31 results about "Dermal sensitization" patented technology

The invention provides an ultrasound gel. The ultrasound gel contains the following components in percentage by weight: 0.1 to 10 percent of glycosaminoglycan, and 5 to 15 percent of glycerol. The ultrasound gel has the characteristics of high-efficiency disinfection and sterilization, no cytotoxicity, no skin allergy and no irritation.

The invention discloses a swelling-type hollow silk fibroin micro-needle drug delivery system and a preparation method thereof. According to the technical scheme of the preparation method, a swelling modified silk fibroin solution is casted in a PDMS mold to be vacuumized, dried and molded as a hollow needle-type housing. After that, a silk fibroin micro-needle array provided with cavities in the hollow needle-type housing is filled with powder-like or solution-like drugs, vacuumizing and drying are carried out to form a drug part. Finally, a layer of modified silk fibroin solution is cast, dried and molded as a cladded coating. The cladded coating is removed to obtain the swelling-type hollow silk fibroin micro-needle drug delivery system. The drugs are wrapped inside the cavities of the silk fibroin micro-needle arrays, so that the system is high in drug loading ratio and low in skin allergenicity and stimulation. The system facilitates the sustained release and the controlled release of micro-needle drugs. Meanwhile, according to the preparation method, since the swelling modified silk fibroin solution is cast, dried and molded in a mild processing condition, the system is low in processing cost and suitable for large-scale production. Drugs are directly wrapped in the cavities of the swelling hollow micro-needles, so that controlled-release drugs are swelled in the micro-needle base material. In this way, the biological activities of the controlled-release drugs are maintained, while the structure of the hollow silk fibroin micro-needle drug delivery system is greatly simplified. The system is better in practicality.

The invention relates to a preparation method of silver ion hydrogel dressing. The method comprises the following steps: firstly, a hydrophilic high-molecular polymer aqueous solution is prepared, and is subjected to vacuum defoamation for standby; secondly, the hydrophilic high-molecular polymer aqueous solution obtained in the first step is prepared into a hydrogel material semi-finished product through a coating method or an injection molding method; thirdly, the hydrogel material semi-finished product is prepared into hydrogel material through the radiation of high energy ray; fourthly, the hydrogel material is dipped into silver salt aqueous solution, so that the hydrogel material absorbs silver ion loaded in the silver salt aqueous solution, and then the silver ion hydrogel dressing is obtained. The prepare silver ion hydrogel dressing prepared through the method has good biocompatibility, is non-irritative and non-sensitized to skin, provides wet healing environment for wound healing, promotes wound healing, moreover can slowly release silver ion, effectively restrains and prevents the growth of common bacteria on the wound, and can be applied to nursing of various acute and chronic wounds.

The invention provides a dog-skin plaster and a preparation technique thereof. The dog-skin plaster is made from a substrate and raw herbal materials of black-plaster dog-skin plasters. The mass ratio of the raw herbal materials is the same as the mass ratio in the prescription of the black-plaster dog-skin plaster. A processed radix aconiti replaces an unprocessed radix aconiti, and a processed kusnezoff monkshood root replaces an unprocessed kusnezoff monkshood root, wherein no lead is contained. The preparation uses an ethanol extracting technology and a volatile-oil extracting technique to replace the extracting technology of frying vegetable oil in the original preparation, which can completely extract drug ingredients, without such disadvantages as drug ingredients being easily destroyed by high temperature and air pollution which are generated by the extracting technique of frying vegetable in the original preparation; the new substrate replaces the original black-plaster substrate, which has the advantages of larger drug quantity, easy putting up and off, good compatibility, release property and skin penetration property, without toxic lead content. The unprocessed radix aconiti and the unprocessed kusnezoff monkshood root in the original prescription are replaced respectively by the processed radix aconiti and the processed kusnezoff monkshood root, thus ensuring the effectiveness and safety of the preparation and eliminating the skin sensitization caused by the original dog-skin plaster.

The invention discloses a medical ultrasonic coupling agent and a preparation method thereof and belongs to the field of traditional Chinese medicines. The medical ultrasonic coupling agent disclosed by the invention is prepared from the following raw materials of effective components: yellow soybeans, hibiscus tiliaceus, persicaria hydropiper, lysimachia christinae, fruits of few flower wildrice, smallfruit fig aerial roots, sambucus chinensis, dwarf elm fruits, ligustrum leaves, buddleia, sundew, hibiscus syriacus, iris-leaf oberonia herb, mint, cimicifugae foetidae and giant soft-shelled turtle fat. The medical ultrasonic coupling agent disclosed by the invention is prepared from natural Chinese herbal medicines in compatibility, has the unique curative effect of lubricating skins and promoting circulation and removing stasis, has the characteristics of efficient disinfection and sterilization, no cytotoxicity, no cutaneous sensitization and no irritation and can be used for avoiding cross infection of clinically ultrasonic inspection. The medical ultrasonic coupling agent disclosed by the invention for ultrasonic inspection is more remarkable in acoustic transmission effect and clearer in ultrasonic imaging.

The invention belongs to the technical field of cosmetics, and particularly relates to a moisturizing and skin-refreshing mask and a preparation method thereof. The mask comprises the following components in parts by weight: cassava gelatinized starch, soybean protein, carbomer, aloe extract, seaweed extract, chamomile extract, hydroxyethyl cellulose, glycerol, calcium hydroxide, preservative composition and deionized water. The moisturizing and skin-refreshing mask provided by the invention is prepared from the food-grade cassava gelatinized starch, and is high in safety, soft in texture andgood in skin application property; the aloe extract, the seaweed extract and the like are added as moisturizing components, so that the mask is endowed with an excellent moisturizing effect, the skinis smoother and more elastic after the mask is used, and fine wrinkles are reduced; the preservative composition composed of pistachio extract, phellinus linteus extract and isoferulic acid is added into the mask, so that the mask is endowed with a good anti-corrosion effect, and the anti-corrosion components are all natural plant components and low in allergenicity on skin, and remarkably prolongs the shelf life of the mask.

The invention discloses an ultrasonic B coupling agent and a preparation method thereof. The ultrasonic B coupling agent consists of the following components in parts by weight: radix bupleuri, herbalobeliae chinensis, honeysuckle, wild chrysanthemum flos, astragalus membranaceus, mint, folium artemisiae argyi, fructus forsythia, radix sophorae flavescentis, dioscorea bulbifera, aloe, white poria, angelica sinensis, xanthan gum, sodium carboxymethyl cellulose, alcohol and 87.6-93% of deionized water. The ultrasonic B coupling agent disclosed by the invention has the characteristics of being efficient in sterilization and bacterium killing, safe and non-toxic, free of skin sensation and free of irritation, is capable of preventing cross infection of clinical ultrasonic inspection, is capable of effectively killing various bacteria, and the like, has broad spectrum properties and is remarkable in sterilization effect, and in addition, the ultrasonic B coupling agent is novel in scheme,low in cost and simple to prepare.

The invention discloses a composition for treating hyperostosis and joint arthralgia spasm pain as well as a preparation method of the composition. The composition is prepared from the following components in parts by weight: 20 to 40 parts of red smilax glabr, 20 to 40 parts of flos carthami, 20 to 40 parts of caulis sinomenii, 20 to 40 parts of pyrrosia leaf, 20 to 40 parts of root of wintersweet, 20 to 40 parts of spinyleaf pricklyash root, 2 to 4 parts of rhizoma arisaematis, 2 to 4 parts of rhizoma pinelliae, 2 to 4 parts of radix aconiti agrestis, 4 to 6 parts of sangusis draconis and 4to 6 parts of sea horse, 40 to 60 parts of fresh ramulus mori, 40 to 60 parts of fresh peach twig, 40 to 60 parts of fresh ulmus pumila, 40 to 60 parts of fresh willow twig, 40 to 60 parts of Japanesepagodatree juvenile branchlet, 350 to 520 parts of red lead and pharmaceutically effective adjuvants. According to the preparation method of the composition, disclosed by the invention, an extractionprocess, a matrix and a forming process of an original preparation are changed, so that the conditions that pharmaceutical ingredients are easily destroyed at high temperature, produce air pollutionand the like in the original process are avoided. An original black paste dosage form is replaced with a new dosage form; the new dosage form has the advantages of definite curative effects, high safety, easiness in uncovering and pasting, no pollution to clothes, no poisonous lead elements or skin sensitizing property in the original preparation and no pollution to the environment; huge economicbenefits can be obtained when the composition for treating the hyperostosis and the joint arthralgia spasm pain is put into production.

The invention belongs to the field of medicine, and relates to a medical ultrasonic coupling agent directed at defects in the prior art. The medical ultrasonic coupling agent is prepared from components as follows: Chinese angelica, radix saposhnikoviae, glossy privet leaves, honeysuckle stems, mint, waxgourd seeds and girald daphne barks; the selected medicinal materials of the medical ultrasonic coupling agent are compatible and fit, so that the medical ultrasonic coupling agent has a better coupling effect and characteristics of efficient sterilization and disinfection and no cytotoxicity, skin sensitization property or irritation, and is capable of preventing cross infection; with the adoption of the medical ultrasonic coupling agent for ultrasonic examination, the sound transmission effect is more remarkable, and ultrasonic images are more clear.

The invention discloses an ultrasonic coupling agent for color ultrasonography and a preparation method of the ultrasonic coupling agent, belonging to the field of traditional Chinese medicines. The ultrasonic coupling agent for color ultrasonography is prepared from the following active ingredients: cherries, leaves of Acronychia pedunculata (L.) Miq., tuber fleeceflower stem, pine needles, scabrous mosla herb, folium eucalypti, seeds of Pinus koraiensis Sieb. et Zucc., common achyranthes herb, leaf of shrubby baeckea, platycladi seeds, mint, seeds of Chinese waxgourd, lotus seed pots, entireleaf neoalsomitra, rubus ellipticus smit and cow's milk. According to the ultrasonic coupling agent for color ultrasonography, the natural traditional Chinese medicinal materials are mixed for use, the ultrasonic coupling agent has the characteristics of realizing efficient disinfection and sterilization, and being free of cytotoxicity, cutaneous sensitization and irritation, and can achieve the unique curative effects of lubricating the skin, as well as promoting blood circulation to remove blood stasis, the cross infection of the ultrasonic testing in clinic can be avoided, the acoustic transmission effect is obvious, the ultrasonic imaging is clear, and the ultrasonic coupling agent can be widely applied to the clinical color ultrasonography checking.

The invention belongs to the technical field of cosmetics, and particularly relates to a whitening and spot-fading mask and a preparation method thereof. The mask takes tricholoma matsutake extract, acacia farnesiana extract, pentapeptide, rehmannia extract and dipotassium glycyrrhizinate composite watercress extract as functional components, can effectively solve the problems of dark and gloomy skin, color spots and the like, and makes the skin moisturized and whiter; and meanwhile, a preservative composition consisting of pistachio extract, phellinus linteus extract and isoferulic acid is added into the mask disclosed by the invention. The preservative composition has a remarkable inhibition effect on various common contaminating bacteria in cosmetics, the effective components are all natural plant components, the skin sensitization is low, the pH capacity limit value is wide (3-9), and the shelf life of the mask is remarkably prolonged.

The invention relates to a Chinese medical emplastrum substrate and an external use emplastrum prepared by the substrate, which comprises the materials in weight proportion as follows: paraffin 5% to 40%, monoglyceride 5% to 20%, glycerol 1% to 10%, swelling gelatin 2% to 20%, sodium polyacrylate/glycerol solution 5% to 15%, or neutralization 70% sodium polyacrylate liquid 5% to 20%, or polyacrylic resin 5% to 15%, sodium lauryl sulfate 0.5% to 2%, stearic acid 0 to 50%, ethanolamine 0 to 20%. The substrate is suitable for the external use emplastrum which is made of the drug efficacy material powder, the water soluble extract of a drug efficacy material or a volatility component of extraction combined with a backing composite material. The added raw powder amount can reach to 25 wt%; the added extract amount can reach to 40 wt%. The Chinese medical emplastrum substrate has the advantages of good compatibility, large drug loading, good skin stickness, easy uncovering, easy sticking, no skin allergy, good steady, original shape maintaining and no melting in 50 DEG C, good discharging, good skin permeability, easy cleaning and no skin stickness.

A cell model for in vitro evaluation of compound-induced skin sensitization and a constructing method therefor. The method for constructing the cell model comprises the steps of: designing and constructing an sgRNA expression vector based on CRISPR/Cas9 vector system; designing and constructing a homologous recombinant vector capable of knocking a reporter gene linked to a self-cleaving peptide sequence into a specific site of the expression frame of the HMOX1 gene; co-transfecting the homologous recombinant vector, an hCas9 plasmid and the sgRNA expression vector into a cell, and carrying out monoclonal expansion to obtain the cell model. The present invention obtains a HaCaT cell model in which a luciferase gene is knocked in before the stop codon of the HMOX1 gene by combination of CRISPR/CAS9 and a monoclonal cell technique. The cell model realizes synchronous expression of the luciferase gene and the HMOX1 gene, thereby effectively distinguishing sensitizing compounds from non-sensitizing compounds.

The invention discloses a method for in-vitro high-flux screening of skin allergens based on a fluorescent chemical reaction system. The method comprises the steps of: (1) preparation of the fluorescent chemical reaction system; (2) allergen screening test; and (3) skin allergenicity prediction. The method is quick, simple, convenient, low in cost, high in standardization degree and wide in application range, and can be used for quickly and effectively detecting and evaluating chemicals, cosmetics, plant extracts, cosmetic raw materials and the like.

The invention discloses compound essential oil for treating RFM (Retained Fetal Membranes). The compound essential oil is prepared from the following plant essential oil in parts by volume: 1 to 5 parts of jasmine oil, 1 to 5 parts of thyme oil, 1 to 5 parts of lavender oil and 200 to 400 parts of sweet almond oil. The invention also provides a preparation method of the compound essential oil. The compound essential oil for treating the RFM and the preparation method thereof, disclosed by the invention, have the beneficial effects that adopted essential oil is extracted and purified from flowers, leaves, stems or fruits of plants, is natural and cannot generate drug resistance, tests on skin irritation, skin sensitization and uterine toxicity show that the compound essential oil is safe in clinical use and has no toxic or side effect, a clinic test shows that the effective rate of treating the RFM by using the compound essential oil is up to 72.73 percent, and the treatment effect is remarkable.

The invention relates to the field of traditional Chinese medicine application, and particularly discloses a medical bactericidal antiallergic ultrasonic coupling agent. The ultrasonic coupling agent is prepared by taking sweet wormwood, halite, folium isatidis, garden euphorbia herb, devilpepper, rhizoma anemarrhenae, sarcandra glabra, rehmannia, aloe and tea begonia as raw materials and using a conventional preparation method to obtain a gel preparation. The medical bactericidal antiallergic ultrasonic coupling agent is simple to prepare, is prepared by selecting and combining natural Chinese herbal medicines, thereby having unique curative effect on lubricating skin, sterilizing and relieving itch, and has the advantages of being bactericidal and free of cytotoxicity, skin sensitivity and irritation, and allergic reaction of a patient during clinical ultrasonic examination is avoided.

The invention relates to the technical field of sensitization testing, in particular to a method for testing skin sensitization in a human cell line activation test. A method for testing skin allergenicity by a human cell line activation test comprises the following steps: step S1, cell culture and cell stability detection: THP-1 suspension cells are cultured in an incubator by using a 1640 complete medium under the cell culture conditions of 37 DEG C and 5% CO2, the cell state is observed, and the cells are recovered and are used for testing after passing cell stability detection; and S2, confirming the concentration of CV75: culturing the cells in a cell culture box for 48 hours at the density of 2 * 10 < 5 > cells/mL, and centrifuging. The human cell line activation test method for skin sensitization is an in-vitro detection method with high stability and reliability, can be applied to detection of skin sensitization substances, and solves the problems that a traditional skin sensitization detection method is low in reliability and safety and needs animal experiments for detection.

The invention belongs to the technical field of cosmetics, and particularly relates to an antiseptic composition for cosmetics and a preparation method for the antiseptic composition. The antiseptic composition comprises the following components in parts by weight: 1-10 parts of fructus pistaciae verae extract, 1-10 parts of phellinus linteus extract, 1-8 parts isoferulic acid, 3-20 parts of polyol and 40-60 parts of deionized water. the antiseptic composition provided by the invention consists of the fructus pistaciae verae extract, the phellinus linteus extract and the isoferulic acid; the antiseptic composition has a significant inhibitory effect on various common contaminating bacteria in the cosmetics, and can broaden the antibacterial spectrum of a single component; the active ingredients are all natural ingredients, so that the antiseptic composition has low skin sensitization, and has a broad range of pH tolerance value (3-9); and the antiseptic composition can be applied to various skin care products, color cosmetics or cosmeceuticals.

A curable liquid composition includes a monomer having an SI value of less than 3.0 and a photopolymerization initiator having a weight average molecular weight of 500 or greater, wherein the curableliquid composition is negative in skin sensitization.

The invention relates to a method for evaluating the safety of substances in vitro, and discloses a method which is more stable, reproducible, economical and easy to operate by using human immortalized myeloid cells. The invention relates to a method for evaluating skin sensitization and/or fever of a substance to be detected by using human immortalized myeloid cells, a method for detecting skin sensitization substances and/or fever substances in a sample, and a method for evaluating the effect of the sample on the function of immune cells. The method comprises the step of measuring the yield of IL-6 and/or IL-8 in a culture solution of human immortalized myeloid cells.

The invention discloses a B-ultrasonic coupling agent and a preparation method, and belongs to the field of traditional Chinese medicines. The B mode ultrasonic coupling agent is prepared from the following raw materials with effective ingredients of cherry, sansevieria, rubus parvifolius, leaf of purpleflower holly, baeckea frutescens, zornia diphylla, eucalyptus robusta, garlic, piper betle, polygonum hydropiper, leaves of mussaenda pubescens, agastache rugosus, leaves of camphor trees, dried orange peel, plum kernel and costustoot. The B mode ultrasonic coupling agent has the characteristics that the selected natural herbal Chinese medicines are compounded, and the particular effects of lubricating skin, activating blood circulation and removing blood stasis, inhibiting bacteria and diminishing inflammation, preventing corrosion and relieving itching, and regenerating tissue and closing sores are realized; the disinfection and sterilization are efficient, the safety and non-toxic effect are realized, the skin allergy and irritation are avoided, and the crossing infection of ultrasonic examination in clinics is avoided; the cost is low, and the preparation is simple; when the B mode ultrasonic coupling agent is used for ultrasonic examination, the acoustical transmission effect is more obvious, and the ultrasonic imaging is clearer.

The invention discloses a probiotic skin care product for reducing skin allergy and a preparation method thereof. The probiotic skin care product uses active probiotic lyophilized powder as an active raw material, and the probiotic in the active probiotic lyophilized powder is Lactobacillus rhamnosus and Lactobacillus delbrueckii, the quantity ratio of said Lactobacillus rhamnosus and Lactobacillus delbrueckii is preferably 0.5:1-2:1. The probiotic skin care product can be in the form of an active moisturizing spray, preferably including the following components by mass fraction: 2-10% active probiotic freeze-dried powder, recombinant human epidermal growth factor 2000IU/mL, and the rest being physiological saline. The preparation method of the present invention is simple and easy to popularize, and can be used for whole body skin moisturizing and maintenance including facial care, and can effectively inhibit skin allergy or inflammatory response, reduce skin sensitization, enhance skin resistance to bacterial infection, and promote skin barrier repair.