Sericin-based semi-interpenetrating temperature-sensitive nano-composite hydro-gel and preparation method thereof

A nanocomposite and semi-interpenetrating technology, applied in the field of sericin-based semi-interpenetrating temperature-sensitive nanocomposite hydrogel and its preparation, can solve problems such as insufficient biocompatibility, achieve excellent cell compatibility, and prepare methods Simple, source-rich effects

Inactive Publication Date: 2013-04-10
TIANJIN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to design a sericin-based semi-interpenetrating temperature-sensitive nanocomposite hydrogel, so that the gel has high transparency, high swelling degree, rapid temperature response and excellent cell compatibility, especially to overcome the current Insufficient biocompatibility of nanocomposite hydrogels

Method used

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  • Sericin-based semi-interpenetrating temperature-sensitive nano-composite hydro-gel and preparation method thereof
  • Sericin-based semi-interpenetrating temperature-sensitive nano-composite hydro-gel and preparation method thereof

Examples

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Effect test

Embodiment 1

[0035] 1) Dispersion of inorganic nano-saponite particles: at 20°C, add hectorite to deionized water to prepare an aqueous solution with a concentration of 1% by mass, and stir for 25 minutes until completely dispersed to form a transparent solution;

[0036] 2) Preparation of the reaction pre-polymerization liquid: under the protection of nitrogen, add sericin and isopropylacrylamide to the above-mentioned transparent solution in sequence, and the concentration of sericin and alkylacrylamide monomers in the aqueous solution is 1% by mass respectively and 9%, stirred at 20°C for 25 minutes to form a slightly pale yellow reaction prepolymer solution;

[0037] 3) Preparation of gel: Cool the above reaction prepolymerization solution to 2°C with ice cubes, add an aqueous initiator solution with a mass percentage concentration of 0.1% under nitrogen protection and magnetic stirring, stir for 15 minutes, and inject a mass percentage concentration of 0.1% % catalyst aqueous solution...

Embodiment 2

[0041] 1) Dispersion of inorganic nano-saponite particles: at 22°C, lithium magnesium silicate was added to deionized water to prepare an aqueous solution with a concentration of 1.5% by mass, and stirred for 25 minutes until completely dispersed to form a transparent solution;

[0042] 2) Preparation of the reaction pre-polymerization solution: under the protection of nitrogen, add sericin and isopropylacrylamide to the above-mentioned transparent solution in sequence, and the mass percent concentration of sericin and alkylacrylamide monomers in the aqueous solution is 2% respectively and 8%, stirred at 22°C for 30 minutes to form a slightly pale yellow reaction prepolymer solution;

[0043] 3) Preparation of gel: Cool the above reaction prepolymerization solution to 1° C. with ice cubes, add an aqueous initiator solution with a mass percentage concentration of 0.15% under nitrogen protection and magnetic stirring, stir for 20 minutes, and inject a mass percentage concentratio...

Embodiment 3

[0047] 1) Dispersion of inorganic nano-saponite particles: at 25°C, add hectorite to deionized water to prepare an aqueous solution with a concentration of 3% by mass, and stir for 30 minutes until completely dispersed to form a transparent solution;

[0048] 2) Preparation of the reaction pre-polymerization solution: under the protection of nitrogen, add sericin and isopropylacrylamide to the above-mentioned transparent solution in sequence, and the mass percent concentrations of sericin and alkylacrylamide monomers in the aqueous solution are 3% respectively and 7%, stirred at 25°C for 35 minutes to form a slightly pale yellow reaction prepolymer solution;

[0049] 3) Preparation of the gel: Cool the above reaction pre-polymerization liquid to 1°C with ice cubes, add an aqueous initiator solution with a mass percentage concentration of 0.1% under nitrogen protection and magnetic stirring, stir for 15 minutes, and inject a mass percentage concentration of 0.2 % catalyst aqueo...

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Abstract

The invention relates to a sericin-based semi-interpenetrating temperature-sensitive nano-composite hydro-gel and a preparation method thereof. The hydro-gel comprises a sericin polypeptide chain part, an inorganic nano-soapstone particle part and a polymer formed after an alkylacrylamide monomer is polymerized. In the hydro-gel, a semi-interpenetrating network technology is mainly adopted, and by combining the physical crosslinking effect of inorganic nano-soapstone particles, the sericin, the inorganic nano-soapstone particles and in-situ free radical of the alkylacrylamide monomer are polymerized. The preparation method comprises the following steps of: dispersing the inorganic nano-soapstone particles; preparing a reaction prepolymerization solution; preparing gel; and purifying the gel. The sericin-based semi-interpenetrating temperature-sensitive nano-composite hydro-gel has rich raw material sources, simple preparation method, low cost, high swelling degree and excellent cell compatibility, can be adjusted according to the content of sericin, and has a wide application prospect in artificial skin, artificial cartilage, cell culture stent, bioreactor and other biomedical aspects.

Description

technical field [0001] The invention relates to the fields of biological materials and intelligent polymer materials, in particular to a sericin-based semi-interpenetrating temperature-sensitive nano-composite hydrogel and a preparation method thereof, which can be applied to technical fields such as biomedicine, tissue engineering, and drug release. Background technique [0002] Silk is composed of silk fibroin and sericin. Sericin wraps around the silk fibroin and accounts for about 25% of the mass of the cocoon layer. It is mainly used to glue and protect the silk fibroin. In addition, it also contains a small amount of wax, pigment, and carbohydrates. and inorganic components. Sericin (SS) is a globular protein with a relative molecular mass of 14,000 to 314,000 and composed of 18 amino acids. Polar side chain amino acids account for 74.61% of the amino acid composition of sericin, so sericin has good water absorption, water solubility, anti-oxidation, promotion of cell...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/075C08L33/24C08L89/00C08K3/34C08F120/54C08F2/44
Inventor 张青松李学伟陈莉郭冠群赵义平
Owner TIANJIN POLYTECHNIC UNIV
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