Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing superparamagnetic microspheres with surfactant-based nuclear shell

A superparamagnetic and microsphere technology, which is applied in the preparation of surface carboxyl core-shell superparamagnetic polymer microspheres, can solve the problems of less surface functional groups, low magnetic content, leaching of magnetic particles, etc., and achieve monodispersity Good, controllable magnetic content

Active Publication Date: 2018-08-31
SUZHOU WEIDU BIOTECH CO LTD
View PDF4 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the magnetic polymer microspheres prepared by this method often have the disadvantages of poor monodispersity, low magnetic content, and few surface functional groups, which limits the application of this method in the preparation of commercial magnetic polymer microspheres.
[0004] As early as 1987, a foreign company developed a method of in-situ deposition of magnetic particles on the surface of porous microspheres to obtain monodisperse superparamagnetic microspheres, but this method has inherent defects. Magnetic particles will leach over time, which is not conducive to subsequent applications

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing superparamagnetic microspheres with surfactant-based nuclear shell
  • Method for preparing superparamagnetic microspheres with surfactant-based nuclear shell
  • Method for preparing superparamagnetic microspheres with surfactant-based nuclear shell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Weigh 900 g of absolute ethanol, 100 g of pure water, and 10 g of polyvinylpyrrolidone into a 2L four-necked flask equipped with mechanical stirring, and dissolve them by mechanical stirring. 100 g of glycidyl methacrylate monomer was added thereto. The temperature of the water bath was set at 70° C., and when the temperature was raised to 70° C., 100 g of ethanol solution in which 1.2 g of azobisisobutyronitrile was dissolved was quickly added to the reaction system. Mechanical stirring at 200 rpm, water bath at 70°C, and polymerization reaction for 24 hours. The polyglycidyl methacrylate seed microspheres were obtained by centrifugal washing with pure water.

[0027] Weigh 20 g of the seed microspheres prepared above, and disperse them in 1500 g of 0.5% polyvinyl alcohol solution by mass fraction to form dispersion A, which is added to a 2L four-necked flask equipped with mechanical stirring, in a water bath of 35°C, and mechanical stirring at 120 rpm. Weigh 112g of...

Embodiment 2

[0033]Weigh 900 g of absolute ethanol and 10 g of polyvinylpyrrolidone into a 2L four-necked flask equipped with mechanical stirring, and dissolve them by mechanical stirring. 60 g of glycidyl methacrylate monomer was added thereto. The temperature of the water bath was set at 70° C., and when the temperature was raised to 70° C., 100 g of ethanol solution in which 1.2 g of azobisisobutyronitrile was dissolved was quickly added to the reaction system. Mechanical stirring at 200 rpm, water bath at 70°C, and polymerization reaction for 24 hours. The polyglycidyl methacrylate seed microspheres were obtained by ethanol centrifugal washing.

[0034] Weigh 20 g of the seed microspheres prepared above, and disperse them in 1500 g of polyvinyl alcohol solution with a mass fraction of 0.5% to form dispersion A, and place it in a 2L four-necked flask equipped with mechanical stirring, in a water bath of 35°C, and mechanical stirring at 120rpm . Weigh 84g of glycidyl methacrylate, 20g...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Sizeaaaaaaaaaa
Average sizeaaaaaaaaaa
Saturation magnetizationaaaaaaaaaa
Login to View More

Abstract

The invention relates to a method for preparing superparamagnetic microspheres with a surfactant-based nuclear shell, which comprises the following steps of: preparing monodispersed polyglycidyl methacrylate microspheres by a free radical polymerization of monomeric polyglycidyl methacrylate in the presence of an initiator and an emulsifying agent or a dispersing agent; preparing monodispersed porous polyglycidyl methacrylate microspheres with larger size by a one-step seed swelling polymerization; adding divalent and trivalent iron to stir, raising the temperature of the system, adding excessive concentrated ammonia water, and preparing monodispersed superparamagnetic microspheres in situ by an iron salt deposition and an alkaline coprecipitation method; the magnetic microspheres are subjected to surface carboxylic acid modification by distillation precipitation polymerization. The superparamagnetic microspheres with the surfactant-based nuclear shell have the advantages of good mono-dispersity, controllable magnetic content and the like, the microspheres have abundant carboxylic acid groups on the surface, and after a subsequent binding to biological ligands, and can be widely applied to the fields of biomedical analysis and detection, such as immobilized enzyme and cell separation, protein purification, immunoassay, and the like.

Description

technical field [0001] The invention relates to a preparation method of magnetic polymer microspheres, in particular, it shows a preparation method of surface carboxyl core-shell superparamagnetic polymer microspheres. Background technique [0002] In recent years, with its unique advantages, micro-nano technology has gradually penetrated into various fields and developed rapidly. As the basis of micro-nano technology, research on micro-nano materials has attracted much attention, especially composite micro-nano materials. Magnetic polymer microspheres are an example. They have both the magnetism of inorganic magnetic materials and the modifiability of organic polymers. They have a wide range of applications in the fields of immobilized enzymes, immunoassays, cell separation, protein purification, and magnetically targeted drug carriers. prospect. [0003] At present, the preparation of magnetic polymer microspheres mainly adopts the polymer polymerization method in the pr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08F265/04C08F220/32C08F222/14C08F8/32C08K3/22C08J9/00
CPCC08F8/32C08F220/32C08F220/325C08F265/04C08J9/00C08J9/0066C08J2333/14C08K3/22C08K2003/2265C08F222/102
Inventor 宋孟杰杜德状
Owner SUZHOU WEIDU BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com