Injectable temperature sensitive gel used for filling and repairing damaged tissues

A temperature-sensitive gel and damaged tissue technology, applied in medical science, prosthesis, etc., can solve problems such as lack of thermodynamic reversibility, achieve prolonged retention time, low heavy metal content, and promote in situ regeneration and repair.

Inactive Publication Date: 2012-01-18
冯淑芹
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hyaluronic acid is a biomaterial that promotes tissue growth but is not thermodynamically reversible

Method used

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  • Injectable temperature sensitive gel used for filling and repairing damaged tissues
  • Injectable temperature sensitive gel used for filling and repairing damaged tissues
  • Injectable temperature sensitive gel used for filling and repairing damaged tissues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Preparation of active aldehyde hyaluronic acid:

[0037] Mix the raw material hyaluronic acid and deionized water according to the ratio of 0.01g / 100ml, add 0.1M, 100mL sodium periodate aqueous solution, stir and react in the dark at room temperature, add ethylene glycol solution, stir and react at room temperature, and prepare Get aldehyde hyaluronic acid;

[0038] (2) soaking, washing, and extracting the prepared gel with absolute ethanol to remove unreacted monomers and other impurities, then dialysis and purification of the solution, and freeze-drying to obtain a pure gel product;

[0039] (3) Preparation of thermosensitive chitosan:

[0040] Dissolve 0.2 g of CS in 1% acetic acid solution, slowly add 0.2 g of N-isopropylpropionamide (NIPAAm) and 4 mg of potassium persulfate (KPS), stir and dissolve at room temperature, and then blow in nitrogen to stir the reaction. The above reactant was precipitated with acetone and filtered. Vacuum drying to obtain a gel ...

Embodiment 2

[0045] (1) Preparation of hyaluronic acid with active aldehyde groups:

[0046] Mix the raw material hyaluronic acid and deionized water according to the ratio of 1g / 100ml, add 0.5M, 5mL sodium periodate aqueous solution, stir and react at room temperature in the dark, add ethylene glycol solution after the reaction, stir and react at room temperature . Prepare hyaluronic acid gel;

[0047] (2) soaking, washing, and extracting the prepared gel with absolute ethanol to remove unreacted monomers and other impurities, then dialysis and purification of the solution, and freeze-drying to obtain a pure gel product;

[0048] (3) Preparation of thermosensitive chitosan:

[0049] Dissolve 0.2 g of chitosan in 5% acetic acid solution, slowly add 2 g of N-isopropylpropionamide (NIPAAm) and 4 mg of azobisisobutyronitrile, stir and dissolve at room temperature, and then feed nitrogen to stir the reaction. The above reactant was precipitated with acetone and filtered. Vacuum drying to o...

Embodiment 3

[0054] (1) Preparation of hyaluronic acid with active aldehyde groups:

[0055] Mix the raw material hyaluronic acid and deionized water according to the ratio of 20g / 100ml, add 10M, 100ml sodium periodate aqueous solution, stir and react at room temperature in the dark, add ethylene glycol solution after the reaction, stir and react at room temperature. Prepare hyaluronic acid gel;

[0056] (2) soaking, washing, and extracting the prepared gel with absolute ethanol to remove unreacted monomers and other impurities, then dialysis and purification of the solution, and freeze-drying to obtain a pure gel product;

[0057] (3) Preparation of thermosensitive chitosan:

[0058] Dissolve 0.2 g of CS in 98% acetic acid solution, slowly add 18 g of N-isopropylpropionamide (NIPAAm) and 1 mg of potassium persulfate (KPS), stir and dissolve at room temperature, and then blow in nitrogen to stir the reaction. The above reactant was precipitated with acetone and filtered. Vacuum drying t...

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Abstract

The invention relates to an injectable temperature sensitive gel used for filling and repairing damaged tissues. The gel is characterized in that: medical-grade hyaluronic acid and chitosan are used as gel raw materials; the gel swelling ratio is 2-500; and a temperature sensitive temperature is 32-37 DEG C. The gel provided by the invention has the advantages that the injection temperature sensitive gel has physical and chemical properties of a general gel, also has injectable and temperature sensitive properties and a good fluidity at a room temperature and is easy for injection; the formability in organisms is good and the degradation is slow so that the general gel can not be compared; a cross-linking agent is not used for the gel so that harms of the cross-linking agent to a human body are avoided; a gel system can prolong a persistence time of the gel in a body, and also can continuously stimulate the body to produce reactions so that the tissue in-situ regeneration and repair is promoted and the filling quality is increased; and the gel simultaneously has the advantages of reducing bacterial invansion, anticoagulation and low allergic reaction and the like.

Description

technical field [0001] The invention relates to a preparation method of an injectable temperature-sensitive gel used for filling and repairing damaged tissues, and belongs to the technical field of medical material manufacturing methods. Background technique [0002] Injectable filling and repair materials are a kind of intelligent polymer materials, which is a relatively new research field developed in the past ten years, and it is a very popular research topic of intelligent polymer materials. Compared with conventional injectable materials, temperature-sensitive injectable materials use the principle of bionics to develop temperature-sensitive polymer materials whose functions are close to or even exceed those of biological tissues, based on the organisms in nature. Thus realizing the transition from biologically inactive biological material to biologically active biological material; increasing its response sensitivity and improving its on-off characteristics. The therm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G81/00C08B37/08C08F251/00C08J3/075C08L5/08A61L27/20A61L27/52A61L27/50
Inventor 冯淑芹
Owner 冯淑芹
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