37 results about "Swelling ratio" patented technology

The invention discloses a bi-crosslinking gelatin-based hydrogel wound dressing as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The preparation method disclosed by the invention comprises the following steps: mixing a gelatin solution and an oxidized konjac glucomannan solution, heating and stirring to obtain a hydrogel precursor solution; and mixing the hydrogel precursor solution with a borax solution to obtain the bi-crosslinked gelatin-based hydrogel wound dressing. According to the method, oxidized konjac glucomannan and gelatin are subjected to Schiff base and Schiff base crosslinking, borax is further added to introduce a dynamic boric acid ester bond effect to enhance a three-dimensional network of the hydrogel, and the dynamic reversible Schiff base effect and the boric acid ester bond effect are enhanced, so that the pore diameter and the swelling ratio of the hydrogel are increased, and the gel strength of the hydrogel is enhanced; due to the good self-healing injectable performance, biodegradability and mechanical property of the hydrogel, the hydrogel can be used as a wound dressing for irregular deep skin wounds, and the technical problem that existing hydrogel is insufficient in treatment of irregular wounds is solved.

According to the injectable double-layer drug sustained-release hydrogel and the preparation method and application thereof, hydrogel swelling and degradation curve and mechanical property detection shows that extrusion secondary injury to optic nerves can be avoided due to the swelling ratio ratio of 14% of inner-layer hydrogel to 10% of outer-layer hydrogel; the hydrogel precursor solution prepared by the preparation method disclosed by the invention has proper mechanical strength and adhesive force so as to cope with an in-vivo environment, has good injectability, can control the gelling time within 2 minutes, perfectly conforms to a short time window of optic nerve decompression surgery, and has extremely high clinical practical value. The problems that the serious operation risk of cerebrospinal fluid leakage during optic nerve local administration and a traditional local administration carrier lacks in-situ stability and is prone to displacement are solved, the damaged microenvironment can be cooperatively regulated and controlled, and long-acting slow release of nerve regeneration is promoted; multiple functions such as operation safety, medicine long-acting slow release, biocompatibility and operation convenience are successfully integrated, and an unprecedented effective strategy is provided for solving the clinical problem of optic nerve injury.

The invention belongs to the field of material science and clinical medicine, and particularly relates to a succinic acid polymer and a preparation method and derivative thereof, adhesive hydrogel and a preparation method and application thereof. According to the specific technical scheme, the succinic acid polymer is provided, and the succinic acid acrylate polymer is prepared by modifying the succinic acid polymer through acryloyl chloride; further uniformly mixing a succinic acid acrylate polymer, acrylic acid, dimethylaminoethyl methacrylate and acrylic acid N-hydroxysuccinimide ester with a photoinitiator phenyl-2, 4, 6-trimethylbenzoyl lithium phosphite, and reacting under the irradiation of ultraviolet light to obtain the photoinitiator. The three-dimensional network structure hydrogel with strong wet adhesion, low swelling rate and high toughness is prepared through a photopolymerization reaction. The hydrogel has good biocompatibility, an in-vivo rabbit model proves that an orificium fistulae can be completely sealed through single use or combined suture, a safe and efficient novel biological material is provided for anastomotic fistula treatment, and the hydrogel has important clinical transformation potential.

The invention discloses low-swelling and asymmetric-adhesion Janus hydrogel as well as a preparation method and application of the low-swelling and asymmetric-adhesion Janus hydrogel. The raw materials of the hydrogel comprise a surfactant, a hydrophilic monomer, a hydrophobic monomer, PABA, natural polyphenol and a photoinitiator. The preparation method comprises the following steps: dissolving a surfactant and a hydrophilic monomer in water, then adding a hydrophobic monomer, PABA and natural polyphenol, uniformly mixing at a certain stirring speed, then adding a photoinitiator to obtain a hydrogel precursor emulsion, and cross-linking under ultraviolet light to obtain the hydrogel. The hydrogel provided by the invention forms a nano-scale micro-phase separation structure through electrostatic interaction of internal carboxyl and quaternary ammonium salt groups, so that the swelling behavior of the hydrogel can be effectively inhibited, the hydrogel has a relatively low swelling rate, and internal mechanical properties are enhanced; meanwhile, the hydrogel has remarkable asymmetric adhesion performance, one side has strong adhesion, the other side has weak adhesion, and postoperative adhesion is expected to be avoided while wound tissues are repaired.

The invention discloses a temperature-sensitive slow-release medical auxiliary material and a preparation method of the temperature-sensitive slow-release medical auxiliary material, and belongs to the technical field of medical auxiliary materials. Comprising the following components: 1-2% w / v of acrylamide hydroxyethyl chitosan, 5-15% w / v of polyethylene glycol diacrylate, 1-6% w / v of N-methyl acrylate caprolactam, 0.05-2% w / v of an initiator, 0.01-0.5% w / v of a cross-linking agent and the balance of a PBS (Phosphate Buffer Solution), the acrylamide hydroxyethyl chitosan is modified by chitosan hydroxyl group and ethyl group; the N-methyl acrylate caprolactam monomer provided by the invention effectively improves the temperature sensitivity of the temperature-sensitive polymer auxiliary material; the acrylamide hydroxyethyl chitosan can reduce the swelling ratio and slow down the drug release; the polyethylene glycol diacrylate can be a crosslinking monomer of the temperature-sensitive polymer auxiliary material, meanwhile, an ether bond is introduced to improve the biocompatibility of the temperature-sensitive polymer auxiliary material, and the temperature-sensitive slow-release medical auxiliary material which is low in drug slow-release rate and responds to the temperature of a human body is prepared by combining the polyethylene glycol diacrylate, the ether bond and the temperature-sensitive polymer auxiliary material.

The invention provides temperature-controlled hydrogel and a preparation method thereof, and belongs to the technical field of compositions of high-molecular compounds. The preparation method of the temperature control hydrogel comprises the following steps: preparing a polyglutamic acid-metacrylic acid ester copolymer, preparing composite chitosan, modifying fullerene with chitosan, and mixing. According to the temperature control hydrogel prepared by the invention, the swelling ratio of the temperature control hydrogel is 19.97-21.72 g / g, the swelling ratio of the hydrogel is high, the hydrophilicity of a hydrogel network is strong, the fullerene dispersity is good, and the biocompatibility is enhanced; the compression strength of the temperature control hydrogel ranges from 149.7 kPa to 152.3 kPa, the compression modulus ranges from 25.9 kPa to 28.6 kPa, the fracture deformation ranges from 70.5% to 73.1%, the mechanical performance of the temperature control hydrogel is excellent, and the critical solution temperature of the prepared hydrogel ranges from 31.12 DEG C to 31.35 DEG C.

This invention belongs to the field of biomaterials technology and discloses a two-component in-situ crosslinking agent, a biodegradable two-component hydrogel, and their applications. The two-component in-situ crosslinking agent disclosed in this invention has injectability and rapid gelation properties. The hydrogel formed after crosslinking has a low self-swelling rate, good elasticity, and is not easily crushed. It also has a suitable metal ion slow-release cycle and degradation cycle for preventing tissue adhesion or tissue repair. It is especially suitable for high-risk areas of postoperative adhesion such as the uterine cavity, abdominal cavity, and joints, and can also promote tissue regeneration.

The invention discloses a low-swelling-rate hydrogel material based on natural polysaccharide and a preparation method and application thereof, and belongs to the technical field of implantable medical devices. The low-swelling-rate hydrogel material is of a double-crosslinking network structure composed of a first crosslinking network and a second crosslinking network, the first crosslinking network is a covalent crosslinking network formed by zwitterionic monomers through free radical polymerization, and the second crosslinking network is a covalent crosslinking network formed by zwitterionic monomers through free radical polymerization. The second cross-linked network is a physical cross-linked network formed by coordination of alginate and metal ions. The low-swelling-rate hydrogel material provided by the invention has the characteristic of low swelling rate in normal saline, and can still reach compression stress and compression modulus equivalent to those of commercially available breast prostheses after balanced swelling in the normal saline.

The invention belongs to the technical field of anti-adhesion materials in biomedical engineering, and relates to an anti-adhesion membrane with coarse structure fibers and a preparation method of the anti-adhesion membrane. The method comprises the following steps: soaking an electrostatic spinning fiber membrane containing a high polymer material and a hydrophilic anti-adhesion component in a treating fluid containing the high polymer material and consisting of a poor solvent and a good solvent for a specific time, rinsing and drying to obtain the anti-adhesion membrane, the swelling ratio of the high polymer material in the treating fluid at 25 DEG C for 10 minutes is 1-10% due to the proportion of the inferior solvent to the benign solvent in the treating fluid. A stable integrated nano shish-kebab structure is formed on the surface of the prepared anti-adhesion membrane fiber, exogenous drugs are not needed, spreading of fibroblasts is limited by means of a physical topological structure, conversion of the fibroblasts to myofibroblasts is blocked, the anti-adhesion effect is good, and biocompatibility is good.

The application discloses an injectable double-layer drug sustained-release hydrogel and a preparation method and application thereof, and relates to the field of drug sustained-release hydrogels. The swelling and degradation curve and mechanical property detection of the hydrogel show that, when the swelling rate ratio of the inner layer hydrogel to the outer layer hydrogel is 14% to 10%, the secondary damage of the optic nerve caused by extrusion can be avoided; meanwhile, the hydrogel has suitable mechanical strength and adhesion to cope with the in-vivo environment. The hydrogel precursor solution has good injectability, and the gelation time can be controlled within 2 minutes, which perfectly matches the short time window of the optic nerve decompression surgery, and has high clinical practical value. The application solves the major surgical risk of cerebrospinal fluid leakage when the optic nerve is locally administered, and solves the problems of the traditional local drug carrier, such as lack of in-situ stability and easy displacement. The application can synergistically regulate the injury microenvironment and promote the long-acting sustained-release of nerve regeneration, successfully integrates multiple functions such as surgical safety, drug long-acting sustained-release, biocompatibility and operation convenience, and provides an unprecedented effective strategy for solving the clinical problem of the optic nerve injury.

The invention relates to polymer hydrogel with high elastic modulus, which is formed by crosslinking polysaccharide and multivalent metal ions, the weight ratio of the multivalent metal ions to the polysaccharide is (0.01-1)%: 1, and the elastic modulus of the hydrogel is not lower than 1000kPa, preferably not lower than 3000kpa. The space structure of polysaccharide is semi-stretched under the action of an alcohol-water solution to complete intramolecular crosslinking, then polyvalent metal ions are added to achieve intermolecular crosslinking of polysaccharide and metal ions, internal and external consideration is achieved, the prepared hydrogel has the high swelling rate under the acid and weak alkaline conditions, the swelling time is short (the swelling equilibrium is achieved within 30 min), and the hydrogel has the good application prospect. And the swelling duration is long (the swelling rate is not less than 80g / g and the time is not less than 3h).

Provided is an adhesion prevention film that sufficiently swells upon contact with moisture and is less prone to breaking. This adhesion prevention film contains a bioabsorbable polymer compound. The adhesion prevention film has a thickness of 10-500 µm, and a swelling ratio of 200-500 vol% after 15-minute immersion in phosphate buffer at pH 6.0. In the case where 1H-NMR measurement of the film is performed, using a sample solution prepared by adding the film and 1.2 mg of pyrazine to 1.1 mL of deuterated trifluoroacetic acid, with the resonance peak of pyrazine as a standard substance referenced to 9.45 ppm, when the integral value of the maximum resonance peak measured in the range of 9.4-9.5 ppm is defined as A and the integral value of the maximum resonance peak measured in the range of 5.0-5.2 ppm is defined as B, B / A is 0.10 or less.

The invention discloses a multi-effect modified biodegradable composite material and a preparation method thereof. Relates to a biodegradable material and a preparation method thereof. The modified PLA / PBS composite material comprises the following raw materials: 30-90 parts of polylactic acid (PLA); 10 to 70 parts of poly (butylene succinate) (PBS); 1-10 parts of an environment-friendly auxiliary agent; and 0.1 to 0.5 part of an antioxidant. And drying the raw materials, and carrying out twin-screw extrusion melt blending. Infrared spectrum characteristic analysis, a nuclear magnetic resonance hydrogen spectrum test and a swelling rate test show that the compatibility between the materials is improved by introducing the auxiliary agent, the notch impact strength and the elongation at break of the material are respectively improved to 5178.1 J / m and 247.93% on the basis of keeping high rigidity, and are respectively improved by 184% and 103% compared with those of an unmodified system, meanwhile, the light transmittance is kept unchanged, the haze is reduced by 28.3%, and the material has a good application prospect. The excellent hydrophobicity is realized. The composite material can be completely biodegraded, is suitable for the fields of environment-friendly packaging, disposable products and the like, and has the characteristics of high performance and eco-friendliness.

The application provides a high-efficiency hemostatic dressing, a preparation method and application thereof. The hemostatic dressing is a crosslinked product containing hydroxypropyl chitosan and low molecular weight eucommia ulmoides gum. The hemostatic dressing has a high swelling rate, can better absorb water-soluble exudates, and is beneficial to rapid hemostasis. The hemostatic dressing has better mechanical strength, which indicates that the hemostatic dressing has excellent compression resistance and high-load impact resistance, so that appropriate eucommia ulmoides gum-based dressing can be selected according to specific application scenes and wound characteristics, so as to improve the treatment effect.

This invention provides a photothermal antibacterial hydrogel dressing for NIR-II, its preparation method, and its application. The preparation method involves mixing a 10%–15% PVA aqueous solution with an equal volume of other solutions, then mixing this mixture with CMCS and sodium tetraborate solution, stirring, and centrifuging to obtain the PBCM hydrogel. Each 1 ml of PVA solution corresponds to 40 mg of CMCS. The mass ratio of CMCS to sodium tetraborate solute is 10:1, and the solution concentration is greater than 0 μg / ml and less than or equal to 300 μg / ml. The hydrogel dressing prepared using this method exhibits mechanical strength, high swelling ratio (up to 798.45%), self-healing properties, and biocompatibility. Under low-power near-infrared II laser irradiation, it demonstrates a high antibacterial rate.

The invention discloses a carboxymethyl cellulose composite hydrogel dressing for promoting wound healing and a preparation method thereof, and belongs to the technical field of biological materials. According to the hydrogel dressing, oxidized carboxymethyl cellulose (OCMC), quaternized chitosan and polydopamine nanoparticles are cross-linked through Schiff base bonds and amido bonds to form a dual-network structure. The preparation method comprises the following steps: dissolving the OCMC in water to form a solution, adding the polydopamine nanoparticles to enable the final concentration to reach 7.5-30mg / mL, mixing with a quaternized chitosan solution, and standing for cross-linking. The hydrogel disclosed by the invention has the advantages of short gelation time (less than or equal to 30 seconds), strong tissue adhesion (the overlapping shear strength can reach 40kPa), excellent self-healing and injectable performance, high swelling ratio (greater than or equal to 400% within 1 hour) and broad-spectrum antibacterial activity, can effectively absorb percolate, reduce inflammation, accelerate collagen deposition and remarkably promote healing of infected wounds, and is particularly suitable for preparing irregular wound dressing.

The invention provides a tissue phase homogenizing agent and a preparation method and application thereof, and belongs to the technical field of biological tissue engineering, the homogenizing agent is composed of hydrophilic matrixes (glucose, polyglycerol, poloxamer F127 and polyvinyl alcohol), functional additives (mannose, N-acetylcysteine, sodium hyaluronate and polyethylene glycol) and ultrapure water, and the pH value is 7.0 + / -0.2. Hydrophilic groups and water molecules form a hydrogen bond network, a continuous hydration layer is constructed on a phase interface, phase domain fusion, refractive index homogenization and molecular ordering are achieved, the problem of biological tissue phase homogenization is solved, tissue light transmittance can be improved, the swelling ratio can be stabilized, and the hydrogel has cell proliferation promoting activity and is suitable for tissue transplantation operation and bioengineering tissue preservation.

The application discloses a kind of fast swelling, enzyme catalytic degradation hydrogel and its preparation method and application, in the present application based on by sucrose molecule connection multi-arm hydrophilic polymer crosslinked hydrogel, and the swelling rate is fast, and the swelling rate is high, and in the environment of sucrose enzyme degradation rate is fast, can be widely used for preparing in enzyme response biodegradable hydrogel carrier and promote weight loss product.

The invention discloses a method for testing the gel content of a cable insulation material, and relates to the field of material detection. The method comprises the following steps: crushing a sample to be detected, weighing, and recording as W1; after a solvent is added, heating treatment is carried out, and the heating temperature is 90-115 DEG C; the solvent comprises xylene and tetrachloroethane; taking out, drying, cooling, weighing, and recording as W2; and then calculating the gel content of the sample. According to the method, the solvent containing xylene and tetrachloroethane is adopted to extract the to-be-detected sample under a high-temperature condition, the non-uncrosslinked part of the cable insulation material is completely dissolved by using the xylene non-polar solvent, and the polar chain segment is effectively extracted by using the tetrachloroethane polar solvent, so that the affinity and the swelling rate of the solvent to the cable insulation material are improved; aiming at a cable insulation material grafted or copolymerized with polar groups, the accuracy of testing the gel content can be effectively improved.

The invention provides a preparation method of collagen-based microspheres and application of the collagen-based microspheres in preparation of a subcutaneous tissue filling material, and relates to the technical field of medical treatment and medical cosmetology. According to verification of a large number of experiments, the collagen-based microspheres are prepared through a two-step cross-linking method and adjustment of polarity of a solvent used in the second-step chemical cross-linking process; the mass swelling ratio and the effective crosslinking density of the collagen-based microspheres can be regulated and controlled; different from a traditional filling material which only depends on physical support, the collagen-based microspheres with specific mass swelling ratio and crosslinking density have good injectability and mechanical support performance and can realize subcutaneous cell recruitment and promote cell proliferation and extracellular matrix secretion, so that a long-acting and stable subcutaneous tissue filling effect is realized. In addition, the collagen-based microspheres have good biocompatibility and degradability, and conform to the idea of cell-free tissue engineering.

The application belongs to the technical field of hydrogel preparation, and relates to a high-strength low-friction swelling-resistant self-adhesive hydrogel material and a preparation method. The preparation method of the hydrogel comprises four steps of preparation of a precursor hydrogel containing a drag-reducing layer and a tough layer, preparation of a mineralization layer, pre-swelling, and preparation of an adhesive layer. The obtained hydrogel has a four-layer structure of the drag-reducing layer, the tough layer, the mineralization layer, and the adhesive layer. The preparation method is simple, and ideal effects are obtained through simple soaking and smearing. The obtained four-layer hydrogel has high strength, low friction, swelling resistance, self-adhesion, and excellent bone induction potential. The water content of the hydrogel is about 70%, the swelling rate is close to 0, the compressive strength can reach 78 MPa, the friction coefficient is as low as 0.024, the adhesion strength to the bone and the skin is as high as 123 kPa and 79 kPa, respectively. The performance of the hydrogel obtained by the application is better than the comprehensive level of the existing cartilage replacement hydrogel material, and has important significance for widening the application of the hydrogel in the field of artificial cartilage.

This application discloses an injectable wet-adhesive hydrogel, its preparation method, and its application, belonging to the field of biomedical materials technology. The hydrogel of this application is formed by in-situ covalent cross-linking of liquid component A and liquid component B. Liquid component A contains an aqueous solution of a four-arm polyethylene glycol succinimide ester compound; liquid component B contains an aqueous solution of serum albumin and an amino-containing biomedical polymer additive. Depending on the amino-containing biomedical polymer additive, the hydrogel has two composition schemes: one is a quaternary structure of a four-arm polyethylene glycol succinimide ester compound, serum albumin, polylysine, and sodium alginate; the other is a ternary structure of a four-arm polyethylene glycol succinimide ester compound, serum albumin, and a four-arm polyethylene glycol amine. This hydrogel possesses good injectability and low swelling rate, and can construct a multi-point covalent cross-linking mechanism combining chemical cross-linking and reversible physical cross-linking, significantly improving the wet adhesion performance and mechanical stability of the hydrogel, effectively achieving a long-term stable sealing effect during dynamic tissue activity in vivo.

The application discloses a preparation method of a double-network hydrogel coating and relates to the technical field of biomedical materials.The preparation method comprises the following steps: after the surface of a silica gel base material is modified, the silica gel base material is first immersed in a SBMA prepolymer liquid for a certain period of time, and then is heated in a water bath at 60-65 DEG C for a certain period of time; then the silica gel base material is taken out and immersed in a DST prepolymer liquid for a certain period of time; finally, the soaked silica gel base material is irradiated under ultraviolet light for a certain period of time to obtain the double-network hydrogel coating.The double-network hydrogel coating prepared by the method has a relatively stable swelling rate, and when the double-network hydrogel coating is applied as a coating on the inner surface of a silica gel suction disc, the double-network hydrogel coating can slightly swell to fill the tiny texture gullies existing in internal organs or skin, increase the air-tightness of adsorption, and effectively improve the adsorption effect of the silica gel suction disc on the internal organs or skin.

The invention provides a composite polyvinyl alcohol hydrogel with an antibacterial function and a preparation method thereof, and particularly relates to a hydrogel with an antibacterial function prepared by doping refined pyroligneous liquor and polyvinyl alcohol with different concentrations and adopting a freeze-thaw method, and the physical and chemical properties of the hydrogel are explored. The RWV / PVA composite hydrogel has the advantages that the antibacterial property of the RWV / PVA composite hydrogel is verified by the aid of staphylococcus aureus and escherichia coli, and the biological safety of the RWV / PVA composite hydrogel is investigated by the aid of cytotoxicity experiments of the hydrogel.

The invention provides a preparation method of gelatin-based microspheres and an application of the gelatin-based microspheres in preparation of a subcutaneous tissue filling material, and relates to the technical field of medical treatment and medical cosmetology, the inventor finds through a large number of experimental exploration verifications that the physical properties of the gelatin-based microspheres can be regulated and controlled by adjusting the polarity strength of a solvent used in a chemical crosslinking process; different from a traditional filling material only depending on physical support, gelatin-based microspheres with specific swelling ratio and crosslinking density can promote cell recruitment, proliferation and extracellular matrix secretion subcutaneously, so that long-acting and stable subcutaneous tissue filling is realized, the treatment effect can be optimized by adjusting components, and the cell-free tissue engineering concept is met. In conclusion, the gelatin-based microspheres with the specific swelling ratio, crosslinking density and components have the potential obviously superior to that of existing products in the fields of aging resistance, wound repair, postoperative tissue filling and the like, and the gelatin-based microspheres have the basis of becoming a new generation of high-performance subcutaneous tissue filling materials.

The present invention addresses the problem of providing a concentration jig with little variation in concentration ratio when a specimen liquid is concentrated. A concentration jig according to the present invention is used for concentration processing in inspection for detecting a polymer contained in a liquid sample, the concentration jig including: a water-absorbing polymer for absorbing at least part of the moisture of the liquid sample; and a container which accommodates the water-absorbing polymer, into which the liquid sample is taken, and at least a portion of which is flexible, wherein the water-absorbing polymer can be pressed through the inner wall of the container, and the swelling ratio of the water-absorbing polymer is between 0.2 g / g and 800 g / g inclusive.

The invention relates to the preparation and application of a hydrogel humidity sensor based on an optical detection method, which belongs to the field of new sensors. Acrylamide monomers are doped into an acrylic ester hydrogel in a certain proportion, and copolymerization is achieved by ultraviolet light-induced polymerization, which can increase the swelling rate of the hydrogel. Based on nanoimprint technology, a high-quality hydrogel grating was prepared by a "two-step transfer method". The hydrogel grating can sense humidity using an optical detection method. The present invention develops a fast, simple and controllable hydrogel grating preparation technology with low cost; the prepared hydrogel grating humidity sensor has high response sensitivity, good linearity, adjustable response range and good repeatability, and has good practicality and development prospects in biosensing, environmental monitoring and other aspects.

This invention belongs to the field of biomaterials and discloses a protein-based hydrogel in-situ crosslinking agent. The in-situ crosslinking agent is characterized by being composed of a first precursor solution and a second precursor solution. The first precursor solution is prepared by dissolving thiolated polyethylene glycol (PEGSH) and protein in a buffer solution and adjusting the pH to 7.2 with NaOH. The second precursor solution is prepared by dissolving CuSO4 in deionized water. This protein-based hydrogel in-situ crosslinking agent possesses injectability, rapid gelation properties, and the hydrogel formed after crosslinking exhibits strong self-healing ability, low swelling ratio, good elasticity, and is not easily crushed. Furthermore, it can rapidly disintegrate under the action of a degelating agent.

The invention relates to the technical field of hydrogel, in particular to premixed mediated cross-linked two-component polyethylene glycol hydrogel and a preparation method thereof. Comprising a first reagent and a second reagent, the first reagent comprises a double-component solution, and the second reagent comprises a single-component solution; the first reagent and the second reagent contain polyethylene glycol derivatives, and the mass concentration of the polyethylene glycol derivatives in the first reagent is 1-20%. The curing speed can be effectively increased by premixing the mediated and cured gel, compared with the gel which is not premixed, the gel has longer degradation time and better mechanical property, and a system which cannot be normally cured into gel can be converted into curable gel. The hydrogel provided by the invention has the technical effects of low solid content, low swelling rate and mechanical enhancement.

The invention belongs to the technical field of biological materials. The preparation steps of the existing composite injectable gel are tedious, and the prepared composite injectable gel has the problems of poor injectability, low swelling rate, low mechanical strength, low drug loading rate, high cytotoxicity and the like. On the basis, the invention provides a method for preparing the composite injectable gel, and the method comprises the following steps: 1) dissolving hyaluronic acid and sodium chloride in an aqueous solution of NaOH or KOH; (2) adding a cross-linking agent 1, 4-butanediol diglycidyl ether or genipin, and mixing; 3) carrying out a cross-linking reaction; 4) purifying a reaction product; and 5) performing vacuum freeze-drying to obtain the final composite injectable gel. According to the method, the preparation process is simplified, and the prepared composite injectable gel has significantly improved injectability, swelling ratio, mechanical strength and drug loading rate, and lower cytotoxicity, and has good application prospects in the fields of drug delivery, tissue engineering, skin wound healing and repair, medical cosmetology and the like.