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NIR-II fluorescence enhancement liposome and preparation method and application thereof

A technology of fluorescence enhancement and liposome, which is applied in the field of nanotechnology and fluorescence diagnosis research, can solve the problems of reducing fluorescence intensity and so on

Active Publication Date: 2019-12-20
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The problem is that when loaded into a hydrophilic polymer matrix or modified with ionic groups to increase the water solubility of hydrophobic molecules, it brings inevitable molecular aggregation and severe fluorescence quenching (ACQ), reducing the fluorescence intensity

Method used

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  • NIR-II fluorescence enhancement liposome and preparation method and application thereof
  • NIR-II fluorescence enhancement liposome and preparation method and application thereof
  • NIR-II fluorescence enhancement liposome and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Preparation of fluorescence-enhanced liposome ICG-IR1061-LP

[0041]Accurately weigh lecithin (PC), Tween-80, cholesterol and IR1061 (mass ratio 120:24:12:1), dissolve in a round bottom flask with dichloromethane / ethanol (~15ml) at a volume ratio of 8:1 Internally, 37°C rotary evaporation, after film formation, evacuate in a vacuum pump for 6h, add a certain concentration of ICG solution (PC:ICG=120:1, m / m; ICG dispersed in 0.9% NaCl aqueous solution), 37°C, 130rpm Oscillating hydration for 3 hours, ultrasonication in a water bath for 3 minutes, and passing through 450nm and 220nm syringe filter membranes twice to obtain the filtrate to prepare NIR-II imaging liposomes (IR1061 Liposome), and the final concentration of the liposome solution is 9mg PC / mL . The liposome solution was placed in a 200KDa dialysis bag and dialyzed overnight at room temperature to remove free ICG to prepare fluorescence-enhanced liposome ICG-IR1061-LP.

[0042] The ICG concentration of the fl...

Embodiment 2

[0044] Preparation of fluorescence-enhanced liposome ICG-IR1061-CLP

[0045] After accurately weighing lecithin (PC), Tween-80, cholesterol, octadecylamine and IR1061 (mass ratio 120:24:12:12:1) according to the formula, use dichloromethane / ethanol with a volume ratio of 8:1 (~15ml) dissolved in a round-bottomed flask, 37 ℃ rotary evaporation, after film formation, evacuate in a vacuum pump for 6h, add a certain concentration of ICG solution (PC:ICG=120:1, m / m; ICG dispersed in 0.9% NaCl aqueous solution), 37°C, 130rpm shaking hydration for 3h, water bath ultrasonication for 3min, respectively passing through 450nm and 220nm syringe filter twice to obtain the filtrate, and make NIR-II imaging liposome (IR1061 Liposome), lipid The final concentration of the body solution was 9mg PC / mL. The liposome solution was placed in a 200KDa dialysis bag and dialyzed overnight at room temperature to remove free ICG to prepare fluorescence-enhanced liposome ICG-IR1061-CLP.

[0046] The IC...

Embodiment 3

[0054] In order to evaluate the NIR-II fluorescence performance of the fluorescence-enhanced liposome ICG-IR1061-LP obtained in Example 1. Dilute the fluorescence-enhanced liposome ICG-IR1061-LP to 7.5 μg / mL of IR1061, measure the NIR-II spectrum of the liposome by a low-temperature time-resolved fluorescence spectrometer, and analyze its fluorescence with ICG-LP and IR1061-LP as the control group Spectral changes, such as Figure 4 As shown, the fluorescence of IR1061-LP (identified as IR1061 in the figure) and ICG-IR1061-LP (identified as ICG-IR1061 in the figure) are significantly stronger than ICG-LP (identified as ICG in the figure) near infrared Two-zone fluorescence. Compared with IR1061-LP, while the fluorescence intensity of ICG-IR1061-LP is further enhanced, the fluorescence emission peak is red-shifted (~50nm)

[0055] Put the same concentration of IR1061 liposomes and ICG-IR1061-LP solution into 0.5mL capped centrifuge tubes (1.6, 3.2, 4.8, 6.4, 8mg PC / mL), and u...

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Abstract

The invention discloses an NIR-II fluorescence enhancement liposome and a preparation method and application thereof. The liposome film of the NIR-II fluorescence enhancement liposome contains an NIR-II fluorescent probe IR1061, and indocyanine green is wrapped in liposome watercore. The preparation method comprises the steps of enabling components of the liposome film to disperse in an organic solvent, performing rotary evaporation and film formation, removing remaining organic solvents, adding the indocyanine green solution, and performing hydration. The NIR-II fluorescence enhancement liposome is free from inorganic nanometer materials, through co-carrying of ICG and IR1061, the fluorescence enhancement liposome which is notably higher than single components is obtained, and is higher than the IR1061 liposome (about 4 times), and a liposome emission peak causes red shift; and the fluorescence intensity of the NIR-II fluorescence enhancement liposome is greatly improved, so that required fluorescent imaging can be realized within low-dose low-power conventional exposure time.

Description

technical field [0001] The invention belongs to the field of nanotechnology and fluorescence diagnosis research, and in particular relates to a NIR-II fluorescence enhanced liposome and its preparation method and application. Background technique [0002] Changes in hemodynamic information are closely related to many diseases. Vascular imaging can provide real-time information of anatomy and hemodynamics, and promote the progress of diagnosis and treatment of vascular diseases, such as peripheral vascular disease and traumatic brain injury, which has important clinical significance. Compared with other imaging methods, fluorescence imaging has the advantages of simple operation, high sensitivity and no radioactive pollution. Fluorescence imaging is easy to operate and the characteristics of portable and movable imaging equipment make fluorescence imaging suitable for use as an imaging guidance technique in surgery. However, the limited resolution and imaging depth of the t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K9/127A61K47/18
CPCA61K49/0084A61K49/0034A61K49/0052
Inventor 吉民王子安蔡进刘洋陈峻青
Owner SOUTHEAST UNIV
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