Preparation method of hyaluronic acid nanogel

A nano-gel and hyaluronic acid technology, applied in the field of pharmacy, can solve the problems of easy degradation and inactivation, limited therapeutic effect, etc., and achieve the effects of simple preparation technology, high encapsulation capacity, and good drug sustained-release effect.

Inactive Publication Date: 2015-10-07
QINGDAO CENT HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Existing prominent problems are: 1. Basic fibroblast growth factor is easily degraded and inactivated in the oral environment; 2. With th

Method used

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  • Preparation method of hyaluronic acid nanogel
  • Preparation method of hyaluronic acid nanogel

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0031] Take 30 mg of chitosan quaternary ammonium salt with a molecular weight of 40 kDa, dissolve it in 10 mL of deionized water, add 10 mL of 2 mg / mL basic fibroblast growth factor solution dropwise under stirring, and 1 mL of 0.2% (w / v) tripolyphosphoric acid Sodium solution, drop in 20 minutes, stir at 200 rpm at room temperature for 30 minutes, add 10 mL of sodium hyaluronate solution containing 0.3% (w / v) dropwise under stirring conditions, drop in 30 minutes, adjust with sodium hydroxide pH to pH 4.0, stirred at room temperature, stirred at 200 rpm for 5 hours, centrifuged at 15,000 rpm for 2 hours, removed the supernatant, and freeze-dried to obtain the chitosan quaternary ammonium hyaluronic acid loaded with basic fibroblast growth factor acid nanogel.

Embodiment 2

[0033]Take 30 mg of chitosan quaternary ammonium salt with a molecular weight of 90 kDa, dissolve it in 10 mL of deionized water, add 10 mL of 2 mg / mL basic fibroblast growth factor solution dropwise under stirring, and 1 mL of 0.3% (w / v) tripolyphosphoric acid Sodium solution, drop in 20 minutes, stir at 200 rpm at room temperature for 30 minutes, add 10 mL of sodium hyaluronate solution containing 0.4% (w / v) dropwise under stirring conditions, drop in 30 minutes, adjust with sodium hydroxide pH to pH 5.0, stirred at room temperature, stirred at 200 rpm for 5 hours, centrifuged at 15,000 rpm for 2 hours, removed the supernatant, and freeze-dried to obtain the chitosan quaternary ammonium hyaluronic acid loaded with basic fibroblast growth factor acid nanogel.

Embodiment 3

[0035] Take 30 mg of chitosan quaternary ammonium salt with a molecular weight of 150 kDa, dissolve it in 10 mL of deionized water, add 10 mL of 2 mg / mL basic fibroblast growth factor solution dropwise under stirring, and 1 mL of 0.5% (w / v) tripolyphosphoric acid Sodium solution, drop in 20 minutes, stir at 200 rpm at room temperature for 30 minutes, add 10 mL of sodium hyaluronate solution containing 0.5% (w / v) dropwise under stirring conditions, drop in 30 minutes, adjust with sodium hydroxide pH to pH 6.0, stirred at room temperature, stirred at 200 rpm for 5 hours, centrifuged at 15000 rpm for 2 hours, removed the supernatant, and freeze-dried to obtain the chitosan quaternary ammonium hyaluronic acid loaded with basic fibroblast growth factor acid nanogel.

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Abstract

The invention discloses a preparation method of hyaluronic acid nanogel. The target product hyaluronic acid nanogel is synthesized from chitosan quaternary ammonium salt and sodium hyaluronate through an ion crosslinking and polymer coacervation two-step technology, the average particle size of the product is 150-230nm, and the Zeta potential of the product is -28.6 ~ -36.7mV. The hyaluronic acid nanogel prepared in the invention can be used for treating oral ulcer, can maintain the effective active concentration of a basic fibroblast growth factor in the oral ulcer position for a long time, and improves the clinic treatment effect.

Description

technical field [0001] The invention belongs to the field of pharmacy and relates to a preparation technology of nano gel sustained-release particles, in particular to a preparation method of hyaluronic acid nano gel. Background technique [0002] Oral ulcer is a common disease in daily life, mostly occurs on the tip of the tongue, belly of the tongue, and buccal mucosa, accompanied by intermittent, tactile tingling, which seriously affects the quality of life of patients. At present, there is still no effective method for the treatment of oral ulcers. The traditional treatment method focuses on local pain relief, but does not pay enough attention to how to speed up the healing of the ulcer surface. [0003] Basic fibroblast growth factor is a multifunctional protein bioactive substance, which has a strong repair effect on wound surface and nerve injury. The use of basic fibroblast growth factor to treat oral ulcers has been reported. Existing prominent problems are: 1. Ba...

Claims

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Application Information

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IPC IPC(8): A61K9/06A61K38/18A61K47/36A61P1/02
Inventor 徐全臣王志国许晓燕袁昌青吉秋霞蔡秀磊杨甫进
Owner QINGDAO CENT HOSPITAL
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