129 results about "Magnetic nanospheres" patented technology

The invention discloses a method for preparing carbon-coated superparamagnetic ferroferric oxide gel, which comprises: dissolving ferrocene and surfactant in an acetone solvent; adding solution of hydrogen peroxide into the solution to directly oxidize the ferrocene to synthesize polycrystal uniform magnetic nano balls under a low-temperature (180 to 240 DEG C) solvothermal condition; and allowing the polycrystal uniform magnetic nano balls to react in a magnetic field to prepare a magnetic linear nano material. The polycrystal uniform magnetic nano balls and nano chains are characterized in that: the particle size of the polycrystal uniform magnetic nano balls is limited to a narrow range and controllable; and the nano chains consist of uniform spherical particles and have high stability, and the inter-chain distance is controllable. The superparamagnetic polycrystal uniform nano ball and linear nano chain materials have high superparamagnetism and high chemical stability and gel stability and have promising application prospect in the field of biomedicine, nano self-assembly and the like; the nano balls are grafted by medicaments and can be used as medicament carriers; under the action of the magnetic field, the nano balls can be used as magnetic control photonic crystal; and the nano chains have a promising application prospect in the field of Bragg reflectors, magnetic probes, biomedicine and the like.

The invention provides metal-organic framework materials with magnetic cores and preparation and application thereof, and belongs to the technical field of magnetic metal-organic framework materials. FeCl3 6H2O is adopted for preparing superparamagnetic Fe3O4 cores, cetyl trimethyl ammonium bromide is used as a template agent, sodium silicate is used as silicon sources of SiO2 shell layers, mesoporous SiO2 shell layers are formed on the surfaces of the Fe3O4 cores by adopting an ultrasonic wave method, the template agent is removed by roasting under protection of N2 after drying, and magnetic SiO2@Fe3O4 nanospheres are prepared. The SiO2@Fe3O4 nanospheres are used as cores, an in-situ self-assembly mesh structure IRMOFs is carried out on the surfaces of the SiO2@Fe3O4 cores through Zn metal ions and organic carboxylic acid ligands by adopting the ultrasonic wave method. The novel MOFs materials can be used as catalysts of liquid phase catalysis reaction, and also have application values in the aspects of adsorption, separation, biological medicine and the like.

The invention discloses a thiohydroxy-modified magnetic MOFs adsorbent and a preparation method and application thereof. The preparation method comprises the following steps: (1) preparing magnetic Fe3O4 nanospheres with the particle size of 10-20 nm by adopting a coprecipitation method; (2) preparing Fe3O4@SiO2 with a core-shell structure by adopting a Stober silicone coupling method; (3) preparing Fe3O4@SiO2@MOF through a solvothermal in-situ growth method; (4) carrying out thiol-functionalization on Fe3O4@SiO2@MOF synthesized in the step (3) in a solvent auxiliary ligand exchange manner, and preparing the thiol-functionalized magnetic MOFs adsorbent with different proportions through adjusting the proportion of mercaptoacetic acid to Fe3O4@SiO2@MOF. According to the invention, the preparation method is mild in reaction conditions, low in cost, and simple and convenient to operate; the prepared magnetic MOFs adsorbent has the advantages of being high in adsorption speed, large in adsorption capacity and good in selectivity when removing the metal ions in water.

The invention discloses a double-ion response type SERS (Surface Enhanced Raman Scattering) probe and a preparation method thereof. The probe comprises two nano particles with independent core-shell structures, wherein a carrier nano particle comprises four layers of core-shell structures; the four layers of core-shell structures are as follows: the innermost layer is made of Fe3O4 magnetic nano balls, a layer of silicon dioxide is coated outside the innermost layer, the second-outer layer is a gold foil shell layer, and the outermost layer is an oligonucleotide coating layer; an object nano particle comprises two layers of core-shell structure; the two layers of core-shell structure are as follows: the inner core is made of Raman molecule marked gold nano-balls, and a shell is an oligonucleotide coating layer. Due to the adoption of a specific base sequence in the oligonucleotide coating layer, the identify and SERS trace detection on two metal ions, namely silver/mercury, in an environment solution can be performed at the same time; furthermore, under the action of an external magnetic field, the probe and the obtained silver/mercury ions can be rapidly separated from an initial environment solution system, and therefore, the function of purifying the environment is realized.

The invention relates to a magnetic graphene adsorbing material for adsorbing phosphorus in water, a preparation method and an adsorption method. According to the preparation method, firstly, spherical Fe3O4 magnetic nanospheres are supported on the surface of GO with a coprecipitation method under the ultrasonic action, and Fe3O4-supported GO (Fe3O4-GO) is prepared; Fe3O4-GO is reduced by hydrazine hydrate, and Fe3O4-supported graphene(Fe3O4-GNS) is prepared; Fe3O4 is coated with active-amino-terminated PZD with a copolymerizing precipitation method, and PZD-coated Fe3O4-supported graphene (PZD@Fe3O4-GNS) is prepared; a layer of La2O3 nanoparticles are deposited on the surface of PZD@Fe3O4-GNS, and La2O3-PZD@Fe3O4-GNS quaternary nanoparticles are prepared. The preparation conditions are mild, the operation is simple, a product is simple to separate, and the obtained product has excellent adsorption performance on phosphate particles, is free of pollution on the ecological environment and has quite broad application prospect in the aspects of efficient and rapid treatment of a large water area with phosphorus pollution and resource recycling.

The invention discloses a composite nano-drug for integrated tumor diagnosis and treatment and a preparation method thereof. A chemotherapeutic drug, a photodynamic drug and shRNA plasmids capable of reversing multidrug-resistant MDR1 genes of tumor cells are located through a nano-carrier, a magnetic nano-sphere with precious metal nano-particles arranged on the surface serves as a kernel of the nano-carrier, mesoporous silica with the surface modified with amino groups serves as a shell of the nano-carrier, and a polyelectrolyte thin film layer structure with pH response is formed on the surface of the kernel-shell structure through a layer-by-layer self-assembly technology. The composite nano-drug integrates targeted drug delivery, multimode imaging, chemotherapy, photodynamic therapy and gene therapy, can be applied to early diagnosis of tumors, image mediation and real-time monitoring treatment at the same time and overcomes the defect that tumor diagnosis and treatment are separate in the prior art; besides, the composite nano-drug is simple in preparation process, high in operability and suitable for industrial production.

The invention relates to a method for detecting bacteria. The method comprises the steps as follows: coupling magnetic nanospheres and fluorescent nanospheres with mouse typhus salmonella antibodies so as to obtain mouse typhus salmonella-targeted immunologic magnetic spheres and immunologic fluorescent spheres, and adding the mouse typhus salmonella-targeted immunologic magnetic spheres and immunologic fluorescent spheres into a detection system so as to realize magnetic capture and fluorescent labeling to the mouse typhus salmonella simultaneously. About 10 CFU/mL of the mouse typhus salmonella can be detected through observation of a fluorescent microscope; and a good quantitative relation is obtained through detection of a fluorescence spectrophotometer, the linear range is 105-107 CFU/mL, and R2 is equal to 0.9994. The whole detection process is very simple, high in sensitivity and strong in specificity, and can be finished detection within 1.5 h.

The invention relates to a magnetic nanosphere with a mesoporous shell and a quick preparation method thereof, which belongs to the technical field of magnetic nanospheres. A Fe3O4 core with superparamagnetism is prepared from FeCl3.6H2O, then a mesoporous SiO2 shell is quickly formed on the surface of the magnetic Fe3O4 core with an ultrasonic wave method by taking hexadecyl trimethyl ammonium bromide as a template agent and taking low-cost sodium silicate as a silicon source for wrapping the SiO2 shell on the surface of the magnetic Fe3O4, and finally, the template agent is removed by roasting under the protection of N2, thus obtaining the magnetic SiO2@Fe3O4 nanosphere with the mesoporous SiO2 shell and large specific surface area. The magnetic nanosphere has good application prospects in the aspects of magnetic adsorption, precious metal recovery, target administration carriers, bioprotein separation and the like.

The invention belongs to the technical field of composite material preparation and food safety detection, and particularly relates to a preparation method and application of a magnetic MOF-based bisphenol A molecularly imprinted high-selectivity nano composite material. The preparation method is realized through the following steps: (1) preparing magnetic Fe3O4 nanospheres; (2) preparing a magnetic MOF material Fe3O4 @ ZIF-8; and (3) preparing a magnetic MOF-based bisphenol A molecularly imprinted polymer. The material preparation method is simple and environment-friendly in synthesis and hasthe characteristics of high selectivity, high-efficiency adsorption separation, reusability and the like on a target object. The prepared material does not need pretreatment equipment and can be usedfor enriching and separating pollutant bisphenol A in a water environment and can also be combined with a conventional instrument to realize high-selectivity and high-sensitivity analysis and detection of bisphenol A in the environment or food and the detection sensitivity of bisphenol A is improved. The material can be repeatedly used so that the detection cost is effectively reduced.

Disclosed is a process of preparing magnetite nanoparticles, comprising the following steps: 1) preparing a ferric salt mixed system, wherein a soluble ferric salt is dissolved in glycol at ambient temperature, and then urea and polyethylene glycol are added and mixed homogeneously to obtain the trivalent iron salt mixed system, the mass ratio of glycol to the trivalent iron salt being 15:1 to 60:1, glycol to urea being 20:1 to 100:1, and glycol to polyethylene glycol being 20:1 to 100:1; 2) reacting, wherein the trivalent iron salt mixed system is transferred into a reaction autoclave, sealed and placed into a heating device to react at a temperature of 200 to 300° C. for 8 to 72 hours; and 3) washing, wherein after the reaction system is naturally cooled down to ambient temperature, the product is taken out, and washed with anhydrous ethanol and water in turn to obtain the magnetite nanoparticles. The soluble iron salt includes ferric chloride, ferric sulfate, ferric acetate and ferric nitrate. The obtained nanospheres exhibit a uniform distribution of the particle diameter with a good dispersity in water. The nanospheres have superparamagnetism, and their particle diameter can be controlled by varying the reaction time as desired.

The invention relates to a method for identifying circulating tumor cells.The method includes the steps that antibodies for specifically identifying the tumor cells on a magnetic nanoparticle coupling are utilized to modify antibodies for specifically identifying the tumor cells and white blood cells respectively through two fluorescent nanospheres of different colors so as to obtain three nanospheres having immune functions; meanwhile the three nanospheres having the immune functions are added to a whole blood system to be detected, and synchronous capture and marking of the circulating tumor cells are achieved, enriched cells are directly observed under a fluorescence microscope through simple magnetic separation to achieve the identification purpose.The method is simple in operation, the loss of the circulating tumor cells is small, the nanospheres having the immune functions almost have no influence on the activities of target cells, and 93% of the tumor cells remain good biological activities after being identified and can be cultured in vitro.The method is an effective method for prognosis, recurrence monitoring and individualized therapies of clinical cancer patients.

The invention relates to a preparation method for magnetic multiple-template steroid estrogen molecular imprinting nanospheres. The method includes synthesizing magnetic nanospheres having a particle size of about 200 nm through a hydrothermal method; coating a silicon coating on the magnetic nanospheres through a sol-gel method; adding functional monomers, three steroid estrogens into ethanol to obtain a multiple templates/functional monomer composite; adding silicon-coated magnetic nanospheres and a catalyst into the multiple templates/functional monomer composite to perform polymerization; separating a solid polymer through an additional magnetic field; and eluting the solid polymer and drying to obtain the magnetic multiple-template steroid estrogen molecular imprinting nanospheres. According to the preparation method, specific identification of molecular imprinting and easy separability of the magnetic nanospheres are combined. The preparation method is simple and conditions are mild. The polymer prepared is uniform in particle size, strong in stability, and good in water solubility, and can achieve rapid high-selectivity removal and enrichment of the three steroid estrogens in trace amount in the environment and drinking water samples.

The invention discloses an amino hyperbranched chlorogenic acid molecularly-imprinted magnetic nanosphere as well as a preparation method and application thereof. The preparation method comprises the following steps: first synthesizing an amino magnetic nanosphere; then adding the amino magnetic nanosphere and methyl acrylate into ethanol, stirring at room temperature, adding ethanediamine, heating, and stirring, so as to obtain an amino hyperbranched modified magnetic nanosphere; adding the amino hyperbranched modified magnetic nanosphere and chlorogenic acid into ethanol, and adding dopamine hydrochloride so as to generate polymerization reaction; and separating out solid polymer generated in the reaction by virtue of an applied magnetic field, and carrying out elution and drying, so as to obtain a target product. By carrying out amino hyperbranching on a carrier, namely magnetic nanosphere, the number of amino active groups on the surface of the magnetic nanosphere is increased, and dopamine is taken as a functional monomer and a cross-linking agent, so that the preparation method is simple and mild in conditions, the prepared target product is uniform in particle size, strong in stability and good in water solubility, and high selective removal and enrichment of chlorogenic acid in a complex matrix can be rapidly realized.

A preparation method of temperature sensitive magnetic western-blotting nanospheres comprises the following steps: synthesizing magnetic nanospheres with a particle size of 200 nm by a hydrothermal method; coating a silicon layer with a thickness of 20 nm on the surfaces of the magnetic nanospheres by a gel-sol method; adding the silicon-coated magnetic nanospheres, functional monomers, template molecules, and a cross-linking agent into a Tris-HCl buffer to obtain a reaction solution; performing polymerization of the reaction solution at a certain temperature to obtain polymers; eluting the polymers by an eluate to obtain the temperature sensitive magnetic western-blotting nanospheres. The advantages of the invention are that: the temperature sensitive magnetic western-blotting nanospheres combine the specific recognition of western blotting, the rapid separation performance of magnetic nanospheres in an external magnetic field, and the temperature responsibility of temperature sensitive materials; the preparation method is simple; the conditions are mild; the price is low; and a new feasible method is provided for the selective removal and enrichment of high-abundance protein components in a complex biological system.

The invention belongs to the technical field of preparation of detection materials, and relates to a preparation method of a magnetic ferroferric oxide nanosphere-based novel pH-responsive imprinted material. The preparation method comprises the following steps: firstly preparing ferroferric oxide nanoparticles and synthesizing mesoporous silicon dioxide coated ferroferric oxide nanospheres, and then performing chemical double bond modification on the mesoporous silicon dioxide coated ferroferric oxide nanospheres; finally, preparing the magnetic nanosphere-based pH-responsive imprinted material. The pH-responsive imprinted material prepared according to the preparation method provided by the invention can solve the problem that the surface of a base material is difficult to be directly coated with a molecularly imprinted polymer layer; the imprinted material adopts a significant core-shell structure consistent with the purpose of the invention; in addition, through combination of theperformance of the molecularly imprinted material and a pH-responsive intelligent material, the pH-responsive imprinted material is successfully applied to efficient detection of sulfamethoxazole.

The invention discloses a preparation method of cordycepin controlled-release magnetic nanospheres, and belongs to the field of medicine-carrying controlled-release magnetic-targeting nanospheres. The cordycepin controlled-release magnetic nanospheres can delay the metabolic time of cordycepin in a body and has a magnetic targeting function. The preparation process comprises the following steps: dissolving Fe3O4 magnetic nanoparticles and sodium alginate in distilled water, dropwise adding a magnetic fluid mixed solution formed by mixing under ultrasonic and high-speed stirring conditions into an oil phase to form a microemulsion system, dropwise adding a chitosan, cordycepin and calcium chloride mixed solution with certain concentration into a microemulsion, reacting for a certain time, performing washing, magnetic separation and filtering to obtain magnetic calcium alginate-chitosan nanospheres, pouring the nanospheres into a water solution of quercetin or genistein, vibrating for absorption equilibrium, filtering, pouring into a chitosan solution, finishing the reaction, and finally performing washing, filtering and freeze-drying to obtain the cordycepin controlled-release magnetic nanospheres.

The invention discloses a preparation method of carbon coated magnetic nano-spherical iron sulfide (Fe7S8) powder; ferrocene and derivatives of the ferrocene are further reacted with ammonium persulfate in one step to prepare carbon coated nano-spherical iron sulfide Fe7S8 powder. The magnetic nano Fe7S8 particle prepared by the method is spherical, the diameter is intensively distributed between 10 nm to 50nm, a coated carbon shell has a graphitizing carbon structure, and the thickness is intensively distributed between 5 nm to 20nm. The preparation method provided by the invention has the characteristics of simple equipment, easy operation, low cost, high efficiency, environmental protection and the like, and is suitable for industrial production; the prepared carbon coated magnetic nano-spherical Fe7S8 powder is good in stability, and the method has wide application prospects in the fields of environmental depollution, drug targeting, absorbing materials and the like.

The invention provides a preparation method of a porous magnetic nanosphere and a method for loading fluorescent quantum dots. The method comprises the following steps of: adding magnetic Fe3O4 nano particles into an alcohol solution of ethylcellulose to obtain an ethylcellulose nanosphere embedded with magnetic particles by utilizing an ultrasonic atomizing and drying technology, and then regulating and controlling the quantity and the size of pores in the nanosphere by regulating the quantity of the Fe3O4 nano particles. Due to the existence of multiple pores, the nanosphere has a large specific surface area and can conveniently combine with a considerable quantity of quantum dots through static adsorption. The porous magnetic nanosphere obtained in the invention and the porous magneticnanosphere adsorbing the quantum dots have application prospects in biomedicine.

The invention provides a magnetic organic solid base catalyst. Magnetic nanospheres with a uniform particle size are prepared by miniemulsion polymerization, and an organic base is supported on the microspheres to obtain the novel magnetic organic solid base catalyst. The method provided by the invention avoids the problem of loss of active sites of a conventional supported catalyst, maintains high efficiency of the catalyst, and effectively prolongs service life of the catalyst; at the same time, separation of the catalyst and a reaction system can be realized under an external magnetic field, and a separation step of the catalyst is greatly simplified; the magnetic organic solid base catalyst can be applied to a transesterification, esterification or aldol condensation reaction; and thepreparation method provided by the invention is simple, and the prepared catalyst has the characteristics of having high efficiency, saving energy, and being non-toxic, easy to separate and recyclable.

The invention discloses a magnetic nano-sphere grafted with an arginine polymer brush, and a preparation method and an application thereof. The magnetic nano-sphere grafted with the arginine polymer brush is formed by a nano-Fe3O4 magnetic sphere, a polydopamine polymer middle layer which coats the surface of the Fe3O4 magnetic sphere and the arginine polymer brush grafted to the polydopamine polymer layer; and the arginine polymer brush is composed of a PAMA polymer brush introduced to the polydopamine polymer middle layer through polymerization and arginine grafted to the PAMA polymer brush.The magnetic nano-sphere grafted with the arginine polymer brush, adopting the Fe3O4 magnetic sphere as a core, has an excellent magnetic response performance; the polydopamine polymer middle layer having a controllable thickness is sequentially introduced to the surface of the Fe3O4 magnetic sphere, a middle layer of macromolecules with controllable thickness and the PAMA polymer brush grafted with arginine are sequentially introduced, and the magnetic nano-sphere has a uniform particle size and a narrow particle size distribution, and can well respond to an externally applied magnetic field, so the magnetic nano-sphere grafted with the arginine polymer brush facilitates the enrichment and separation of phosphorylated polypeptides, and has a great significance to study the physiologicalbehavior protein phosphorylation process.

The invention provides hydrophilic polymer functional magnetic nanospheres as well as a preparation method and an application thereof to solve the problems that few magnetic hydrophilic matrix types exist, the preparation condition is harsh and operation is tedious. According to the technical scheme, the magnetic nanospheres take Fe3O4 as the core, after surface double bond functionalization, a hydrophilic polymer is formed on the surface of Fe3O4 through a precipitation polymerization reaction. The preparation method of the magnetic nanospheres comprises steps as follows: (1) preparation of Fe3O4 magnetic nanospheres with a solvothermal method; (2) surface double bond modification of the magnetic nanospheres; (3) preparation of hydrophilic functional magnetic nanospheres: the magnetic nanospheres are used as an adsorbent used for separation and enrichment of glycopeptide or glycoprotein. The magnetic nanospheres are prepared from simple components, are easy to produce, adopt mild reaction conditions, can be used for efficient and high-selectivity separation and enrichment, can be applied to enrichment of glycopeptide or glycoprotein in complex biological samples and have remarkable economic and social benefit.

The invention belongs to the fields of high molecular material and medical engineering, and in particular relates to a magnetic resonance imaging contrast agent for diagnosis and treatment and a preparation method thereof. Nanospheres encapsulating drugs are prepared by self assembly of a group-specific amphipathic block copolymer with drugs, metal ions are absorbed on the surfaces or membranous layers of the nanospheres through ionic bond or complexing action, and then magnetic particles are generated in situ by a chemical method to obtain the nanospheres useful for magnetic resonance imaging. The magnetic nanospheres composed of a polymer and inorganic particles have a stable structure, excellent water solubility and dispersibility, and good biocompatibility and biodegradability, can encapsulate abundant drugs for controllable release, and also can be used as a supersensitive magnetic resonance imaging contrast agent to be widely applied to diagnosis and treatment of disease.

The invention discloses magnetic nano-silicon-based aerobic granular sludge and a culture method and application thereof. The magnetic nano-silicon-based aerobic granular sludge contains magnetic nanospheres Fe3O4/SiO2. The culture method of the magnetic nano-silicon-based aerobic granular sludge includes the following step that the magnetic nanospheres Fe3O4/SiO2 are put into an active sludge system to form the magnetic nano-silicon-based aerobic granular sludge, and the magnetic nano-silicon-based aerobic granular sludge is used for wastewater treatment. The magnetic nano-silicon-based aerobic granular sludge has the advantages that formation is quick, the wastewater denitrification and dephosphorization capacity is high, magnetic nanometer materials in excess sludge are recycled conveniently, and long-time stable operation of the aerobic granular sludge is improved.

The invention discloses dopamine and dopamine metabolite based molecularly imprinted magnetic nanospheres as well as a preparation method and an application thereof. The method comprises the followingsteps: synthesizing carboxyl-functionalized magnetic nanospheres with a hydrothermal method; adding the carboxyl-functionalized magnetic nanospheres and two epinephrine metabolites to a phosphate buffer solution in a certain ratio to obtain a dual-template-carrier complex; stirring a separation product, functional monomer hyperbranched polyethyleneimine and crosslinking agent glutaraldehyde in the phosphate buffer solution for polymerization to obtain a polymer; separating a solid polymer out by externally applying a magnetic field; finally, performing reflux elution on the solid polymer, andperforming vacuum drying to obtain the dopamine and dopamine metabolite based molecularly imprinted magnetic nanospheres. The preparation method is simple, conditions are mild, and the prepared molecularly imprinted magnetic nanospheres are uniform in particle size, high in stability and good in water solubility, and can realize selective enrichment and detection of dopamine and 3,4-dihydroxyphenylacetic acid in complicated matrix of human fluid simultaneously and rapidly.

The invention relates to a preparation method of hollow superparamagnetic nanospheres. The preparation method comprises the following steps: (1) pretreating cellulose raw materials through alkali liquor, soaking in oxidizing salt solution, and then hydrolyzing to obtain carboxyl cellulose nanospheres; (2) dispersing the cellulose nanospheres into water, adding little organic solvent and uniformly stirring, then sequentially adding ferric salt and ferrous salt, dropping alkali to react, and collecting the product by a magnet, so as to obtain the superparamagnetic nanospheres; and (3) roasting the superparamagnetic nanospheres at high temperature under inert atmosphere, so as to obtain the hollow magnetic nanospheres. The preparation method is simple, convenient and green in preparation technology, free from expensive equipment, wide in source of raw materials for production, low in cost and easy for mass production; and the hollow magnetic nanospheres prepared by the preparation method are higher in superparamagnetic response, controllable in dimension and uniform in chemical components, and can be applied to the fields such as the sewage treatment filed, the catalysis field, the magnetofluid field, the microwave absorption filed, the drug carrier filed, the biological enzyme fixation field and the biosensor field.