31 results about "Sentinel lymph node" patented technology

The invention relates to the technical field of medical instruments, and discloses a breast cancer sentinel lymph node positioning system and method, and the system comprises a data collection subsystem, a space positioning subsystem and a central processing subsystem. The method comprises the following steps: acquiring an anatomical structure image, a radioactivity counting rate and fluorescence intensity after performing primary fluorescence, radioactivity and secondary pulse type fluorescence tracer injection in sequence; establishing a global coordinate system and generating an anatomical region of interest; constructing a weighted directed topological graph based on the initial fluorescence data, and obtaining a baseline flow velocity; extracting radionuclide dynamic characteristics based on the radioactive data; a functional flow rate attenuation coefficient is calculated based on the second pulsed fluorescence data. And a multi-factor scoring model is applied, topological information, dynamic characteristics and functional flow velocity attenuation coefficients are fused, and a comprehensive score is calculated to identify the sentinel lymph node. According to the method, the lymphatic flow topological structure, nuclide dynamics and functional flow velocity information are combined, and the positioning accuracy and reliability are improved.

The invention discloses a frequency mixing bias magnetic nanoparticle magnetization sensing distance measurement method and device, belongs to the technical field of magnetic sensing detection, and solves the problems that the distribution density of magnetic lines is quickly reduced along with the increase of spatial distance, and the concentration information is inaccurately restored under the condition that the distance is unknown in the prior art. According to the frequency mixing bias magnetic nanoparticle magnetization sensing distance measurement method and device, on the premise that a large number of coils are not additionally arranged, the space distribution of an excitation magnetic field near a sensor, the nonlinear magnetization characteristics of a superparamagnetic signal, the magnetization response distance and direction of a superparamagnetic particle and the like are analyzed in detail; the one-dimensional chromatography detection in the lymph node detection direction is innovatively realized, the depth and concentration information of the tracer agent can be synchronously detected and restored, and the detection accuracy of the sentinel lymph node magnetic sensitive detection technology is greatly enhanced.

The present invention discloses a sentinel lymph node ultrasound image semantic segmentation method based on deep learning, which relates to the technical field of image semantic segmentation. First, the present invention obtains a sentinel lymph node ultrasound image data set and preprocesses the data set to obtain a training set; then constructs a RA-U-Net++ network for image semantic segmentation, and tunes the network parameters based on the training set to obtain a medical image semantic segmentation model for target lesions; the network adopts a multi-layer U-shaped structure, and an encoding node using a residual atrous pyramid module is set at each level. The output feature map of the encoding node at the current level is downsampled and then input into the encoding node at the next level; decoding nodes are set from the first layer to the penultimate layer, and the number of decoding nodes decreases layer by layer. The decoding node at each level is located behind the encoding node, and the input of each decoding node includes the output feature maps of all previous nodes and the output feature map of the previous adjacent node in the next level; the final segmentation result is obtained based on the output feature map of the last decoding node in the first layer. The present invention realizes the automatic segmentation of sentinel lymph node ultrasound images, getting rid of the cumbersome, time-consuming and laborious manual segmentation; and has a good segmentation effect, and can obtain a high intersection over union IoU.

The present invention discloses a method, system and storage medium for predicting sentinel lymph node metastasis in breast cancer. The method includes: obtaining labeled WSIs as a training data set, and performing preprocessing to obtain an image patch set; constructing a WSI classification model; using the image patch set to pre-train a feature extractor to obtain a feature vector set; inputting the feature vector set into a prototype clustering module, and extracting multiple prototypes through clustering; dividing the sentinel lymph node WSI of breast cancer into image patches and inputting them into the feature extractor to extract image patch features; inputting the image patch features and prototypes into a feature fusion module for matching to generate a soft assignment histogram, and constructing a feature vector of the sentinel lymph node WSI of breast cancer; sending the feature vector of the sentinel lymph node WSI of breast cancer into a fully connected layer to obtain a WSI classification score and perform metastasis judgment. This method can better solve the problem of micro-metastasis recognition while maintaining accurate recognition of macro-metastasis, so as to accurately diagnose sentinel lymph node metastasis in breast cancer.

The utility model relates to the field of medical instruments, in particular to a quick-release gamma ray detector probe which comprises a handle and a probe body. The handle comprises a handle body and a control panel, the control panel is arranged on the side face of the handle body, and the control panel is used for controlling the probe to conduct gamma ray detection analysis; the front end of the handle is provided with a Remo joint socket; the probe comprises a Remo joint plug, a fixed long rod and a probe; long rods with included angles are arranged at the two ends of the fixed long rod; the Remo joint plug is arranged at the tail part of the fixed long rod, and the probe is arranged at the front end of the fixed long rod; the Remo joint socket and the Remo joint plug are matched with each other; the probe is connected to the Remo connector plug through a wire arranged in the fixed long rod, and is finally connected to the control panel through cooperation of the Remo connector plug and the Remo connector socket. The probe is convenient to disassemble and split, and can be quickly disinfected before sentinel lymph node detection.

The application belongs to the technical field of pharmaceutical preparations, and particularly relates to an indocyanine green preparation and a preparation method and application thereof. The preparation is a liquid preparation or a freeze-dried preparation, raw materials for preparing the preparation include indocyanine green, phospholipid, cholesterol, a freeze-drying protective agent, water and a pH regulator, the pH regulator is selected from one or more of sodium citrate, potassium citrate, sodium tartrate, sodium acetate, sodium lactate and sodium succinate, and the pH of the preparation is adjusted to 6.0-8.5. By means of a specific organic acid salt buffer system and a specific preparation process (the freeze-drying protective agent is added before lipid film deposition), the application can specifically inhibit the growth of impurities with RRT 0.7 and RRT 0.3, respectively, and significantly improve the chemical stability of the preparation. Meanwhile, the preparation can realize accurate positioning of sentinel lymph nodes and second and third lymph nodes, and can be used for preparing a lymph tracer. The application effectively solves the technical problems of rapid impurity growth, poor stability and inaccurate multi-stage lymph positioning of the existing ICG preparation.

The invention discloses a sentinel lymph node nano tracer and a preparation method and application thereof. The sentinel lymph node nano tracer is prepared from the following raw materials: a quantum dot material with near-infrared two-region luminescence characteristic, an amphiphilic polymer, a catalyst, a diamino polymer, a macrophage CD206 receptor targeting molecule and a temperature-sensitive polymer. Wherein the carboxyl group ratio of the macrophage CD206 receptor targeting molecule to the temperature sensitive polymer is (500 to 1000): (100 to 5000). The nano tracer integrates the high-resolution imaging advantage of quantum dots, the temperature response type aggregation characteristic and the receptor-mediated targeted delivery mechanism, can effectively prolong the developing time window while realizing accurate positioning of sentinel lymph nodes, and can provide a new design idea for developing intelligent response type surgical navigation probes; good application prospects are realized.

The application relates to the technical field of medical devices, in particular to a skin ultrasound contrast-based axillary sentinel lymph node puncture guiding method. The core of the method is as follows: after injecting an ultrasound contrast agent with a specific particle size through the skin, a preset low-mechanical-index high-frame-rate double-amplitude contrast imaging mode is adopted to dynamically track lymph drainage and sentinel lymph node development, and the enhancement characteristics are recorded; then, under the guidance of multimodal imaging which is fused with real-time contrast, elastic imaging and color Doppler, the target lymph node is accurately punctured and biopsied, and a tissue sample is obtained for pathological analysis. The application realizes integrated operation from sensitive development of the sentinel lymph node, function evaluation to accurate puncture, and provides a non-radiation high-precision puncture guiding method for accurate evaluation of the axillary lymph node state of breast cancer patients before operation.

The utility model relates to the technical field of tracer injection, and discloses a sentinel lymph node tracer injector which comprises a needle cylinder, a needle head assembly is inserted into the bottom end of the needle cylinder, the needle head assembly comprises a needle head sleeve and an injection needle head, and the injection needle head is fixedly installed at the bottom of the needle head sleeve. According to the sentinel lymph node tracer injector, when a piston is controlled by an auxiliary pull rod to extract a tracer, a fixing ring fixedly connected with the surface of the auxiliary pull rod drives sliding blocks on the two sides of a pointing lever fixedly connected with a linkage arm to slide in two sets of sliding sleeve shells; in the sliding process, the extraction amount can be clearly known through comparison of the pointing lever and the milliliter line, meanwhile, one of the limiting grooves formed in one set of sliding sleeve shells is aligned with the milliliter line needing milliliter extraction, and then a limiting insertion rod is inserted to form limiting; and therefore, the single extraction amount is quantified in the extraction process, and excessive extraction is avoided.

The present invention provides a near-infrared-II (NIR-II, 1000-1700 nm) chemiluminescence resonance energy transfer (CRET) nanosystem, a preparation method therefor, and a use thereof. The NIR-II CRET nanosystem is constructed by means of multi-component synergistic self-assembly of a pH-responsive amphiphilic short peptide, a metal ion, an NIR-II CRET functional unit, and a photosensitizer, and has an NIR-II CRET signal activated by responding to both pH and MPO enzyme in a microenvironment. The NIR-II CRET nanosystem can be used as a diagnostic reagent to achieve accurate tracing and localization of a sentinel lymph node of breast cancer by means of an NIR-II contrast agent. The nature of the sentinel lymph node is determined by means of the NIR-II CRET signal activated by responding to both pH and MPO enzyme, thereby overcoming the technical problem that it is difficult for conventional sentinel lymph node diagnosis methods to avoid false negatives and false positives, and achieving in-vitro localization and qualitative integrated diagnosis and treatment of a metastatic sentinel lymph node of breast cancer.

Disclosed herein is a method comprising introducing a tracer into a body region of a living organism at an introduction site; causing emission of characteristic X-rays of the tracer in the body region (710); taking an image of the tracer in the body region with the characteristic X-rays (720); determining a first three-dimensional (3D) distribution of the tracer in the body region based on the image (730); and examining the first 3D distribution of the tracer in the body region to identify a sentinel lymph node of the introduction site. If the sentinel lymph node is not identified in the first 3D distribution, the method further comprises repeating the foregoing causing emission of characteristic X-rays of the tracer in the body region, the foregoing taking an image of the tracer in the body region, and the foregoing determining a 3D distribution of the tracer in the body region to produce a second 3D distribution of the tracer in the body region; and examining the second 3D distribution to identify the sentinel lymph node.

The invention discloses a malignant melanoma sentinel lymph node biopsy system, method and application based on SPECT-Hololens. A preoperative preparation module comprises a data acquisition module, a three-dimensional reconstruction module and a preoperative planning module; the data acquisition module is used for at least acquiring SPECT image data of a patient; the three-dimensional reconstruction module is used for generating a three-dimensional model; the preoperative planning module is used for observing the three-dimensional model through HoloLens, determining the position of a sentinel lymph node and planning an operation path; the intraoperative operation module comprises a HoloLens head-mounted device worn by a doctor, a registration module and a positioning module, a three-dimensional model superposed on a patient is observed through HoloLens, the three-dimensional model is navigated to a sentinel lymph node position for marking and positioning in real time, and accurate positioning information is provided under the guidance of mixed reality; the postoperation processing module is used for assisting postoperation detailed analysis and collecting key data including operation time, intraoperative details, operation type improvement, experience action improvement and the like.

The invention provides a breast cancer sentinel lymph node positioning device, and belongs to the technical field of medical equipment. According to the device, a light absorption substance is injected through the injection module, the laser emission module emits a laser beam of which the wavelength is matched with the absorption peak of the light absorption substance, and the infrared thermal imaging module captures the temperature change caused by the photothermal effect and displays the overall design of an infrared thermal image. The sentinel lymph node positioning device solves the problem that an existing device using radionuclide combined with a blue dye method cannot position sentinel lymph nodes visually and accurately in real time without radiation. The device comprises an injection module, a laser emission module and an infrared thermal imaging module, and further comprises a temperature control module for controlling the temperature of an irradiation area of the laser emission module to be lower than a set temperature threshold value; the position adjusting module is used for adjusting the position of the laser emitting module; and the control module is used for controlling the laser emitting module, the infrared thermal imaging module and the position adjusting module.

The present invention provides gene signatures for classifying individuals having skin melanoma. The "SLN gene signatures" provided herein classify individuals based on prognosis and / or classify individuals as having metastasis-positive or -negative sentinel lymph nodes (SLNs). The "N-SLN gene signatures" provided herein classify individuals as having metastasis-positive or -negative non-sentinel lymph nodes (N-SLNs).

The invention relates to the technical field of medical instruments, in particular to an armpit sentinel node needle biopsy method based on skin ultrasound contrast. The core of the method is that after an ultrasonic contrast agent with a specific particle size is injected percutaneously, lymphatic drainage and sentinel lymph node development are dynamically tracked by adopting a preset low-mechanical-index and high-frame-rate double-amplitude contrast imaging mode, and enhancement characteristics of the lymphatic drainage and sentinel lymph node development are recorded; furthermore, under the guidance of multi-mode imaging fusing real-time radiography, elastography and color Doppler, precise needle biopsy is performed on the target lymph node, and a tissue sample is obtained for pathological analysis, so that integrated operation from sensitive development and function evaluation of the sentinel lymph node to precise puncture is realized; and a non-radiation and high-precision minimally invasive diagnosis means is provided for accurate evaluation of the preoperative axillary lymph node state of a breast cancer patient.

The provided is a system that provides information with high accuracy by performing voxel-based both cross-sectional and volumetric three-dimensional imaging in cancer tissues, especially in determining the necessary surgical margins of the breast tumor removed from the patient with breast cancer surgery and the presence of the tumor in the sentinel lymph node, which is the first lymph node from which the tumor drained. The provided will not be applied directly to humans and the human body, but to the tumor and / or tumorous tissue removed from the patient's cancerous organ (especially the breast). By changing the software parameters and the applied X-ray range used in breast tumors, a deep technology-based system developed can also be used in human lung, liver, pancreas, thyroid, brain, skin, small intestine, large intestine, stomach, gall bladder, esophagus, bladder, kidney, ovary, uterus, cervix, testis and prostate tumors and tumorous tissue fragments.

The present invention discloses an oxy-phenothiazine salt compound, its preparation method, and application. The present invention adopts the "heavy atom-free effect" and utilizes the bridging sulfur atom of the oxygen-substituted phenothiazine salt to perform homologous atom substitution to develop a novel fluorescent dye, an oxy-phenothiazine salt (MBO). The oxy-phenothiazine salt of the present invention reduces the spin-orbit coupling (SOC) constant of methylene blue (MB) and the intersystem crossing rate (k) from the singlet state to the triplet state. ISC ), enhancing its fluorescence imaging capabilities and offering improved safety and lower cytotoxicity compared to methylene blue itself. The novel fluorescent dye of the present invention combines blue staining of lymph nodes with fluorescence imaging, making it suitable for use in the preparation of reagents for sentinel lymph node biopsy, intraoperative lymph node tracing, and clearance.

The disclosure provides gene signatures of classifying an individual afflicted with cutaneous melanoma. The “SLN gene signatures” provided herein classify an individual based on prognosis and / or classify an individual as having a metastasis-positive or -negative sentinel lymph node (SLN). The “N-SLN gene signatures” provided herein classify an individual as having a metastasis-positive or -negative non-sentinel lymph node (N-SLN).

The disclosure provides gene signatures of classifying an individual afflicted with cutaneous melanoma. The “SLN gene signatures” provided herein classify an individual based on prognosis and / or classify an individual as having a metastasis-positive or -negative sentinel lymph node (SLN). The “N-SLN gene signatures” provided herein classify an individual as having a metastasis-positive or -negative non-sentinel lymph node (N-SLN).

The application provides a near-infrared two-region (NIR-II, 1000-1700 nm) chemiluminescence resonance energy transfer nanosystem and a preparation method and application thereof; the near-infrared two-region chemiluminescence resonance energy transfer nanosystem is constructed by multi-component synergistic self-assembly of a pH-responsive amphiphilic short peptide, metal ions, a NIR-II CRET system and a photosensitizer, has a NIR-II CRET signal activated by double responses of pH and MPO enzymes in a microenvironment, can be used as a diagnostic reagent to realize accurate tracking and positioning of breast cancer sentinel lymph nodes through a near-infrared two-region contrast agent, realizes the qualitative determination of sentinel lymph nodes through the double-response activated NIR-II CRET signal, and thus overcomes the technical difficulties that the traditional sentinel lymph node diagnostic method cannot avoid false negative and false positive.

This invention discloses an activated near-infrared II afterglow luminescence composite nanosystem, its preparation method, and its applications. The activated near-infrared II afterglow luminescence composite nanosystem is assembled from visible afterglow nanoparticles, near-infrared II quantum dots, amphiphilic molecules, matrix metalloproteinase-responsive peptides, and fluorescence quenchers. This invention integrates visible afterglow nanoparticles and near-infrared II quantum dots to construct a radiation energy transfer system, achieving long afterglow luminescence in the near-infrared II region. This afterglow signal can be specifically activated by matrix metalloproteinases (MMPs) highly expressed in the breast cancer microenvironment, achieving a state transition from "off" to "on," enabling precise detection of metastatic sentinel lymph nodes in breast cancer.

The invention discloses a novel application of indigo carmine in preparation of a medicine for draining lymph nodes in a tracing area during a malignant tumor treatment operation, and belongs to the technical field of medicines. Aiming at the technical problems of pigmentation, tissue corrosivity and other side injuries of an existing lymph node tracer, animal experiments prove for the first time that indigo carmine with a specific dosage is adopted to trace a tumor area to drain lymph nodes, so that the side effects of the existing tracer can be remarkably reduced, and the curative effect of the tracer is improved. Meanwhile, the detection effect on target lymph nodes is similar to that of the existing common tracer agent methylene blue. The invention provides clear experimental evidence, the known safe drug indigo carmine injection is repositioned to be used for accurately tracing the regional lymph node in the malignant tumor operation, the sentinel lymph node biopsy is guided, the side injury caused by blind enlargement of the operation cleaning range is avoided, and the method has the advantages of short development period, definite tracing effect, low cost and the like. The invention provides a drug choice with clinical application potential for precise and minimally invasive surgery of various malignant tumors.

The invention relates to a preparation method of a breast cancer sentinel lymph node metastasis prediction auxiliary agent based on circulating tumor cell phenotype analysis, and belongs to the field of biomedical detection. The auxiliary agent comprises specific antibody magnetic beads, a cell lysis solution, a nucleic acid extraction reagent, a fluorescence labeling probe and a phosphate buffer solution; the preparation method covers the specific preparation steps of all the components, efficient enrichment, nucleic acid extraction and visual phenotype analysis of circulating tumor cells can be achieved through the auxiliary, and the auxiliary has high sensitivity and specificity and is stable in reaction system. Meanwhile, serine protease and asiaticoside in the auxiliaries play an important role in accelerating the hydrolysis process of tetraethoxysilane, optimizing the surface charge distribution of the magnetic nanoparticles, enhancing the recognition capability of the magnetic beads on tumor cells, improving the stability of the magnetic nanoparticles in a complex biological system and the like; powerful support can be provided for prediction of breast cancer sentinel lymph node metastasis, and formulation of a clinical treatment scheme is assisted.

The present invention discloses a device for locating sentinel lymph nodes in breast cancer. The locating device includes a laser emitter and a probe device covered on the laser emitter. The probe device includes a flexible circuit substrate and multiple groups of piezoelectric micro-ultrasonic transducers arranged in an array on the flexible circuit substrate. Two adjacent groups of piezoelectric micro-ultrasonic transducers are spliced ​​together. Each piezoelectric micro-ultrasonic transducer includes multiple ultrasonic units. Each ultrasonic unit encloses a housing space. The housing space contains a lead zirconate titanate block bonded to the ultrasonic unit. By integrating the lead zirconate titanate block and the piezoelectric micro-ultrasonic transducer on the flexible circuit substrate, the probe device can fully cover the breast lymphatic drainage area, implement dynamic tracking of the trajectory of the lymph node tracer in the lymphatic vessels, realize dynamic tracking of the entire process, and accurately identify and locate the position of the sentinel lymph node.

The present invention discloses a highly efficient near-infrared zone II aggregation-induced luminescence material, a preparation method thereof, and its application in dual-modality surgical navigation. This type of molecular structure uses thionaphthalenetetracarboxylic acid diimide as an electron acceptor group and tetraphenylethylene as a molecular rotor and electron donor unit to construct an organic near-infrared zone II material with intramolecular charge transfer characteristics. By expanding the length of the π-conjugated fragment and introducing the isotope effect, the oscillator strength can be increased and high-frequency vibrations can be suppressed, thereby promoting the radiative transition rate of the molecule and suppressing the non-radiative transition rate. The nanoparticles prepared using the above-mentioned aggregation-induced luminescence material have the advantages of high brightness, large Stokes shift and good photostability, and have excellent effects in the surgical resection of in situ breast cancer and sentinel lymph nodes in mice guided by dual-modality imaging.

The present application belongs to the technical field of image processing, and particularly relates to a breast cancer lymph node benignity and malignancy classification system and method based on preoperative and intraoperative images, and aims to solve the problem of breast cancer lymph node error cleaning in the prior art. The present application comprises: a preoperative multi-modal image preprocessing and coding module, which extracts lymph node related high-dimensional pre-defined features of a patient's preoperative multi-modal image; an intraoperative fluorescent image sequence preprocessing and coding module and an intraoperative fluorescent intensity time-varying sequence preprocessing and coding module, which respectively extract global features and local features of intraoperative lymph nodes in fluorescent images; an intraoperative sentinel / non-sentinel lymph node benignity and malignancy classification module, which fuses the high-dimensional pre-defined features, global features and local features, and performs modeling and model training, and classifies breast cancer lymph nodes according to benignity and malignancy through the trained model. The present application has high accuracy and precision of analysis results, and can provide a better auxiliary diagnosis and treatment scheme.

The invention relates to the field of medical instruments, in particular to an automatic breast massage device for sentinel lymph node biopsy, which comprises a device main body, the device main body comprises an arc-shaped support cup, a fixing module and a control module, the arc-shaped support cup is fixedly connected with the fixing module, and a massage module is mounted on the arc-shaped support cup; the massage module comprises a mounting seat, a plurality of massage assemblies and a driving assembly, and the massage assemblies are fixedly connected with the mounting seat; an elastic adaptation assembly and a tracer diffusion auxiliary assembly are further installed in the arc-shaped supporting cup, the driving assembly, the elastic adaptation assembly and the tracer diffusion auxiliary assembly are in signal connection with the control module, and the control module controls the driving assembly according to a preset massage program and real-time data fed back by the elastic adaptation assembly and the tracer diffusion auxiliary assembly. And then the massage assembly is driven to massage the patient. The lymph node dyeing definition and dyeing efficiency are improved, traditional manual massage is replaced, and meanwhile individual differences of different patients are adapted.

The present invention discloses a retractor for axillary sentinel lymph node biopsy, which relates to the technical field of medical devices. It includes a central connecting plate, one end of the central connecting plate is fixedly connected to a driving front rod, the other side of the driving front rod is fixedly connected to a spreading bent rod, two ends of the spreading bent rod are fixedly connected to mounting blocks, and one end of the mounting block is fixedly clamped with a spreading side retractor. The present invention effectively improves the spreading area of ​​the sentinel lymph node wound by medical staff, effectively improves the surgical field of view, increases the operating space, and the surgeon can complete the operation alone. At the same time, the flexible structural design of the elastic connecting plate and the lateral spreading retractor effectively prevents additional lacerations caused by rigid pulling on the skin edge when the wound is longitudinally spread, further improving the safety of the use of the retractor.

The utility model relates to the field of detection devices, in particular to a sentinel lymph node detection gamma ray detection device which comprises a handle, a detection rod and a probe which are sequentially connected. The detection rod comprises a first detection rod and a second detection rod which are connected in sequence; wherein the first detection rod is positioned at one end close to the probe and is of a gooseneck structure; the second detection rod is of a telescopic structure. The whole detection rod is divided into the telescopic section and the direction-adjustable section, during use, the distance and the direction from a probe to a sentinel lymph node can be adjusted at any time by adjusting the bending angle of the first detection rod and the length of the second detection rod according to the actual condition of an operation, great convenience is brought to finding of the lymph node, the operation difficulty is reduced, and the operation efficiency is improved. The detection efficiency is effectively improved, the structure is simple, and popularization and utilization are convenient.

Methods of predicting the development of a metastasis in a subject suffering from cancer wherein a sentinel lymph node with respect to the cancer does not comprise cancer cells are provided. Method of predicting the absence of development of a metastasis in a subject suffering from cancer which a sentinel lymph node with respect to the cancer does comprise cancer cells are also provided.