Preparation method of HA/RGD double-receptor multi-target-point drug administration system

Abstract

The invention discloses a preparation method of a HA / RGD double-receptor multi-target-point drug administration system.The method includes the following steps that hyaluronic acid and hydrazine sulfate are dissolved in hydrazine, reaction is performed for 1-12 h under the protection of inert gas at the temperature of 60-115 DEG C so that deacetylated HA can be obtained; RGD is dissolved in water, an EDC / NHS solution is activated, pH of the solution is adjusted to be 3.5-6.5, the deacetylated HA aqueous solution is dropwise added, reaction is performed for 1-12 h at 4-38 DEG C, and HA-RGD is obtained; a CLB-ADH aqueous solution is dropwise added into the HA-RGD aqueous solution, reaction is performed for 1-12 h under the condition of stirring, and the double-receptor multi-target-point drug administration system is obtained.HA and RGD can more effectively enhance combination of drugs and tumor cells, effective concentration on the tumor parts is improved, an effect-enhancing and toxicity reducing effect is achieved, meanwhile tumor cells are killed, growth of tumor microvasculature is inhibited, the effect that one drug is multipurpose is achieved, and tumor drug resistance can be effectively reversed.

Description

Technical field

[0001] The invention relates to the field of medicines, in particular to a preparation method of a dual receptor-mediated multi-target drug delivery system. Background technique

[0002] Cancer is one of the serious diseases endangering human life and health, and the incidence is increasing year by year. It is urgent to find effective prevention and treatment methods for malignant tumors. Chemotherapy is one of the main methods for cancer treatment today, but chemotherapeutic drugs often involve normal cells when they act on target cells, resulting in toxic side effects such as weakened immune function, bone marrow suppression, and organ damage, severe side effects and multi-drug resistance. Drugs and other issues have greatly restricted its application.

[0003] Targeted drug delivery system is an ideal drug delivery method for a new type of drug formulation. Using the characteristics of the targeted drug delivery system, the release of drugs to specific tissues,...