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Hydrophilic polymer functional magnetic nanospheres as well as preparation method and application thereof

A magnetic nano-functionalized technology, applied in the field of biomedicine, can solve the problems of few types of magnetic hydrophilic substrates, cumbersome operations, harsh preparation conditions, etc., and achieve high-efficiency and high-selectivity separation and enrichment, simple components, and reaction conditions. mild effect

Inactive Publication Date: 2017-02-22
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the above situation, in order to overcome the deficiencies of the prior art, the present invention provides a hydrophilic polymer functionalized magnetic nanosphere and its preparation method and application to solve the problem of few types of magnetic hydrophilic substrates, harsh preparation conditions, difficult operation cumbersome questions

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  • Hydrophilic polymer functional magnetic nanospheres as well as preparation method and application thereof

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Embodiment 1

[0019] Embodiment 1: The magnetic nano-microsphere is based on ferric oxide as the core, and after the double bond on its surface is functionalized, a hydrophilic polymer is formed on the surface of ferric oxide by precipitation polymerization.

[0020] The preparation method of the magnetic nano microspheres comprises the following steps:

[0021] (1) Synthesis of ferroferric oxide magnetic nano-microspheres by solvothermal method:

[0022] Dissolve 2.70g of ferric chloride hexahydrate in 140ml of ethylene glycol, and ultrasonicate for 5min to obtain a yellow transparent liquid, then add 7.70g of ammonium acetate and 0.80g of trisodium citrate, stir mechanically at room temperature for 1h, and place the obtained homogeneous solution in Stir in an oil bath at 170°C for 1 hour, heat to 200°C, react for 16 hours, cool to room temperature to obtain particles, wash the particles with absolute ethanol and water for 3 times, and dry at 60°C for 12 hours to obtain magnetic nanosphere...

Embodiment 2

[0027] Embodiment 2: The magnetic nano-microsphere is based on ferric iron tetroxide as the core, and after the double bond on its surface is functionalized, a hydrophilic polymer is formed on the surface of ferric ferric oxide by precipitation polymerization.

[0028] The preparation method of the magnetic nano microspheres comprises the following steps:

[0029] (1) Synthesis of ferroferric oxide magnetic nano-microspheres by solvothermal method:

[0030] Dissolve 2.0g of ferric chloride hexahydrate in 70ml of ethylene glycol, and ultrasonicate for 5min to obtain a yellow transparent liquid, then add 7.0g of ammonium acetate and 0.6g of trisodium citrate, stir mechanically at room temperature for 1h, and place the obtained homogeneous solution in Stir in an oil bath at 150°C for 1 hour, heat to 160°C, react for 12 hours, cool to room temperature to obtain particles, wash the particles with absolute ethanol and water for 3 times, and dry at 40°C for 12 hours to obtain magneti...

Embodiment 3

[0035] Embodiment 3: The magnetic nano-microsphere is based on ferric oxide as the core, and after the double bond on its surface is functionalized, a hydrophilic polymer is formed on the surface of ferric oxide by precipitation polymerization.

[0036] The preparation method of the magnetic nano microspheres comprises the following steps:

[0037] (1) Synthesis of ferroferric oxide magnetic nano-microspheres by solvothermal method:

[0038] Dissolve 3.0g of ferric chloride hexahydrate in 140ml of ethylene glycol, and ultrasonicate for 5min to obtain a yellow transparent liquid, then add 8.0g of ammonium acetate and 1.0g of trisodium citrate, stir mechanically at room temperature for 1h, and place the obtained homogeneous solution in Stir in an oil bath at 190°C for 2 hours, heat to 200°C, react for 24 hours, cool to room temperature to obtain particles, wash the particles with absolute ethanol and water for 3 times, and dry at 60°C for 12 hours to obtain magnetic nanospheres;...

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Abstract

The invention provides hydrophilic polymer functional magnetic nanospheres as well as a preparation method and an application thereof to solve the problems that few magnetic hydrophilic matrix types exist, the preparation condition is harsh and operation is tedious. According to the technical scheme, the magnetic nanospheres take Fe3O4 as the core, after surface double bond functionalization, a hydrophilic polymer is formed on the surface of Fe3O4 through a precipitation polymerization reaction. The preparation method of the magnetic nanospheres comprises steps as follows: (1) preparation of Fe3O4 magnetic nanospheres with a solvothermal method; (2) surface double bond modification of the magnetic nanospheres; (3) preparation of hydrophilic functional magnetic nanospheres: the magnetic nanospheres are used as an adsorbent used for separation and enrichment of glycopeptide or glycoprotein. The magnetic nanospheres are prepared from simple components, are easy to produce, adopt mild reaction conditions, can be used for efficient and high-selectivity separation and enrichment, can be applied to enrichment of glycopeptide or glycoprotein in complex biological samples and have remarkable economic and social benefit.

Description

technical field [0001] The invention relates to biomedicine, in particular to a hydrophilic polymer functionalized magnetic nano-microsphere and its preparation method and application. Background technique [0002] Glycoproteins play an important role in the physiological process of cells. Glycoproteins are involved in cell adhesion, operation metabolism, signal transduction, endocytosis, receptor activation and other processes, so the study of glycoproteomics is helpful to explore the mechanism of cancer , which is of great significance to the discovery and prevention of cancer. Biomass spectrometry based on soft ionization has become an important tool for proteomics research. At present, the shotgun strategy is the most widely used proteomics analysis strategy, mainly including: obtaining biological samples, extracting, labeling, enzymatically hydrolyzing, Separation and enrichment, etc., identify the peptides by mass spectrometry, and obtain mass spectrometry data for an...

Claims

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Application Information

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IPC IPC(8): C08F292/00C08F220/38C08F222/38B01J13/02B01J20/281B01J20/28B01J20/26
CPCB01J13/02B01J20/264B01J20/28009B01J20/28021B01J20/281C08F292/00C08F220/387
Inventor 纪永升吕瑞红钟铮丹阳王一硕胡媛媛范涛
Owner HENAN UNIV OF CHINESE MEDICINE
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