Tumor cell membrane drug loading system as well as construction method and application thereof
A construction method and cell membrane technology are applied to the tumor cell membrane drug-carrying system of long-circulating polypeptide drugs in vivo and the field of construction thereof, which can solve the problems of inability to produce effective blood drug concentration, low oral bioavailability, and reduced patient compliance, etc. Enhanced MRI effect, good biocompatibility, and increased concentration
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0106] This embodiment provides a tumor cell membrane drug-carrying system, the preparation method of which includes the following steps:
[0107] 1. Preparation of tumor cell membrane
[0108] H460 cells were cultured in RPMI-1640 complete medium containing 10% fetal bovine serum and 1% double antibodies (penicillin and streptomycin) at 37°C in 5% CO 2 cultured in a cell culture incubator until the cell density reached 80%.
[0109] Wash twice with PBS, digest with trypsin diluted 4 times in PBS containing 2mM EDTA, centrifuge at 300g, wash twice with PBS, collect cells and resuspend in hypotonic buffer containing protease inhibitors, rotate at 80rpm at 4°C 1h, sonicate in an ice bath for 30min, sonicate with an ultrasonic cell disruptor for 3min to disrupt the cells, centrifuge at 20,000g for 20min, discard the precipitate, and centrifuge the supernatant at 100,000g for 1h at 4°C to harvest the cell membrane pellet. Then use PBS solution to resuspend, and sonicate for 5 mi...
Embodiment 2
[0135] This example is used to study the nuclear magnetic properties of the cell membrane drug-loaded system.
[0136] The cell membrane prepared in embodiment 1 is wrapped with Fe 3 o 4 The drug-loading system (mem / Fe 3 o 4 ) by Fe 3 o 4 The concentration is made into a series of aqueous solutions with different concentrations, Fe 3 o 4 Concentrations were 20 μg / mL, 10 μg / mL, 5 μg / mL, 1 μg / mL and 0.2 μg / mL, and the Fe 3 o 4 T2 imaging and relaxation time T2 before and after wrapping with cell membrane, different concentrations of Fe 3 o 4 and mem / Fe 3 o 4 The magnetic resonance analysis images of Fe are shown in Figure 6(a) and Figure 6(b). 3 o 4 , mem / Fe 3 o 4 Still has a good imaging effect;
[0137] The relaxation rate r2 of the sample is shown in Figure 6(c), the Fe wrapped in the cell membrane 3 o 4 There is some decrease in the relaxation rate, which may be due to the coating of the cell membrane covering the Fe to a certain extent 3 o 4 imaging effe...
Embodiment 3
[0139] This example detects and studies the cytotoxicity of the cell membrane drug-loaded system.
[0140] Naked Fe 3 o 4 and membrane-wrapped Fe 3 o 4 Drug loading system, according to Fe 3 o 4 The amount of CCK8 (CellCounting Kit-8) reagent was added to each well after being co-cultured with H460 cells in a 37°C cell culture incubator for 24 hours. The standard instrument detects the absorbance at 450nm.
[0141] The result is as Figure 7 As shown, the exposed Fe 3 o 4 No cytotoxicity, and the Fe after cell membrane wrapping 3 o 4 It has stronger biocompatibility and can better promote cell proliferation.
PUM
Property | Measurement | Unit |
---|---|---|
Particle size | aaaaa | aaaaa |
Particle size | aaaaa | aaaaa |
Thickness | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com