in an extracellular matrix, said unit being obtained from a cellular microcompartment comprising a hydrogel capsule surrounding the neural tissue unit, and said hydrogel capsule being at least partially removed before use of the neural tissue unit. The invention also relates to a process for preparing such a neural tissue unit.

An implantable therapeutic delivery device comprising a porous pouch and a cell encapsulation device configured to be housed in the porous pouch. The porous pouch can comprise a bioabsorbable material and / or a vascularization promoter, and the porous pouch can be packaged separately from the cell encapsulation device.

This application provides a cell encapsulation stromal material and its manufacturing method and application. The material is prepared by cutting, low-temperature microparticulation grinding, enzymatic decomposition, cell component removal, virus inactivation, freeze-drying and radiation sterilization using cartilage from mammals such as pigs, cows, sheep, horses and deer. The method is not for diagnostic purposes, and the stromal material is used for 3D bioprinting alone or with encapsulated cells. The main components of the material are type II collagen, chondroitin sulfate and hyaluronic acid, and have the advantages of no crosslinking toxicity, low immunogenicity, good biocompatibility and can be used for human implantation. In terms of structure, it has a good surface topology structure, provides a tissue microenvironment similar to that in the body for in vitro cell culture, and can be used as a microcarrier for cell culture, as well as promote the formation of cell mass, and can encapsulate cells in the gel-state stromal during the process of cell storage and transportation, protect cells from the effects of adverse environments and improve the viability of cells.

The invention discloses a method for large-scale cryopreservation of animal cells. The method comprises the following steps: carrying out first-stage amplification on to-be-preserved cells under the support of matrigel; performing second-stage amplification on the to-be-preserved cells subjected to the first-stage amplification under the support of a microcarrier; mixing the to-be-preserved cells with a serum-free cryopreservation solution to prepare a micro-capsule suspension; and putting the microcapsule suspension into a cryopreservation container to carry out cryopreservation. According to the invention, through amplification of two stages, the to-be-preserved cells have reached a high density of 109 orders of magnitude before cryopreservation, a re-amplification link in a cell recovery stage can be greatly shortened, and the to-be-preserved cells are subjected to microencapsulation through the micro-fluidic chip and then cryopreserved, so that physical protection is given to the to-be-preserved cells; the damage of ice crystals in the cryopreservation process is reduced, the survival rate after resuscitation is improved, and the microencapsulated cells can also be directly subcultured, so that the resuscitation operation is simplified; according to the cell cryopreservation device, the cryopreservation container with a large volume is used, so that the cost of cell cryopreservation is reduced.

The present invention provides a method of high-density cell culture comprising at least the following steps: S1: obtain hydrogel microcapsules through membrane emulsification; S2: encapsulate a selected number of cells per each obtained hydrogel microcapsule, and S3: culture the encapsulated cells in a cell culture medium. The invention further refers to Human Pluripotent Stem Cells (hPSCs) obtained through the above-described method, in particular for use in medicine, more in particular for use in a method of in vitro generation of mature megakaryocytes.