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Nano reagent based on melittin and preparation method and application thereof

A melittin and nanotechnology, applied in the field of melittin-based cancer diagnosis and treatment nano-reagents and their preparation, can solve the problems of reduced anti-cancer efficacy, hemolysis, melittin leakage, etc., and achieve the improvement of blood compatibility, inhibition of The effect of tumor growth and strong tumor targeting

Active Publication Date: 2018-06-29
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current melittin drug-loading system still has the following defects: first, many nanoparticles loaded with melittin are not stable enough, and there may be problems of melittin leakage and potential hemolysis; secondly, most melittin The delivery system has no fluorescence, so the distribution and enrichment of melittin in the organism cannot be traced; more importantly, these melittins loaded in nanocarriers have a much greater anticancer effect than free melittins. lower, the dose needed to kill cancer cells is multiplied

Method used

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  • Nano reagent based on melittin and preparation method and application thereof
  • Nano reagent based on melittin and preparation method and application thereof
  • Nano reagent based on melittin and preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0032] The synthesis process of nano-reagents for cancer diagnosis and treatment based on melittin is as follows:

[0033] Step 1: Mix 1 mL of melittin aqueous solution (1 mg / mL) with 1 mL of Ce6 aqueous solution (2 mg / mL), sonicate in a water bath for 5 minutes, and stir overnight at room temperature; Carry out dialysis purification in;

[0034] Step 2: Add the complex aqueous solution obtained above dropwise to 6 mL of HA aqueous solution (0.5 mg / mL), the weight-average molecular weight of HA is 700,000, and stir overnight at room temperature to obtain a nano-reagent based on melittin .

[0035] The reagent obtained by the above method is recorded as melittin / Ce6@HA, and the schematic diagram of its preparation process is shown in figure 1 .

Embodiment 2

[0037] The synthesis process of nano-reagents for cancer diagnosis and treatment based on melittin is as follows:

[0038] Step 1: Mix 1 mL of melittin aqueous solution (1 mg / mL) with 1 mL of carbocyanine dye Cypate aqueous solution (3 mg / mL), sonicate in a water bath for 5 minutes, and stir overnight at room temperature; Purified by dialysis in pure water;

[0039] Step 2: Add the complex aqueous solution obtained above dropwise into 6 mL of HA aqueous solution (0.5 mg / mL), the average molecular weight of HA is 3500, and stir overnight at room temperature to obtain a nano-reagent based on melittin.

Embodiment 3

[0041] The synthesis process of nano-reagents for cancer diagnosis and treatment based on melittin is as follows:

[0042] Step 1: Mix 1 mL of melittin aqueous solution (1 mg / mL) with 1 mL of protoporphyrin aqueous solution (10 mg / mL), ultrasonicate in a water bath for 5 minutes, and stir overnight at room temperature; Purified by dialysis in pure water;

[0043] Step 2: Add the complex aqueous solution obtained above dropwise into 6 mL of HA aqueous solution (1 mg / mL), the average molecular weight of HA is 1,500,000, and stir overnight at room temperature to obtain a melittin-based nano-reagent.

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Abstract

The invention discloses a nano reagent based on melittin and a preparation method and an application thereof. The nano reagent is prepared by melittin, an organic dye, and hyaluronic acid. The preparation of the nano reagent is simple, hemolytic activity of melittin is greatly reduced, and the nano reagent can realize in-vivo tumour fluorescence imaging and obvious antineoplastic curative effect.The nano reagent can be effectively enriched at a tumour area after being subjected to intravenous injection in body, and can track the distribution condition of the nano reagent in the body through afluorescence signal, besides the chemotherapeutic anticancer activity of melittin, the organic dye components in the nano reagent can generate response on laser irradiation and increases the anticancer effect, so that the chemotherapy and phototherapy-combined for anticancer can be realized. In addition, the bio-security of the agent is good, the nano reagent can be widely used for preparing in-vivo cancer diagnosis and treatment-integrated medicines, and in-vivo treatment for melittin is realized.

Description

technical field [0001] The invention belongs to the field of biological materials, and relates to a melittin-based nanometer reagent, in particular to a melittin-based cancer diagnosis and treatment nanometer reagent and its preparation method and application. Background technique [0002] Melittin is a cytolytic polypeptide derived from bee venom. A large number of studies have shown that melittin has extremely strong pharmacological effects and biological activities, and has potential clinical application value in many fields such as anti-inflammation, labor pain, anti-microbial, anti-virus and anti-tumor. Especially in cancer treatment, compared with traditional chemotherapy drugs, melittin is less likely to make cancer cells develop drug resistance. However, melittin can cause severe hemolytic reaction after intravenous injection and damage normal tissues, so the application of melittin in vivo has been greatly limited. In recent years, with the continuous development ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61K41/00A61K47/36A61K49/00A61P35/00
CPCA61K38/1767A61K41/0071A61K41/0076A61K47/36A61K49/0034A61K49/0036A61K49/0054A61K2300/00
Inventor 吴富根贾浩然祝雅璇
Owner SOUTHEAST UNIV
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