The invention discloses a preparation method of molecularly imprinted
polymeric microspheres for 2,4,6-trinitrophenol detection. The preparation method is characterized in that polynitrophenol derivatives-imprinted molecules generated through reaction between 2,4,6-trinitrophenol and N,N'-diisopropyl
carbodiimide have luminous yellow
fluorescent light, the
fluorescent light of the imprinted molecules has open and close characteristics under an acid-base condition, the imprinted molecules located in an imprinting layer of the molecularly imprinted
polymeric microspheres can be eluted, a hole structure which is complementary with the structure, the size and the functional groups of the imprinted molecules is formed in the imprinting layer,
polymeric microspheres of which the imprinted molecules are eluted have specific recognition sites on target
analyte molecules, target molecules enter the specific recognition sites, selective recognition on the target
analyte molecules is realized, sensitivity detection of the target
analyte molecules can be realized through opening and closing of the
fluorescent light of the target molecules, the specific surface area of the molecularly imprintedmicrospheres is large, and space in which the recognition sites are formed is stable in structure, has more effective sites, and is capable of carrying out high-selective recognition and high-sensitive detection on the target molecules.