The invention discloses an NIR-II region fluorescent probe suitable for
intrathecal injection, a preparation method of the NIR-II region fluorescent probe and application of the NIR-II region fluorescent probe in cerebrovascular imaging, and belongs to the technical field of probe preparation. The problem of
low resolution of current cerebrovascular imaging is solved. The
rare earth nano fluorescent material is prepared into the NIR-II region fluorescent probe, delivery of large-size
fluorescent imaging particles is achieved through
intrathecal injection, and then cerebral
vascular imaging is achieved. According to the
rare earth nano fluorescent material, NaErF4: x% Ce serves as a
fluorescence emission core, NaYF4: y% Yb serves as a
fluorescence emission middle layer, NaYF4 serves as a
fluorescence emission shell layer, through Er < 3 + >
ion self-
sensitization, under 808 nm excitation, an NIR-II region fluorescence emission peak of 1525 nm is achieved, NaYF4 serves as an
inert shell layer, the
luminous intensity of the fluorescent material is further improved, and NIR-II region high-resolution fluorescence imaging of mouse cerebral vessels is achieved in combination with
intrathecal injection.