23results about How to "High drug loading rate" patented technology

The invention provides a preparation method of a water and fertilizer retention type soil amendment, and belongs to the technical field of soil amendments. Biomass polysaccharide with abundant carboxyl functional groups is combined with metal ions to form a three-dimensional network structure capable of effectively coating soil nutrients; meanwhile, the soil conditioner is endowed with obvious pH responsiveness, and targeted remediation can be performed according to different soil conditions; the biomass-based soil conditioner provided by the invention has excellent water-retaining property and good slow-release performance, can improve the soil moisture content, prolong the retention time of soil moisture, and slowly release inclusions while releasing the system moisture, thereby achieving the effect of improving the soil fertility and realizing the improvement of the water-retaining and fertilizer-retaining capabilities of the soil. Meanwhile, the soil conditioner prepared by the invention can also improve the drought stress resistance of crops.

The invention discloses a GSH response type cell membrane bionic nano-drug, a preparation method and application, the cell membrane bionic nano-drug comprises cell membrane vesicles serving as a carrier, the cell membrane vesicles are prepared by performing cell membrane extraction and membrane extrusion on immune cells of which the membrane surfaces overexpress azide, and the cell membrane vesicles serve as a carrier. The azide on the cell membrane vesicles and the bio-coupling compound are bonded through a click chemical reaction to form-SH groups, and the-SH groups and the small-molecule inhibitor are combined together in a disulfide bond mode through the cross-linking agent. The drug is connected with a small-molecule inhibitor through a disulfide bond, so that the drug loading rate is relatively higher, the stability is better, and drug leakage in the blood circulation process is relatively less; the small-molecule inhibitor can be completely released only in a tumor site microenvironment, so that the tumor m6A methylation level is reduced, the EMT state is reversed, the tumor drug resistance is overcome, and a better tumor treatment effect is displayed in cooperation with chemotherapy drugs for tumor treatment.

The invention provides a resveratrol-loaded exosome and a preparation method thereof, and belongs to the technical field of biological medicine manufacturing. The preparation method of the resveratrol-loaded exosome comprises the following steps: performing 3D culture on human umbilical cord or hair follicle-derived mesenchymal stem cells under a low-oxygen condition, collecting supernate, and separating by a differential ultracentrifugation method to obtain the exosome of the mesenchymal stem cells; the method comprises the following steps: mixing a resveratrol solution containing a surfactant with the MSCs-Exos, and carrying out co-incubation and ultrafiltration to obtain the resveratrol loaded MSCs-Exos. According to the invention, the surfactant is added, so that not only are the entrapment rate and the drug loading rate of the MSCs-Exos loaded resveratrol greatly improved, but also the activity of the MSCs-Exos is maintained, the common effect of the MSCs-Exos and the resveratrol is fully exerted, and a synergistic effect is achieved. The resveratrol loaded MSCs-Exos disclosed by the invention has an effect of treating diabetes mellitus.

The invention belongs to the technical field of medical materials, and relates to a bioactive glass composition as well as a preparation method and application thereof. The present invention provides a bioactive glass composition, which comprises: a base material, which is a bioactive glass; the surface of the base material is modified by a polyphenol compound. According to the invention, after being subjected to polyphenol compound surface modification, the bioactive glass shows a better sealing effect when being used for dentin exposure sensitive sealing, so that the drug loading rate, the biocompatibility, the water solubility, the biological activity and the antibacterial effect of the bioactive glass are improved; moreover, the acid-resistant stability and the wear-resistant stability of the sealing effect can also be improved when the sealing agent is used for repairing the dentin exposure sensitivity.

The invention discloses a prochloraz ligand metal organic framework material as well as a preparation method and application thereof. The prochloraz ligand metal organic framework material is any one of the following Zn-PRO-1, Zn-PRO-2 and Zn-PRO-3: Zn-PRO-1: the chemical formula of Zn-PRO-1 is [Zn (TPA) (PRO) 2] n, TPA represents terephthalic acid, PRO represents amidino-prochloraz, n represents 1, 2, 3, 4, 5, 6, 7, 8-tetramethyl-1, 3, 4, 5-tetramethyl-1, 3, 4, 5-tetramethyl-1, 3, 4, 5-tetramethyl-1, 3, 4, 5-tetramethyl-1, the chemical formula of Zn-PROO-2 is [Zn (1, 2, 4-BTC) (PRO) 2] n, 1, 2, 4-BTC represents 1, 2, 4-benzene tricarboxylic acid, and PRO represents 2, 3, 4-benzenetricarboxylic acid, and the chemical formula of Zn-PROO-2 is [Zn (1, 2, 4-BTC) (PRO) 2] n, the chemical formula of Zn-PROO-3 is [Zn (NTD) (PRO)] n, NTD represents 1, 4-naphthalenedicarboxylic acid, and PRO represents amidino-prochloraz. Compared with other sustained-release agents, the three materials provided by the invention have higher drug loading rates, all the materials can slowly release prochloraz pesticides, and Zn-PRO-3 has the characteristic of pH response release, has a remarkable antibacterial effect on two plant pathogenic fungi, namely, Colletotrichum gloeosporioides, of strawberry anthracnose pathogenic bacteria and Alternaria alternata of apple black spot pathogenic bacteria, and can be used for preparing the prochloraz sustained-release preparation for preventing and treating the prochloraz pesticides in the prochloraz sustained-release preparation of the prochloraz sustained-release preparation of the prochloraz sustained-release preparation of the prochloraz sustained-release preparation of the prochloraz sustained-release preparation of the prochloraz sustained-release preparation of the prochloraz sustained-release preparation of the prochloraz. A new thought can be provided for the MOF material slow-release bactericide.

The invention relates to the technical field of mineral extraction and compound preparation thereof, in particular to a preparation method and application of a nano compound containing a pan jade extract. The method comprises the following steps: S1, crushing and sieving pan jade to obtain pan jade powder, then adding a first solvent, carrying out ultrasonic treatment, centrifuging, taking supernatant liquid, filtering, and carrying out spin-drying, redissolving and vacuum drying on filtrate to obtain pan jade extract; s2, preparing a ZIF-8 nano material from zinc nitrate hexahydrate and 2-methylimidazole; s3, loading the pan jade extract onto the ZIF-8 nano material, so as to obtain ZIF-8 loaded with the pan jade extract; and S4, carrying out functional modification on the ZIF-8 loaded with the pan jade extract by utilizing folic acid to obtain the nano-composite containing the pan jade extract. The nano-composite containing the pan jade extract prepared by the invention is used for resisting tumor proliferation.

The invention relates to the technical field of nano medicines, in particular to a tanshinol-containing copper-based metal organic framework nano material and a preparation method and application thereof.According to the prepared tanshinol-containing copper-based metal organic framework nano material, tanshinol is dissolved in a dimethylimidazole aqueous solution, copper ions are rapidly added into the solution, and the tanshinol-containing copper-based metal organic framework nano material is obtained after the tanshinol is dissolved in the dimethylimidazole aqueous solution; the tanshinol-loaded copper-based metal organic framework nano-material is prepared from the copper-based metal organic framework nano-material, the tanshinol-loaded copper-based metal organic framework nano-material has high drug loading rate and acid-sensitive drug release performance, and the defects that a small-molecule drug tanshinol is poor in stability in an articular cavity and is easily removed by synovial fluid can be overcome. Compared with a natural enzyme, the nano-enzyme formed by combining tanshinol and copper-based MOF has the active oxygen scavenging capacity, and the treatment effect on osteoarthritis is jointly improved.

The present invention provides an inhalable bionic nanomaterial for treating bacterial pneumonia of the lungs, and a preparation method and application thereof, belonging to the technical field of drug preparation. Mesoporous polydopamine nanoparticles A are prepared by a one-pot synthesis method using block polyether and 3,3',5,5'-tetramethylbenzidine as templates; levofloxacin is added to A, stirred and centrifuged, and freeze-dried to obtain nanoparticles B; RAW264.7 cells are resuspended after centrifugation, homogenized and centrifuged again to collect the supernatant, and the supernatant centrifugal precipitation is the prepared macrophage membrane C; the configured B solution and C are mixed, and a bionic nanomaterial D wrapped in a macrophage membrane coating is prepared by a direct extrusion method. The inhalable bionic nanomaterial prepared by the present invention can cross the complex lung barrier to target the inflammatory site to respond to the release of drugs, and can remove reactive oxygen to relieve lung inflammation, which is of great significance for the treatment of lung infection and has promotion and application value.

The invention relates to the technical field of nano materials, in particular to a preparation method of a Zn-based bimetal MOFs material and application of the Zn-based bimetal MOFs material as a medical drug carrying material. The preparation method comprises the following steps: preparing Zn, Mn and a ligand according to a molar ratio of 1: 1: (72-80), and mainly comprises the steps of preparation of metal salt solutions, coordination polymerization reaction, separation and purification, and drying. The process for preparing the Zn-based bimetallic MOFs material is simple and environmentally friendly, the obtained product has the excellent performance of being small in particle size, regular and stable, and the material loaded with doxorubicin hydrochloride has high loading rate and has potential clinical conversion value.

This invention discloses a liposome, a drug-loaded liposome, its preparation method, and its applications. The liposome comprises a liposome backbone and a targeting ligand; wherein the liposome backbone comprises the following components: phospholipids, cholesterol, cationic lipids, and PEGylated phospholipids; the cationic lipids are (2,3-dioleoyl-propyl)-trimethylammonium chloride and / or 1,2-dioleoyl-3-trimethylammonium propane; and the targeting ligands are sodium hyaluronate and / or hyaluronic acid. This liposome possesses excellent basic properties and excellent targeting ability; after encapsulating the active pharmaceutical ingredients (doxacin hydrochloride and berberine hydrochloride), its targeting ability can be further enhanced in practical applications, thereby increasing its efficacy.

The invention discloses a pH / GSH dual-responsive targeting nanoparticle based on MOF-199, the nanoparticle contains doxorubicin, MOF-199, polydopamine and hyaluronic acid, the doxorubicin is encapsulated in a pore structure of the MOF-199, the surface of the MOF-199 is coated with the polydopamine, and the polydopamine and the hyaluronic acid are grafted through a metal coordination effect. The invention also discloses a preparation method of the nanoparticle, the prepared nanoparticle has tumor targeting property, slow release property and pH / GSH responsive intelligent drug release ability, and also has high biocompatibility and stability, realizes organic combination of chemotherapy / chemical kinetic therapy (CDT) / photo-thermal therapy (PTT), makes up for the deficiency of traditional single therapy, and has broad application prospects. The compound has a great application prospect in preparation of anti-tumor drugs.

The invention relates to the technical field of biological medicine, in particular to bionic naringenin polydopamine nanoparticles and application thereof. Comprising the following steps: dissolving pluronic and dopamine hydrochloride in an ethanol water solution, then adding a 3, 3 ', 5, 5'-tetramethyl benzidine solution, and carrying out ultrasonic treatment for 5-15 minutes; adjusting the pH value to 7.5-8.5, violently stirring at room temperature for 3-5 hours, centrifuging, collecting precipitate to obtain mesoporous polydopamine nanoparticles, and storing at 0-5 DEG C for later use; the mesoporous polydopamine nanoparticles are added into absolute ethyl alcohol to be dissolved, naringenin is added, ultrasonic treatment, stirring and centrifugal collection are conducted on precipitates, and naringenin polydopamine nanoparticles are obtained; carrying out co-incubation on the naringenin polydopamine nanoparticles and the neutrophile granulocytes; therefore, the bionic naringenin polydopamine nano-particles are obtained. The invention further discloses application of the bionic naringenin polydopamine nano-particles in a medicine for treating thoracic aortic aneurysm.

The present invention discloses an application of a dehydrogenated short peptide in the controlled release of anti-tumor drugs, wherein the dehydrogenated short peptide is applied to load chlorambucil to form a dehydrogenated short peptide-loaded chlorambucil gel DHPN1-CLB, thereby realizing the control of the in vitro release of chlorambucil, improving the aggregation of chlorambucil in cells during in vitro release, strengthening the anti-leukemia cell proliferation effect, and inducing cell apoptosis by decreasing the mitochondrial membrane potential of leukemia cells. The results show that the cell uptake experiment shows that the dehydrogenated short peptide-loaded chlorambucil gel DHPN1-CLB has a larger amount of aggregation in cells and is easier to enter cells. DHPN 1 can increase the aggregation of chlorambucil in cells, thereby enhancing the anti-leukemia cell proliferation effect. The chlorambucil-dehydrogenated peptide nanosystem induces cell apoptosis by decreasing the mitochondrial membrane potential of leukemia cells, and is time- and dose-dependent.

The invention relates to a drug loading system, responsive long-acting drug release apatite type bone cement as well as a preparation method and application of the responsive long-acting drug release apatite type bone cement. The drug loading system RES (at) LDH comprises a layered double hydroxide carrier LDH and a drug resveratrol RES loaded on the layered double hydroxide carrier LDH through electrostatic adsorption.

The invention discloses a preparation method of a gold nano zeolite imidazole frame-8 camptothecin derivative preparation, which comprises the following steps: dissolving a chloroauric acid solution in distilled water, and adding sodium citrate to obtain a gold nano solution; adjusting the pH value of the gold nano solution, adding thiohydracrylic acid, stirring, and adding morpholine ethanesulfonic acid to adjust the pH value of the solution; dissolving one of camptothecin, 10-hydroxycamptothecin and irinotecan hydrochloride in water, and adding the solution into the prepared gold nanoparticle solution to obtain a gold nanoparticle-camptothecin derivative solution; dissolving anhydrous zinc acetate and dimethylimidazole in methanol, mixing the anhydrous zinc acetate and the dimethylimidazole, reacting, and centrifuging to obtain zeolite imidazole frame-8 nanoparticles; and completely adding the tannic acid solution into the zeolite imidazole frame-8 nanoparticles, and adding the gold nanoparticle-camptothecin derivative solution to obtain the gold nanoparticle zeolite imidazole frame-8 camptothecin derivative preparation. According to the preparation method of the gold nano zeolite imidazole frame-8 camptothecin derivative preparation, gold nano and zeolite imidazole frame-8 are jointly used as carriers, the drug loading rate of camptothecin derivatives is effectively increased, and the chemotherapy and photo-thermal synergistic treatment effect is effectively improved.

The present invention discloses a preparation method and application of targeted nanovesicles derived from cells. The intracellular nanovesicles of the present invention have a large yield, a small particle size, a narrow particle size distribution range, and have a higher encapsulation rate and drug loading rate when used as a carrier to load drugs. The distribution range and degree are wider under the vitreous cavity injection mode. For example, the loaded drug can be absorbed faster under the vitreous cavity injection form, and has very good application and research value in the medical field. In particular, the present invention gives the intracellular nanovesicles targeting, and obtains targeted intracellular nanovesicles by modifying Lamp2b to carry a targeting peptide. The nanovesicles provided by the present invention have a large yield and strong targeting, can more efficiently play a therapeutic role or a drug carrier role, and have very good application prospects and research value.

The present application provides a kind of aceclofenac enteric microcapsule and its preparation method, the microcapsule includes core and coating, the core includes pill core, aceclofenac, adhesive;The coating includes enteric coating material, plasticizer, anti-adhesive. By screening and optimizing the type and proportion of adhesive and enteric material, optimizing and controlling the preparation process parameters, effectively improve the stability and long-term stability of aceclofenac sustained-release microcapsule, block the release of drug in mouth and stomach, direct the drug to small intestine and slowly release, and the release effect is long-acting and durable.

The invention belongs to the field of pesticides, and particularly relates to a high-permeability glufosinate-ammonium compounded flumioxazin nano suspending agent and a preparation method thereof, and the suspending agent comprises the following components: 5wt%-10wt% of glufosinate-ammonium and flumioxazin, 2.32 wt%-6.78 wt% of a functional additive, a pH regulator, 3wt%-7wt% of a nano carrier and the balance of water. The preparation method comprises the following steps: stirring a compound solution of glufosinate-ammonium and flumioxazin, loading the compound solution to the nano-porous silicon dioxide, mixing with a functional additive solution by taking water as a solvent, adjusting the pH value, and effectively sanding to prepare the nano-suspending agent. Compared with a common suspending agent, the nano suspending agent has high permeability, the preparation method is simple and efficient, the particle size is as small as nanoscale, the nano suspending agent has high biocompatibility and bioavailability, and druggability, preparation stability, biological activity and the like of pesticide compounds can be improved to a great extent. The lasting action time of the pesticide and the permeability and the adhesiveness of the pesticide on the leaf surfaces of plants are enhanced, so that the use amount of the pesticide is reduced.

The present invention discloses a black phosphorus-methylene blue nanocomposition for precise targeted treatment of Tau phosphorylation and a synthesis method thereof, wherein the synthesis comprises the following steps: step 1, mixing black phosphorus nanosheets with polyethylene glycol for reaction; step 2, centrifuging and cleaning the reaction solution in step 1, and collecting the lower layer reactant; step 3, adding amination superparamagnetic nanoparticles and methylene blue to the lower layer reactant obtained in step 2 for reaction; step 4, centrifuging and cleaning the reaction solution obtained in step 3, and collecting the lower layer reactant. The present invention also discloses a black phosphorus-methylene blue nanocomposition synthesized by the above-mentioned synthesis method, wherein the drug loading rate of methylene blue in the composition exceeds 60%, the composition has precise targeted treatment capability, good biocompatibility, is nontoxic to cells, and the manufacturing process is very simple and convenient, and has broad application prospects in the treatment of Alzheimer's disease.

The invention belongs to the field of preparation of plant-derived functional materials, and particularly relates to puerarin-pueraria starch-Span80 gel beads as well as a preparation method and application of the puerarin-pueraria starch-Span80 gel beads. The method is realized by the following steps: firstly, weighing pueraria starch, and dispersing the pueraria starch in ultrapure water containing sodium alginate to obtain a gelatinized pueraria starch solution; adding a surfactant into the gelatinized pueraria starch solution, stirring, dropwise adding the puerarin-ethanol solution, and continuously stirring after dropwise adding; and sucking the supernate, dripping into a calcium lactate solution, curing into balls, and performing vacuum freeze drying to obtain the product. The puerarin-embedded gel bead prepared by the invention has pores on the surface, and the structure is of a porous three-dimensional network structure, so that the adsorption and embedding of subsequent active substances are facilitated; a texture result shows that in addition to elasticity, the hardness, cohesiveness, gelatinization, chewiness and resilience of the gel beads before freeze-drying are as follows: puerarin-pueraria starch-Span80 gel beads > puerarin-pueraria starch gel beads > pueraria starch gel beads; and the swelling property is good and can reach 6.94 + / -6.34%.

The invention discloses a biodegradable two-sided ring brush-shaped polymer and a preparation method thereof.The two-sided ring brush-shaped polymer has a structure shown in the formula I. An annular inner core is a biodegradable macromolecule PCL, an annular outer core is a POEGMA chain with good biocompatibility and serves as a hydrophilic chain segment, and the annular two-sided ring brush-shaped polymer is formed; the polymer can be self-assembled in an aqueous solution to form a stable nano-micelle with a hydrophilic shell and a hydrophobic core, and the nano-micelle is high in drug loading rate and high in encapsulation efficiency. # imgabs0 #

The invention belongs to the technical field of extraction and utilization of collagen, and particularly relates to an extraction process of a jellyfish extract and a preparation process of a drug-loaded collagen microneedle preparation. The extraction process of the jellyfish extract comprises the following steps: cutting jellyfish into strips, removing salt, removing impure protein, removing fat, removing pigment, homogenizing, continuing enzymolysis, salting out, dialyzing and drying to obtain the enzyme-soluble collagen. The preparation process of the drug-loaded collagen microneedle preparation comprises the following steps: dissolving soluble collagen in water, preparing precipitate after centrifugation into a jellyfish collagen gel solution, mixing the jellyfish collagen gel solution with a drug solution, and adding the mixture into a microneedle mold to obtain the drug-loaded collagen microneedle. The extraction process of the jellyfish extract, provided by the invention, is low in extraction cost, simple to operate and high in extraction rate, and the extracted enzyme-soluble collagen is high in purity; the extracted enzyme-soluble collagen is used for preparing a drug-loaded collagen microneedle preparation, and has the advantages of good biocompatibility and strong drug-loading capability.

The invention relates to a preparation method of goserelin microspheres and a product thereof, and the preparation method comprises the following steps: mixing a goserelin raw material solution and a degradable polymer solution to obtain an oil phase; mixing a surfactant with water to obtain a water phase; mixing the oil phase with the water phase to form an emulsion, stirring and curing to obtain goserelin microspheres; wherein solvents of the goserelin raw material solution and the degradable polymer solution are respectively and independently cosolvents, and the cosolvents are obtained by mixing a first solvent and a second solvent. Compared with a traditional multiple emulsion method, the preparation method is simpler to operate, meanwhile, the prepared goserelin microspheres have higher drug loading rate and smaller particle size, injection administration of a No.7 needle can be achieved, the surfaces of the microspheres are free of holes, burst release is low, the drugs can be evenly distributed in the microspheres, and acetic acid residues are basically avoided.