43results about How to "High magnetic content" patented technology

The invention belongs to the technical field of bioseparation material, in particular to a magnetic composite microsphere with nucleocapsid structure and a preparation method thereof. Nano ferroferric oxide decorated by oleic acid is firstly used as raw material, and is polymerized through template fine emulsion so as to prepare the monodisperse magnetic composite microsphere with high magnet content; and then the monodisperse micosphere is polymerized through seed emulsion and is added with different amount of shell monomer to prepare the magnetic composite microsphere with the nucleocapsid structure and the magnet content of 20wt percent to 80wt percent. The grain size of nucleocapsid composite microsphere is in narrow distribution, and the magnet content of the microsphere is controllable and the surface has functional reactive group; therefore, the microsphere of the invention can be surface-modified into a magnetic carrier of biological active molecule by chemical crosslinking to be applied in the biomedicine field. The method of the invention can be simply operated and the process of which can be controlled, thus having industrialized prospect.

The invention discloses a method for preparing silicon dioxide-coated magnetic microspheres, which belongs to the technical field of nano-magnetic materials. In the method, the characteristic that nanoparticles are easily arranged on a two-phase solution interface is utilized to form oil-in-water emulsion or microemulsion which is stabilized by oil-soluble magnetic nanoparticles and the hydrolysis of tetraethyl orthosilicate is further produced on the surfaces of droplets, so that a silicon dioxide-coated layer is formed. The method has the advantages of no need of using any surfactant, simple reaction operation process and short reaction time; particle diameters of the coated magnetic microspheres are 50 to 400nm; and the magnetic content of the microspheres can be simply controlled at the same time when the particle diameters of the magnetic microspheres are controlled.

The invention specifically relates to surface carboxyl-functionalized core-shell type magnetic composite microballoons and a preparation method thereof, belonging to the technical field of functional materials. According to the invention, the core of the core-shell type magnetic composite microballoons is a magnetic ferriferrous oxide nanoparticle cluster, and the shell of the microballoons is a cross-linked carboxyl-containing polymer network. The preparation method comprises the following steps: at first, preparing the citric acid stabilized magnetic nanoparticle cluster (thereafter referred as magnetic cluster for short); then, modifying the surface of the magnetic cluster with active vinyl function groups by using the sol-gel method; finally, preparing the monodisperse core-shell type magnetic polymer composite microballoons which have high magnetic responsibility and contain abundant active carboxyl groups on their surface through distillation-precipitation polymerization. A variety of bioactive molecules can be bonded with the surface of the core-shell type microballoons through chemical modification for application in the field of biomedicine. Furthermore, the method can accurately control the thickness and cross-linking degree of the shell of polymers. The invention has the advantages of simple operation and controllable process and has a good application prospect.

The invention relates to a preparation method of superparamagnetic composite microballoons used in biomedicine. First, a coprecipitation method is used to prepare superparamagnetic Fe3O4 nanoparticles, and after modified by a surfactant, the prepared Fe3O4 nanoparticles are dispersed into deionized water to form a water-based magnetic liquid. Second, inorganic/organic core shell microballoons with carboxyl function groups on surfaces are prepared. The superparamagnetic composite microballoons are of an inorganic/organic core shell structure, have composite material characteristics and biological effects. The microballoons have active function groups on the microballoon surfaces, and therefore can be combined with a plurality of biologically-active substances. The magnetic microballoons prepared by the method of the invention have active carboxylic groups on the microballoon surfaces, and have superparamagnetism and a large magnetic content, and therefore the microballoons are subject to magnetization separation under an externally applied magnetic field and can lose magnetism immediately after the magnetic filed is removed. The magnetic microballoons prepared in the invention can be widely applied in the detection and separation operation of tumor cells in the field of biomedicine. The method has simple experimentation, is rapid, and has low cost.

The invention provides a preparation method and an application of a core-shell magnetic composite microsphere capable of separating glycoprotein. The preparation method is characterized in that a core of the core-shell magnetic composite microsphere is a magnetic iron oxide black nanoparticle cluster and a shell is a crosslinked polymer network rich in carboxyl, then carboxyl and aminobenzene boron ester carry out amidation to carry out surface modification, and glycoprotein can be quickly separated and enriched under the physiological conditions via plenty of surface immobilized boron esters. The preparation method comprises the following steps of: firstly preparing a magnetic nanoparticle cluster with stable sodium citrate, secondly adopting a sol-gel method to modify the surface of the magnetic cluster with active vinyl functional groups, thirdly preparing high-magnetic-responsiveness monodispersed core-shell magnetic polymer composite microsphere rich in carboxyl on the surface via reflux precipitation polymerization, fourthly carrying out amidation on aminobenzene boron ester and carboxyl to modify the surface of the core-shell magnetic composite microsphere with plenty of boron ester groups and finally carrying out separation and enrichment on glycoprotein. The method provided by the invention is simple, is controllable in process, has higher glycoprotein separation and purification efficiency and can carry out separation and enrichment under the physiological conditions.

The invention discloses a preparation method of an epoxy functional core-shell structure magnetic polymer microsphere. The core of the core-shell structure magnetic polymer microsphere disclosed by the invention is ferroferric oxide nanometer crystal druse; the shell layer of the core-shell structure magnetic polymer microsphere is cross-linked polymer containing epoxy functional group. The preparation method comprises the following three steps of: (1), preparing the ferroferric oxide nanometer crystal druse with a high value of saturated magnetic by adopting a solvothermal method; (2), modifying the surface of the ferroferric oxide nanometer crystal druse into a dual-bond functional group by adopting a sol-gel method; (3), coating the polymer shell layer with the epoxy functional group on the surface of the ferroferric oxide nanometer crystal druse by adopting a distillation-precipitation polymerization method to obtain the epoxy functional core-shell structure magnetic polymer microsphere. The epoxy functional core-shell structure magnetic polymer microsphere is narrowly distributed in grain size, controllable in magnetic content, high in surface epoxy radial reaction activity, easy to modify the functional groups including ammonia group, hydroxyl, group, the mercapto group and the like on the surface, capable of being applied to the industrial waste water treatment field by efficiently adsorbing a plurality of heavy meals or organic pollutants, and good in application prospect.

The invention discloses a magnetic nano-sphere grafted with an arginine polymer brush, and a preparation method and an application thereof. The magnetic nano-sphere grafted with the arginine polymer brush is formed by a nano-Fe3O4 magnetic sphere, a polydopamine polymer middle layer which coats the surface of the Fe3O4 magnetic sphere and the arginine polymer brush grafted to the polydopamine polymer layer; and the arginine polymer brush is composed of a PAMA polymer brush introduced to the polydopamine polymer middle layer through polymerization and arginine grafted to the PAMA polymer brush.The magnetic nano-sphere grafted with the arginine polymer brush, adopting the Fe3O4 magnetic sphere as a core, has an excellent magnetic response performance; the polydopamine polymer middle layer having a controllable thickness is sequentially introduced to the surface of the Fe3O4 magnetic sphere, a middle layer of macromolecules with controllable thickness and the PAMA polymer brush grafted with arginine are sequentially introduced, and the magnetic nano-sphere has a uniform particle size and a narrow particle size distribution, and can well respond to an externally applied magnetic field, so the magnetic nano-sphere grafted with the arginine polymer brush facilitates the enrichment and separation of phosphorylated polypeptides, and has a great significance to study the physiologicalbehavior protein phosphorylation process.

The invention discloses a cassava starch magnetic microsphere and a preparation method thereof. The preparation method comprises the following steps: performing cross-linking reaction of a mixed system of magnetic fluid and polyethylene glycol with cassava starch and glutaraldehyde under the action of ammonium persulfate; and the emulsifying agent is not added during preparation. The preparation method of the cassava starch magnetic microsphere is simple, the starch is not required to be gelatinized in advance, no emulsifying agent is added, the reaction condition is mild, the post-processing is simple and convenient, and the magnetic content of the obtained cassava starch magnetic microsphere is high.

The invention discloses a magnetic liposome and a method for preparing the same. The magnetic liposome wraps medicine inside the liposome; and magnetic particles are coated outside the liposome. The preparation method comprises the following steps that: a yolk lecithin solution and a cholesterin solution are mixed in an eggplant-shaped bottle according to the molar ratio of 2:1 to 2.5:1; the mixture is subjected to rotary evaporation to form a liposome film; then a phosphate buffering solution is used to carry out full hydration on the liposome film to obtain the liposome of which the surface is provided with negative charges; then, cationic polyelectrolyte-poly diallyl dimethyl ammonium chloride, anionic polyelectrolyte which is sodium polystyrene sulfonate and cationic polyelectrolyte which is poly diallyl dimethyl ammonium chloride are subjected to alternate adsorption; and finally, through the charge action and a self-assembling method, Fe3O4 magnetic particles of which the surfaces are provided with negative charges are deposited on the surface of the liposome to obtain the magnetic liposome. As the inside of the liposome is packed with the medicine, the magnetic particles are deposited on the surface of the liposome and a finite space of the inner space of the liposome is totally packed with the medicine, the packing amount of the medicine is improved and the magnetic content is simultaneously improved.

The invention discloses monodisperse strong-magnetism immunologic nanoparticles. The particle size of the monodisperse strong-magnetism immunologic nanoparticles ranges from 5 nm to 5 microns. The invention further discloses a preparation method of the monodisperse strong-magnetism immunologic nanoparticles. The method includes the following steps that firstly, ferroferric oxide nanoparticles are prepared; secondly, monodisperse silicon hydroxyl magnetic nanoparticles are prepared; thirdly, monodisperse amination magnetic nanoparticles are prepared; fourthly, the monodisperse strong-magnetism immunologic nanoparticles are prepared. The monodisperse strong-magnetism immunologic nanoparticles and the preparation method thereof have the advantages that superparamagnetism is good, the magnetic content is high, dispersity is good, the agglomeration phenomenon dose not exist, the appearance is regular and standard, the particle size is unified and controllable, and functional modification is easy.