The present invention is a preparation method of the trans-isoamyl rubber complex, the product and the application. The trans-isoamyl rubber complex is made by doing the binary, ternary, quaternary or multi-blend and total vulcanization with the synthetic reversing formula 1, 4-polyisoprene and the general rubber such as the natural rubber, the styrene-butadiene rubber, the polybutadiene rubber, the cis-polyisoprene rubber, the ethylene propylene rubber, the nitrile rubber, the chloroprene rubber and so on, or with the special rubber. The TPI is used to load the titanium catalyst to catalyze the synthesis of the polyisoprene body through the methods of deposition and polymerization. The structural content of the formula-1, 4 is equal to or more than 90 percent. The present invention makes a rubber complex containing the TPI to overcome the insufficiency of the existing rubber complex in the dynamic performance and to reach the balance between the rolling resistance, the traction performance and the wear performance; the trans-isoamyl rubber complex of the present invention is suitable for the rubber products used in the tire surface and side, the shock absorber product for trains and vehicles, the V-belt, the conveyor belt and in other dynamic uses.