Carrier for immobilizing enzyme, use thereof, and carrier immobilized with enzyme

A technology for immobilizing enzymes and carriers, which is applied in the fields of enzyme-immobilized carriers, enzyme-immobilized carriers, and polymer microspheres, and can solve the problems of poor mass transfer performance of synthetic polymer materials.

Active Publication Date: 2011-11-30
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the poor mass transfer properties of synthetic polymers is a disadvantage of their use as carriers for immobilized enzymes

Method used

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  • Carrier for immobilizing enzyme, use thereof, and carrier immobilized with enzyme
  • Carrier for immobilizing enzyme, use thereof, and carrier immobilized with enzyme
  • Carrier for immobilizing enzyme, use thereof, and carrier immobilized with enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 [the most probable pore diameter is the ultra-large pore PST microsphere of 313nm]

[0046] Super macroporous polystyrene (PST) microspheres ( figure 2 ), the pores of the carrier less than 90nm account for 8% of the total porosity of the microspheres, the pores of more than 90nm and less than 100nm account for 12% of the total porosity of the microspheres; the pores of more than 100nm and less than 300nm account for 39% of the total porosity of the microspheres, The pores above 300nm and less than 500nm accounted for 35% of the total porosity of the microspheres, the pores above 500nm and less than 800nm ​​accounted for 5% of the total porosity of the microspheres, and the most probable pore diameter was 313nm. The average particle size is 100 microns, the porosity is 60%, and the specific surface area is 34m 2 / g, the degree of crosslinking is 12%.

Embodiment 2

[0047] Embodiment 2 [the most probable aperture is 104nm PST microsphere)

[0048] Super-macroporous polystyrene microspheres, the pores of the carrier less than 90nm account for 10% of the total pores of the microspheres, the pores of more than 90nm and less than 100nm account for 30% of the total pores, the pores of more than 100nm and less than 300nm account for 40% of the total pores of the microspheres, 300nm The pores above and less than 500nm account for 10% of the total porosity of the microspheres, and the most probable pore diameter is 104nm. The average particle size is 40 microns, the porosity is 60%, and the specific surface area is 86m 2 / g, the degree of crosslinking is 25%.

Embodiment 3

[0049] Embodiment 3 [most probable aperture is 400nm PGMA microsphere]

[0050] Super-macroporous polyglycidyl methacrylate (PGMA) microspheres ( image 3 ), the pores of the carrier less than 90nm account for 4% of the total porosity of the microspheres, the pores of more than 90nm and less than 100nm account for 8% of the total porosity of the microspheres; the pores of more than 100nm and less than 300nm account for 30% of the total porosity of the microspheres, The pores above 300nm and less than 500nm account for 45% of the total porosity of the microspheres, the pores above 500nm and less than 800nm ​​account for 10% of the total porosity of the microspheres, and the pores of 800-1000nm account for 3% of the total porosity of the microspheres. The available aperture is 400nm. The average particle size is 30 microns, the porosity is 70%, and the specific surface area is 29m 2 / g, the degree of crosslinking is 25%.

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Abstract

The invention relates to a novel carrier with good permeability for immobilizing enzymes. The carrier is a polymer microsphere not only with small and medium pores smaller than 90nm, but also with large pores of hundreds of nanometers. The carrier can be used for both single-enzyme immobilization and multiple-enzyme immobilization. The utilization rate of the microsphere surface can reach 70%-100%. The present invention also relates to the use of the carrier for immobilizing enzymes in immobilizing enzymes and the carrier immobilized with enzymes. Because the enzyme can be immobilized inside the carrier and can be well protected by the carrier, it has good stability, activity and reusability, and is especially suitable for column packing for continuous reactions.

Description

technical field [0001] The invention belongs to the field of biochemical industry and relates to a carrier for immobilizing enzymes, in particular to a polymer microsphere with super large pores as a carrier for immobilizing enzymes. The present invention also relates to the use of the carrier for immobilizing enzymes in immobilizing enzymes and the carrier immobilized with enzymes. Background technique [0002] As a natural polymer catalyst, enzyme has a very broad application prospect in food processing, medicine and other industries due to its high selectivity, catalytic reaction, mild conditions, and no pollution. However, the disadvantages of free enzymes such as instability and easy deformation make it difficult to be more widely used in industry. In addition, the separation and purification of enzymes in the reaction system also increases the production cost. Therefore, the research on immobilized enzymes has attracted more and more attention. After decades of devel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/08C12N11/04
Inventor 马光辉苏志国李燕周炜清
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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