Method for preparing superparamagnetic microspheres with surfactant-based nuclear shell

A superparamagnetic and microsphere technology, which is applied in the preparation of surface carboxyl core-shell superparamagnetic polymer microspheres, can solve the problems of less surface functional groups, low magnetic content, leaching of magnetic particles, etc., and achieve monodispersity Good, controllable magnetic content

A superparamagnetic and microsphere technology, which is applied in the preparation of surface carboxyl core-shell superparamagnetic polymer microspheres, can solve the problems of less surface functional groups, low magnetic content, leaching of magnetic particles, etc., and achieve monodispersity Good, controllable magnetic content

CN108467461AActive Publication Date: 2018-08-31SUZHOU WEIDU BIOTECH CO LTD
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  • Method for preparing superparamagnetic microspheres with surfactant-based nuclear shell
  • Method for preparing superparamagnetic microspheres with surfactant-based nuclear shell
  • Method for preparing superparamagnetic microspheres with surfactant-based nuclear shell

Examples

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Embodiment 1

[0026] Weigh 900 g of absolute ethanol, 100 g of pure water, and 10 g of polyvinylpyrrolidone into a 2L four-necked flask equipped with mechanical stirring, and dissolve them by mechanical stirring. 100 g of glycidyl methacrylate monomer was added thereto. The temperature of the water bath was set at 70° C., and when the temperature was raised to 70° C., 100 g of ethanol solution in which 1.2 g of azobisisobutyronitrile was dissolved was quickly added to the reaction system. Mechanical stirring at 200 rpm, water bath at 70°C, and polymerization reaction for 24 hours. The polyglycidyl methacrylate seed microspheres were obtained by centrifugal washing with pure water.

[0027] Weigh 20 g of the seed microspheres prepared above, and disperse them in 1500 g of 0.5% polyvinyl alcohol solution by mass fraction to form dispersion A, which is added to a 2L four-necked flask equipped with mechanical stirring, in a water bath of 35°C, and mechanical stirring at 120 rpm. Weigh 112g of...

Embodiment 2

[0033]Weigh 900 g of absolute ethanol and 10 g of polyvinylpyrrolidone into a 2L four-necked flask equipped with mechanical stirring, and dissolve them by mechanical stirring. 60 g of glycidyl methacrylate monomer was added thereto. The temperature of the water bath was set at 70° C., and when the temperature was raised to 70° C., 100 g of ethanol solution in which 1.2 g of azobisisobutyronitrile was dissolved was quickly added to the reaction system. Mechanical stirring at 200 rpm, water bath at 70°C, and polymerization reaction for 24 hours. The polyglycidyl methacrylate seed microspheres were obtained by ethanol centrifugal washing.

[0034] Weigh 20 g of the seed microspheres prepared above, and disperse them in 1500 g of polyvinyl alcohol solution with a mass fraction of 0.5% to form dispersion A, and place it in a 2L four-necked flask equipped with mechanical stirring, in a water bath of 35°C, and mechanical stirring at 120rpm . Weigh 84g of glycidyl methacrylate, 20g...

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Abstract

The invention relates to a method for preparing superparamagnetic microspheres with a surfactant-based nuclear shell, which comprises the following steps of: preparing monodispersed polyglycidyl methacrylate microspheres by a free radical polymerization of monomeric polyglycidyl methacrylate in the presence of an initiator and an emulsifying agent or a dispersing agent; preparing monodispersed porous polyglycidyl methacrylate microspheres with larger size by a one-step seed swelling polymerization; adding divalent and trivalent iron to stir, raising the temperature of the system, adding excessive concentrated ammonia water, and preparing monodispersed superparamagnetic microspheres in situ by an iron salt deposition and an alkaline coprecipitation method; the magnetic microspheres are subjected to surface carboxylic acid modification by distillation precipitation polymerization. The superparamagnetic microspheres with the surfactant-based nuclear shell have the advantages of good mono-dispersity, controllable magnetic content and the like, the microspheres have abundant carboxylic acid groups on the surface, and after a subsequent binding to biological ligands, and can be widely applied to the fields of biomedical analysis and detection, such as immobilized enzyme and cell separation, protein purification, immunoassay, and the like.

Description

technical field [0001] The invention relates to a preparation method of magnetic polymer microspheres, in particular, it shows a preparation method of surface carboxyl core-shell superparamagnetic polymer microspheres. Background technique [0002] In recent years, with its unique advantages, micro-nano technology has gradually penetrated into various fields and developed rapidly. As the basis of micro-nano technology, research on micro-nano materials has attracted much attention, especially composite micro-nano materials. Magnetic polymer microspheres are an example. They have both the magnetism of inorganic magnetic materials and the modifiability of organic polymers. They have a wide range of applications in the fields of immobilized enzymes, immunoassays, cell separation, protein purification, and magnetically targeted drug carriers. prospect. [0003] At present, the preparation of magnetic polymer microspheres mainly adopts the polymer polymerization method in the pr...

Claims

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Application Information

Patent Timeline
31 Aug 2018
Publication
CN108467461A
IPC
C08F265/04; C08F220/32; C08F222/14; C08F8/32; C08K3/22; C08J9/00
CPC
C08F8/32; C08F220/32; C08F220/325; C08F265/04; C08J9/00; C08J9/0066; C08J2333/14; C08K3/22
Inventors
宋孟杰; 杜德状