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Preparation method of temperature sensitive magnetic western-blotting nanosphere

A technology of western blotting and magnetic nanospheres is applied in the fields of peptide preparation, nanotechnology, chemical instruments and methods, etc., to achieve the effects of good biocompatibility, uniform particle size and simple synthesis process.

Inactive Publication Date: 2012-07-04
NANKAI UNIV
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[0007] So far, the preparation method of temperature-sensitive magnetic western imprinted nanospheres has been rarely reported at home and abroad, and it is an innovative research in theory and method.

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  • Preparation method of temperature sensitive magnetic western-blotting nanosphere
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Embodiment Construction

[0029] A method for preparing temperature-sensitive magnetic Western blot nanospheres, comprising the steps of:

[0030] 1) Synthesize magnetic nanospheres with a particle size of 200nm by a hydrothermal method. The method is to place 1.35g of ferric chloride, 3.6g of sodium acetate and 35mL of ethylene glycol in a reaction kettle, react at 200°C for 6h, and use a magnet for solid-liquid After separation, the liquid was poured out, and the solid product was washed with ultrapure water to neutrality, dried in vacuum, figure 1 It is a transmission electron microscope image of magnetic nanospheres with a particle size of 200nm synthesized by the hydrothermal method;

[0031] 2) Coating a silicon layer with a thickness of 20nm on the surface of the above-mentioned magnetic nanospheres by gel-sol method, the method is to mix 200mg magnetic nanospheres, 80mL ethanol, 30mL ultrapure water, 3mL ammonia water and 2mL tetraethoxysilane Place in a three-necked flask, react at room tempe...

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Abstract

A preparation method of temperature sensitive magnetic western-blotting nanospheres comprises the following steps: synthesizing magnetic nanospheres with a particle size of 200 nm by a hydrothermal method; coating a silicon layer with a thickness of 20 nm on the surfaces of the magnetic nanospheres by a gel-sol method; adding the silicon-coated magnetic nanospheres, functional monomers, template molecules, and a cross-linking agent into a Tris-HCl buffer to obtain a reaction solution; performing polymerization of the reaction solution at a certain temperature to obtain polymers; eluting the polymers by an eluate to obtain the temperature sensitive magnetic western-blotting nanospheres. The advantages of the invention are that: the temperature sensitive magnetic western-blotting nanospheres combine the specific recognition of western blotting, the rapid separation performance of magnetic nanospheres in an external magnetic field, and the temperature responsibility of temperature sensitive materials; the preparation method is simple; the conditions are mild; the price is low; and a new feasible method is provided for the selective removal and enrichment of high-abundance protein components in a complex biological system.

Description

technical field [0001] The invention relates to the preparation technology of protein components, in particular to a preparation method of temperature-sensitive magnetic western imprinted nanospheres. Background technique [0002] At present, the research of proteomics has received extensive attention. Proteomics takes the existence and activity mode of all proteins in the cell as the research object, and understands the laws of life activities from a deeper level. The study of the relationship between protein structure and function and the difference of protein expression under different physiological and pathological conditions is called functional proteomics (mctional proteomics) research. The study of functional proteomics can help us understand the function of genes, discover proteins related to specific physiological and pathological conditions, and construct protein functional networks. The research scope of early proteomics mainly refers to the expression profile o...

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Application Information

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IPC IPC(8): C08F220/54C08F220/56C08F220/60C08F222/38C08F2/44C08K9/10C08K9/06C08K3/22C08J9/26C01G49/06B82Y40/00C07K1/14
Inventor 陈朗星高瑞霞何锡文张玉奎
Owner NANKAI UNIV
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