Preparation method of molecularly imprinted polymeric microspheres for 2,4,6-trinitrophenol detection
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A technology of molecularly imprinted microspheres and trinitrophenol, applied in the field of detection 2
Active Publication Date: 2018-07-06
霍尔姆斯(北京)生物科技有限公司
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[0010] Although the above methods have their own characteristics, there is no report in the literature that the target molecule itself has fluorescent properties, and the target molecule with yellow fluorescence enters the recognition site through its own optical signal sensitive output.
At the same time, there is no report in the literature that the polynitrophenol derivatives with bright yellow fluorescence produced by the reaction of 2,4,6-trinitrophenol and N,N'-diisopropylcarbodiimide are imprinted into polymer microparticles. Spheres, imprinted derivatives that enter the recognition site, enable reporting of recognition and detection by switching on and off of autofluorescence
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[0052] A preparation method of molecularly imprinted polymer microspheres for detection of 2,4,6-trinitrophenol, characterized in that: 2,4,6-trinitrophenol and N,N'-diisopropyl The polynitrophenol derivatives generated by the carbodiimide reaction have bright yellow fluorescence, and their fluorescence has open-close characteristics under acid-base conditions. The molecularly imprinted polymer microspheres are eluted in the imprinted layer. Molecules, the inner formation of the imprinting layer has a cavity structure that is complementary to the structure, size and functional group of the imprinted molecule, and the polymer microspheres that elute the imprinted molecule have specific recognition sites for the target analyte molecule. Molecular selective identification and detection, the above-mentioned preparation process includes the following four steps:
[0053] The first step is the synthesis of 2,4,6-trinitrophenol derivatives: Weigh 0.4-0.6 g of the treated 2,4,6-trinit...
specific Embodiment
[0058] 2,4,6-Trinitrophenol and N,N'-diisopropylcarbodiimide are produced by nucleophilic addition reaction of polynitrophenol derivatives. After separation and purification, 2,4,6- Molecularly imprinted polymer microspheres were prepared by copolymerization of trinitrophenol derivative PAD1 with functional monomers, cross-linking agents and initiators. After eluting the template molecule PAD1, polymer microspheres with selective recognition and fluorescence signal output for PAD1 were obtained .
[0059] The first step is the synthesis of 2,4,6-trinitrophenol derivatives: Weigh 0.5 g of the treated 2,4,6-trinitrophenol into a 100 mL three-necked flask, and then add 7.5 mL of dichloromethane After ultrasonic dispersion, 0.3 g of N,N'-diisopropylcarbodiimide was added, and the solution immediately turned reddish-brown. It was stirred for 2 h under nitrogen atmosphere. Due to the volatilization of dichloromethane, the solution became viscous. dried in a vacuum drying oven at 40...
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Abstract
The invention discloses a preparation method of molecularly imprinted polymeric microspheres for 2,4,6-trinitrophenol detection. The preparation method is characterized in that polynitrophenol derivatives-imprinted molecules generated through reaction between 2,4,6-trinitrophenol and N,N'-diisopropyl carbodiimide have luminous yellow fluorescent light, the fluorescent light of the imprinted molecules has open and close characteristics under an acid-base condition, the imprinted molecules located in an imprinting layer of the molecularly imprinted polymeric microspheres can be eluted, a hole structure which is complementary with the structure, the size and the functional groups of the imprinted molecules is formed in the imprinting layer, polymeric microspheres of which the imprinted molecules are eluted have specific recognition sites on target analyte molecules, target molecules enter the specific recognition sites, selective recognition on the target analyte molecules is realized, sensitivity detection of the target analyte molecules can be realized through opening and closing of the fluorescent light of the target molecules, the specific surface area of the molecularly imprintedmicrospheres is large, and space in which the recognition sites are formed is stable in structure, has more effective sites, and is capable of carrying out high-selective recognition and high-sensitive detection on the target molecules.
Description
technical field [0001] The invention relates to the field of material science, in particular to a preparation method for detecting 2,4,6-trinitrophenol molecular imprint. Background technique [0002] International relations in today's world are complex and changeable, and terrorist and violent incidents such as bombings occur frequently, which seriously endanger the lives and property safety of people all over the world and social stability, and pose a threat to the security and stability of countries and regions. Therefore, the detection and analysis of explosives has attracted great attention from research institutions in various countries, trying to predict the existence of explosives and reduce the harm of explosives to humans through analysis and detection technology. 2,4,6-trinitrophenol (2,4,6-trinitrophenol, TNP) belongs to phenolic substance, also known as picric acid (picric acid, PA). Its raw materials are easy to obtain, its preparation is simple, and its explo...
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