Method for removing aflatoxin out of peanut oil

A technology of aflatoxin and peanut oil, applied in the field of biochemistry, can solve the problems of harsh enzyme action conditions, achieve mild reaction conditions, avoid large oil consumption, and have broad application prospects

Active Publication Date: 2014-06-25
山东金胜粮油食品有限公司
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AI-Extracted Technical Summary

Problems solved by technology

Although the research on the biodegradation of aflatoxins has made great progress, there is still a ce...
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Abstract

The invention provides a method for removing aflatoxin out peanut oil. The method comprises the following steps: A, selecting materials; B, grading and screening peanuts; and C, feeding and squeezing oil, The method is characterized in that aflatoxin is removed by means of a compound enzyme in the step C, wherein the compound enzyme consists of phospholipase A1, laccase, neutral proteases, xylanases by adding phospholipase A1, laccase, neutral proteases and xylanases respectively according to proportions of 0.3g/kg, 0.2g/kg, 0.5g/kg and 0.1g/kg; then hydrolysis is carried out at 42 DEG C for 5-6 hours; and finally the pH of hydrolyzate is adjusted to 6.8. According to the method, aflatoxin is removed by a biological method, reaction conditions are mild and operation is simple, aflatoxin content meets national standards, and the method disclosed by the invention has a broad application prospects.

Application Domain

Fatty-oils/fats production

Technology Topic

ProteasesNeutral protease +8

Image

  • Method for removing aflatoxin out of peanut oil
  • Method for removing aflatoxin out of peanut oil

Examples

  • Experimental program(3)

Example Embodiment

[0029] Example 1
[0030] A method for removing aflatoxin in peanut oil, comprising
[0031] A Material selection process:
[0032] First, distinguish the origin of the raw materials, choose high-quality peanuts as a batch of 200 tons, and conduct sampling inspections before they are shelled and changed into rice, such as: water impurities, aspergillus flavus, acid value, peroxides, oil content and other indicators for laboratory testing , Make a record; after passing the test, it will be accepted and put into storage.
[0033] Use a specific gravity stone remover to separate and clean the dust, earth and rocks, metals, impurities, etc. in the fruit; use a hulling machine to separate large and small fruits according to specific gravity levels to reduce the damage of peanuts. Ensure that the damage rate is around 5%, and put the changed semi-finished peanuts into the warehouse.
[0034] B Peanut Grading Screening:
[0035] Firstly, check the opening of the bag, arrange the raw material bags in order and pick them out. The poor ones are picked out and processed separately, and the ones that meet the standards are upgraded to the screening workshop for grading and screening; magnetic drums are installed at the material openings; to prevent the inflow of metal and screen The bottoms are stored separately, and the rice on the sieve is divided into the storage hopper of the selection workshop. The flow rate of the material inlet is controlled by a manual switch to flow into the raw material conveyor belt of the hand sorting workshop. The material layer and magnets are installed at the lead, and the hand picking conveyor belt starts to drive After that, the general material layer is a peanut thick and uniform, which is convenient for hand picking. The conveyor belt is generally 12 meters long and 1 meter wide. Each workshop has 6 belts, and each belt is arranged on both sides There are 10-20 skilled pickers, and the number of people can be determined according to the raw material. The buds, molds, petals, heterochromatic particles, imperfect particles, oil-returned particles, and soil and rocks are sorted out and stored separately; The raw materials are not damaged, will not be oxidized by the air, and have high freshness, which can completely retain the antioxidant value of peanuts themselves, so that the peroxide value and acid value of peanut oil are stable during storage; the problem of nutrient loss is completely solved.
[0036] Feeding and oil extraction:
[0037] (1) Pretreatment: peanut raw material and 20% (mass fraction) citric acid aqueous solution are mixed at a weight ratio of 1:5, soaked at 50°C for 40 minutes, then coarsely crushed with a tissue crusher, and then finely crushed with a colloid , And pass through a 50-80 mesh sieve to remove large particles and fibers to obtain a peanut raw material pretreatment mixture. This can prevent them from sinking to the bottom of the reactor and overheating burnt to produce peculiar smell during the subsequent thermal reaction.
[0038] (2) Mix the peanut raw material pretreatment mixture obtained in step (1) with the compound enzyme to obtain the peanut raw material hydrolysate. The compound enzymes are: phospholipase A1, laccase, neutral protease, and xylanase according to 0.3 g/kg (peanut raw material pretreatment mixture), 0.2g/kg, 0.5g/kg, 0.1g/kg, and then hydrolyzed at 42°C for 5 hours to adjust the pH of the hydrolysate to 6.8;
[0039] (3) After the reaction, the temperature is raised to 120°C to inactivate the enzyme, and then 3% (V/V) boiling water is added to stir to make the gum water swell and separate from the oil. Finally, the gum is separated by centrifugation at 10000 r/min for 10 min ;
[0040] (4) Peanut oil is obtained through heat treatment-pressing-crude oil-filtration.

Example

[0041] Comparative Example 1: The material selection process and the peanut grading and screening process are exactly the same as those in Example 1. The feeding and oil extraction process is different, and the steps (2) and (3) are not included, that is, no compound enzyme preparation is added.
[0042] The experimental results are shown in Table 1 The treatment effect of compound enzyme on aflatoxin:
[0043] Table 1
[0044]

Example Embodiment

[0045] Example 2
[0046] A method for removing aflatoxin in peanut oil, comprising
[0047] A Material selection process:
[0048] First, distinguish the origin of the raw materials, choose high-quality peanuts as a batch of 200 tons, and conduct sampling inspections before they are shelled and changed into rice, such as: water impurities, aspergillus flavus, acid value, peroxides, oil content and other indicators for laboratory testing , Make a record; after passing the test, it will be accepted and put into storage.
[0049] Use a specific gravity stone remover to separate and clean the dust, earth and rocks, metals, impurities, etc. in the fruit; use a hulling machine to separate large and small fruits according to specific gravity levels to reduce the damage of peanuts. Ensure that the damage rate is around 5%, and put the changed semi-finished peanuts into the warehouse.
[0050] B Peanut Grading Screening:
[0051] Firstly, check the opening of the bag, arrange the raw material bags in order and pick them out. The poor ones are picked out and processed separately, and the ones that meet the standards are upgraded to the screening workshop for grading and screening; magnetic drums are installed at the material openings; to prevent the inflow of metal and screen The bottoms are stored separately, and the rice on the sieve is divided into the storage hopper of the selection workshop. The flow rate of the material inlet is controlled by a manual switch to flow into the raw material conveyor belt of the hand sorting workshop. The material layer and magnets are installed at the lead, and the hand picking conveyor belt starts to drive After that, the general material layer is a peanut thick and uniform, which is convenient for hand picking. The conveyor belt is generally 12 meters long and 1 meter wide. Each workshop has 6 belts, and each belt is arranged on both sides There are 10-20 skilled pickers, and the number of people can be determined according to the raw material. The buds, molds, petals, heterochromatic particles, imperfect particles, oil-returned particles, and soil and rocks are sorted out and stored separately; The raw materials are not damaged, will not be oxidized by the air, and have high freshness, which can completely retain the antioxidant value of peanuts themselves, so that the peroxide value and acid value of peanut oil are stable during storage; the problem of nutrient loss is completely solved.
[0052] Feeding and oil extraction:
[0053] (1) Pretreatment: peanut raw materials and 20% (mass fraction) citric acid aqueous solution are mixed at a weight ratio of 1:7, soaked at 60°C for 50 minutes, then coarsely crushed with a tissue crusher, and then finely crushed with a colloid And pass through a 50-80 mesh sieve to remove large particles and fibers to obtain a peanut raw material pretreatment mixture. This can prevent them from sinking to the bottom of the reactor and overheating burnt to produce peculiar smell during the subsequent thermal reaction.
[0054] (2) Mix the peanut raw material pretreatment mixture obtained in step (1) with the compound enzyme to obtain the peanut raw material hydrolysate. The compound enzymes are: phospholipase A1, laccase, neutral protease, and xylanase according to 0.3 g/kg (peanut raw material pretreatment mixture), 0.2g/kg, 0.5g/kg, 0.1g/kg, and then hydrolyzed at 42℃ for 6 hours to adjust the pH of the hydrolysate to 6.8;
[0055] (3) After the reaction, the temperature is raised to 120°C to inactivate the enzyme, and then 3% boiling water is added to stir to make the colloid water swell and separate from the oil. Finally, the colloid is separated by centrifugation at 10 000 r/min for 10 min;
[0056] (4) After heat treatment-conventional operation to press oil.
[0057] Peanut oil quality determination method Aflatoxin B1 determination method according to the national standard "Determination of aflatoxin B1 in food" GB/T5009.22-2003; results in the peanut oil prepared in Example 2 the content of aflatoxin B1 in 12.7μg/kg .
[0058] All indicators of peanut oil quality have reached the national first-class peanut oil indicators (Table 2), and all national standards for aflatoxin B1 content are in line with QS certification standards. Table 2: Example 2 Preparation of peanut oil quality sampling inspection results.
[0059] Table 2
[0060]

PUM

no PUM

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