Preparation of porous high-swelling gelatin microspheres

A technology of gelatin and microspheres, which is applied in the direction of non-active ingredients of polymer compounds, medical science, bulk transportation, etc., can solve the problems of slow drug loading and low drug loading rate, achieve the conditions, enhance the application effect, and improve The effect of drug loading

Active Publication Date: 2014-12-03
JIANGSU GOLDEN AUTUMN CORD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aiming at the current shortcomings of low drug loading rate and slow drug loading speed of gelatin microspheres, the solution of the present invention is to prepare gelatin microspheres by means of adding porogen and suspending cross-linking

Method used

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  • Preparation of porous high-swelling gelatin microspheres
  • Preparation of porous high-swelling gelatin microspheres
  • Preparation of porous high-swelling gelatin microspheres

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Put 50 ml of paraffin oil in a 250 ml three-neck flask, add 0.4 ml of Span-80 and 0.2 ml of Tween-60, and stir at room temperature for 10 minutes. In addition, in a 100 ml beaker, prepare 10 ml of 30% gelatin aqueous solution with distilled water, then add 1.5 grams of nano-calcium carbonate powder and mix well; pour this solution into the previously mixed paraffin oil and Span-80 and Tween- 60 dispersion, continue to stir for 1 hour, add 1M NaOH solution dropwise to adjust the pH to 8-9, slowly add 2.5 ml of 36% formaldehyde solution dropwise, react for 2 hours, filter and separate the microspheres, soak the microspheres with 15 ml of 5% acetic acid solution Stir the suspension for half an hour, filter and separate the microspheres, and repeat this cycle three times to completely dissolve and remove the calcium carbonate, filter under reduced pressure, and dry in vacuum for 48 hours to obtain light yellow gelatin microspheres.

Embodiment 2

[0019] With embodiment 1, but the weight that adds nano-calcium carbonate powder is respectively 1 gram, 2.0 gram, 2.7 gram and reacts, removes porogen again, purifies and dries.

Embodiment 3

[0021] Put 50 ml of paraffin oil in a 250 ml three-neck flask, add 0.4 ml of Span-80 and 0.2 ml of Tween-60, and stir at room temperature for 10 minutes. In addition, in a 100 ml beaker, prepare 10 ml of 30% gelatin aqueous solution with distilled water, then add 4.5 grams of PEG200 liquid and mix well; pour this solution into the well-mixed paraffin oil and Span-80 and Tween-60 Continue to stir for 1 hour, add 1M NaOH solution dropwise to adjust the pH to 8-9, slowly add 2.5 ml of 36% formaldehyde solution dropwise, react for 2 hours, filter and separate the microspheres, soak and stir the microspheres in deionized water to suspend After half an hour, the microspheres were separated by filtration, and the cyclic operation was repeated three times. The PEG200 was completely dissolved and removed, filtered under reduced pressure, and vacuum-dried for 48 hours to obtain light yellow gelatin microspheres.

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Abstract

The invention discloses a preparation method of porous high-swelling gelatin microspheres. The preparation method comprises the following steps: preparing gelatin into a water solution with the mass concentration of 30%, adding another component as a pore-foaming agent, wherein the pore-foaming agent can be an inorganic substance such as nano calcium carbonate powder as well as a water-soluble high polymer such as polyethylene glycol, polyvinyl alcohol, polymethylacrylic acid-2-(N,N-dimethyl) amino ethyl ester, uniformly mixing the water solution of gelatin with the pore-foaming agent, suspending and dispersing in paroline, adding a spann-80 and tween-60 mixed stabilizer, slowly dropwise adding a formaldehyde solution while stirring, reacting for hours so that the gelatin is crosslinked; then, filtering and separating microspheres, removing the pore-foaming agent, purifying and drying, thus obtaining the porous high-swelling gelatin microspheres. When the microspheres are used as a medicine-carrying embolic agent, the medicine carrying rate and the medicine carrying speed of medicines can be increased, and the use performance is improved.

Description

technical field [0001] The invention relates to a method for preparing porous and highly swellable gelatin microspheres, belonging to the technical field of biological materials and sustained release. Background technique [0002] Ordinary chemotherapy methods have relatively large toxic and side effects for the treatment of malignant tumors, so interventional therapy has become the preferred method for the treatment of advanced malignant tumors. For example, embolization microspheres can be used as a drug dosage form for the treatment of inoperable liver cancer in the middle and advanced stages. Through selective arterial cannulation, the microspheres are injected into the target tissue, and the blood supply artery of the tumor is blocked, so as to achieve the purpose of treating liver cancer. [0003] Polymer embolization microspheres have a wide range of applications in the medical field. At present, there are many studies on blank embolization microspheres at home and ...

Claims

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Application Information

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IPC IPC(8): C08J9/28C08J3/24C08L89/00A61K9/16A61K47/42A61L31/04A61L31/16
Inventor 倪才华张丽萍石刚王佳敏
Owner JIANGSU GOLDEN AUTUMN CORD CO LTD
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