1results about How to "Uniform polymerization" patented technology

The application discloses a plastic embedding agent Big Cut resin, which is composed of a crosslinking agent and a non-reactive liquid auxiliary agent in a mass ratio of 3:7 to 7:3. The application further discloses a preparation method of the plastic embedding agent, which comprises the steps of mixing the crosslinking agent and the non-reactive liquid auxiliary agent in the mass percentage to obtain a mixture, and adding an initiator into the mixture to obtain the Big Cut resin. The application further discloses a plastic embedding method suitable for thick cutting of a large-volume sample, which embeds a biological tissue sample by using the Big Cut resin. The embedding method can realize uniform and stable polymerization of the large-volume resin, improve the cutting performance of the sample after embedding, meet the thick cutting requirement of an fMOST system, has the advantages of transparent depth imaging and adjustable refractive index, and realizes high-throughput imaging.