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Nanogel scaffold material for culturing and desorbing type of cells and preparation method thereof

A technology of cell culture and nanogel, applied in the field of biology, to achieve the effect of avoiding cell function damage

Active Publication Date: 2012-07-04
海安百优基质生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of existing natural and synthetic polymer cell culture scaffold materials, the technical problem to be solved in the present invention is to improve a new type of intelligent low molecular weight gel scaffold material for cell culture and induced cell detachment and its preparation method

Method used

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  • Nanogel scaffold material for culturing and desorbing type of cells and preparation method thereof
  • Nanogel scaffold material for culturing and desorbing type of cells and preparation method thereof
  • Nanogel scaffold material for culturing and desorbing type of cells and preparation method thereof

Examples

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Effect test

Embodiment 1

[0049] This example is an example of the preparation method of a nanogel scaffold material used for cell culture and desorption.

[0050] This embodiment provides a nanogel scaffold material for a type of cell culture and detachment, which is formed by self-assembly of low molecular weight gel factors G1 and G2;

[0051] The structural formula of the low molecular weight gel factor is:

[0052]

[0053] in: when When , the low molecular weight gel factor is G1; when When , the low molecular weight gelling factor is G2.

[0054] The nanogel scaffold material for one type of cell culture and desorption can be prepared by adjusting the solvent temperature or pH value.

[0055] The preparation method of the nanogel scaffold material used for a class of cell culture and detachment comprises the following steps:

[0056] Step 1, take 1,4-cyclohexanedicarboxylic acid, L-phenylalanine methyl ester hydrochloride, histamine, BOC-L-lysine, N,N'-carbonyldiimidazole, anhydrous...

Embodiment 2

[0074] This example is an example of reversibly regulating the self-assembly of low-molecular-weight gelling factors by pH.

[0075] Prepare three samples of gel factor G1 respectively: (a) 0.1wt% G1 aqueous solution prepared by dissolving G1 by heating and then cooling; HCl adjusts the pH value of the solution to 3.5; (c) the 0.1wt% G1 aqueous solution prepared by heating and dissolving G1 and then cooling the solution, adjusts the pH value of the solution to 2 with 1M HCl, and then adjusts the pH value of the solution to 7.4 with 2M NaOH. Immerse the mica flakes in the above samples (a), (b) and (c) respectively, take out the mica flakes after 24 hours, dry them at normal temperature and pressure, and observe with an atomic force microscope that the gel factor G1 has a very strong Self-assembly ability, network-like nanofibers can be formed by heating and cooling; the pH value of the G1 solution is adjusted to acidity, and the repulsion of the protonation of the gelling fact...

Embodiment 3

[0077] This example is an example of regulating the self-assembly of low-molecular-weight gelling factors through mild pH changes.

[0078] Prepare the G1 aqueous solutions with pH values ​​of 7.4, 7.2, 7.0, 6.5, 6.0, and 3.5 respectively, and the G1 concentration is 0.018wt%, and the G1 solutions of the above-mentioned different pH values ​​are detected by ultraviolet-visible spectroscopy ( Figure 4 ). It can be seen from the ultraviolet-visible spectrum that when the pH is 7.4, the maximum absorption peak is at 302nm, corresponding to the characteristic peak of the self-assembly of G1 gelling factor to form nanofibers; when the pH is 3.5, the maximum absorption peak is at 278nm, corresponding to the protonation The characteristic absorption peak of G1 dispersed single molecule. As the pH value of the solution decreased, the self-assembled system was destroyed, the energy of the system increased, and the absorption peak shifted blue. In particular, when the pH of the G1 so...

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Abstract

The invention discloses a nanogel scaffold material for culturing and desorbing cells and a preparation method thereof. The nanogel scaffold material is formed by selfassembling low-molecular weight gel factors G1 and G2. The structural formula of the low-molecular weight gel factors is disclosed in the specification, wherein R is disclosed in the specification; when R<*> is disclosed in the specification, the low-molecular weight gel factor is G1, and when R<*> is disclosed in the specification, the low-molecular weight gel factor is G2. The preparation method of the material comprises the following steps: preparing the low-molecular weight gel factors G1 and G2; and regulating the temperature or pH value of the solvent of the low-molecular weight gel factors G1 and G2 to form the nanogel scaffold material for culturing and desorbing cells by selfassembling. The method overcomes the defect of damage to cell functions in the existing enzymolysis method and wide-range temperature change induced cell desorption, and is beneficial to adhesion, spreading and proliferation of cells on the biological material surface.

Description

technical field [0001] The invention relates to the technical field of biology, in particular to a nanogel scaffold material and a preparation method for a type of cell culture and desorption. Background technique [0002] With the development of life science, material science and engineering, tissue engineering, an interdisciplinary field, has emerged. The core of tissue engineering is to establish a complex composed of cells and biological materials to form a living tissue with vitality, reconstruct the shape, structure and function of the diseased tissue and achieve permanent replacement; After amplification, large tissue defects are repaired; it can be shaped arbitrarily according to the defects of tissues and organs to achieve perfect shape repair. [0003] The earliest tissue engineering scaffold materials are natural matrix materials, such as collagen, fibrin, natural polysaccharides, alginate, hyaluronic acid, chitosan, etc. They have good biocompatibility, but nat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C07D233/64C07K5/065
Inventor 冯传良窦晓秋张荻
Owner 海安百优基质生物工程有限公司
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