60results about How to "Enables fluorescence imaging" patented technology

The invention provides a composite nano-grade novel material based on cancer early-stage integrated detection, diagnoses, and treatment, and a preparation method thereof. According to the invention, gold nano-cage (gold nano-box) and nano-grade iron oxide are assembled, such that gold nano-cage (box)/nano-grade iron oxide composite particles are synthesized. With an assembling method from the inside to the outside, the gold nano-cage (box) is first prepared; iron oxide is nucleated and grown in the gold nano-cage (box); with a surfactant (such as glucan or the like), mono-dispersed iron oxide is obtained; the iron oxide is packaged by using biological molecules, such that the gold nano-cage (box)/nano-grade iron oxide composite particles are obtained; surface modification is carried out by using biological targeting molecules (antibodies or DNA molecules). The material is used in tumor magnetic resonance imaging, fluorescence imaging, dark field imaging, specific targeting multifunctional diagnosis, and photothermal therapy. The material can also be used as a targeting medicine carrier.

The invention discloses a quaternary ammonium salinization fluorescence silicon dot which comprises a silicon-containing nanoparticle and alkyl betaine subjected to covalent grafting on the surface of the nanoparticle. The invention also discloses a preparation method of the quaternary ammonium salinization fluorescence silicon dot and application thereof in aspects such as bacteria resistance and bacteria imaging. Compared with the prior art, the invention has the advantages that a silicon dot of which the surface is provided with amino is subjected to quaternary ammonium salinization through a one-step reaction by utilizing the alkyl betaine, high water solubility of the silicon dot is maintained, and the silicon dot is endowed with excellent selective bacteria resistance and bacteria imaging effects. Moreover, the quaternary ammonium salinization silicon dot has the advantages of high purity degree, high stability, low preparation cost and the like, and a fluorescent marker does not need to be added.

The invention relates to a selenocysteine near-infrared fluorescent probe, and a preparation method and application thereof, belonging to the field of chemical analysis and detection. The near-infrared fluorescent probe Fsec-3 for detecting selenocysteine provided by the invention has the advantages of good selectivity, high sensitivity, low detection limit (20 nM) and good biocompatibility. In aphosphate buffer, the fluorescence intensity and the concentration of selenocysteine (0-100[mu]M) are in a good linear relationship, which indicates that the probe Fsec-3 is suitable for quantitativedetection of selenocysteine. The probe Fsec-3 achieves fluorescence imaging of selenocysteine in the human breast cancer cell MCF-7; and the probe Fsec-3 also achieves sensitive detection of endogenous selenocysteine in a living body, and is fast in response and stable in fluorescence signals. Thus, the fluorescent probe Fsec-3 prepared by the invention is an effective tool for visual and quantitative detection of the content of selenocysteine in cells, living bodies and tumor tissue.

The invention relates to a carbon dot used as an antitumor drug and a preparing method and application of the carbon dot. With arginine being a carbon source, arginine is completely dissolved with water, and an appropriate amount of thylenediamine is added and subjected to ultrasonic dissolving and microwave heating to obtain the target product carbon dot. Since cancer cells themselves in an organism contain hydrogen peroxide, the carbon dot can be used for treating cancer. The carbon dot can not only conduct fluorescence imaging, but also has the advantages of being high in biocompatibility,large in NO releasing amount, long in acting circular time, high in drug stability, cheap, easy to obtain and the like.

The invention discloses a nano reagent based on melittin and a preparation method and an application thereof. The nano reagent is prepared by melittin, an organic dye, and hyaluronic acid. The preparation of the nano reagent is simple, hemolytic activity of melittin is greatly reduced, and the nano reagent can realize in-vivo tumour fluorescence imaging and obvious antineoplastic curative effect.The nano reagent can be effectively enriched at a tumour area after being subjected to intravenous injection in body, and can track the distribution condition of the nano reagent in the body through afluorescence signal, besides the chemotherapeutic anticancer activity of melittin, the organic dye components in the nano reagent can generate response on laser irradiation and increases the anticancer effect, so that the chemotherapy and phototherapy-combined for anticancer can be realized. In addition, the bio-security of the agent is good, the nano reagent can be widely used for preparing in-vivo cancer diagnosis and treatment-integrated medicines, and in-vivo treatment for melittin is realized.

The invention relates to a phosphorus-doped fluorescent carbon point with a high fluorescent quantum yield as well as a preparation method and application thereof. According to the technical scheme, purified carbon point solid powder is obtained by taking tannic acid and polyethylenimine (PEI), adding de-ionized water and phosphoric acid, then transferring the mixture into a hydrothermal reaction kettle, keeping the heat and reacting for 2h to 6h in a temperature range of 140 DEG C to 240 DEG C, naturally cooling at a room temprature to obtain a light brown nano carbon point crude product solution, and centrifuging, dialyzing, refining, freezing and drying. The carbon point provided by the invention can be used for cell imaging and a photodegradation treatment process of contained organic dyestuffs. The fluorescent quantum yield of the prepared fluorescent carbon point can reach 25.4 percent. After being stored in a dark place for 6 months, the carbon point still can emit bright blue fluorescent light under the illumination of a 365nm ultraviolet lamp and the carbon point has good stability at a room temperature.

The invention discloses a virus-like hollow oxide loaded near-infrared two-b region excited rare earth nanocrystal and a preparation method and application thereof. According to the nanocrystal and the preparation method, an Er and Ho doped NaErF4:2%Ho@NaYF4 rare earth nanomaterial is synthesized through a hydrothermal method, then the nanomaterial is modified on a surface of a virus-like hollow manganese oxide poured into IR1064 through an amidation reaction, and a fluorescent composite probe which is good in biocompatibility and responds to a tumor microenvironment is obtained. According to the fluorescent composite probe, after the manganese oxide is degraded at a tumor, manganese ions are used for chemodynamic therapy and nuclear magnetic resonance imaging contrast agents of metastasis, released rare earth nanocrystals have excitation of 1530 nm and emitted lights of 650 nm and 1180 nm, the light of 1180 nm can be used for tumor imaging navigation surgical resection with no background interference and high resolution and tissue penetrating power in a near-infrared second region, and the light of 650 nm can used for near-infrared first region fluorescence imaging.

The invention discloses a preparation method of a poly(N-isopropyl acrylamide) sphere modified fluorescent silicon dot, the prepared fluorescent silicon dot and application. The preparation method comprises the following steps: reacting sodium citrate and 3-aminopropyl triethoxysilane in glycerol to prepare a silicon nanocrystalline quantum dot; reacting silicon nanocrystalline quantum dots, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and folic acid in pure water to obtain folic acid modified silicon nanocrystalline quantum dots; and reacting N-isopropylacrylamide, N,N'-methylene diacrylamide, the folic acid modified silicon nanocrystalline quantum dots and potassium persulfate in pure water to obtain the fluorescent silicon dots modified by the polyisoacrylamide spheres. The polyisoacrylamide sphere modified fluorescent silicon dot prepared by the method is low in cytotoxicity, has a function of responding to temperature change, can be used for cancer cell in-situ imaging and temperature detection, and is simple and feasible in preparation process and easy for large-scale production.

The invention relates to a near-infrared fluorescent probe for hydrogen polysulfides, and a preparation method and application of the near-infrared fluorescent probe, and belongs to the field of chemistry and analysis detection. The near-infrared fluorescent probe NIR-CPS for detecting hydrogen polysulfides has relatively large stokes shift (100nm), and has the advantages of good selectivity, highsensitivity, low limit of detection (18nM) and good biocompatibility. In a phosphate buffer saline (PBS), fluorescence intensity shows a good linear relation with the concentration of hydrogen polysulfides, so that the probe is suitable for quantitatively detecting hydrogen polysulfides. The probe NIR-CPS also realizes fluorescence imaging of hydrogen polysulfides in MCF-7 cells. More importantly, the probe NIR-CPS also realizes sensitive detection of endogenous hydrogen polysulfides generated by induction of lipopolysaccharide at a living body level, and is relatively high in response and relatively stable in fluorescence intensity.

The invention discloses a near-infrared molecular probe based on malonaldehyde response, a preparation method and application of the near-infrared molecular probe based on malonaldehyde response, and the near-infrared molecular probe based on malonaldehyde response takes xanthene-derived organic dye as a parent structure and contains an electron withdrawing group nitryl and a malonaldehyde reaction site benzoyl hydrazine group. The molecular conjugation degree is changed through the nucleophilic reaction of the hydrazine group of the o-nitrobenzoyl hydrazine and the MDA, so that the change of absorbance is caused, and the MDA detection is realized. The invention provides application of the molecular probe, a nano-probe NPs for MDA detection and imaging is prepared, the nano-probe takes near-infrared absorption CS-R and amphiphilic polymer DSPE-PEG2000 as main bodies, and a conjugated system of the CS-R in the nano-probe is changed through an addition reaction of MDA, so that fluorescence imaging is realized, detection and imaging with high sensitivity and good specificity on MDA are realized, and the problems of short wavelength and poor penetration depth of a conventional malondialdehyde probe are greatly improved.

The invention discloses a three-dimensional blood flow velocity imaging method and device based on lamellar light. The three-dimensional blood flow velocity imaging method comprises the following steps of performing layered irradiation on a sample through the lamellar light; collecting each layer of signals scattered when the layered light irradiates the sample, and for each layer, collecting a frame of image at a time interval of 1/f to obtain m frames of original blood flow images until the images at all the layers are collected; constructing the m frames of original blood flow images to obtain dynamic blood flow images at the th layer until dynamic blood flow images of all layers are obtained; performing three-dimensional reconstruction on the dynamic blood flow images of all layers toobtain three-dimensional blood vessel distribution; and calculating the three-dimensional blood vessel speed according to the three-dimensional blood vessel distribution. The device comprises a lamellar light irradiation system, a collecting system and a processing system. Compared with the prior art, the three-dimensional blood vessel speed can be well obtained. The method and device are mainly applied to the technical field of image processing.

The invention belongs to the technical field of fluorescence labeling, and particularly relates to an amino-substituted chromene quinoline type fluorescence marker as well as preparation and application thereof. According to the invention, amino groups of aromatic amine compounds and two ortho-chlorine-containing aldehyde coumarins are subjected to a one-step cyclization reaction to obtain a series of novel nitrogen-containing heterocyclic compounds. The compound has excellent fluorescence emission performance and also has good light stability in different organic solvents. According to the invention, the problem of poor light stability of cyanine dyes is effectively solved, and different organelles can be marked at the cellular level. The nitrogen heterocyclic ring is introduced, so that the biocompatibility of the compound is improved, and a good foundation is laid for biological application. Besides, the raw materials of the reaction are cheap and easy to obtain, the steps for synthesizing the marker are simple and easy to operate, the production cost can be effectively reduced while the performance of the marker is not reduced, and certain commercial value is achieved.

The invention discloses a method for detecting trace Fe<3+>, Hg<2+> and Cu<2+> in living cells through double-channel fluorescence imaging. A triamine-ethylamine derivative called as a probe for shortserves as a fluorescence imaging probe for detection of trace Fe<3+>, Hg<2+> and Cu<2+> in living cells through double-channel fluorescence imaging, and the trace Fe<3+>, Hg<2+> and Cu<2+> in livingcells are detected through double-channel fluorescence imaging. By means of the method, detection of multiple target ions can be realized. The probe and Fe<3+>, Hg<2+> and Cu<2+> form a probe-Fe<3+> complex, a probe-Hg<2+> complex and a probe-Cu<2+> complex respectively, strong fluorescence with different wavelengths is emitted, and the method facilitates detection, is good in visibility, high insensitivity and good in selectivity and can realize real-time online detection of Fe<3+>, Hg<2+> and Cu<2+> ions in the cells.

The invention relates to a fusarium moniliforme SiC quantum dot fluorescence labeling method and particularly relates to a fluorescence labeling method for fusarium moniliforme SiC in different growth stages by cytotoxicity-free aqueous phase silicon carbide quantum dots, and a long-time-period fluorescence imaging technology for living cells. The method comprises the following steps of preparing a mixed solution of slant strain fusarium moniliforme and a PD culture medium; preparing a mixed culture medium of a mycete liquid-SiC quantum dot aqueous phase solution from the mixed solution and an SiC quantum dot aqueous phase solution at a volume ratio of 3:1; performing constant-temperature shaking culture on the mixed culture medium at a temperature of 28 DEG C and a speed of 200r/min for 3-10 days, thereby obtaining a fusarium moniliforme SiC quantum dot fluorescent mark; applying the mark to seedling root dip dyeing, thereby realizing dynamic monitoring, tracing and fluorescence imaging of a process that the fusarium moniliforme infects plant roots. The method is simple and effective, is low in cost and is capable of realizing fluorescence labeling of the fusarium moniliforme in different periods.

The invention relates to the technical field of chemical synthesis, in particular to a compound, a preparation method thereof, a fluorescent probe and an antitumor drug. The invention discloses a compound as shown in a formula (I) which is described in the specification. The compound is accurately targeted and recognized through a hyaluronic acid ligand-receptor mediation effect; the overexpressedesterase in tumor cells releases TP-PPh3 by destroying an ester bond between the TP-PPh3 and hyaluronic acid, and fluorescence quenched by aggregation of the TP-PPh3 can be recovered. At mitochondrial respiration period, the proton pump in the membrane transports protons to the space in the mitochondrial membrane to form a mitochondrial transmembrane potential with high negativity, so that triphenylphosphine cations have the mitochondrial targeting capability, a mitochondrial region of a tumor can emit red fluorescence under the irradiation of near-infrared light, and fluorescence imaging ofthe tumor part is realized. When a laser of 650-980nm is used for irradiating tumor tissues, the TP-PPh3 can convert absorbed light energy into heat energy, so that tumors are ablated, and photothermal therapy is realized.

The invention discloses a preparation method and application of electropositive polymer fluorescent carbon quantum dots with a gene vector function, and relates to the preparation method and application of the fluorescent carbon quantum dots. The invention aims at solving the problems of heavy metal ions, low yield of quanta and unstable fluorescent property in the existing semiconductor fluorescent nano particles. The preparation method comprises the following steps: 1, adding organic acid and a cationic high molecular polymer, performing ultrasonic dissolution at room temperature to form a mixed solution; 2, charging the mixed solution into a reaction kettle, and heating for reaction until the color of the mixed solution turns into black brown; 3, adding water with the volume being 2 times of that of the black brown solution into the black brown solution, removing solid by centrifuging, and reserving the solution; and 4, adding deionized water to the solution, uniformly mixing and then recentrifuging the solution, removing solid, and then performing dialyses treatment to obtain the water solution of polymer carbon quantum dots. The polymer fluorescent carbon quantum dots synthesized by the method have superior fluorescent stability and high yield of quanta which is over 60%. The preparation method is used for preparing the polymer fluorescent carbon quantum dots.

The invention discloses a near-infrared selenocysteine fluorescent probe as well as a preparation method and application thereof. The structure of the near-infrared fluorescent probe for detecting selenocysteine is shown as (I), and the near-infrared fluorescent probe has the advantages of high reaction speed, good selectivity, high sensitivity and low detection limit. Under the physiological pH value, after the fluorescent probe reacts with selenocysteine with different concentrations, the fluorescence intensity and concentration show a good linear relationship, and the fluorescent probe is suitable for quantitative detection of selenocysteine, is used for fluorescence imaging of selenocysteine in human lung cancer cells A549, and can quantitatively detect selenocysteine in cells.