4results about How to "Low dark toxicity" patented technology

The application discloses a MOF-based high-order multi-photon excitation fluorescent material and a preparation method and application thereof, relates to the technical field of multi-photon excitation fluorescent materials, and the preparation method comprises the following steps: 1) preparation of ZrTc; 2) preparation of Au NRs; 3) preparation of Au NR / ZrTc; and 4) preparation of Au NR / ZrTc@FA. The MOF-based high-order multi-photon excitation fluorescent material prepared by the application has good biocompatibility and low dark toxicity, can realize enhanced two / three-photon fluorescence imaging under near-infrared two-region laser irradiation, and can be applied to cancer cell specific targeting and deep tissue fluorescence imaging.

The invention discloses a novel ring-substituted and side-chain amino-substituted nile blue dye and a synthesis method and application thereof, a series of dyes which can be simultaneously used for bacterial fluorescence imaging and photodynamic antibacterial treatment are designed and synthesized by taking the nile blue dye as a parent, and the structure of the novel ring-substituted and side-chain amino-substituted nile blue dye is as shown in a general formula I, the dye has high molar extinction coefficient, fluorescence imaging efficiency and active oxygen generation efficiency, and MNBS-C2-NH2 and MNBS-C6-NH2 synthesized in the embodiment show excellent absorption and fluorescence properties in PBS (Phosphate Buffer Solution) and dichloromethane, especially in a range of 640-660nm. A test result shows that the dyes have a remarkable killing effect on escherichia coli under an illumination condition, log CFU is obviously reduced, and dark toxicity is relatively low. Scanning electron microscope observation further proves that the bacteria subjected to light treatment have obvious membrane surface collapse and rupture phenomena. Through the outer membrane protein of the gram-negative bacteria, the enhanced penetrating ability and killing ability on the gram-negative bacteria can be realized, and wide application prospects are realized in the field of antibacterial treatment.

The application discloses a kind of tannin-based Mn-Fe diatomic metal polyphenol nano-carrier loaded hypocrellin B nano-assemblies and its preparation method and application.The nano-assemblies use tannin as ligand, by step coordination driven self-assembly strategy, first with Mn 2+ Forming pre-assembly template, then introducing Fe 3+ Constructing Mn-Fe diatomic metal polyphenol network with atomic level uniform dispersion, and loading traditional Chinese medicine source photosensitizer hypocrellin B to obtain nano-preparation.The preparation realizes Fe 2+ / Fe 3+ Self-circulation, and can sustainably catalyze H2O2 in tumor microenvironment to generate hydroxyl radical, realizes long-acting chemical kinetics treatment;Meanwhile, in-situ oxygen production relieves tumor hypoxia, improves photodynamic therapy effect, and consumes glutathione to amplify oxidative stress.The preparation process of the application is mild, and biocompatibility is good, and it breaks through the bottleneck of insufficient effect of traditional treatment in anoxic tumor.

The invention relates to preparation and application of tetracoumarin-based beta-beta coupled double-BODIPY near-infrared photothermal dye, which are realized by the following method: carrying out C-C coupling reaction on a BODIPY monomer derivative (I) and anhydrous ferric trichloride to obtain a beta-beta coupled double-BODIPY derivative (II), and carrying out reaction on the beta-beta coupled double-BODIPY derivative (II) and 7-(N, N-dimethyl formamide)-1, 2, 3, 4-tetracoumarin-based beta-beta coupled double-BODIPY derivative (II) to obtain the tetracoumarin-based beta-beta coupled double-BODIPY near-infrared photothermal dye. The preparation method comprises the following steps: carrying out Knoevenagel condensation reaction on N, N '-diethylamino) coumarin-3-formaldehyde to obtain the near-infrared photothermal agent tetracoumarin-based beta-beta coupled double BODIPY derivative (III). The preparation method has the advantages of simple reaction steps, mild reaction conditions, good selectivity and wide application range. The photothermal agent has relatively strong near-infrared absorption, excellent photothermal conversion efficiency and cell apoptosis ability, can be used as a novel photothermal therapy anti-tumor and antibacterial drug, and has important scientific significance and application value.