Nano-hydroxyapatite/carboxymethyl chitosan/poly(lactic-co-glycolic acid) micro-nano hybrid drug-loaded scaffold and bionic preparation method thereof
A nano-hydroxyapatite, carboxymethyl chitosan technology, applied in medical science, tissue regeneration, prosthesis, etc., can solve the problem of poor hydrophilicity and biocompatibility, lack of cell recognizable sites, lack of Biological activity and other issues, to achieve the effect of improving biological activity, good biocompatibility, and achieving complementary functional advantages
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[0039] Example 1
[0040] 1) Disperse 0.075 g of nano-hydroxyapatite in 20 mL of deionized water, and sonicate for 10 minutes to make the dispersion uniform;
[0041] 2) Add 0.2g of carboxymethyl chitosan to it and stir for 1 hour with a magnetic stirrer to form a uniform water phase (continuous phase);
[0042] 3) Take 136 µg of icariin and add it to the above water phase to prepare a molar concentration of 10 -5 Icariin solution of M;
[0043] 4) Dissolve 0.32g of polylactic acid glycolic acid in 8mL of dichloromethane to prepare the oil phase (dispersed phase) of the emulsion;
[0044] 5) Take 5mL of the emulsion water phase and 2mL of the oil phase, add them to the mold, add 14.5 µL of glutaraldehyde to the mold with the molar ratio of amino group to aldehyde group at 1:1, and use Ultra Turrax T25 high-speed emulsifier 3 Emulsify at rpm / min, the emulsification time is 1min;
[0045] 6) Put the emulsion at room temperature for 12h to volatilize the solvent, then transfer it to -10~-8...
Example Embodiment
[0046] Example 2
[0047] 1) Disperse 0.15g of nano-hydroxyapatite in 20mL of deionized water, and ultrasound for 10min to make the dispersion uniform;
[0048] 2) Add 0.2g of carboxymethyl chitosan to it and stir for 1 hour with a magnetic stirrer to form a uniform water phase (continuous phase);
[0049] 3) Take 136 µg of icariin and add it to the above water phase to prepare a molar concentration of 10 -5 Icariin solution of M;
[0050] 4) Dissolve 0.32g polylactic acid glycolic acid in 8mL dichloromethane to prepare the oil phase (dispersed phase) of the emulsion;
[0051] 5) Take 5mL of the emulsion water phase and 2mL of the oil phase, add them to the mold, add 14.5 µL of glutaraldehyde to the mold with the molar ratio of amino group to aldehyde group at 1:1, and use Ultra Turrax T25 high-speed emulsifier 3 Emulsify at rpm / min, the emulsification time is 1min;
[0052] 6) Place the emulsion at room temperature for 12h to volatilize the solvent, then transfer it to -10~-80℃ for fre...
Example Embodiment
[0053] Example 3
[0054] 1) Disperse 0.3 g of nano-hydroxyapatite in 20 mL of deionized water, and sonicate for 10 minutes to make the dispersion uniform;
[0055] 2) Add 0.2g of carboxymethyl chitosan to it and stir for 1 hour with a magnetic stirrer to form a uniform water phase (continuous phase);
[0056] 3) Take 136 µg of icariin and add it to the above water phase to prepare a molar concentration of 10 -5 Icariin solution of M;
[0057] 4) Dissolve 0.32g polylactic acid glycolic acid in 8mL dichloromethane to prepare the oil phase (dispersed phase) of the emulsion;
[0058] 5) Take 5mL of the emulsion water phase and 2mL of the oil phase, add them to the mold, add 14.5 µL of glutaraldehyde to the mold with the molar ratio of amino group to aldehyde group at 1:1, and use Ultra Turrax T25 high-speed emulsifier 3 Emulsify at rpm / min, the emulsification time is 1min;
[0059] 6) Place the emulsion at room temperature for 12h to volatilize the solvent, then transfer it to -10~-80℃ for...
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