63results about How to "Active targeting" patented technology

The invention discloses a magnetic resonance imaging nanometer drug carrier, and a nanometer drug loading system and a preparation method thereof. The magnetic resonance imaging nanometer drug carrieris a triblock polymer nanoparticle, wherein the triblock polymer is PLGA-PEI-PEG, and has active groups on the surface, and the active groups comprise amino, hydroxyl and carboxyl. According to the present invention, the drug carrier can efficiently load a nuclear magnetic resonance imaging drug and an antitumor drug to make the antitumor drug specifically reach the tumor lesion site, such that the nuclear magnetic resonance positioning of the superparamagnetic ferroferric oxide nanoparticle at the tumor region can be achieved while the high-selectivity and low-toxicity treatment effect can be achieved, and the disadvantages of poor selectivity, strong toxic-side effect, easy drug-resistance generation and the like of the traditional cytotoxic drugs can be overcome; and the preparation method is simple and is easy to perform, the prepared triblock polymer nanoparticle can be stably stored in the aqueous solution so as to be easily stored, and various functional groups exist on the surface of the particle, such that the prepared triblock polymer nanoparticle can be easily subjected to surface modification or surface functionalization.

The invention discloses a photosensitizer phospholipid compound, a preparation method of the photosensitizer phospholipid compound, a pharmaceutical composition and application of the photosensitizer phospholipid compound. The pharmaceutical composition is the photosensitizer phospholipid or a combined pharmaceutical composition of the photosensitizer phospholipid and a pharmacologically acceptable carrier, and the pharmaceutical composition is in the form of a liquid preparation, a solid preparation, a semisolid preparation, a capsule, granules, a gel or an injection. The photosensitizer phospholipid compound or a liposome of the photosensitizer phospholipid is applicable to the photodynamic therapy of tumors; the photosensitizer phospholipid compound or the liposome of the photosensitizer phospholipid compound can be rapidly taken by cells and can release a photosensitizer crude drug, and under laser action, the photosensitizer phospholipid compound or the liposome of the photosensitizer phospholipid compound can rapidly release active oxygen to take a strong anti-tumor effect. The photosensitizer phospholipid compound and a liposome nanoparticle thereof can serve as a liquid preparation, a solid preparation, a semisolid preparation, a sterilized preparation and a sterile preparation; and the photosensitizer phospholipid compound and the liposome nanoparticle are low in toxicity and are applicable to the efficient treatment of various tumors.

The invention provides modified polyethyleneimine, and a preparing method and applications of the modified polyethyleneimine. The modified polyethyleneimine is prepared through modifying polyethyleneimine with different molecular weights with phenylboronic acid compounds. The modified polyethyleneimine has an active targeting property for tumor cell membranes, and can carry genes to form a gene vector composition used for gene therapy. The gene vector composition is uniform in particle size, proper in charge, stable in properties, low in cell toxicity, high in cell transfection efficiency, high in target power, and good in biocompatibility and biodegradability. The modified polyethyleneimine and the gene vector composition can be used for the gene therapy. Preparing processes of the modified polyethyleneimine and the gene vector composition are simple, high in reaction degree controllability and capable of industrial production.

The invention relates to a preparation method of a pesticide slow controlled release nanometer composite material, in particular to a preparation method of a pesticide diuron slow controlled release nanometer composite material. The preparation method is characterized by comprising the following steps of: (1) dissolving diuron in water and dispersing by using ultrasound to obtain suspension containing the diuron; (2) loading the solution containing the diuron on hydroxyapatite nanometer granules by adopting a negative pressure and freeze drying method to obtain drug loading hydroxyapatite nanometer granules; (3) mixing and stirring sodium alga acid solution and hydroxyapatite nanometer granule suspension to obtain a sol system; dripping the sol system into a calcium ion cross-linking agent solution by an injector to obtain ion crosslinking gel granules of alginic acid/drug loading hydroxyapatite nanometer granules; washing by using distilled water, and drying in vacuum to obtain the micro-granular pesticide diuron slow controlled release nanometer composite material. The system can control the pesticide to slowly release and improve the pesticide effect. The invention solves the problem of pollution caused by too frequent pesticide use, too great dosage, low utilization rate of medicament, poor effect and medicament loss.

The invention discloses a folic acid mediated antitumor drug superparamagnetic tumor targeted nanoparticle. A superparamagnetic iron oxide nanoparticle is adopted as the carrier, and high temperature thermal decomposition method is employed to synthesize polyethylene glycol-polyethyleneimine modified uperparamagnetic iron oxide nanoparticle, then a folic acid ligand is grafted on the surface of iron oxide by chemical method, and then an antitumor drug is loaded into the iron oxide nanoparticle by electrostatic adsorption and hydrogen bonding, thus obtaining the folic acid mediated antitumor drug superparamagnetic tumor targeted nanoparticle. According to the invention, the dual targeting effect can strengthen the anti-tumor effect of the antitumor drugs, and the folic acid mediated antitumor drug superparamagnetic tumor targeted nanoparticle has small toxic and side effect, thus being a tumor treatment nanoparticle preparation with substantial development value.

The invention relates to the field of biological medicines, and particularly provides an antitumor drug carrier and an application method thereof. The antitumor drug carrier is a phycobiliprotein nano particle. On the basis of the difference between normal cells and tumor cells, the phycobiliprotein nano particle without toxicity and immunogenicity is adopted as the antitumor drug carrier; the tumor-targeting capability is given; meanwhile, antitumor drugs are efficiently loaded; the antitumor drugs specifically reach the tumor lesion part; the treatment effects of high selectivity and low toxicity are realized; and the defects that a traditional cytotoxic drug is poor in selectivity and relatively high in toxic and side effect, and the drug resistance is easily generated are overcome. The preparation method provided by the invention is simple and feasible; and the prepared product can be stably stored in a water solution and is beneficial to storage.

The invention discloses a compound sulfamonomethoxine sodium multivesicular liposome and a preparation method thereof. The compound sulfamonomethoxine sodium multivesicular liposome is prepared from lipid, sulfamonomethoxine sodium as a hydrophilic medicament and trimethoprim as a lipophilic medicament, wherein the sulfamonomethoxine sodium and the trimethoprim are simultaneously packaged in the multivesicular liposome structure formed from soya bean lecithin, cholesterol and triacylglycerol; when the multivesicular liposome is synthesized, the addition ratio of the total mass of the sulfamonomethoxine sodium and the trimethoprim to the total mass of lipid is preferably 1:4; the added mass ratio of the sulfamonomethoxine sodium to the triacylglycerol is preferably 4:1; the added mass ratio of the soya bean lecithin to the cholesterol is prefertably 1:1; and the use level of the triacylglycerol is preferably 16/mmol*L-1. During preparation, the ratio of the first emulsification time to the second emulsification time is preferably 4:1 (12 min:3 min). In the liposome, two medicaments with different dissolving characteristics of water solubility and lipid solubility are mixed together to be applied to the same multivesicular liposome; and the multivesicular liposome has the advantages of long slow releasing time, high packaging rate, good stability, can be used as a new preparation of veterinary medicine and lays foundations for clinical generalization and application.

The present invention discloses a module compound with hydrogen bond sequence specificity combination and a preparation method thereof. The structure formula of the compound module is as follows: wherein, when R is (m is any integer of 1-200), the module compound is a hydrophilic module compound P2; when R is (n is any integer of 2-140), the module compound corresponds to the hydrophobic module compound of BLOCK-1, BLOCK-2 or BLOCK-3. As the module compound has hydrogen-sequence specificity and the two complementary single-stranded can mutually recognize and match with specificity, the block copolymer which is not easy to synthesize by other methods can be easily prepared by using a molecular glue group.

The present invention belongs to the technical fields of biomedical medicine and nano-medicine, and relates to a stem cell tumor targeting system with internal nano prodrug and a preparation method thereof. The method uses stem cells as cell carriers to prepare an anti-tumor targeting system with general formula of Stem cells-(RGD-PPCD)n through endocytosis and inner load of nano prodrug. The nano prodrug has the characteristics of targeting, sustained release and acid sensitivity release; and the stem cell carrier can remotely target primary lesion and metastasis of tumor. In vitro experimental results show that the stem cells after drug load can retain the proliferation capacity and tumor migration characteristics; and the constructed system is stable to ensure that the drug stays in the form of nano-prodrug for several days in the cells, and can slowly release the drug. In vivo experimental results show that the constructed stem cell targeting system can significantly prolong the survival time of tumor-bearing animals and can maintain normal neurobehavioral characteristics of tumor-bearing animals in comparison with the original drug and nano prodrugs, so as to play the efficient, secure and long-lasting anti-tumor effect.

The invention discloses a tumor-targeted photodynamic medicine carrying nanoparticle as well as a preparation method and an application thereof, belonging to a medical preparation containing porphyrin or a medical preparation prepared by a method which utilizes wave energy to process materials. According to the invention, porphyrin or derivates of porphyrin of a functional group and polyethylene glycol and polypropylene glycol of a polyether chain segment are connected through chemical bonds or ester bonds or amido bonds of perssads, and then polymerized into a nanoparticle aqueous dispersion of porphyrin or derivates, loaded with a chemotherapy drug with a conjugated structure and dialyzed to obtain the tumor-targeted photodynamic medicine carrying nanoparticle. The nano material prepared by the method is high in yield, regular in shape, uniform in distribution and good in biosecurity, can be effectively loaded with an antitumor drug with a conjugated structure; a water-soluble photosensitizer can effectively reverse drug tolerance of a chemotherapy drug after being irradiated by near-infrared light; the nanoparticle can efficiently target and position tumors, has good anelasticity, effectively inhibits tumor growth, and has a wide application prospect in preparing breast cancer tumor-targeted drugs.

The invention discloses a pH redox dual sensitive PAMAM (Polyamidoamine) targeted nano drug delivery carrier. A general formula is T-PEG[n]-SS-PAMAM-(His)[m], wherein T is a tumor targeting ligand, the PAMAM is a cationic polyamidoamine-dendritic macromolecule, PEG is polyethylene glycol, -SS- is a bridge bond disulfide bond, His is histidine, n is 20 to 1000, and m is 1 to 4096. The invention also provides a preparation method of the pH redox dual sensitive PAMAM targeted nano drug delivery carrier. By modification of the His, the acid sensitivity of the carrier can be improved, a proton sponge effect of the PAMAM is enhanced, and a drug is enabled to quickly escape from acidic lysosome.

A targeting nanoparticle of 10-hydroxy camptothecine for treating tumor is proportionally prepared from gelatin, 10-hydroxy camptothecine, tert-butanol, VC, chitosan, carboxymethyl cellulose sodium, and the water for injection. Its preparing process is also disclosed.

The invention discloses a nano reagent based on melittin and a preparation method and an application thereof. The nano reagent is prepared by melittin, an organic dye, and hyaluronic acid. The preparation of the nano reagent is simple, hemolytic activity of melittin is greatly reduced, and the nano reagent can realize in-vivo tumour fluorescence imaging and obvious antineoplastic curative effect.The nano reagent can be effectively enriched at a tumour area after being subjected to intravenous injection in body, and can track the distribution condition of the nano reagent in the body through afluorescence signal, besides the chemotherapeutic anticancer activity of melittin, the organic dye components in the nano reagent can generate response on laser irradiation and increases the anticancer effect, so that the chemotherapy and phototherapy-combined for anticancer can be realized. In addition, the bio-security of the agent is good, the nano reagent can be widely used for preparing in-vivo cancer diagnosis and treatment-integrated medicines, and in-vivo treatment for melittin is realized.

The invention provides a tumor cell membrane drug loading system as well as a construction method and application thereof. The drug loading system comprises a tumor cell membrane and a polypeptide drug connected to the surface of the tumor cell membrane. The tumor cell membrane has good biocompatibility, so that the half-life period of the polypeptide drug in vivo can be prolonged; the tumor-associated antigen capable of being expressed on the surface of a tumor cell membrane can play a role of a tumor vaccine after being phagocytized by macrophages; homogeneous adhesion antigens can be expressed on the surfaces of tumor cells, so that the drug loading system is actively targeted to tumor parts, and active targeted drug delivery is realized. Meanwhile, the contrast agent is wrapped in the tumor cell membrane, and the contrast agent is gathered at the tumor part and the concentration of the contrast agent is increased by utilizing the active targeting property of the tumor cell membrane to the tumor part, so that the nuclear magnetic imaging effect of the tumor part is enhanced. Therefore, the tumor cell membrane drug loading system has important significance for realizing tumor diagnosis and treatment integration.

The invention belongs to the field of molecular imaging probes and particularly relates to a preparation method of a dual-modal nano imaging drug Dex-Rho-99mTc based on glucan, and an application of the drug in imaging diagnosis. The general formula of the drug is Rho-Dex-PEG-DTPA-99mTc, wherein the Dex represents for the glucan of which the molecular weight is 10-100k, the PEG represents for polyethylene glycol of which the molecular weight is 1-10k, the Rho represents for a fluorescent group rhodamine, the DTPA is a chelating agent of an imaging nuclide and the 99mTc is a radioisotope Technetium-99 used for SPECT imaging. In the invention, the surface of the high-molecular material glucan is modified by a certain number of amino groups and the PEG, the fluorescent group and the SPECT imaging groups are connected to the glucan supporter through the amino groups and finally the drug is marked by the radioisotope [99mTc]. The drug is good in biocompatibility, is simple in the preparation method, is safe and convenient to use and can be employed in dual-modal imaging. The dual-modal nano imaging drug has wide application prospects in the biomedical fields of early diagnosis of cancer, medicine delivery under guide of imaging, noninvasive iconography curative effect evaluation and the like.

The invention provides a targeting responsive release system. The targeting responsive release system comprises a chimeric apoptotic corpuscle, the chimeric apoptotic corpuscle comprises an apoptoticcorpuscle membrane and mesoporous nanoparticles wrapped in the apoptotic corpuscle membrane, the apoptotic corpuscle membrane is an apoptotic corpuscle membrane derived from immune cells, and the mesoporous nanoparticles are loaded with active substances. The system provides an effective means for constructing a specific treatment product with a targeted delivery function and an accurate drug release function.

The invention discloses an active targeted gene delivery nanoparticle, and a preparation method and application thereof. The active targeted gene delivery nanoparticle consists of a cationic polymer,hyaluronic acid and a negatively charged gene. The cationic polymer and the negatively charged gene form a composite core; the hyaluronic acid coats on the surface of the composite core by charge action and chemical bond bonding; and the cationic polymer is one of PAsp (EDA), PAsp (DET), PAsp (TET) and PAsp (TEP), or one of chemically modified derivatives of the PAsp (EDA), PAsp (DET), PAsp (TET)and PAsp (TEP). The hyaluronic acid used in the invention has excellent biocompatibility, not only reduces the cytotoxicity caused by the cationic polymer, but also actively targets a hyaluronic acid-specific receptor which is highly expressed on surfaces of tumor cells, theeby more efficiently delivering exogenous source genes into the tumor cells, and increasing cellular uptake and transfectionefficiency.

The invention discloses a T1 strengthened nanometer magnetic contrast agent having the function of activating targeting properties of blood platelets and a preparation method of the T1 strengthened nanometer magnetic contrast agent belonging to the field of nuclear magnetic resonance contrast agents. The characteristics of "dissociation-self-assembly" and surface modificability of an apoferritin nanometer cage under different pH environment are utilized, paramagnetic Gd-DOTA is loaded into the apoferritin nanometer cage, ligand-KGDS tetrapeptide specifically targeting and activating the bloodplatelets is coupled to the surface of Gd-loaded apoferritin, and therefore, nanometer particles which can actively target and activate the blood platelets and have nuclear magnetic resonance T1 weighted imaging strengthened contrast effects can be obtained. The particle diameter of the nanometer contrast agent is about 10nm, the nanometer contrast agent has favorable contrast effects, targeting properties for activating the blood platelets, and biocompatibility; precise visualization of thrombosis is hopefully realized through targeting and activating the blood platelets, and more comprehensive molecule information is provided for early prevention, diagnosis and treatment of cardiovascular diseases.

The invention discloses an amino acid sequence specifically combined with mycobacterium tuberculosis or a derivative of the amino acid sequence. The amino acid sequence or the derivative of the aminoacid sequence contains an amino acid sequence shown as SEQ ID NO: 1. The invention also discloses a nucleotide sequence for encoding the amino acid and application of the amino acid sequence. The amino acid sequence disclosed by the invention can be specifically combined with mycobacteria and is used for diagnosing tuberculosis, the diagnosis sensitivity and specificity are improved, and a new method is provided for targeted diagnosis of tuberculosis.