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Specific sentinel lymph node developer drug and preparation method thereof

A sentinel lymph node and imaging agent technology, applied in the direction of radioactive carriers, can solve the problems of high radioactive retention at the injection point, limited time for imaging and biopsy, etc., and achieve the effect of low retention at the injection point

Inactive Publication Date: 2010-12-29
BEIJING CANCER HOSPITAL PEKING UNIV CANCER HOSPITAL
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Problems solved by technology

These drugs have drawbacks such as limited time for secondary lymph node uptake, imaging and biopsy, and high radioactive retention at the injection site

Method used

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  • Specific sentinel lymph node developer drug and preparation method thereof
  • Specific sentinel lymph node developer drug and preparation method thereof
  • Specific sentinel lymph node developer drug and preparation method thereof

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Embodiment

[0028] (1) Antibody modification Add 2-mercaptoethanol to the Rituximab antibody (the molar ratio of the two substances is 1:1000), react in the dark for 15 minutes, and then separate with a PD-10 column.

[0029] (2) Preparation of freeze-dried kits The separated antibodies were subpackaged, each containing 0.5mg of antibody, and 20mg of glucose excipient, Dura-Dry TM Freeze drying in a freeze dryer. That is, the freeze-dried kit of Rituximab.

[0030] (3) For radioactive labeling, add 0.1ml of PBS to the lyophilized kit to dissolve, then sequentially add 1mg of glucoheptose, 15μg of stannous chloride, and 370MBq of Na 99m TCO 4 , after reacting at room temperature for 10 minutes, the PD-10 column was separated to obtain 99m Tc-Rituximab. Determination of labeling rate and radiochemical purity.

[0031] The determination of the labeling rate adopts double-layer chromatography to develop and measure, system 1: ITLC-SG (fast silica gel thin layer chromatography-silica gel ...

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Abstract

The invention provides a specific sentinel lymph node developer drug and a preparation method thereof. A Rituximab antibody is modified and subject to 99mTc marking, so that 99mTc-Rituximab can be obtained, which can combine with a CD20 antigen in the sentinel lymph node to accurately position the sentinel lymph node, thus the sentinel lymph node development of the breast cancer and a biopsy analysis can be performed on breast cancer patients, thereby laying a foundation for establishing a complete set of safe, convenient and effective sentinel lymph node development technology of breast cancer and providing a method and basis for widely clinical application in the coming days.

Description

technical field [0001] The invention relates to a specific sentinel lymph node imaging agent drug and a preparation method thereof, in particular to a Rituximab antibody-labeled specific sentinel lymph node imaging agent drug and a preparation method thereof. Background technique [0002] Sentinel lymph node (sentinel lymph node, SLN) is the first lymph node through which the primary cancer undergoes lymph node metastasis. The sentinel lymph node will first accommodate tumor cells that transfer along the lymphatic drainage pathway, thus reflecting the tumor in the entire local lymphatic drainage area. situation. Sentinel lymph node biopsy (sentinel lymph node biopsy, SLNB) refers to the technical means of obtaining sentinel lymph nodes and conducting pathological examination. Generally speaking, the absence of tumor metastasis in the sentinel lymph nodes can exclude the absence of spread in the lymphatic drainage area. [0003] In the treatment of breast cancer, axillary l...

Claims

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Application Information

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IPC IPC(8): A61K51/10A61K103/10
Inventor 杨志李艳王雪娟
Owner BEIJING CANCER HOSPITAL PEKING UNIV CANCER HOSPITAL
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