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Magnetic nano-sphere grafted with arginine polymer brush, and preparation method and application thereof

A technology of magnetic nanospheres and polymer brushes, which is applied in the preparation methods of peptides, alkali metal compounds, chemical instruments and methods, etc., can solve the problems of high anaerobic requirements, unfavorable popularization and application, complicated preparation process, etc.

Active Publication Date: 2018-06-19
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this preparation method can prepare magnetic composite materials with magnetic separation performance and high enrichment efficiency for phosphorylated polypeptides, its existence requires high anaerobic requirements, which makes the preparation process relatively complicated; ATRP polymerization method, using a low-valence metal salt as a catalyst is not only easy to be oxidized but also difficult to preserve, and the amount of catalyst used is large, and it pollutes the environment, which does not conform to the direction of green development; therefore, it is not conducive to the above-mentioned method for preparing magnetic composite materials. Promotion and application in the field

Method used

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  • Magnetic nano-sphere grafted with arginine polymer brush, and preparation method and application thereof
  • Magnetic nano-sphere grafted with arginine polymer brush, and preparation method and application thereof
  • Magnetic nano-sphere grafted with arginine polymer brush, and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1- Embodiment 5

[0047] Embodiment 1-embodiment 5 prepares Fe 3 o 4 / Polymer nanosphere

[0048] Take the raw material according to table 1, and prepare Fe according to the following method in conjunction with the process parameters given in table 1 3 o 4 / Polymer nanospheres: Add Fe to Tris buffer containing dopamine at pH 8.2 to 8.5 3 o 4Magnetic balls, and then stirred and reacted for 2h to 12h under the condition of the opening of the reaction vessel to obtain the first reaction solution, magnetically separated the first reaction solution and collected the separated solid product, and then washed the solid product with deionized water to remove untreated Coated in Fe 3 o 4 The polydopamine layer on the surface of the magnetic ball and the unreacted dopamine raw materials are obtained by the polydopamine layer-coated Fe 3 o 4 Magnetic ball, namely Fe 3 o 4 / Polymer nanosphere; pH 8.2-8.5 buffer solution containing dopamine is formed by dissolving dopamine in Tris buffer solution, ...

Embodiment 6- Embodiment 9

[0052] Embodiment 6-embodiment 9 prepares Fe 3 o 4 / Polymer-Br nanospheres

[0053] Take the raw material according to table 2, and prepare Fe according to the following method in conjunction with the process parameters given in table 2 3 o 4 / Polymer-Br nanosphere: the Fe that embodiment 3 obtains 3 o 4 / Polymer nanospheres are uniformly dispersed in DMF to obtain Fe 3 o 4 / Polymer nanosphere suspension; first vacuumize the reaction container of the two-necked flask with a stirrer for 5 minutes to 10 minutes, then pass nitrogen to normal pressure, and repeat this operation three times to ensure that the oxygen in the reaction container is completely removed. state; then add Fe 3 o 4 / Polymer nanosphere suspension, in the state of magnetic stirring, to Fe 3 o 4 Add 0.5mL-2mL triethylamine dropwise to the / Polymer nanosphere suspension to obtain the first mixed solution, then add 0.4mL-1.8mL 2-bromoisobutyryl bromide dropwise to the first mixed solution under ice-bath...

Embodiment 10- Embodiment 13

[0056] Embodiment 10-embodiment 13 prepares Fe 3 o 4 / Polymer / PAMA nanospheres

[0057] Take the raw material according to table 3, and prepare Fe according to the following method in conjunction with the process parameters given in table 3 3 o 4 / Polymer / PAMA nanospheres: the Fe prepared in Example 9 3 o 4 / Polymer-Br nanospheres are uniformly dispersed in DMF to obtain Fe 3 o 4 / Polymer-Br nanosphere suspension; first vacuumize the reaction container of the two-necked flask with a stirrer for 5 minutes to 10 minutes, then pass nitrogen to normal pressure, and repeat this operation three times to ensure that the oxygen in the reaction container is completely removed; under nitrogen environment , add Fe to the reaction vessel 3 o 4 / Polymer nanosphere suspension, in the state of magnetic stirring, to Fe 3 o 4 Add the DMF solution of the catalyst and the complex and the DMF solution of the monomer AMA to the Polymer-Br nanosphere suspension in turn to obtain the secon...

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Abstract

The invention discloses a magnetic nano-sphere grafted with an arginine polymer brush, and a preparation method and an application thereof. The magnetic nano-sphere grafted with the arginine polymer brush is formed by a nano-Fe3O4 magnetic sphere, a polydopamine polymer middle layer which coats the surface of the Fe3O4 magnetic sphere and the arginine polymer brush grafted to the polydopamine polymer layer; and the arginine polymer brush is composed of a PAMA polymer brush introduced to the polydopamine polymer middle layer through polymerization and arginine grafted to the PAMA polymer brush.The magnetic nano-sphere grafted with the arginine polymer brush, adopting the Fe3O4 magnetic sphere as a core, has an excellent magnetic response performance; the polydopamine polymer middle layer having a controllable thickness is sequentially introduced to the surface of the Fe3O4 magnetic sphere, a middle layer of macromolecules with controllable thickness and the PAMA polymer brush grafted with arginine are sequentially introduced, and the magnetic nano-sphere has a uniform particle size and a narrow particle size distribution, and can well respond to an externally applied magnetic field, so the magnetic nano-sphere grafted with the arginine polymer brush facilitates the enrichment and separation of phosphorylated polypeptides, and has a great significance to study the physiologicalbehavior protein phosphorylation process.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and relates to a magnetic nanosphere grafted with a polymer brush enriched in phosphorylated polypeptide, a preparation method and an application thereof. Background technique [0002] The guanidino group shows a good affinity for phosphorylated polypeptides. In view of this, a series of new materials with guanidino groups have been prepared. For example, the document Guanidyl-functionalized graphene as a bifunctional adsorbent for selective enrichment of phosphopeptides (Chem.Commun., 2014, 50, 10963-10966) discloses a bifunctional adsorbent for enriching phosphorylated polypeptides, which uses graphene as the base material , and then use the -COOH group on the graphene to introduce the -COCL group, and then introduce the -NH 2 , and finally the guanidine group was introduced through a condensation reaction. The guanidinium group on the surface of this material is a monomolecular layer, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/28B01J20/30C07K1/22
CPCB01J20/265B01J20/28004B01J20/28009B01J20/28019C07K1/22
Inventor 蓝芳罗斌吴尧
Owner SICHUAN UNIV
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