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A kind of polyhydrogen sulfide fluorescent probe and its preparation method and application

A fluorescent probe, hydrogen sulfide technology, applied in chemical instruments and methods, fluorescence/phosphorescence, organic chemistry, etc., can solve the problems of easy aggregation, cannot be used in large quantities, easy to be photobleached, etc., and achieves good selectivity and detection limit. Low, high sensitivity effect

Active Publication Date: 2022-04-29
XUZHOU MEDICAL UNIV
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Problems solved by technology

[0005] Many fluorescent probes have been developed for the detection of polyhydrogen sulfide, but they face some problems in practical applications, such as easy aggregation of dyes at high concentrations to quench fluorescence, easy to be photobleached, and poor biocompatibility. Can not be used in large quantities, etc., still need to further develop fluorescent probes with good selectivity, high sensitivity, and suitable for biological imaging

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  • A kind of polyhydrogen sulfide fluorescent probe and its preparation method and application
  • A kind of polyhydrogen sulfide fluorescent probe and its preparation method and application
  • A kind of polyhydrogen sulfide fluorescent probe and its preparation method and application

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Embodiment approach

[0061] 1. Materials and instruments

[0062] 1.1 Reagents

[0063]

[0064]

[0065] The rest of the reagents were domestic analytically pure

[0066] 1.2 Instruments

[0067]

[0068] 1.3 Configuration of the solution

[0069]Preparation of probe solution: AIE-PS2 (6.47 mg, 0.01 mmol) was dissolved in dimethyl sulfoxide (10 mL) to prepare a probe solution with a concentration of 1 mM.

[0070] Na 2 S 4 Preparation of stock solution: Nitrogen gas was passed through 10 mL of deionized water for 15 min. Under nitrogen, the Na 2 S 4 (17.42mg, 0.1mmol) was dissolved in the above-mentioned deionized water to make a concentration of 10mM Na 2 S 4 Stock solution, according to the needs of the experiment, Na 2 S 4 The stock solution was diluted to a solution of 1.0mM-100μM for use.

[0071] Na 2 S 2 Preparation of stock solution (as H 2 S 2 The source according to the literature preparation method: Inorganic Chemistry, 2003, 42, 12, 3712-3714): Nitrogen gas wa...

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Abstract

The present invention relates to a polyhydrogen sulfide fluorescent probe and its preparation method and application, its fluorescent probe AIE-PS2 has a larger Stokes shift (254nm), good selectivity, high sensitivity, low detection limit (9nM ), high fluorescence response times (75 times) and good biocompatibility. In PBS buffer, the fluorescence intensity and Na 2 S 4 (0‑20μM) concentration has a good linear relationship, indicating that the probe is suitable for the quantitative detection of polyhydrogen sulfide in vivo; the probe AIE‑PS2 can detect endogenous and exogenous polyhydrogen sulfide at the cellular level Imaging, and further applied AIE‑PS2 to realize the fluorescence imaging of polyhydrogen sulfide in MCF‑7 cells, which is an effective tool for visual and quantitative detection of polyhydrogen sulfide. The structural formula of the fluorescent probe AIE‑PS2 is as follows.

Description

technical field [0001] The invention belongs to the field of chemistry and analysis and detection, and specifically relates to a polyhydrogen sulfide fluorescent probe based on the dual mechanisms of aggregation-induced luminescence and intramolecular proton transfer, and a preparation method and application thereof. Background technique [0002] Hydrogen sulfide (H 2 S) is an important gas signal molecule, the physiological concentration of H 2 S has a protective effect on cells and participates in processes such as nerve regulation, regulation of vascular tone, oxidative stress, inflammatory response, mitochondrial energy supply, and fibrosis. Polyhydrogen sulfide (hydrogen polysulfides, H 2 S n , n≥2) is H 2 The oxidation product of S belongs to sulfurane sulfur compound (sulfane sulfur). In living systems H 2 S and H 2 S n Coexist and jointly regulate the redox homeostasis in cells, and there is a cross-talk between the two in terms of biological activity and sig...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D277/66C09K11/06G01N21/64
CPCC07D277/66C09K11/06G01N21/64C09K2211/1007C09K2211/1037
Inventor 薛运生吴春丽张玲张君瑜刘云萍郑友广
Owner XUZHOU MEDICAL UNIV
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