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Fluorescence labeling hydrophobic modified chitin polymer, its preparation method and application

A hydrophobic modification and fluorescent labeling technology, which is applied in the direction of pharmaceutical formulations, medical preparations of non-active ingredients, etc., can solve the problems of high viscosity and large molecular weight, and achieve the effect of simple method, mild reaction conditions and easy control

Inactive Publication Date: 2006-01-11
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commercially available chitosan has the disadvantages of large molecular weight (hundreds of thousands), high viscosity, and inability to dissolve under physiological pH (7.4) conditions.

Method used

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  • Fluorescence labeling hydrophobic modified chitin polymer, its preparation method and application
  • Fluorescence labeling hydrophobic modified chitin polymer, its preparation method and application
  • Fluorescence labeling hydrophobic modified chitin polymer, its preparation method and application

Examples

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Effect test

example 1

[0027] 1. Preparation of chitosan oligosaccharide

[0028] Add 6g of chitosan (average molecular weight 450kDa) to 200mL of 1.25% (v / v) hydrochloric acid aqueous solution, stir and dissolve at 55-60°C, adjust the pH to 5.0 with dilute ammonia water or dilute hydrochloric acid, press cellulase and shell The ratio of polysaccharides is 0.5:100 (w / w) to add cellulase, and after controlling the reaction time for 8 hours, the reaction product is 4000r×min -1 After centrifugation for 10 minutes, the supernatant was pretreated with a 0.45 μm microporous membrane, graded by ultrafiltration with ultrafiltration membranes of different molecular weights, and the ultrafiltrate was freeze-dried to obtain chitosan oligosaccharides with a certain molecular weight. And the average molecular weight was determined to be 50.6 kDa by gel permeation chromatography.

[0029] 2. Preparation of fluorescently labeled hydrophobic modified chitosan oligosaccharides

[0030] Get above-mentioned chitosa...

example 2

[0037] 1. Preparation of chitosan oligosaccharide

[0038] Add 6g of chitosan (average molecular weight 450kDa) to 200mL of 1.25% (v / v) hydrochloric acid aqueous solution, stir and dissolve at 55-60°C, adjust the pH to 5.0 with dilute ammonia water or dilute hydrochloric acid, press cellulase and shell Add cellulase at a glycan ratio of 0.5:100 (w / w), and control the reaction time for 24 hours. -1 After centrifugation for 10 minutes, the supernatant was pretreated with a 0.45 μm microporous membrane, graded by ultrafiltration with ultrafiltration membranes of different molecular weights, and the ultrafiltrate was freeze-dried to obtain chitosan oligosaccharides with a certain molecular weight. And the average molecular weight was determined to be 10.1 kDa by gel permeation chromatography.

[0039] 2. Preparation of fluorescently labeled hydrophobic modified chitosan oligosaccharides

[0040] Take 0.5g chitosan (average molecular weight 10.1kDa), accurately weighed. After ad...

example 3

[0046] 1. Preparation of chitosan oligosaccharide

[0047] Add 6g of chitosan (average molecular weight 450kDa) to 200mL of 1.25% (v / v) hydrochloric acid aqueous solution, stir and dissolve at 55-60°C, adjust the pH to 5.0 with dilute ammonia water or dilute hydrochloric acid, press cellulase and shell Add cellulase at a glycan ratio of 0.5:100 (w / w), and control the reaction time for 20 hours. -1 After centrifugation for 10 minutes, the supernatant was pretreated with a 0.45 μm microporous membrane, graded by ultrafiltration with ultrafiltration membranes of different molecular weights, and the ultrafiltrate was freeze-dried to obtain chitosan oligosaccharides with different molecular weights. And the average molecular weight was determined to be 18.8 kDa by gel permeation chromatography.

[0048] 2. Preparation of fluorescently labeled hydrophobic modified chitosan oligosaccharides

[0049] Get 0.5g chitosan oligosaccharide (average molecular weight 18.8kDa), accurately we...

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Abstract

A fluorescently-labeled hydrophobic modified oligochitose polymer used for preparing target medicine or fluorescent signal amplifier carrier is prepared through reaction between aqueous solution of oligochitose, fatty acid and cross-linking coupler, and reacting on isothiocyano fluorescent larbel in ice bath, darc and magnetic stirring conditions to obtain said polymer, and ultrasonically dispersing it in water to obtain its colloidal microparticles.

Description

technical field [0001] The invention belongs to a preparation method of a compound, and relates to a preparation method of a fluorescently labeled hydrophobically modified chitosan oligosaccharide polymer and its application in the field of life sciences as a carrier of nanometer micelles. Background technique [0002] In recent years, microparticle carriers have received more and more attention and applications in the field of drug targeting. For example, liposomes, nanoparticles, microspheres and polymer micelles have been studied more. These particulate carriers are considered to be an excellent targeted drug delivery system due to their small particle size, long-term circulation in the body, good biocompatibility, and biomembrane penetration. In particular, it has unique advantages in macromolecular drugs and gene delivery, and is a hot spot in the current research field. [0003] Drug-targeted therapeutics divides drug-targeted therapy into three stages of development...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08A61K47/36
Inventor 胡富强叶轶青袁虹
Owner ZHEJIANG UNIV
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