34 results about "Nanogel" patented technology

The application belongs to the technical field of dental antibacterial materials, and discloses a kind of nanometer composite antibacterial coating for dental material and preparation method thereof.Method: monomer modified arginine, monomer N-isopropyl acrylamide, monomer methyl hexyl acrylate, initiator azobisdimethylamino propanamide hydrochloride, dispersing agent cetyltrimethylammonium bromide and crosslinking agent N,N-methylene bis(acrylamide) are dissolved in water, and the reaction is carried out under protective atmosphere, dialysis, to obtain nanogel dispersion;The nanogel dispersion is mixed with the nanometer zinc oxide dispersion to obtain a nanogel composite zinc oxide antibacterial coating, and a coating is formed after coating.The nanometer composite antibacterial coating for dental material has excellent retention capacity and antibacterial performance.

The application discloses hyperbranched nanocluster solidified isocyanate adhesive as well as a preparation method and application thereof, belongs to the field of adhesives, and specifically comprises the following components in parts by weight: 68-72 parts of polymethylene polyphenyl polyisocyanate, 28-32 parts of hyperbranched amino oligomeric siloxane nanoclusters, 3-6 parts of double-reactive hydrophobic nanogel, 1-3 parts of coupling accelerator, and 0.1-1 parts of catalyst. The application is characterized in that the hyperbranched amino oligomeric siloxane nanoclusters, the double-reactive hydrophobic nanogel and the isocyanate adhesive are synergistically compounded to form a composite adhesive layer structure with high crosslinking density and good interfacial bonding force, the water resistance and the moisture and heat stability of the adhesive layer are improved, and the obtained water-resistant shaving board has high strength, low water absorption and excellent water-resistant bonding performance.

This invention provides a formulation and preparation method for an exosome-based skin medication. Taking the preparation of a 100g finished formulation as an example, it consists of active ingredients, a nanogel carrier, a composite stabilizer, a moisturizer, a pH adjuster, a preservative, and deionized water. By constructing a nanogel sustained-release system adapted to exosomes and a composite protection mechanism, the exosomes are stably and slowly released and efficiently absorbed on the skin surface within 7 days. This solves the problems of rapid release, low utilization rate, poor stability, and frequent reapplication required for exosome-based skin medications, thereby improving the skin care and treatment effects of exosomes.

The present application relates to the field of textile materials, and discloses a method for improving the automatic regulation ability of fabric comfort by means of local plasmonic enhancement effect, comprising the following steps: (1) preparing temperature-sensitive nanogel; (2) loading gold nanoparticles in the nanogel; (3) forming a composite nanogel coating on the surface of the cotton fabric by the dipping method to obtain the fabric with the automatic regulation ability of comfort; the present application first prepares the temperature-sensitive nanogel by emulsion polymerization; then generates gold nanoparticles in the nanogel by in-situ reduction method; finally, the composite nanogel is fixed on the cotton fabric to form a composite nanogel layer on the surface of the cotton fabric; through the temperature sensitivity of the nanogel and the local plasmonic enhancement effect between the gold nanoparticles, the fabric comfort can be adjusted according to the temperature and light conditions of the external environment, and the present application is suitable for outdoor sports in daily life.

The application discloses a preparation method of a novel hierarchical drug release lemon exosome nano preparation and application thereof in brain glioma treatment and belongs to the field of nano medicines. The method comprises the following steps: extracting and purifying lemon exosomes, introducing an anti-glioma drug and a first part of LRP1 up-regulating agent into the inner cavity of the exosomes through electroporation, instantaneously constructing a tumor microenvironment responsive nanogel core to fix the drug, embedding a second part of LRP1 up-regulating agent into the lipid layer of the exosome, modifying Angiopep-2 to realize brain targeting, and obtaining the nano preparation through purification. The preparation has the hierarchical drug release characteristics of outer layer early release and inner cavity delayed release, can improve the efficiency of drug crossing the blood-brain barrier and accumulation at the tumor site, enhance the anti-glioma effect, solves the technical problem that the existing drug loading system is difficult to realize multi-drug phased release, and provides a new strategy for brain glioma treatment.

The application belongs to the technical field of high polymer substances, and particularly relates to a preparation method of astaxanthin-loaded ovomucin-pectin-epsilon-polylysine hydrochloride composite nanogel. Under stirring conditions, a pectin solution is added into an ovomucin solution; then an astaxanthin solution is added, and ultrasonic treatment is performed to obtain a mixed solution; an epsilon-polylysine hydrochloride solution is added into the mixed solution, and stirring reaction is performed to obtain a mixture; the mixture is centrifuged to obtain a dispersion liquid and a precipitate, and the dispersion liquid is dialyzed after separation to obtain the astaxanthin-loaded ovomucin-pectin-epsilon-polylysine hydrochloride composite nanogel. The application uses naturally-sourced ovomucin, pectin and epsilon-polylysine hydrochloride as raw materials, effectively avoids the use of organic solvents, and reduces the risk of organic residue; the preparation method has mild conditions and simple operation, the raw materials are widely sourced and low in price, and the application does not need complex equipment and organic solvents, and has good prospects for large-scale production and application.

The application discloses an antifungal nanogel and a preparation method and antifungal product thereof, relates to the technical field of antifungal materials, and discloses an antifungal nanogel which comprises the following preparation raw materials: a cell-penetrating peptide modified quaternary ammonium polysaccharide derivative and a polyphenol crosslinking agent. The antifungal nanogel of the application realizes the high-efficiency antifungal effect through deep penetration mediated by the cell-penetrating peptide and the synergistic effect of the quaternary ammonium polysaccharide derivative and the polyphenol.

Covalently linked linear polyethylenimine (PEI) clusters are provided that change conformation depending upon changes in counterion concentrations. The structures may be used for the storage, delivery, and / or transport of substances.

This invention discloses a plasma-activated silver nanogel, its preparation method, and its application. The preparation method includes the following steps: S1, mixing silver nanoparticles with agarose solution, heating and reacting, and then allowing it to stand until it becomes a gel; S2, immersing the gel obtained in S1 in physiological saline; S3, subjecting the gel treated in S2 to plasma treatment to obtain the plasma-activated silver nanogel. The method of this invention locks the active particles in the gel before applying it to the skin surface, avoiding direct or close-range contact between the plasma generator and the skin, thus preventing skin burns and ensuring safety. Simultaneously, it synergistically enhances the effects of silver ions and ClO₂. ‑ It has a dual antibacterial mechanism, which relieves redness, swelling, and thickened scales, accelerates the resolution of inflammation, regulates the immune response, and relieves symptoms of chronic inflammatory diseases (such as psoriasis).

The application discloses a kind of polymer and drug-loaded nanogel, the method for surface modification of drug-loaded nanogel of adoptive immune cell and application thereof, belong to the field of biological medicine.The polymer structure is as shown in formula 1. Wherein, R is selected from x, y, z, k is the degree of polymerization, 10≤x+y+z+k≤4000, x>0, y>0, z>0, k>0;N is the degree of polymerization, 10≤n≤400.The drug-loaded nanogel prepared by the polymer is used to modify the surface of adoptive immune cell, so that the adoptive immune cell has excellent tumor treatment effect.The method for surface modification of drug-loaded nanogel of adoptive immune cell is stable, simple, and has strong universality, can realize the intelligent controllable of dosing, significantly enhances the killing ability of solid tumor or in vivo tracing ability.

The application belongs to the technical field of antitumor drugs, and particularly relates to a nanofiber hydrogel for synergistic antitumor sonodynamic / chemodynamic therapy, a preparation method and application thereof, synthesis of Nap-Pept, preparation of Nap-Pept / Fe 3+ / Cur nanogel, under ultrasonic conditions, synergistic sonodynamic therapy of a traditional Chinese medicine sonosensitizer Cur and Fe 3+ mediated chemodynamic therapy, triggering immunogenic death of cancer cells by increasing ROS levels of B16F10 cells and then damaging mitochondrial function. Nap-Pept / Fe 3+ / Cur@US promotes expression of the immune-related factor calnexin, release of high mobility group protein 1 and maturation of dendritic cells to kill tumor cells, thereby exerting an antitumor effect. In vivo and in vitro studies show that the anticancer effect of the combined sonodynamic and chemodynamic therapy is superior to that of single therapy, achieving the purpose of precise and efficient anti-melanoma.

The application provides a drug-loaded gelatin nanogel, a biomimetic nanogel and a preparation method and combined drug and use thereof, and belongs to the technical field of biological medicines.The application forms a drug-loaded gelatin nanogel by reacting all-trans retinoic acid with pirfenidone and gelatin, cooperatively inhibits the activation of pancreatic stellate cells and blocks the pro-fibrosis signal pathway, realizes the reversal of the fibrosis microenvironment of pancreatic cancer, coats the drug-loaded gelatin nanogel on the membrane of activated pancreatic stellate cells to construct a biomimetic nanogel, so that the biomimetic nanogel has active targeting, and the drug delivery efficiency and the treatment effect are significantly improved, and the drug-loaded gelatin nanogel, the biomimetic nanogel and a chemotherapeutic drug are combined to treat pancreatic cancer, have a significant anti-tumor effect, and the combined treatment of the biomimetic nanogel and gemcitabine has a synergistic anti-tumor effect.The gelatin realizes effective regulation of the dense fibrosis microenvironment of pancreatic cancer, and provides a new feasible strategy for the treatment of pancreatic cancer.

The application belongs to but is not limited to the technical field of gene synthesis, and discloses a preparation method of a red blood cell membrane coated framework nucleic acid nanogel, which forms NG through base complementary pairing, and realizes the compounding of RBCm and NG by using an Avanti small extruder.SiRNA is combined and cross-linked with TDN through base complementary to form NG, and the TDN provides the first layer of protection for the siRNA, enhances the stability and drug efficacy of the siRNA, and the red blood cell membrane provides the second layer of protection to prevent enzyme degradation and protein adsorption.M@NG combines the bionics and biocompatibility of RBCm and the high drug loading and stability of NG, and has the characteristics of targeted drug delivery, immune escape and the like.The system is suitable for the treatment of various diseases, and expands the application field of RNA interference technology.

This invention relates to the field of biodetection technology, providing a logic-gated nanogel probe for live cell typing and its preparation method. The invention uses a thermosensitive polymer nanogel as a carrier, with its surface covalently modified with an antibody that specifically binds to the EGFR L858R mutant protein and a peptide nucleic acid that specifically binds to miR-21. Internally, it encapsulates a self-quenched fluorescent dye. The probe employs an AND logic gating design, triggering gel network dissociation and dye release to generate a fluorescent signal only when it simultaneously recognizes the EGFR L858R protein on the cell surface and captures miR-21 within the cell. This invention solves the problems of high false positive rates in existing single-target detection methods and the inability of traditional liquid biopsy techniques to perform simultaneous genotype-phenotype analysis in situ on live cells. It achieves highly specific and sensitive in situ interpretation of circulating tumor cells, and has significant application value in the field of precision diagnosis and treatment of lung cancer.

This invention discloses the preparation and application of radiofrequency thermoresponsive platinum ion crosslinked black phosphorus nanogel. The nanogel has a core-shell structure, comprising a core composed of a radiofrequency thermosensitive agent and chemotherapeutic drugs, and a shell constructed from a thermosensitive nanopolymer. The thermosensitive nanogel BP-Pt@PNA prepared using the above components exhibits radiofrequency thermosensitive properties. Simultaneously, the introduction of the thermosensitive nanopolymer improves the stability of the radiofrequency thermosensitive agent and chemotherapeutic drugs, enabling long-term drug retention and fully leveraging the synergistic effect of non-invasive radiofrequency thermotherapy and chemotherapy. This drug-loaded nanogel can achieve multimodal treatment applications, including non-invasive radiofrequency thermotherapy-chemotherapy synergistic antitumor therapy and invasive radiofrequency ablation-chemotherapy-vascular embolization synergistic antitumor therapy, demonstrating significant clinical translational potential.

Biologics, including peptides, proteins, antibodies, nucleic acids (DNA and RNA), oligonucleotides, vaccines, or complex combinations of these substances, are important for treating various types of diseases and tissue and organ regeneration. However, biologics are not stable and have short half-lives, making effective delivery to patients difficult. Therefore, there is an unmet need in the art to increase the stability and half-lives of biologics for long-term bioavailability, therapy, treatment and repair. This invention provides a nanogel platform technology that can load biologics in aqueous solution with high loading efficiency without using any organic solvent and also sustain the release of active biologics in the body for more than 2 months.

The present application relates to a kind of cell membrane-coated pH-responsive nanogel and its preparation method, the pH-responsive nanogel is that chitosan is crosslinked by crosslinking agent to form nanogel, then load polypeptide ENO1, and further load drug sunitinib, finally, it is prepared by coating kidney cancer cell membrane.The pH-responsive nanogel prepared in the present application as the carrier of polypeptide and drug, with the advantages of good biocompatibility, high drug loading rate, etc., in the tumor microenvironment response dissociation release drug, real-time monitoring is carried out to focus area during treatment.

This invention provides a transdermal follicular delivery system for active ingredients based on a protein carrier. The system comprises a composite structure of a small molecule active ingredient, a bioactive protein, and a polymer shell, with a size of 40-150 nm and a polymer shell thickness of 10-70 nm. The zeta potential of this protein carrier-based transdermal follicular delivery system is positively charged. The small molecule active ingredient is loaded within the bioactive protein, and the polymer shell coats the bioactive protein to form a protein nanogel. Both the small molecule active ingredient and the bioactive protein have beneficial effects on hair follicles. When this transdermal follicular delivery system is applied to the surface of a hair follicle or skin, the functional small molecules are delivered transdermally along with the nanogel to the interior of the hair follicle and surrounding tissues, where they are released in a controlled manner, thus effectively exerting their effects.

The present application relates to a kind of glutathione-responsive exosome biomimetic dendrimer nanogel vaccine and its preparation method and application.The vaccine is loaded with 5-Aza, coated with tumor cell Ex and modified R837 in turn to prepare dendrimer nanogel as carrier.The vaccine realizes precise drug delivery of tumor by virtue of the homologous targeting characteristics of Ex, and can target drug release in response to the high glutathione characteristics of tumor microenvironment.5-Aza exerts epigenetic therapy effect through DNA demethylation, while inducing pyroptosis type ICD of tumor cells.R837 immunoadjuvant can synergize with tumor-associated antigens carried by Ex to promote the maturation of dendritic cells, promote the polarization of M2 type tumor-associated macrophages to M1 type, and reverse the tumor immunosuppressive microenvironment.The present application combines epigenetic therapy and immunotherapy, providing a new strategy for the clinical treatment of pancreatic cancer, and has wide clinical application prospect.

The application provides a preparation method of a trehalose nanogel carrier system for delivering recombinant human elastin, and belongs to the technical field of biological medicine and cosmetics. The carrier system is prepared by using trehalose acrylate and acrylamide as monomers, preparing a nanogel core through a reverse microemulsion polymerization method, and sequentially constructing a hyaluronic acid surface modification and a glutathione-responsive recombinant human elastin compound loading. The system has a triple controlled release mechanism: amide catalytic hydrolysis realizes pH response slow release, hyaluronidase enzymatic degradation enhances epidermal permeation, and glutathione triggers disulfide bond rupture to realize intracellular targeted release. The nanogel has an encapsulation efficiency higher than 85%, a 72-hour cumulative release rate of 63% in PBS, and can significantly improve the transdermal absorption rate of recombinant human elastin and the intracellular bioavailability. The carrier system has good stability and high biocompatibility, and can be used for preparing high-efficiency anti-aging skin care products.

The application provides a directional thrombolytic pro-endothelialization composite small-caliber artificial blood vessel and a preparation method thereof. The method activates and chemically modifies the surface of an expanded polytetrafluoroethylene (ePTFE) artificial blood vessel through plasma treatment, introduces active carboxyl groups and covalently grafts a pro-endothelial adhesion polypeptide REDV; then, by using the strong adhesion of a polydopamine (PDA) coating, nano-hydrogel particles loaded with thrombolytic drugs t-PANPs are fixed on the inner surface of the blood vessel. The application actively recruits endothelial cells by the bottom layer of the REDV polypeptide, and at the same time, realizes on-demand and controlled release of t-PA for thrombolysis by the upper layer of the nano-hydrogel, thereby solving the problems of easy thrombosis and intimal hyperplasia of the small-caliber artificial blood vessel. The preparation process condition is mild, the performance is stable, the synergistic effect of antithrombosis and pro-endothelialization is realized, and the application has a good clinical application prospect.

This invention relates to a modulus-controllable gel nanomotor and its preparation method, comprising the following steps: mixing hyaluronic acid solution, a first coupling agent, and cystamine dihydrochloride to undergo a condensation reaction to prepare cystamine-modified hyaluronic acid monodisulfide compound HA-cysta; mixing HA-cysta, solvent, and crosslinking agent uniformly to obtain a pre-crosslinked mixture; adding an oil phase containing a surfactant to the pre-crosslinked mixture to form an emulsion; heating the emulsion to undergo a crosslinking reaction, followed by washing, centrifugation, and purification to obtain hyaluronic acid nanogels; and mixing the hyaluronic acid nanogels, a second coupling agent, and a biological enzyme to react and obtain the gel nanomotor. This invention features simple steps, and the prepared gel nanomotor possesses controllable particle size, elastic modulus, and kinematic properties, which is beneficial for meeting various application requirements.

The application discloses a preparation method and application of a natural active ingredient compound nanogel agent for animals, and belongs to the technical field of veterinary drug preparations. The natural active ingredient compound nanogel agent disclosed by the application is composed of natural active ingredients, a gel matrix and additional agents, is reasonable in formula, simple in process, stable in property, convenient to use and safe and long-acting. The natural active ingredient compound nanogel agent contains two synergistically acting natural active ingredients, can inhibit the proliferation of endometritis pathogenic bacteria such as staphylococcus aureus and streptococcus, and reduce the expression of pro-inflammatory cytokines at an inflammation site; meanwhile, the natural active ingredient compound nanogel agent has a certain slow-release capacity, slowly releases natural active ingredient nanoparticles easy to be taken by cells, can realize long-time residence and high-efficiency absorption of drugs at a drug administration site, and thus can enhance the action time, reduce the drug administration frequency and improve the curative effect.

This invention provides a nanogel dressing, its preparation method, and its application. The nanogel dressing comprises a hydrogel and a nanoparticle carrier loaded within the hydrogel; the nanoparticle carrier comprises a two-dimensional carbon material and gelatin coated on the surface of the two-dimensional carbon material. In this invention, the nanogel dressing is used to deliver wound-healing drugs, improving drug stability, prolonging the treatment time, exhibiting high drug loading capacity, and possessing good biocompatibility and mechanical properties, making it particularly suitable for treating chronic wounds such as those caused by diabetes.

The application discloses a TGase-plant protein peptide nanogel delivery system loaded with a fat-soluble active substance, and belongs to the technical field of nanogels. The TGase-plant protein peptide nanogel delivery system loaded with a fat-soluble active substance is prepared by reasonably utilizing plant protein peptides. In order to solve the problems of poor stability of soybean peptides and possible unsatisfactory embedding effect, TGase is added to the nanogel delivery system, so that the effective embedding of the fat-soluble active substance can be met, and the effective release of the fat-soluble active substance can also be met. Once the nanogel is ingested, the nanogel will be degraded in the gastrointestinal tract, so that the stable release of the active substance is ensured. Meanwhile, the peptides or amino acids generated after digestion also have biological activity, and can play a synergistic role with the bioactive compounds.

The present invention pertains to production of special whey protein nanogels by denaturation of whey protein and furthermore to the resulting whey protein nanogels, and their use in food products, such as beverages. The present whey protein nanogels contribute with an extraordinarily low viscosity contribution when used in high concentration in beverage and liquid products which is a clear indication of their uniqueness.

The application discloses a flexible cobalt ferrite film biosensor and a preparation method thereof, and belongs to the technical field of biosensors. The sensor comprises a flexible cobalt ferrite / PDMS film; the surface of the flexible cobalt ferrite / PDMS film is doped with an AuNPS nano-gold layer; the AuNPS nano-gold layer is modified with an antibody; the flexible cobalt ferrite / PDMS film is obtained by adding cobalt ferrite nanoparticles into A glue of PDMS to obtain cobalt ferrite nanogel fluid, and then mixing the cobalt ferrite nanogel fluid with B glue of the PDMS film; the mass ratio of the cobalt ferrite nanoparticles to the A glue of the PDMS is 1:4-6; the flexible film can exhibit good electromagnetic performance under the combination of the cobalt ferrite nanometallic particles and the reduced gold layer, and can realize detection of various substances based on specific recognition of antigens and antibodies.