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Molecularly imprinted polymer sensing material suitable for biological samples and preparation method of sensing material

A molecularly imprinted, polymer technology, applied in material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of time-consuming and limit the practical application of molecularly imprinted polymers.

Active Publication Date: 2017-01-11
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the detection process of organic molecules requires not only high-speed centrifugation of the biological sample solution of the molecularly imprinted polymer, but also the precipitation of the protein in the centrifuged supernatant for quantitative analysis, so this process is very time-consuming, which greatly Practical applications of such molecularly imprinted polymers are limited

Method used

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  • Molecularly imprinted polymer sensing material suitable for biological samples and preparation method of sensing material
  • Molecularly imprinted polymer sensing material suitable for biological samples and preparation method of sensing material
  • Molecularly imprinted polymer sensing material suitable for biological samples and preparation method of sensing material

Examples

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Effect test

example 1

[0035] Add 0.834mmol of tetracycline Tc into a 100mL round-bottomed flask filled with 60mL of acetonitrile / dimethylformamide (4:1, volume / volume), stir it with a magnet to dissolve it completely, then add 0.834mmol of methacrylic acid, 2.501mmol Ethylene glycol dimethacrylate, 0.0834mmol polymerizable fluorescent monomer (2-hydroxyethyl methacrylate) 9-anthracene ester, 0.0552mmol small molecule chain transfer agent cumyl dithiobenzoate (CDB), 0.0342mmol poly-2-hydroxyethyl methacrylate macromolecular chain transfer agent (M n，NMR =5140) and 0.0566 mmol of azobisisobutyronitrile (AIBN). After deoxygenating with argon for 30 minutes, the reaction system was sealed, placed in a constant temperature oil bath at 60°C, reacted for 24 hours, and centrifuged to obtain the reaction product.

[0036] The reaction product was washed successively with methanol / acetic acid (9:1, volume / volume) and methanol until the template-free molecules were washed out. After drying, freeze-dry in va...

example 2

[0039] Add 0.834mmol of tetracycline Tc into a 100mL round-bottomed flask filled with 60mL of acetonitrile / dimethylformamide (4:1, volume / volume), stir it with a magnet to dissolve it completely, then add 0.834mmol of methacrylic acid, 2.501mmol Ethylene glycol dimethacrylate, 0.0834mmol polymerizable fluorescent monomer (2-hydroxyethyl methacrylate) 9-anthracene ester, 0.0552mmol small molecule chain transfer agent cumyl dithiobenzoate (CDB), 0.0342mmol poly-2-hydroxyethyl methacrylate macromolecular chain transfer agent (M n，NMR =3610) and 0.0566 mmol of azobisisobutyronitrile (AIBN). After deoxygenating with argon for 30 minutes, the reaction system was sealed, placed in a constant temperature oil bath at 60°C, reacted for 24 hours, and centrifuged to obtain the reaction product.

[0040] The reaction product was washed successively with methanol / acetic acid (9:1, volume / volume) and methanol until the template-free molecules were washed out. After drying, freeze-dry in va...

example 3

[0043] Add 0.834mmol of tetracycline Tc into a 100mL round-bottomed flask filled with 60mL of acetonitrile / dimethylformamide (4:1, volume / volume), stir it with a magnet to dissolve it completely, then add 0.834mmol of methacrylic acid, 2.501mmol Ethylene glycol dimethacrylate, 0.0834mmol polymerizable fluorescent monomer (2-hydroxyethyl methacrylate) 9-anthracene ester, 0.0552mmol small molecule chain transfer agent cumyl dithiobenzoate (CDB), 0.0342mmol poly-2-hydroxyethyl methacrylate macromolecular chain transfer agent (M n，NMR =2610) and 0.0566 mmol of azobisisobutyronitrile (AIBN). After deoxygenating with argon for 30 minutes, the reaction system was sealed, placed in a constant temperature oil bath at 60°C, reacted for 24 hours, and centrifuged to obtain the reaction product.

[0044] The reaction product was washed successively with methanol / acetic acid (9:1, volume / volume) and methanol until the template-free molecules were washed out. After drying, freeze-dry in va...

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Abstract

The invention relates to a molecularly imprinted polymer photochemical sensing micro / nanoparticle capable of rapidly and quantitatively detecting an organic small molecule in a biological sample and a preparation method of the micro / nanoparticle. The molecularly imprinted polymer micro / nanoparticle has a cross-linking degree of 50% or more and a particle size of 0.01-5 micrometers, and the molecularly imprinted polymer micro / nanoparticle is grafted with a hydrophilic polymer brush on the surface and has a fluorescent property. The molecularly imprinted polymer micro / nanoparticle is synthesized in one step by the use of a hydrophilic macromolecule chain transfer agent induced reversible addition-cleavage chain transfer (RAFT) precipitation polymerization technique in the presence of polymerizable fluorophores. The molecularly imprinted polymer photochemical sensing micro / nanoparticle has the advantages of simple synthesis method, wide application range, pure product and the like, and has broad application prospects in the fields of food safety, environmental monitoring, clinical diagnosis and the like.

Description

[0001] Technical field [0002] The invention relates to a molecularly imprinted polymer photochemical sensing material capable of rapidly and quantitatively detecting small organic molecules in biological samples and a preparation method thereof, in particular to a fluorescent molecularly imprinted surface with a hydrophilic polymer brush Polymer micro / nanoparticles and methods for their preparation. Background technique [0003] Molecular imprinting technology is a simple and efficient new method for preparing synthetic receptors with specific molecular recognition sites (H. Zhang, L. Ye, K. Mosbach, J. Mol. Recognit. 2006, 19, 248-259 ). Molecularly Imprinted Polymers (MIPs) obtained from it have the advantages of good specific molecular recognition performance, high heat resistance and chemical stability, simple preparation process, and low cost. Phase extraction, immunoassay, bionic sensors, artificial enzymes, organic synthesis and drug delivery have shown broad applic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F265/04C08F222/14C08F220/06C08F220/28C08F2/44C08J9/26G01N21/64
Inventor 张会旗牛慧杨雅琼
Owner NANKAI UNIV
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