181results about How to "Guaranteed biocompatibility" patented technology

A preparation method of a grapheme/hydroxyapatite composite material comprises the following steps: uniformly mixing oxidized grapheme with the concentration of 0.5-5 mg/ml and a solution or a turbid liquid with the calcium ion concentration of 0.01-3.0 mol/L to obtain a first mixed solution; adding a solution I with the phosphate anion concentration of 0.01-2.0 mol/L to the first mixed solution according to the molar ratio of calcium to phosphorus is (1.5-2.0) to 1, and uniformly mixing the solution I and the first mixed solution under the action of magnetic mixing; adjusting the pH to be 8-14 through a pH adjusting agent to obtain a second mixed solution; transferring the second mixed solution to a reaction kettle; carrying out hydro-thermal treatment for 1-12 hours at the temperature of 160-240 DEG C; cooling the second mixed solution until the temperature of the second mixed solution is the room temperature; cleaning, freezing and drying to obtain the grapheme/hydroxyapatite composite material. The preparation method is environment-friendly, simple and beneficial to large-scale production. The prepared grapheme/hydroxyapatite composite material has the advantages of good mechanical property as well as high electrical conductivity, adsorbability, biocompatibility and osteogenic activity.

A fluorescently-labeled hydrophobic modified oligochitose polymer used for preparing target medicine or fluorescent signal amplifier carrier is prepared through reaction between aqueous solution of oligochitose, fatty acid and cross-linking coupler, and reacting on isothiocyano fluorescent larbel in ice bath, darc and magnetic stirring conditions to obtain said polymer, and ultrasonically dispersing it in water to obtain its colloidal microparticles.

The invention discloses a preparation method of 3D printable iron ion double-crosslinked alginate-polyacrylamide acrylic acid high-performance hydrogel. The method comprises the following steps: firstly, evenly mixing sodium alginate, acrylamide, acrylic acid and a photo-initiator; pre-molding by using a mould or a 3D printing way; illuminating by using an ultraviolet lamp so as to realize copolymerization between an AAc monomer and an AM monomer; after the polymerization is completed, soaking a hydrogel scaffold into an Fe3<+> solution to form ionic cross-linking; finally, soaking the productinto deionized water for balancing so as to remove the unreacted monomers. Two networks are both Fe3<+> cross-linked, so that a common cross-linking point exists between the two networks, and the hydrogel is enabled to have excellent performance. In addition, the sodium alginate has good natural high polymer with good biocompatibility, and the viscosity is adjusted by adding a small amount of adhesive in a sol state, so that the hydrogel has good anti-collapse and rapid gelation characteristics; the hydrogel can be prepared into high porosity hydrogel with various complex structures by usinga 3D printing technology.

The invention relates the method for preparing rich silver antibiotic film used for medical pyrolytic carbon and TiN film. It solves the antibiosis question. The method comprises the following steps: on the pyrolytic carbon: a buffing and cleaning; b evacuating; c sputtering and cleaning for 5 minutes with 1keV nitrogen ion; d injecting silver ion: 70, 30keV energy, 2-8mA beam current, 2X1014/cm2-3X1014/cm2 dosage, and forming rich silver antibiotic film and antibiosis ratio being 100%; on TiN film: a buffing and cleaning; b evacuating; c sputtering and cleaning for 5 minutes with 1keV nitrogen ion; d sputtering Ti target with 3keV, 80mA Ar+ ion, 20-40keV, beam current: bombarding with 2-8mA high-energy N+ ion for 10-20 minutes; 50-350eV, beam current: bombarding with 10-30mA low-energy N+ ion for 60-180 minutes to deposit, N2 air pressure being 8X10 3Pa; e injecting silver ion: 30-80keV, beam current: 2-8mA, 1X1017/cm2-5X1018/cm2 dosage, forming rich silver antibiotic film and antibiosis ratio being 90%. The film has the advantages of good abradability, strong adhesive force and anti-corrosion, good cell compatibility and long antibiosis time-effect. The film can be used to prepare artificial heart valve and hard tissue alternate material which is implanted in human.

The invention belongs to the technical field of nano-imprinting and spectra, and in particular relates to a multilayer structure surface enhanced Raman scattering base and a preparation method thereof. The surface enhanced Raman scattering base is simple in process, high in efficiency, high in enhancement factor and biocompatible. The base consists of a substrate and a periodic nano columnar structure which is positioned on the substrate, wherein the nano columnar structure is a multilayer structure; the multilayer structure consists of alternation layers and a gold layer; the alternation layers consist of silver and media; the gold layer is positioned on the topmost layer. A nano-imprinting technology is used as a core technology, and a reactive ion etching process, a metal evaporation process, a silicon dioxide plating process, a metal peeling process and the like are combined to prepare a multilayer nano structure, so that the technical problems that a silver structure base is not biocompatible and a gold structure base is low in enhancement factor are solved; on the basis of ensuring biocompatibility, the enhancement factor of the base is greatly improved, the detection is efficient and sensitive, and the base can be applied to biological detection after further treatment.

The invention discloses a preparation method of a colloidal solution of nano-ZnO. The method comprises the following steps: mixing an aqueous modified cellulose solution having a concentration of 0.001-100g/L with a zinc salt solution having a concentration of 0.01-100g/L according to a volume ration of 1:10-100:1, wherein the modified cellulose is dialdehyde hydehydroxypropyl methyl cellulose obtained through the selective oxidation of the secondary hydroxyl groups in the C2 and C3 positions of hydroxypropyl methyl cellulose; and slowly adding an alkaline solution for adjusting the pH value to 8.5-11.5, and stirring at 20-90DEG C for 1-600min until reaction completion in order to obtain the colloidal solution of nano-ZnO having an average particle size of 1-100nm. The prepared nano-ZnO has the advantages of ultrahigh stability, antibiosis and ultraviolet resistance, good biocompatibility and good biodegradability, and can be applied to the coat field, the textile field, the medical health field and the like.

The invention discloses a method for preparing ordered ferriferrous oxide/chitosan nanometre composite material comprising the following steps, making chitosan solution from chitosan powder with thin acid solution, charging FeCl2 and FeCl3 solution into the chitosan solution, forming ferriferrous oxide / chitosan precursor solution, loading the precursor solution into a mould with a layer of pure chitosan film internally, placing into freeze liquid, removing the mould and applying magnetic field onto the two ends of the gel composite material for material forming, flushing to neutral with distilled water and drying.

The invention relates to a preparing method of a heterogeneous acellular dermal matrix substrate with good biocompatibility. The method concretely comprises the following steps of (1) split thickness skin graft preparation; (2) epidermis and dermis separation; (3) acellular processing; (4) cross linking; (5) virus inactivation; (6) refining; (7) Co-60 irradiation sterilization. The ecellular tissue engineering materials obtained by the method provided by the invention has the advantages that the toxicity is stable, and the cell compatibility is better.

The invention relates to a ligament support containing silk fibroin compound oriented nanofiber and a preparation method thereof, and belongs to the technical field of medical apparatus and instruments. The ligament support is prepared from oriented fiber combining silk fibroin with a medical high molecular synthetic material and has a porous three dimensional structure, the mass ratio of the silk fibroin and medical high molecular synthetic material in the compound oriented fiber is (1-20):(1-1), the diameter of the compound oriented fiber is 500nm-10000nm, the thickness of the ligament is 1mm-10mm, and the width of the ligament is 1mm-15mm. The ligament support has a uniform microstructure and good biocompatibility, provides a matrix structure for growing of ligament cells, and can be used for increasing the tensile strength of the ligament due to the compound fiber orientated structure. The ligament support is simple in preparation method and convenient to operate, the preparation process is controllable, and the preparation method is suitable for preparing the ligament supports of various sections and lengths.

The invention discloses a method for preparing an anticoagulant composite coating. The method for preparing the anticoagulant composite coating comprises the steps of first using a coupling agent to process the surface of a pre-processed biomedical base material to obtain a hydrophilic surface, using a specific anticoagulant composite coating to modify the biomedical base material, and obtaining a biomedical material having good anticoagulation property after drying. Studies have shown that the biomedical material modified by the anticoagulant composite coating is greatly improved in hydrophilicity and shows excellent biocompatibility. The modified material can not only maintain its original physical and mechanical properties, but also greatly extend activated partial thromboplastin time (APTT) in the coagulation test to show excellent anticoagulant properties.

The invention relates to a tendon reinforcing repairing material and a preparation method of the tendon reinforcing repairing material. The repairing material takes animal original dermis as the raw material and is prepared by steps of pretreatment, preparation of a holder layer, preparation of a mechanical reinforcing layer, and preparation of a tendon reinforcing repairing material. The tendon reinforcing material comprises the holder layer and the mechanical reinforcing layer, wherein the holder layer reserves the integrity of the structure and component of an extracellular matrix to the greatest degree, and due to the introduction of the mechanical reinforcing layer, the acellular dermal matrix has better mechanical property compared with the acellular dermal matrix prepared by the traditional technology, for the biocompatibility, mechanical strength and biological activity are improved and balanced. According to the requirements of different indications on materials, the preparation method can prepare membrane materials and curl materials in different thicknesses. The tendon reinforcing repairing material is widely applicable to repairing of acute and old tendon injury.

Bio-medical implanting material with biomimetic nano film and its use are both disclosed herewith. The implanting material uses NiTi shape-memory alloy as base with biomimetic nano TiO2 bio-ceramic film of 100-200nm to stop Ni ions liberated in human body liquid. It can be used as implanting device, e.g. tubular support, bone fixer, bone orthotic device, and oral orthodontic material, etc.

The invention provides a degradable bioactivity composite containing calcium phosphate and a preparation method thereof. The composite consists of calcium phosphate and a multiple amino acid polymer; wherein, the weight percentage of inorganic composition is not more than 50 percent and the residual substance is the multiple amino acid polymer which is formed by the polymerization of caprolactam and at least 5 other amino acids; wherein, the molar ratio of the caprolactam in the polymer is 40-90 percent and the respective molar ratio of other single amino acid is not less than 0.5 percent. During preparation, the caprolactam is mixed with all amino acids and dehydration is carried out under the protection of inert gases and stirring, and pre-polymerization composition reaction and polymerization reaction are respectively carried out at the temperature of 210-250DEG C, thus obtaining the composite. The indexes of the prepared material such as mechanic performance, degradation period and the like can be adjusted and controlled according to the use requirements.

The invention discloses a nanogel scaffold material for culturing and desorbing cells and a preparation method thereof. The nanogel scaffold material is formed by selfassembling low-molecular weight gel factors G1 and G2. The structural formula of the low-molecular weight gel factors is disclosed in the specification, wherein R is disclosed in the specification; when R<*> is disclosed in the specification, the low-molecular weight gel factor is G1, and when R<*> is disclosed in the specification, the low-molecular weight gel factor is G2. The preparation method of the material comprises the following steps: preparing the low-molecular weight gel factors G1 and G2; and regulating the temperature or pH value of the solvent of the low-molecular weight gel factors G1 and G2 to form the nanogel scaffold material for culturing and desorbing cells by selfassembling. The method overcomes the defect of damage to cell functions in the existing enzymolysis method and wide-range temperature change induced cell desorption, and is beneficial to adhesion, spreading and proliferation of cells on the biological material surface.

The invention belongs to the technical field of functional high polymer materials, and particularly relates to a polyurethane foam modifying method. The polyurethane foam modifying method comprises the following steps: first step, uniformly dipping a polyurethane foam which is cleaned thoroughly in a multifunctional polyurethane prepolymer solution, wherein the use amount of the multifunctional polyurethane prepolymer solution is 5-50 times the mass of the polyurethane foam; and second step, taking out the polyurethane foam which has been saturated and adsorbs the multifunctional polyurethaneprepolymer solution, after squeezing the polyurethane foam to be dry, placing the polyurethane foam in an oven, and drying the polyurethane foam for 2-72 hours at the temperature of 20-80 DEG C to obtain the multifunctional polyurethane prepolymer modified polyurethane foam. The hydrophilicity of the obtained polyurethane foam is greatly improved, and saturation water absorbing ability and water retention ability are enhanced obviously. The mechanical property, the biocompatibility and the stability of the polyurethane foam can still be maintained, and meanwhile, the polyurethane foam furtherhas an antibacterial property.

The invention discloses a method for preparing a cornea lamina material. The method comprises the following steps of: carrying out accellular processing on a cornea-sclera complex of a mammal eyeball, culturing an amnion epithelial stem cell, inducing and secreting TSP (Thrombospondin)-1 and co-culturing accellular cornea stroma and the amnion epithelial stem cell, sizing, packaging and sterilizing, and the like. According to an obtained cornea lamina transplanting material, the promotion and the balance among the biocompatibility, the stability, the transparency, the mechanical strength and the bioactivity are realized; the integrity of cornea collagen molecules and a lamina structure can be kept to the largest extent, and compared with that prepared with the traditional method, the accellular cornea stroma prepared according to the method disclosed by the invention is larger in tensile strength and has the moisture content and the transparency close to those of normal cornea; and various cell growth factors and the TSP-1 are gathered, and the cornea lamina material has special effects of inflammatory resistance and vascularization resistance, has the capability of greatly enhancing the bioactivity of the cornea lamina material and can be widely used for repairing complicated ocular surface coloboma such as inflammation and corneal ulcer grown in a vascularization way.

The invention provides a Mg alloy for being implanted in bone. The Mg alloy is characterized by comprising the following chemical components in percentage by weight: 1.5-3% of Zn, 0.6-1.1% of Mn, 0.3-0.8% of Ca, 0.3-0.45% of Si, 0.45-0.8% of Sr, 1-1.8% of Li, 2.5-3.5% of Cr, 1-2% of Cu, 1.3-1.4% of Mo, 0.01-0.08% of Sc, La and Ce, 0.1-0.8% of Nb, and the balance of Mg and inevitable impurity elements. According to the Mg alloy provided by the invention, the yield strength is higher than or equal to 370 MPa, the tensile strength is higher than or equal to 410 MPa, the elongation is higher thanor equal to 8.5%, and the sterilizing rate is higher than or equal to 90%. The above indices of the Mg alloy are obviously higher than those of other products which lack alloy elements and those of similar products which are not subjected to surface coating treatment.

The invention discloses a preparation method of antibacterial composite hydrogel, which comprises the following steps of: dissolving a water-soluble gel base material in deionized water to obtain a base material solution; dissolving modified hyaluronic acid in the base material solution to obtain a mixed solution; and uniformly dispersing silver nanoparticles in the mixed solution, and performing curing under ultraviolet irradiation by virtue of a photoinitiator, so as to obtain the antibacterial composite hydrogel. The composite hydrogel disclosed by the invention has good biocompatibility, antibacterial property, biodegradability and mechanical property, and can be used for wound dressing.