Modified sodium hyaluronate gel for injection

A technology of sodium hyaluronate and hyaluronic acid, used in medical science, prosthesis, surgery, etc., to enhance stability and cohesion, maintain biocompatibility, and achieve good filling effects

Active Publication Date: 2014-10-08
BLOOMAGE BIOTECHNOLOGY CORP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] In order to solve the problem that the gel prepared by the above prior art cannot have the advantages of less amount of cross-linking agent, good plasticity and easy injection, good stability and retention in vivo In order to solve the problem of advantages such as long time, the present invention provides a modified hyaluronic acid for injection with less dosage of cross-linking agent, less pushing force, good plasticity, good enzyme resistance, good stability and long retention time in vivo. Sodium Acid Gel

Method used

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  • Modified sodium hyaluronate gel for injection
  • Modified sodium hyaluronate gel for injection
  • Modified sodium hyaluronate gel for injection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] This embodiment investigates the influence of gel X with different crosslinking degrees on the properties of the gel of the invention. The preparation method is as follows:

[0053] (1) Dissolve 5.0 g of hyaluronic acid in 50 ml of NaOH solution (pH>10), mix well, add cross-linking agent 1,4-butanediol diglycidyl ether, cross-linking agent and hyaluronic acid The acid quality ratio is shown in Table 1, stir and mix well, and react in a water bath at 35°C for 10 h to obtain gel X;

[0054] (2) Dissolve 5.0 g of hyaluronic acid in 50 ml of NaOH solution (pH>9), mix well, add 1,4-butanediol diglycidyl ether, the mass ratio of crosslinking agent to hyaluronic acid is 0.06%, stir and mix well, and react in a water bath at 35°C for 8 hours to obtain gel Y;

[0055] (3) Mix gel X and gel Y at a ratio of 50% X-50% Y (50 g : 50 g), adjust the pH to 2~4, add 1,4-butanediol diglycidol Ether, the mass ratio of cross-linking agent to hyaluronic acid is 0.8%, react in a water bath ...

Embodiment 2

[0065] This embodiment investigates the influence of gel Y with different crosslinking degrees on the properties of the invented gel, and the preparation method is as follows:

[0066] (1) Dissolve 5.0 g of hyaluronic acid in 50 ml of NaOH solution (pH>10), mix well, add cross-linking agent 1,4-butanediol diglycidyl ether, cross-linking agent and hyaluronic acid The acid mass ratio was 2.5%, stirred and mixed, and reacted in a water bath at 35°C for 10 h to obtain gel X;

[0067] (2) Dissolve 5.0 g of hyaluronic acid in 50 ml of NaOH solution (pH>9), mix well, and then add 1,4-butanediol diglycidyl ether. 2, stir and mix well, and react in a water bath at 35°C for 8 h to obtain gel Y;

[0068] (3) Mix gel X and gel Y at a ratio of 50% X-50% Y (50 g : 50 g), adjust the pH to 2~4, add 1,4-butanediol diglycidol Ether, the mass ratio of cross-linking agent to hyaluronic acid is 0.8%, react in a water bath at 35°C for 6 h;

[0069] (4) Adjust the pH value of the gel to neutral w...

Embodiment 3

[0076] Compare the amount of crosslinker after mixing gel X and gel Y:

[0077] (1) Dissolve 5.0 g of hyaluronic acid in 50 ml of NaOH solution (pH>10), mix well, add 1,4-butanediol diglycidyl ether, the mass ratio of crosslinking agent to hyaluronic acid is 2.5%, stirred and mixed, and reacted in a water bath at 35°C for 10 h to obtain gel X.

[0078] (2) Dissolve 5.0 g of hyaluronic acid in 50 ml of NaOH solution (pH>9), mix well, add 1,4-butanediol diglycidyl ether, the mass ratio of crosslinking agent to hyaluronic acid is 0.06% (w / w), stirred and mixed, and reacted in a water bath at 35°C for 8 h to obtain gel Y.

[0079] (3) Mix gel X and gel Y at a ratio of 50% X-50% Y (50 g : 50 g), adjust the pH value to 2~4, add different ratios of 1 as shown in Table 3, 4-Butanediol diglycidyl ether was reacted in a water bath at 35°C for 6 h.

[0080] (4) Adjust the pH value of gel X to neutral with 0.5% NaOH solution, and then dialyze the gel in phosphate buffer with neutral pH...

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Abstract

The invention relates to the technical field of sodium hyaluronate gel and particularly relates to modified sodium hyaluronate gel for injection. A method for preparing the modified sodium hyaluronate gel for injection comprises the steps: enabling hyaluronic acid and a cross-linking agent to react under the condition that the pH is greater than 10, so as to obtain gel X; enabling hyaluronic acid and a cross-linking agent to react under the condition that the pH is greater than 9, so as to obtain gel Y; and mixing the gel X and the gel Y, carrying out cross-linking reaction again, adjusting the pH value to neutral and dialyzing, thereby obtaining the modified sodium hyaluronate gel for injection. The modified sodium hyaluronate gel for injection, disclosed by the invention, is efficient cross-linked gel, has enhanced stability and cohesiveness, has excellent rheological properties and is good in shaping performance, easy in injection, long in in-vivo retention time and good in filling effect, the dosage of the cross-linking agent is small, and the biocompatibility can still be maintained.

Description

technical field [0001] The invention relates to the technical field of sodium hyaluronate gel, in particular to a modified sodium hyaluronate gel for injection. Background technique [0002] Soft tissue fillers in non-surgical cosmetology are widely favored by people, and the amount of use is second only to botulinum toxin. It uses biocompatible filler materials and is injected into the skin to increase the volume of soft tissues and achieve wrinkle removal or shaping. Purpose. An ideal soft tissue filler should have the following properties: (1) long-lasting effect; (2) easy to inject; (3) flexible / plastic. [0003] The most widely used polysaccharide for medical purposes is hyaluronic acid (HA). Hyaluronic acid is a straight-chain polymer mucopolysaccharide composed of glucuronic acid and acetylglucosamine disaccharide units, which widely exists in humans, animals and microorganisms, without species differences, non-toxic, and non-immune sources Non-irritating, non-ir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24A61L27/20A61L27/54A61L27/52A61L31/04A61L31/16
Inventor 张燕刘建建杨莹莹李超郭学平
Owner BLOOMAGE BIOTECHNOLOGY CORP LTD
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